Article
Plant Sciences
Pornpatsorn Lertphadungkit, Xue Qiao, Min Ye, Somnuk Bunsupa
Summary: This study identified two key amino acids of isomultiflorenol synthase and characterized three new OSCs in Trichosanthes cucumerina L., expanding the understanding of triterpenoid biosynthesis in plants. The research provides insights into the catalytic mechanism of plant OSCs that produce specific products.
Article
Biochemistry & Molecular Biology
Hajer Ben Ghozlen, Sven Mangelinckx, Stefaan P. O. Werbrouck
Summary: Stilbenes, including resveratrol, are bioactive compounds in peanuts. This study investigates the production of these compounds from Arachis hypogaea using in vitro systems, specifically callus cultures and cell suspension cultures. Biotic elicitors from various fungi were found to enhance the production of stilbenes, indicating a potential method for production and a new opportunity for obtaining resveratrol and its derivatives.
PROCESS BIOCHEMISTRY
(2023)
Article
Plant Sciences
Pornpatsorn Lertphadungkit, Xue Qiao, Supaart Sirikantaramas, Veena Satitpatipan, Min Ye, Somnuk Bunsupa
Summary: The de novo transcriptome analysis of Trichosanthes cucumerina L. identified genes associated with triterpenoid biosynthesis, particularly bryonolic acid and cucurbitacin B. The study also revealed the presence of isomultiflorenol synthase and cucurbitadienol synthase, important for the production of characteristic triterpenoids in cucurbitaceous plants. This research offers valuable insights for future studies on cucurbitaceous triterpenoids.
PLANT CELL REPORTS
(2021)
Article
Plant Sciences
Eveline Y. Y. Kong, Julianne Biddle, Sundaravelpandian Kalaipandian, Steve W. W. Adkins
Summary: This study attempted to obtain friable coconut embryogenic callus (EC) through various approaches, but none of them were successful. However, some of the culture conditions were found to influence the formation of compact EC, which is important for further studies on somatic embryogenesis in coconut and other species.
Article
Chemistry, Applied
Sunday Edet Etuk, Kokoete Raymond Okokon, Uduak A. Essiet
Summary: This study investigated the chemical and physicochemical properties of Trichosanthes cucumerina seed and seed oil, as well as their fatty acid profile. The seed oil was found to be rich in polyunsaturated fatty acids, making it suitable as an edible vegetable oil and potentially useful in the soap and cosmetic industry.
JOURNAL OF OLEO SCIENCE
(2022)
Article
Agronomy
Lorena de Souza Silva, Daniel Felipe de Oliveira Gentil, Sidney Alberto do Nascimento Ferreira
Summary: This study evaluated seed maturation and germination of Trichosanthes cucumerina L. as a function of age and fruit morphology, as well as the effect of different temperatures on seed germination. The results showed that seeds reached physiological maturity around 50 days after anthesis, and the fruits were partially or completely orange or reddish-orange in color at that time. A temperature of 30 degrees Celsius favored the seed germination process.
JOURNAL OF SEED SCIENCE
(2022)
Article
Multidisciplinary Sciences
Aqeel Ahmad, Muhammad Tahir ul Qamar, Almeera Shoukat, Mehtab Muhammad Aslam, Mohsin Tariq, Mansor Hakiman, Faiz Ahmad Joyia
Summary: The in vitro regeneration and cell suspension culture of chamomile are genotype-dependent. Optimization of culture conditions is crucial for devising tissue culture-based conservation strategy and secondary metabolite production.
Article
Biochemistry & Molecular Biology
Heba Shahin, Atef A. El-Hela, Mahmoud Nasr, Ghada M. Nasr
Summary: Urginea maritima, a medicinal plant at risk of extinction, can effectively synthesize the cardiac glycoside proscillaridin A under specific growth conditions. Suspension cultures of Urginea cells are as productive as callus cultures.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2022)
Article
Plant Sciences
Pattralak Songserm, Poramaporn Klanrit, Poramate Klanrit, Jutarop Phetcharaburanin, Pornthap Thanonkeo, Jirawan Apiraksakorn, Khamphee Phomphrai, Preekamol Klanrit
Summary: This study successfully established callus and cell suspension cultures of Rhinacanthus nasutus and found that the leaf and cell extracts have high antioxidant and anticancer activities. The identification of various compounds in the extracts provides valuable information for further development of phytochemical production using R. nasutus cell suspension culture.
Article
Plant Sciences
Suresh Suryawanshi, Parthraj Kshirsagar, Prajakta Kamble, Vishwas Bapat, Jyoti Jadhav
Summary: The bioprocess methodology for enhanced production of phytochemicals using plant cultures mainly relies on elicitors and precursors, with Mucuna being a key source of L-DOPA. Optimal concentrations of growth promoters, elicitors, and precursors were found to significantly increase the accumulation of L-DOPA and other secondary metabolites in vitro callus culture of Mucuna imbricata. Methyl Jasmonate and Salicylic Acid were particularly effective in enhancing the production of L-DOPA, leading to more than a two-fold increase in content.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Plant Sciences
Chitralekha Shyam, Manoj Kumar Tripathi, Sushma Tiwari, Niraj Tripathi, Ravindra Singh Solanki, Swapnil Sapre, Ashok Ahuja, Sharad Tiwari
Summary: Brassica juncea is a principal oilseed crop of India and Madhya Pradesh, with somaclonal variations explored as a potential source of additional variability for manipulating fatty acids, especially low erucic acid content. Putative somaclones derived from tissue cultures showed varying levels of erucic acid compared to parental lines, providing a potential source of variation for developing future mustard crops with low erucic acid content.
Article
Biotechnology & Applied Microbiology
Reza Farjaminezhad, Ghasemali Garoosi
Summary: Neem is a medicinal plant with various uses, and this study explored the impact of yeast extract and sampling time on cell growth, secondary metabolite synthesis, and gene expression. The results demonstrated that optimal amounts of yeast extract led to increased production of medicinal compounds in a short period of time, with predictions pointing to higher production with long-term exposure to high yeast extract concentrations.
Article
Agronomy
Kleane Targino Oliveira Pereira, Salvador Barros Torres, Emanoela Pereira de Paiva, Tatianne Raianne Costa Alves, Maria Lilia de Souza Neta, Jefferson Bittencourt Venancio, Lauter Silva Souto, Clarisse Pereira Benedito, Tayd Dayvison Custodio Peixoto, Miguel Ferreira Neto, Nildo da Silva Dias, Francisco Vanies da Silva Sa
Summary: Discontinuous hydration cycles can improve the response of maize seeds to saline stress, while water alone failed to enhance the salt stress tolerance of maize seeds. However, discontinuous hydration cycles with gibberellic acid, hydrogen peroxide, and salicylic acid promoted salt stress tolerance in maize.
Article
Plant Sciences
Martin Sak, Ivana Dokupilova, Sarlota Kanukova, Michaela Mrkvova, Daniel Mihalik, Pavol Hauptvogel, Jan Kraic
Summary: In this study, in vitro cell cultures derived from grapevine were induced by different biotic and abiotic elicitors to produce stilbenes. The results showed that cell wall homogenate from F. oxysporum and cellulase from T. viride were more effective in enhancing stilbene production compared to commonly used jasmonates.
Article
Biochemistry & Molecular Biology
Habiba Khan, Tariq Khan, Nisar Ahmad, Gouhar Zaman, Taimoor Khan, Waqar Ahmad, Sannia Batool, Zahid Hussain, Samantha Drouet, Christophe Hano, Bilal Haider Abbasi
Summary: The study demonstrated that AlCl3 at 0.1 mM concentration improved biomass accumulation and increased the accumulation of secondary metabolites, while also enhancing antioxidant enzyme activity. AlCl3 was found to be a better elicitor compared to CdCl2 in terms of productivity and antioxidant enzyme activity.
Article
Plant Sciences
Pawinee Panpetch, Supaart Sirikantaramas
Summary: The study identified DzLAP1 as the main leucylaminopeptidase (LAP) responsible for LAP activity in durian fruit pulp, with DzLAP1 being active in the early stages of fruit ripening. DzLAP1 is a metalloenzyme similar to other LAPs, and shows activity towards its preferred substrate Cys-Gly.
Article
Pharmacology & Pharmacy
Parichart Thummarati, Jiraphong Suksiriworapong, Krisada Sakchaisri, Varaporn Buraphacheep Junyaprasert
Summary: Chemical linkers play a crucial role in determining the physicochemical properties, stability and cytotoxic activities of CUR-HA conjugate nanoparticles. The nanoparticles with pH-sensitive linkers demonstrated better stability, lower drug release at physiological pH, and higher release in acidic cancer cell environments, showing potential for cancer therapy.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2021)
Review
Plant Sciences
Chaiwat Aneklaphakij, Tomoki Saigo, Mutsumi Watanabe, Thomas Naake, Alisdair R. Fernie, Somnuk Bunsupa, Veena Satitpatipan, Takayuki Tohge
Summary: Nuts are valuable food crops rich in fatty acids, vitamins, minerals, and polyphenols, which are beneficial antioxidants. Research on polyphenols in culinary nuts has identified their chemical properties and bioactivities. Advances in whole-genome sequencing have made nut plant species ideal for omics-based approaches.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Pornpatsorn Lertphadungkit, Xue Qiao, Supaart Sirikantaramas, Veena Satitpatipan, Min Ye, Somnuk Bunsupa
Summary: The de novo transcriptome analysis of Trichosanthes cucumerina L. identified genes associated with triterpenoid biosynthesis, particularly bryonolic acid and cucurbitacin B. The study also revealed the presence of isomultiflorenol synthase and cucurbitadienol synthase, important for the production of characteristic triterpenoids in cucurbitaceous plants. This research offers valuable insights for future studies on cucurbitaceous triterpenoids.
PLANT CELL REPORTS
(2021)
Article
Pharmacology & Pharmacy
Kanokporn Damrongrak, Kiattiphant Kloysawat, Somnuk Bunsupa, Krisada Sakchasri, Amaraporn Wongrakpanich, Vincenzo Taresco, Valentina Cuzzucoli C. Crucitti, Martin C. Garnett, Jiraphong Suksiriworapong
Summary: The study demonstrated the fabrication of poly(glycerol adipate) nanoparticles decorated with folic acid and triphenylphosphonium and their potential for delivering acetogenin-enriched leaf extract to ovarian cancer cells. The nanoparticles exhibited small size, narrow size distribution, high negative surface charge, and a biphasic sustained release of the extract. The FOL/TPP-decorated nanoparticles showed higher drug loading, enhanced toxicity to cancer cells, and improved cellular uptake compared to FOL-decorated nanoparticles. The FOL/TPP-NPs also demonstrated better stability, uptake efficiency, and targeting ability to mitochondria.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Parichart Thummarati, Jiraphong Suksiriworapong, Krisada Sakchaisri, Thomas Nawroth, Peter Langguth, Benjamaporn Roongsawang, Varaporn Buraphacheep Junyaprasert
Summary: Dual-delivery nanoparticles of GEM and CUR conjugated on HA were developed for targeted cancer therapy. The nanoparticles showed pH-dependent drug release and exhibited superior characteristics in terms of particle size, size distribution, and critical aggregation concentration. In vitro studies demonstrated enhanced cellular uptake and cytotoxicity in cancer cell lines compared to conventional drug delivery methods.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Plant Sciences
Pornpatsorn Lertphadungkit, Xue Qiao, Min Ye, Somnuk Bunsupa
Summary: This study identified two key amino acids of isomultiflorenol synthase and characterized three new OSCs in Trichosanthes cucumerina L., expanding the understanding of triterpenoid biosynthesis in plants. The research provides insights into the catalytic mechanism of plant OSCs that produce specific products.
Article
Polymer Science
Amaraporn Wongrakpanich, Nichakan Khunkitchai, Yanisa Achayawat, Jiraphong Suksiriworapong
Summary: This study developed ketorolac microparticles using hyaluronic acid stabilized poly(lactide-co-glycolide) (PLGA), poly(lactide) (PLA), and their blend for osteoarthritis treatment. Different emulsification techniques and polymer compositions influenced the microparticle characteristics, with probe sonication resulting in smaller size and broader size distribution than high-speed stirring. The microparticles showed biphasic drug release over 35 days and demonstrated potential for treating osteoarthritis.
Article
Pharmacology & Pharmacy
Jiraporn Leanpolchareanchai, Patamaporn Tangteerakoon, Patcharin Supapsophon, Somsiri Sukavatcharin, Pornchai Simaroj, Jiraphong Suksiriworapong
Summary: This study aimed to prepare extemporaneous 20 mg/mL ganciclovir in artificial tears and compare its stability with that of ganciclovir in sterile water for ophthalmic administration. The results showed that ganciclovir/0.1% HYA eyedrops had good stability at different temperatures and could be used as home medications for the treatment of ophthalmic viral infections.
Article
Biochemistry & Molecular Biology
Nantana Nuchtavorn, Jiraporn Leanpolchareanchai, Satsawat Visansirikul, Somnuk Bunsupa
Summary: In this study, novel magnetic nanoparticles Fe3O4@MIPs and filter paper@MIPs were synthesized by sol-gel polymerization for selective extraction of charantin. Compared to traditional liquid-liquid extraction, these methods exhibited excellent extraction efficiency and selectivity, and were rapid, inexpensive, simple, reproducible, and environmentally friendly.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Jiraphong Suksiriworapong, Nutthachai Pongprasert, Somnuk Bunsupa, Vincenzo Taresco, Valentina Cuzzucoli Crucitti, Thitapa Janurai, Pornpoj Phruttiwanichakun, Krisada Sakchaisri, Amaraporn Wongrakpanich
Summary: This study aimed to enhance the anticancer effect of Cordyceps militaris herbal extract (CME) on breast cancer cells by using hyaluronic acid (HYA) surface-decorated lipid polymer hybrid nanoparticles (LPNPs), and evaluate the feasibility of using a synthesized poly(glycerol adipate) (PGA) polymer for LPNP preparation. The results showed that the synthesized polymers could successfully encapsulate CME in LPNPs. The HYA-decorated PGA-based LPNPs significantly improved the anticancer effect of CME against breast cancer cells by enhancing cellular uptake through CD44 receptor-mediated endocytosis.
Article
Pharmacology & Pharmacy
Jiraphong Suksiriworapong, Chittin Achayawat, Phutthikom Juangrattanakamjorn, Vincenzo Taresco, Valentina Cuzzucoli Crucitti, Krisada Sakchaisri, Somnuk Bunsupa
Summary: This study aimed to fabricate new variations of glycerol-based polyesters by grafting poly(glycerol adipate) (PGA) with hydrophobic bioactive moieties, tocopherol (TOC), and cholesterol (CHO). Their effects on nanoparticle (NP) formation, drug release, and cellular responses in cancer and normal cells were evaluated. The results showed that both PGA-TOC NPs and PGA-CHO NPs could be used as delivery systems for anticancer drugs, with different characteristics and effects on cancer and normal cells.
Review
Biochemistry & Molecular Biology
Chaiwat Aneklaphakij, Phatthilakorn Chamnanpuen, Somnuk Bunsupa, Veena Satitpatipan
Summary: Stilbenoids, well-known phytoalexins, have various bioactivities and are widely used in cosmetic products. However, the supply of stilbenoids from plants is limited and traditional organic solvent extraction methods are harmful to the environment and human health. Therefore, researchers are focusing on green technologies to achieve sustainable stilbenoids production.
