Article
Biochemistry & Molecular Biology
Hyocheol Bae, Sunwoo Park, Jiyeon Ham, Jisoo Song, Taeyeon Hong, Jin-Hee Choi, Gwonhwa Song, Whasun Lim
Summary: Beta-sitosterol, a plant-derived phytosterol, has been found to exhibit anticancer effects against ovarian cancer by inducing apoptosis, suppressing proliferation, inhibiting migration, and showing synergistic effects with standard anticancer drugs.
Review
Biochemistry & Molecular Biology
Dong-Oh Moon
Summary: This review provides a comprehensive analysis of the multifaceted role of calcium in apoptosis regulation, focusing on its associated signaling pathways and molecular mechanisms. It explores the impact of calcium on apoptosis through its effects on various cellular compartments and highlights its connection to ER stress. Additionally, it discusses the interplay between calcium and proteins such as calpains, calmodulin, and Bcl-2 family members, and its role in regulating caspase activation and pro-apoptotic factor release. Understanding the complex relationship between calcium and apoptosis is crucial for identifying potential treatment options for diseases associated with imbalanced cell death.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Alberto Danese, Sara Leo, Alessandro Rimessi, Mariusz R. Wieckowski, Francesco Fiorica, Carlotta Giorgi, Paolo Pinton
Summary: Calcium ions and their regulatory system play crucial roles in cell life and death decisions, with disruptions in this system potentially leading to conditions such as cancer.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Taeyeon Hong, Jiyeon Ham, Gwonhwa Song, Whasun Lim
Summary: Alpinumisoflavone inhibits ovarian cancer cell growth by regulating cell proliferation, apoptosis, and mitochondrial function.
Article
Biochemistry & Molecular Biology
Francisco Sadras, Teneale A. Stewart, Melanie Robitaille, Amelia A. Peters, Priyakshi Kalita-de Croft, Patsy S. Soon, Jodi M. Saunus, Sunil R. Lakhani, Sarah J. Roberts-Thomson, Gregory R. Monteith
Summary: The role of calcium signalling pathways in inducing CAF phenotype has not been fully explored. A CAF model was generated by stimulating normal human mammary fibroblast cells with TGF beta 1, leading to altered calcium influx pathways. Upregulation of VGCCs Ca(V)1.2 and Ca(V)3.2 was observed in both the model and patient-derived breast CAFs, and inhibiting these channels impaired CAF activation. Targeting calcium signalling in breast CAFs could have a therapeutic potential.
Article
Biochemistry & Molecular Biology
Taewoo Kim, Seong-Gyu Ko
Summary: JI017 is a potent anti-cancer drug for ovarian cancer treatment, mediating endoplasmic reticulum stress and apoptosis through the Nox4-PERK-CHOP signaling pathway. Combination treatment with radiation can overcome radioresistance in ovarian cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Peiran Zhang, Xianchun Yan, Xiaoyan Zhang, Yuan Liu, Xingxing Feng, Ziyan Yang, Jiayulin Zhang, Xinyuan Xu, Qijun Zheng, Liang Liang, Hua Han
Summary: TMEM215 is induced by blood flow-derived shear stress through downregulating EZH2. TMEM215 protects endothelial cells (ECs) from BIK-triggered mitochondrial apoptosis mediated by calcium influx through mitochondria-associated ER membranes during vessel pruning. Loss of Tmem215 in ECs promotes vascular regression and inhibits tumor growth, but attenuates lung metastasis.
CIRCULATION RESEARCH
(2023)
Article
Cell Biology
Hyocheol Bae, Jin-Young Lee, Jisoo Song, Gwonhwa Song, Whasun Lim
Summary: Osthole, a natural coumarin, shows promising anticancer effects against ovarian cancer by targeting the endoplasmic reticulum-mitochondrial axis and phosphatidylinositol 3-kinase/mitogen-activated protein kinase signaling pathway. Its pharmacological potential is demonstrated in a three-dimensional tumor-formation model using the zebrafish xenograft assay, indicating strong potential as a pharmacological agent for targeting ovarian cancer.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biology
Luana Naia, Catarina M. Pinho, Giacomo Dentoni, Jianping Liu, Nuno Santos Leal, Duarte M. S. Ferreira, Bernadette Schreiner, Riccardo Filadi, Ligia Fao, Niamh M. C. Connolly, Pontus Forsell, Gunnar Nordvall, Makoto Shimozawa, Elisa Greotti, Emy Basso, Pierre Theurey, Anna Gioran, Alvin Joselin, Marie Arsenian-Henriksson, Per Nilsson, A. Cristina Rego, Jorge L. Ruas, David Park, Daniele Bano, Paola Pizzo, Jochen H. M. Prehn, Maria Ankarcrona
Summary: This study established a large-scale screening platform for mitochondrial-based modulators and identified luteolin as a compound with potential therapeutic validity for various human diseases by increasing mitochondrial activity through enhancing interactions between mitochondria and endoplasmic reticulum. The findings suggest a novel mechanism for luteolin's modulation of mitochondrial function in neuronal models and potential therapeutic applications.
Article
Biochemistry & Molecular Biology
Baojun Sun, Hitomi Maruta, Yun Ma, Hiromi Yamashita
Summary: Taurine is an abundant free amino acid in mammalian tissues that plays a role in skeletal muscle function and exercise capacity. This study investigated the mechanism of taurine function in skeletal muscles by examining the effects of short-term administration of taurine on rats and L6 cells. The results suggest that taurine modulates skeletal muscle function by activating AMP-activated protein kinase through the calcium signaling pathway, leading to the expression of genes and proteins associated with mitochondrial and respiratory metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yixuan Zhang, Jianzhuang Wu, Yao Fu, Ranran Yu, Haochen Su, Qisi Zheng, Hao Wu, Siqi Zhou, Kun Wang, Jing Zhao, Shanshan Shen, Guifang Xu, Lei Wang, Chao Yan, Xiaoping Zou, Ying Lv, Shu Zhang
Summary: This study identified Hesperadin as a potent anti-cancer compound against pancreatic cancer (PC) by inhibiting cell growth and inducing apoptotic cell death. Mechanistic studies revealed that Hesperadin increased GADD45A expression via ATF4, leading to apoptosis. Immunohistochemical staining demonstrated the correlation between ATF4 and GADD45A expression. PC xenograft studies confirmed the inhibitory effect of Hesperadin on PC cell growth in vivo.
Article
Multidisciplinary Sciences
Dorian Ziegler, David Vindrieux, Delphine Goehrig, Sara Jaber, Guillaume Collin, Audrey Griveau, Clotilde Wiel, Nadia Bendridi, Sophia Djebali, Valerio Farfariello, Natacha Prevarskaya, Lea Payen, Jacqueline Marvel, Sebastien Aubert, Jean-Michel Flaman, Jennifer Rieusset, Nadine Martin, David Bernard
Summary: The lack of ITPR2 in mice leads to improved aging, which is associated with a decreased number of contacts between mitochondria and endoplasmatic reticulum.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Jing Li, Fangzheng Qi, Huishan Su, Chuanshan Zhang, Qing Zhang, Ying Chen, Ping Chen, Linjia Su, Yanan Chen, Yuqi Yang, Zhesheng Chen, Sihe Zhang
Summary: GRP75-faciliated MAM formation is enriched in cisplatin-resistant ovarian cancer cells. Diminishing MAM integrity by GRP75-deficiency reduces ER-to-mitochondrial Ca2+ transfer, accelerates mitochondrial dysfunction and ROS accumulation, and enhances cisplatin-triggered apoptotic cell death.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Peripheral Vascular Disease
Linqiang Ma, Shumin Yang, Yi Yang, Xiangjun Chen, Jun Yang, Yifan He, Qingfeng Cheng, Junlong Li, Bing Kang, Siying Tang, Aipin Zhang, Xiaoyu Shu, Xiaoyu Li, Hang Shen, Ying Jing, Ying Song, Qifu Li, Jinbo Hu
Summary: This study investigates the role of wolframin in aldosterone hypersecretion and finds that it is involved in calcium transportation and mitochondrial function, which regulate aldosterone secretion.
Article
Cell Biology
Laura Lopez-Molina, Joaquin Fernandez-Irigoyen, Carmen Cifuentes-Diaz, Jordi Alberch, Jean-Antoine Girault, Enrique Santamaria, Silvia Gines, Albert Giralt
Summary: This study explores the function of Pyk2 in mitochondria-associated membranes (MAMs) and demonstrates its role in regulating ER-mitochondria calcium efflux, affecting mitochondrial function and leading to calcium homeostasis imbalance.
Article
Biochemistry & Molecular Biology
Lihyun Kim, Taeyeon Hong, Jiyeon Ham, Whasun Lim
Summary: The present study aimed to investigate the inhibitory effects of Agarum clathratum on the viability of epithelial ovarian cancer cells. The results showed that Agarum clathratum extract suppressed the viability of the cancer cells and affected the intracellular Ca2+ levels and mitochondrial membrane potential. Furthermore, the extract inhibited the PI3K/AKT and MAPK pathways.
MOLECULAR & CELLULAR TOXICOLOGY
(2023)
Article
Engineering, Environmental
Nayoung Min, Hahyun Park, Taeyeon Hong, Garam An, Gwonhwa Song, Whasun Lim
Summary: This study evaluated the developmental toxicity of the herbicide prometryn in zebrafish embryos, revealing that it shortened body length, induced organ development defects, reactive oxygen species accumulation, and cell death. It was also found to interfere with mitochondrial function and energy metabolism.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Garam An, Miji Kim, Junho Park, Hahyun Park, Taeyeon Hong, Whasun Lim, Gwonhwa Song
Summary: Chloroxylenol is a widely used anti-microbial compound, and recent studies have emphasized the need to assess its potential for ecotoxicity due to increased usage during the coronavirus pandemic and ban on other antimicrobial ingredients. Although the detrimental effect of chloroxylenol on zebrafish viability has been reported, the mechanisms underlying its toxicity are not well understood. This study used the zebrafish model to investigate the responses to chloroxylenol and predict its toxicity to human health and ecology. The findings suggest the risks of chloroxylenol exposure and highlight the importance of further ecotoxicological studies.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Developmental Biology
Miji Kim, Junho Park, Garam An, Whasun Lim, Gwonhwa Song
Summary: In this study, it was found that pendimethalin exposure disrupted the Ca2+ homeostasis, mitochondrial membrane potential, mitogen-activated protein kinase signaling pathway, and implantation-related genes in porcine trophectoderm and uterine luminal epithelial cells. Pendimethalin is widely used as a herbicide in agriculture, but its toxicity mechanism during the pre-implantation stage has not been thoroughly investigated. This study provides important insights into the toxic effects of pendimethalin on porcine cells, highlighting the potential risks it poses to the implantation process in pigs.
Article
Obstetrics & Gynecology
Daryeon Son, Hahyun Park, Garam An, Sunwoo Park, Dong Won Hwang, Soo Jin Park, Hee Seung Kim, Whasun Lim, Seungkwon You, Gwonhwa Song
Summary: Endometriosis is an inflammatory disease characterized by the growth of endometrial-like tissues outside the uterus, and progesterone insufficiency contributes to its progression and systemic inflammation. Patients with endometriosis often experience painful symptoms and infertility, significantly affecting their quality of life.
REPRODUCTIVE SCIENCES
(2023)
Article
Environmental Sciences
Junho Park, Taeyeon Hong, Garam An, Hahyun Park, Gwonhwa Song, Whasun Lim
Summary: Various types of fungicides, including triadimenol, are used to protect crops from fungal diseases. However, the developmental toxicity of triadimenol on zebrafish is not well understood. In this study, we used the zebrafish animal model to investigate the effects of triadimenol. Triadimenol caused morphological abnormalities, increased reactive oxygen species production, inflammation-related gene expression, and apoptosis in zebrafish, especially in the heart region. It also disrupted the PI3K and MAPK signaling pathways, leading to malformation of the heart and vascular structures. These findings provide insights into the mechanisms underlying triadimenol toxicity in zebrafish.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Reproductive Biology
Wonhyoung Park, Junho Park, Sunwoo Park, Whasun Lim, Gwonhwa Song
Summary: This study found that picolinafen has a significant impact on the viability of porcine trophectoderm and luminal epithelial cells involved in the implantation process during early pregnancy. It increased apoptosis, disrupted mitochondrial function, and caused intracellular ROS accumulation, which led to a reduction in calcium levels. Furthermore, it inhibited the migration of porcine trophectoderm cells. These effects were mediated by the activation of the MAPK and PI3K signaling pathways.
Article
Biochemistry & Molecular Biology
Junho Park, Garam An, Jeankyoung You, Hahyun Park, Taeyeon Hong, Gwonhwa Song, Whasun Lim
Summary: This study used zebrafish models to verify the developmental toxicity of dimethenamid. Dimethenamid not only caused morphological abnormalities and reduced viability in zebrafish larvae, but also promoted ROS production, inflammation responses, and uncontrolled apoptosis. Moreover, dimethenamid induced toxic effects on the cardiovascular system, liver, and pancreas.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Garam An, Junho Park, Jeankyoung You, Hahyun Park, Taeyeon Hong, Whasun Lim, Gwonhwa Song
Summary: This study assessed the environmental hazards and toxic mechanisms of flufenacet using a zebrafish model. The results showed that flufenacet had developmental toxicity in zebrafish and adversely affected the development of specific organs. The study also verified the intracellular actions of flufenacet in zebrafish, including apoptosis, cell cycle modulation, and Mapk and Akt signaling pathway.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Miji Kim, Taeyeon Hong, Garam An, Whasun Lim, Gwonhwa Song
Summary: This study demonstrated the developmental toxicity and mechanism of action of the herbicide benfluralin using zebrafish as a model. Benfluralin caused morphological and physiological alterations, increased reactive oxygen species (ROS) levels, induced heart and vascular structure malformations, and inhibited liver development. It also affected normal growth by abnormal expression of cell cycle regulatory genes and increased oxidative stress, inflammation, and apoptosis.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hakyoung You, Garam An, Hojun Lee, Whasun Lim, Gwonhwa Song
Summary: In this study, we investigated the toxic effects of bifenox on bovine mammary glands using MAC-T cells. Our findings showed that bifenox inhibited cell proliferation, disrupted the cell cycle, and caused disturbances in calcium homeostasis and mitochondrial membrane potential. We also observed hyperactivation of PI3K/AKT and MAPK signaling cascades in response to bifenox treatment.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Eunho Sung, Junho Park, Hojun Lee, Gwonhwa Song, Whasun Lim
Summary: The toxic effects of bifenthrin on bovine mammary epithelial cells have been investigated, revealing that it inhibits cell proliferation, induces apoptosis, disrupts mitochondrial function and calcium ion homeostasis, alters inflammatory gene expression, and downregulates casein-related genes.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Environmental Sciences
Hojun Lee, Garam An, Junho Park, Whasun Lim, Gwonhwa Song
Summary: This study demonstrates the toxic effects of molinate on zebrafish larvae, including reduced viability and successful hatching, and the induction of apoptosis, inflammation, and endoplasmic reticulum stress response. Additionally, molinate exposure leads to abnormal cardiovascular, neuronal, and liver development in zebrafish.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Nayoung Min, Hahyun Park, Taeyeon Hong, Jisoo Song, Gwonhwa Song, Whasun Lim
Summary: This study investigated the embryonic developmental toxicity of the herbicide terbutryn in zebrafish. The results showed that terbutryn exposure led to increased mortality, reduced body and eye size, and yolk sac edema in zebrafish. Fluorescence microscopy revealed the effects of terbutryn on blood vessel, motor neuron, and liver development, and apoptotic cell death was evaluated using acridine orange staining.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Garam An, Junho Park, Whasun Lim, Gwonhwa Song
Summary: In this study, we investigated the hepatotoxicity and mechanism of action of pyridaben using zebrafish and ZFL cells. Pyridaben caused liver size reduction and apoptosis, and these effects were associated with uncontrolled intracellular calcium flow and downregulation of the PI3K/Akt signaling cascade.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
