Facile Synthesis of Enzyme-Embedded Metal-Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent

In situimmobilization of enzyme into metal-organic frameworks (MOFs) is performed through a one-step and facile method.Candida antarcticalipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO3)(2).6H(2)O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB(R)ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB(R)ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.