期刊
BIOMEDICINES
卷 8, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/biomedicines8080268
关键词
fecal microbiota transplantation (FMT); inflammatory bowel disease (IBD); ulcerative colitis (UC); gut microbiota
资金
- Poznan University of Medical Sciences, Poznan, Poland [502-01-222-38-000]
The modification of the microbiome through fecal microbiota transplantation (FMT) is becoming a very promising therapeutic option for inflammatory bowel disease (IBD) patients. Our pilot study aimed to assess the effectiveness of multi-session FMT treatment in active ulcerative colitis (UC) patients. Ten patients with UC were treated with multi-session FMT (200 mL) from healthy donors, via colonoscopy/gastroscopy. Patients were evaluated as follows: at baseline, at week 7, and after 6 months, routine blood tests (including C reactive protein (CRP) and calprotectin) were performed. 16S rRNA gene (V3V4) sequencing was used for metagenomic analysis. The severity of UC was classified based on the Truelove-Witts index. The assessment of microbial diversity showed significant differences between recipients and healthy donors. FMT contributed to long-term, significant clinical and biochemical improvement. Metagenomic analysis revealed an increase in the amount ofLactobacillaceaea,Micrococcaceae,Prevotellaceae, andTM7 phylumsp.oral clone EW055during FMT, whereasStaphylococcaceaeandBacillaceaedeclined significantly. A positive increase in the proportion of the generaBifidobacterium,Lactobacillus,Rothia,Streptococcus, andVeillonellaand a decrease inBacillus,Bacteroides, andStaphylococcuswere observed based on the correlation between calprotectin andBacillusandStaphylococcus;ferritin andLactobacillus,Veillonella,andBifidobacteriumabundance was indicated. A positive change in the abundance ofFirmicuteswas observed during FMT and after 6 months. The application of multi-session FMT led to the restoration of recipients' microbiota and resulted in the remission of patients with active UC.
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