Article
Cell & Tissue Engineering
Siyu Xia, Ming Wu, Xinhao Zhou, Xiu Zhang, Lina Ye, Kang Zhang, Yiyi Kang, Jun Liu, Yunci Zhang, Wang Wu, Dirong Dong, Hong Chen, Hui Li
Summary: Conditionally reprogrammed physiological endometrial epithelial cells provide a novel therapeutic strategy for IUA by repairing the injured endometrium and improving pregnancy rate.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Elena Garreta, Patricia Prado, Megan L. Stanifer, Vanessa Monteil, Andres Marco, Asier Ullate-Agote, Daniel Moya-Rull, Amaia Vilas-Zornoza, Carolina Tarantino, Juan Pablo Romero, Gustav Jonsson, Roger Oria, Alexandra Leopoldi, Astrid Hagelkruys, Maria Gallo, Federico Gonzalez, Pere Domingo-Pedrol, Aleix Gavalda, Carmen Hurtado del Pozo, Omar Hasan Ali, Pedro Ventura-Aguiar, Josep Maria Campistol, Felipe Prosper, Ali Mirazimi, Steeve Boulant, Josef M. Penninger, Nuria Montserrat
Summary: Diabetic individuals are more susceptible to severe COVID-19, and it appears that their kidney cells have altered metabolism, making them more prone to SARS-CoV-2 infection. The study also found that inhibiting glycolysis can reduce the infection.
Article
Urology & Nephrology
Yasuhiro Yoshimura, Yoshiharu Muto, Kohei Omachi, Jeffrey H. Miner, Benjamin D. Humphreys
Summary: This study reveals the regulatory mechanisms of HNF4A and HNF4G during human proximal tubule differentiation. The experimental strategy can be applied more broadly to investigate transcriptional regulation in human kidney development.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Review
Cell Biology
Danyal Daneshdoust, Mingjue Luo, Zaibo Li, Xiaokui Mo, Sahar Alothman, Bhaskar Kallakury, Richard Schlegel, Junran Zhang, Deliang Guo, Priscilla A. Furth, Xuefeng Liu, Jenny Li
Summary: Preclinical in vitro models, especially conditional reprogramming technology, are crucial in studying breast cancer and have the potential to improve drug discovery and personalized medicine in this field.
Review
Oncology
Faris Alkhilaiwi
Summary: Cancer is a devastating disease with a low percentage of patients benefiting from genomics-based precision oncology. Functional diagnostics and patient-derived cancer models are needed for precision cancer therapy. The combination of conditional cell reprogramming and robotic high-throughput screening has the potential to drive precision cancer therapy.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiaoxing Wu, Zhaoxia Li, Hongyu Zhang, Fang He, Min Qiao, Huaxiu Luo, Jing Zhang, Meng Zhang, Yukun Mao, William Wagstaff, Yongtao Zhang, Changchun Niu, Xia Zhao, Hao Wang, Linjuan Huang, Deyao Shi, Qing Liu, Na Ni, Kai Fu, Rex C. Haydon, Russell R. Reid, Hue H. Luu, Tong-Chuan He, Ziwei Wang, Houjie Liang, Bing-Qiang Zhang, Ning Wang
Summary: In this study, a conditionally immortalized mouse intestinal crypt (ciMIC) cell line was established using a piggyBac transposon-based SV40 T antigen expression system. The ciMICs maintained long-term proliferative activity under a two-dimensional niche factor-containing culture condition, retained the biological characteristics of intestinal epithelial stem cells, and could form intestinal organoids in three-dimensional culture. Additionally, ciMICs overexpressing oncogenic beta-catenin and/or KRAS exhibited high proliferative activity and developed intestinal adenoma-like pathological features in vivo, suggesting their potential as a valuable tool cell line for studying genetic and/or epigenetic mechanisms of intestinal tumorigenesis.
Article
Biochemical Research Methods
Jessica M. Vanslambrouck, Ker Sin Tan, Sophia Mah, Melissa H. Little
Summary: This article describes an improved method for generating kidney organoids with better proximal tubule maturity from human pluripotent stem cells. The enhanced organoids show increased expression of critical transporters and improved functionality, as assessed by substrate uptake and transport.
Article
Biochemistry & Molecular Biology
Jin-Yong Lee, Maki Tokumoto, Masahiko Satoh
Summary: Cadmium exerts its cytotoxicity through the inhibition of MEF2A activity and suppression of GLUT4 expression, leading to reduced cellular glucose concentration and ATP levels. This study provides a crucial breakthrough in understanding the mechanism of Cd toxicity in renal cells.
Article
Multidisciplinary Sciences
Waleed Rahmani, Hyunjae Chung, Sarthak Sinha, Maxwell P. Bui-Marinos, Rohit Arora, Arzina Jaffer, Jennifer A. Corcoran, Jeff Biernaskie, Justin Chun
Summary: COVID-19-associated acute kidney injury (COVID-AKI) is a common complication of SARS-CoV-2 infection in hospitalized patients. The susceptibility of human kidneys to direct SARS-CoV-2 infection and the modulation of the renin-angiotensin II signaling pathway by viral infection remain unclear. Losartan, an angiotensin II receptor blocker being tested in COVID-19 patients, has shown potential protective effects against SARS-CoV-2 infection.
Review
Urology & Nephrology
Julie Bejoy, Eddie S. Qian, Lauren E. Woodard
Summary: AKI affects millions of people worldwide each year, but regenerative therapies are still not available. Human kidney tissue culture systems and human pluripotent stem cells offer new approaches to studying AKI mechanisms and developing new therapeutics.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2022)
Article
Pharmacology & Pharmacy
Yi Yang, Milos Mihajlovic, Floris Valentijn, Tri Q. Nguyen, Roel Goldschmeding, Rosalinde Masereeuw
Summary: Accumulating evidence suggests that senescence of kidney tubule epithelial cells leads to fibrosis. In this study, the researchers investigated the use of a specific cell line, ciPTEC-OAT1, as an in vitro model to study kidney senescence and the response to senolytics. They found that culturing ciPTEC-OAT1 at 37 degrees C induced a senescence phenotype characterized by increased expression of cell cycle arrest and anti-apoptosis markers, SASP factors, and responsiveness to senolytics treatment.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biology
Ramin Banan Sadeghian, Ryohei Ueno, Yuji Takata, Akihiko Kawakami, Cheng Ma, Toshikazu Araoka, Minoru Takasato, Ryuji Yokokawa
Summary: Recently, microphysiological systems have been used to model the renal proximal tubule. However, there is a lack of research on refining the functions of the proximal tubule epithelial layer. In this study, pseudo proximal tubule cells from human-induced pluripotent stem cell-derived kidney organoids were combined and cultured with immortalized proximal tubule cells. The cocultured tissue showed improved levels of certain transporters, extracellular matrix proteins, glucose transport, and P-glycoprotein activity. The morphological characteristics and performance of the cocultured tissue, when exposed to human umbilical vein endothelial cells, were also analyzed and quantified.
COMMUNICATIONS BIOLOGY
(2023)
Article
Cell Biology
Hanna Schmidt, Lara Guthjahr, Alexander Sauter, Fabian Zech, Rayhane Nchioua, Steffen Stenger, Manfred Frick, Frank Kirchhoff, Paul Dietl, Oliver H. Wittekindt
Summary: This study evaluated the use of serially passaged crNECs as a model to study SARS-CoV-2 infection and its effects on ion and water transport. The results demonstrated that the cells maintained their major differentiation characteristics and physiological properties throughout all passages, but there were gradual decreases in the frequency of ciliated cells, ion channel activity, and expression of water channel proteins.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yapei Yuan, Huan Chen, Sihua Ou, Baomei Cai, Ruifang Zhang, Yue Qin, Mengjie Pan, Shangtao Cao, Duanqing Pei, Fan Fan Hou
Summary: This study successfully generated mitochondria-rich kidney organoids via partial reprogramming of human urine cells, which recapitulated the structural and functional characteristics of the kidneys, providing a promising model to study mitochondria-related kidney physiology and disease in a personalized manner.
CELL AND BIOSCIENCE
(2022)
Article
Oncology
Jennifer Eymael, Martijn van den Broek, Laura Miesen, Valerie Villacorta Monge, Bartholomeus T. van den Berge, Fieke Mooren, Vicky Luna Velez, Jelmer Dijkstra, Meyke Hermsen, Peter Bandi, Michiel Vermeulen, Saskia de Wildt, Brigith Willemsen, Sandrine Florquin, Roy Wetzels, Eric Steenbergen, Rafael Kramann, Marcus Moeller, Michiel F. Schreuder, Jack Fm Wetzels, Johan van der Vlag, Jitske Jansen, Bart Smeets
Summary: Scattered tubular cells (STCs) in the proximal tubule increase in number after acute kidney injury. STCs are preferentially located within the inner bends of the tubule and their number increases with age. STCs represent a transient state of dedifferentiated proximal tubule epithelial cells (PTECs) showing metabolic shift towards glycolysis, facilitating cellular survival after kidney injury.
JOURNAL OF PATHOLOGY
(2023)
Article
Biochemical Research Methods
Siyu Xia, Jing Feng, Lijun Lei, Jun Hu, Linjian Xia, Jun Wang, Yu Xiang, Lingjun Liu, Shan Zhong, Leng Han, Chunjiang He
BRIEFINGS IN BIOINFORMATICS
(2017)
Article
Biochemistry & Molecular Biology
Siyu Xia, Jing Feng, Ke Chen, Yanbing Ma, Jing Gong, Fangfang Cai, Yuxuan Jin, Yang Gao, Linjian Xia, Hong Chang, Lei Wei, Leng Han, Chunjiang He
NUCLEIC ACIDS RESEARCH
(2018)
Article
Cell & Tissue Engineering
Siyu Xia, Jun Liu, Yan Yang, Ming Wu, Lina Ye, Si Chen, Tao Zhang, Zhihong Zeng, Kang Zhang, Kaihong Cai, Xiang Long, Wenbin Gao, Shisong Fang, Hui Li
STEM CELLS INTERNATIONAL
(2020)
Article
Cell & Tissue Engineering
Siyu Xia, Ming Wu, Xinhao Zhou, Xiu Zhang, Lina Ye, Kang Zhang, Yiyi Kang, Jun Liu, Yunci Zhang, Wang Wu, Dirong Dong, Hong Chen, Hui Li
Summary: Conditionally reprogrammed physiological endometrial epithelial cells provide a novel therapeutic strategy for IUA by repairing the injured endometrium and improving pregnancy rate.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Oncology
Siyu Xia, Wei Zhang, Jing Yang, Shuang Wang, Chuanbin Yang, Jigang Wang
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Cell Biology
Chuanbin Yang, Siyu Xia, Wei Zhang, Han-Ming Shen, Jigang Wang
Summary: Using single-cell RNA sequencing, this study found that the transcription patterns of Atg genes in different cell types of rat liver, brain, and kidney are highly heterogeneous. Caloric restriction can reverse the changes of these genes during aging.
Article
Medicine, Research & Experimental
Wei Zhang, Siyu Xia, Xiaoru Zhong, Guoyong Gao, Jing Yang, Shuang Wang, Min Cao, Zhen Liang, Chuanbin Yang, Jigang Wang
Summary: This study utilized single-cell RNA sequencing to investigate the molecular mechanisms and pathways of 2,2',4,4'-tetrabromodiphenyl ether (BDE47)-induced testicular toxicity in mice. The results revealed hormone homeostasis disruption and downregulation of previously unappreciated pathways, providing new insight into the impairment of spermatogenesis caused by BDE47.
PRECISION CLINICAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Wei Zhang, Siyu Xia, Wei Xiao, Yali Song, Li Tang, Min Cao, Jing Yang, Shuang Wang, Zhijie Li, Chengchao Xu, Jianqiao Liu, Shanchao Zhao, Chuanbin Yang, Jigang Wang
Summary: This study focuses on the reproductive issues of advanced paternal age and analyzes the molecular and cellular changes associated with aging in the testes of mice. The study discovers various aging-related biological processes and identifies an increased subtype of aging-specific macrophages that contribute to a proinflammatory environment during testicular aging.
JOURNAL OF ADVANCED RESEARCH
(2023)