Article
Pharmacology & Pharmacy
Qinghai Zhu, Yuting Tang, Tian Zhou, Li Yang, Gao Zhang, Ying Meng, Huixin Zhang, Jun Gao, Chenxing Wang, Yu-Xiong Su, Jinhai Ye
Summary: This study found that exosomes derived from pre-differentiated AB-BMSCs carrying specific miRNAs have a positive effect on bone regeneration. The 7-day pre-differentiation of exosomes effectively promoted BMSCs differentiation and activated the PI3K/Akt signaling pathway through differential expression of miRNAs. Gene modification of exosomes provides great potential as a bone regeneration strategy.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Oncology
Yuxiao Chang, Ziran Huang, Fengyi Hou, Yuejiao Liu, Likun Wang, Zhen Wang, Yifan Sun, Zhiyuan Pan, Yafang Tan, Lei Ding, Hong Gao, Ruifu Yang, Yujing Bi
Summary: This study revealed an important relationship between Parvimonas micra (P. micra) and colorectal cancer (CRC) through cell experiments and mouse models. Transcriptomics and microRNA sequencing showed that P. micra promoted CRC development through the activation of the miR-218-5p/Ras/ERK/c-Fos pathway.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Cell Biology
Yongkun Wei, Huiling Ma, Haiqing Zhou, Hanrong Yin, Jie Yang, Yongcai Song, Binhui Yang
Summary: Research indicates that overexpression of miR-424-5p in BMSCs-derived exosomes can regulate osteogenic differentiation of osteoblasts through the WIF1/Wnt/beta-catenin pathway, suggesting miR-424-5p as a potential new biomarker for the treatment of osteoporosis.
Article
Biotechnology & Applied Microbiology
Yingshuai Wang, Tao Yu, Feihu Hu
Summary: This study explores the application of miRNAs and exosomes in peripheral nerve regeneration and finds that a hypocapnia environment can enhance exosome function. By delivering miR-218 through engineered exosomes, sciatic nerve regeneration can be facilitated, and hypocapnia stimuli-responsive exosomes show better results.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Qingju Li, Yinchuan Xu, Kaiqi Lv, Yingchao Wang, Zhiwei Zhong, Changchen Xiao, Keyang Zhu, Cheng Ni, Kan Wang, Minjian Kong, Xuebiao Li, Youqi Fan, Fengjiang Zhang, Qi Chen, Yi Li, Qian Li, Chengjia Liu, Jinyun Zhu, Shuhan Zhong, Jingyi Wang, Yongjian Chen, Jing Zhao, Dan Zhu, Rongrong Wu, Jinghai Chen, Wei Zhu, Hong Yu, Reza Ardehali, Jianyi (Jay) Zhang, Jian'an Wang, Xinyang Hu
Summary: This study highlights the key role of sEV miR-486-5p in promoting cardiac angiogenesis. miR-486-5p acts on the fibroblastic MMP19-VEGFA signaling pathway to promote cardiac recovery. Delivery of miR-486-5p-engineered sEVs safely enhanced angiogenesis and cardiac function in a nonhuman primate (NHP) MI model, potentially promoting cardiac repair.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Engineering, Biomedical
Kai Shen, Ao Duan, Jiangqi Cheng, Tao Yuan, Jinchun Zhou, Huanghe Song, Zhefeng Chen, Bin Wan, Jiuxiang Liu, Xiao Zhang, Yi Zhang, Rui Xie, Feng Liu, Weimin Fan, Qiang Zuo
Summary: Tissue engineering and exosomes have promising prospects for cartilage regeneration. Hypoxia preconditioned exosomes can enhance the function of articular chondrocytes and promote cartilage repair via the miR-205-5p/PTEN/AKT pathway.
ACTA BIOMATERIALIA
(2022)
Article
Biotechnology & Applied Microbiology
Xian Xian, Li-Li Cai, Yang Li, Ran-Chao Wang, Yu-Hao Xu, Ya-Jie Chen, Yu-Hang Xie, Xiao-Lan Zhu, Yue-Feng Li
Summary: This study found significantly higher expression levels of miR-9-5p in serum exosomes from patients with depression. Using experimental models, the researchers observed that miR-9-5p caused polarization of microglia and excessive release of inflammatory factors, exacerbating neuronal damage. The study also identified SOCS2 as a target of miR-9-5p. Overexpression of miR-9-5p suppressed SOCS2 expression and activated the JAK/STAT3 pathway. The findings suggest that the expression and secretion of miR-9-5p may serve as novel therapeutic targets for depression.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Materials Science, Biomaterials
Xin Luo, Chunxiu Meng, Yujue Zhang, Qicui Du, Caiyao Hou, Huifen Qiang, Kun Liu, Zhaoyong Lv, Jun Li, Fengzhen Liu
Summary: Bone immune responses mediated by macrophages are crucial for bone abnormalities. While M2 macrophages promote osteogenesis, previous studies indicated that M1 macrophages play a significant role in early bone healing. However, the involvement of M1 macrophages in bone immune response remains unclear. This study focuses on the role of M1 macrophages and their miR-21a-5p exosomes in bone healing, demonstrating their potential to enhance bone regeneration and osteoblast differentiation by targeting GATA2.
REGENERATIVE BIOMATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Shaowei Pan, Zhaowei Yin, Chen Shi, Haonan Xiu, Guanfu Wu, Yongyuan Heng, Zhangyu Zhu, Jing Zhang, Jianchao Gui, Ziyi Yu, Bin Liang
Summary: The study investigated the enhancement of osteogenic and angiogenic potential of Exosomes through overexpression of exosomal miRNA (microRNA) and the long-term effects of Exos loaded in HMPs (Hydrogel microparticles) on new bone formation. The research demonstrated that miR-29a overexpression inhibited HDAC4 and upregulated RUNX2 and VEGF, enhancing the osteogenic and angiogenic capabilities. The synthesized HMP@Exo system showed good injectability, compatibility, and cell adhesion properties, with sustained release of Agomir-29a-Exos enhancing cell proliferation, migration, osteogenesis, and angiogenesis. Overall, the injectable HMP@Exo system provides an innovative approach for coupling osteogenesis and angiogenesis, promoting fracture healing.
Article
Gastroenterology & Hepatology
Jifei Wang, Wangjie Jiang, Shuochen Liu, Kuangheng Shi, Yaodong Zhang, Yananlan Chen, Jijun Shan, Yuming Wang, Xiao Xu, Changxian Li, Xiangcheng Li
Summary: The study found that miR-182-5p can be taken up by exosomes secreted from cholangiocarcinoma, promoting vascular endothelial cell proliferation, migration, and angiogenesis. Additionally, miR-182-5p also activates the PI3K/AKT/mTOR signaling pathway, leading to drug resistance in cholangiocarcinoma.
LIVER INTERNATIONAL
(2023)
Article
Oncology
Yingying Qi, Lina Guo, Yanchao Liu, Tonghang Zhao, Xianwen Liu, Yang Zhang
Summary: Sevoflurane inhibited proliferation, migration, and invasion while enhancing apoptosis in glioma cells. MiR-218-5p played a critical role in this process. The study demonstrated that sevoflurane suppressed glioma cell growth and invasion by modulating the miR-218-5p/DEK/beta-catenin axis.
CANCER MANAGEMENT AND RESEARCH
(2021)
Article
Dermatology
Fang Liu, Si Liu, Xiaohua Luo, Zirui Fan, Shaobin Huang, Fangqi Deng, Huanliang Liu, Ge Shi
Summary: The study demonstrates that exosomes derived from DSCCs have an anti-ageing effect on DPCs, promoting their proliferation, viability, and migration while inhibiting apoptosis. The exosomes also enhance hair follicle induction ability and activate relevant signaling pathways, potentially serving as a new strategy for preventing senile alopecia.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Medicine, Research & Experimental
Hongtao He, Muliang Ding, Tao Li, Wenzhi Zhao, Lu Zhang, Peng Yin, Wei Zhang
Summary: This study discovers that NORAD, a non-coding RNA highly expressed in osteosarcoma cells, can be delivered to osteosarcoma cells through extracellular vesicles (EVs) released by bone mesenchymal stem cells (BMSCs), thereby promoting proliferation and invasion of osteosarcoma. NORAD is found to interact with miR-30c-5p and KLF10, and regulates the miR-30c-5p/KLF10 axis to influence the development and metastasis of osteosarcoma.
LABORATORY INVESTIGATION
(2022)
Article
Cardiac & Cardiovascular Systems
Guo-wei Tu, Jie-fei Ma, Jia-kun Li, Ying Su, Jing-chao Luo, Guang-wei Hao, Ming-hao Luo, Yi-rui Cao, Yi Zhang, Zhe Luo
Summary: The miR-885-5p/HMBOX1 axis plays a significant role in septic myocardial depression. Sepsis-exos promote pyroptosis in AC16 cells through miR-885-5p, and HMBOX1 reverses this effect in an NF-kappa B-dependent manner.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Engineering, Biomedical
Yahong Zhao, Jina Liu, Sha Liu, Panpan Yang, Yunyun Liang, Jinyu Ma, Susu Mao, Cheng Sun, Yumin Yang
Summary: In this study, the protein expression profiles in injured sciatic nerves were analyzed using proteomics. It was found that chitosan oligosaccharides (COS) up-regulate a group of proteins related to exosome packaging and transport, indicating the involvement of exosomes in COS-induced peripheral nerve regeneration. Exosomes derived from COS-treated fibroblasts promote axon extension and regeneration, and the key factor in this process is TFAP2C.
BIOACTIVE MATERIALS
(2023)
Review
Biotechnology & Applied Microbiology
Mira Chingale, Dashuai Zhu, Ke Cheng, Ke Huang
Summary: The combination of bioengineering and cardiac regenerative medicine provides innovative solutions to challenges such as lack of adult cardiac stem cells and difficulty in therapeutic delivery to the injured heart. Advanced technologies like cell reprogramming, gene editing, and protein factors are being utilized to overcome limitations and improve outcomes in cardiac regenerative medicine.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Environmental
Junlang Li, Yongbo Lv, Dashuai Zhu, Xuan Mei, Ke Huang, Xianyun Wang, Zhenhua Li, Sichen Zhang, Shiqi Hu, Kristen D. Popowski, Ke Cheng, Juan Wang
Summary: A comparison between intramyocardial (IM) injection and intrapericardial cavity (IPC) injection of adult stem cells in hydrogel showed that IPC injection led to better retention and therapeutic benefits, resulting in significant improvement in cardiac function. Furthermore, IPC delivery demonstrated better cellular proliferation, less apoptosis, and improved vascular regeneration in the myocardium. Additionally, IPC injections led to higher rates of MSC-exosome absorption by heart cells, indicating more extensive paracrine activity and showing promise as a solution for the low retention issue of MSCs in the infarcted heart.
CHEMICAL ENGINEERING JOURNAL
(2022)
Review
Materials Science, Multidisciplinary
Mira Chingale, Ke Cheng, Ke Huang
Summary: Cardiovascular diseases are a leading cause of death globally. Heart transplantation is a common treatment for patients with end stage heart failure. However, due to the limited availability of donors, the success of heart transplantation depends on various factors and can be affected by immunological issues. 3D bioprinting is an emerging technique for generating functional cardiac tissues in vitro, which can be used for drug screening and cardiac regenerative therapy.
FRONTIERS IN MATERIALS
(2022)
Review
Materials Science, Biomaterials
Yaching Hsu, Ke Huang, Ke Cheng
Summary: Heart failure is a leading cause of hospital admissions worldwide, with patients classified based on left ventricular ejection fraction changes. Current treatments can prevent one type of heart failure from progressing, but few have been shown to improve survival in another type.
Article
Cardiac & Cardiovascular Systems
George Cheng, Dashuai Zhu, Ke Huang, Thomas G. Caranasos
Summary: Coronary heart disease is the leading cause of death in the United States. Injecting a hydrogel containing exosomes has shown potential in reducing heart chamber size and preserving wall thickness.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Yihan Liu, Rahul Menon, Arjun Putcha, Ke Huang, Leonardo Bonilla, Rohan Vora, Junye Li, Lin Zhang, Yihang Wang, Lauren Fletcher, Anna Lassiter, Chuqi Huang, John B. Buse, Ke Cheng, Wubin Bai
Summary: This study introduces a wireless wearable deep-tissue sensing patch that utilizes biocompatible microneedle waveguides to monitor the oxygen saturation in muscular tissue in real-time. The patch provides multiple physiological measurements and ensures durable and safe operation at the skin interface.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Engineering, Biomedical
Zhenzhen Wang, Kristen D. Popowski, Dashuai Zhu, Blanca Lopez de Juan Abad, Xianyun Wang, Mengrui Liu, Halle Lutz, Nicole De Naeyer, C. Todd DeMarco, Thomas N. Denny, Phuong-Uyen C. Dinh, Zhenhua Li, Ke Cheng
Summary: Researchers have developed an inhalable and room-temperature-stable virus-like particle COVID-19 vaccine that induces systemic and mucosal immune responses in small animals. The vaccine, consisting of a recombinant SARS-CoV-2 receptor-binding domain conjugated to lung-derived exosomes, enhanced the retention of the receptor-binding domain in the respiratory airway and lungs, and cleared SARS-CoV-2 pseudovirus in mice. In hamsters, the vaccine attenuated severe pneumonia and reduced inflammatory infiltrates after a challenge with live SARS-CoV-2.
NATURE BIOMEDICAL ENGINEERING
(2022)
Article
Cardiac & Cardiovascular Systems
Dashuai Zhu, Shuo Liu, Ke Huang, Zhenzhen Wang, Shiqi Hu, Junlang Li, Zhenhua Li, Ke Cheng
Summary: This study reveals that the pericardial drainage pathway promotes immune activation in the mediastinal lymph node following myocardial infarction, and MSC exosomes induce regulatory T cell differentiation through the Foxo3 pathway to promote cardiac repair.
CIRCULATION RESEARCH
(2022)
Editorial Material
Engineering, Biomedical
Ke Huang, Ke Cheng
Summary: Inflamed heart tissue can be repaired after a heart attack by using a degradable matrix that can be infused intravascularly and binds to leaky blood vessels.
NATURE BIOMEDICAL ENGINEERING
(2023)
Article
Cardiac & Cardiovascular Systems
Dashuai Zhu, Shuo Liu, Ke Huang, Junlang Li, Xuan Mei, Zhenhua Li, Ke Cheng
Summary: The aim of this study was to investigate the mechanisms of extracellular vesicle (EV) therapy for improving cardiac remodeling and develop a promising treatment for myocardial fibrosis. It was found that Tcf21 could be a potential target to improve cardiac remodeling, and lncRNA-TARID, which was enriched in EVs, could up-regulate Tcf21 expression. Treatment with lncRNA-TARID-laden lipid nanoparticles improved cardiac function and histology in mouse and porcine models of myocardial infarction. This study identified novel mechanisms underlying EV therapy for cardiac remodeling and proposed a lncRNA therapy for cardiac fibrosis.
EUROPEAN HEART JOURNAL
(2023)
Article
Chemistry, Physical
Xuan Mei, Junlang Li, Zhenzhen Wang, Dashuai Zhu, Ke Huang, Shiqi Hu, Kristen D. Popowski, Ke Cheng
Summary: Researchers developed an inhalable bioadhesive hydrogel called SHIELD, which forms a dense hydrogel layer in the airway to enhance barrier properties against SARS-CoV-2 infection. The method was shown to effectively protect against SARS-CoV-2 virus variants in mice and non-human primates.
Article
Multidisciplinary Sciences
Zhenzhen Wang, Zhenhua Li, Weiwei Shi, Dashuai Zhu, Shiqi Hu, Phuong-Uyen C. Dinh, Ke Cheng
Summary: The combination of SARS-CoV-2 virus-like particle (VLP) vaccine with inactivated influenza A virus provides protection against COVID-19, influenza, and co-infection, inducing strong immune responses and neutralization activity.
Article
Engineering, Biomedical
Eunice Chee, Emily Mihalko, Kimberly Nellenbach, Jennifer Sollinger, Ke Huang, Mason Hon, Sanika Pandit, Ke Cheng, Ashley Brown
Summary: Platelet functionality relies on their conditional shape change, and thrombin-sensitive platelet-like particles (TS-PLPs) have been developed to enhance hemostasis and wound healing.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Review
Biotechnology & Applied Microbiology
Jiahui Chen, Xiaoqi Tang, Ziyu Wang, Arielle Perez, Benjamin Yao, Ke Huang, Yang Zhang, Martin W. W. King
Summary: Postsurgical adhesions are a common complication of surgical procedures and can lead to various complications. Current prevention agents are limited in their effectiveness and there is a need for further research to develop new therapies. The understanding of the cellular events associated with adhesion formation may provide insights for future prevention methods.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Materials Science, Multidisciplinary
Kristen D. Popowski, Adele Moatti, Grant Scull, Dylan Silkstone, Halle Lutz, Blanca Lopez de Juan Abad, Arianna George, Elizabeth Belcher, Dashuai Zhu, Xuan Mei, Xiao Cheng, Megan Cislo, Asma Ghodsi, Yuheng Cai, Ke Huang, Junlang Li, Ashley C. Brown, Alon Greenbaum, Phuong-Uyen C. Dinh, Ke Cheng
Summary: Respiratory diseases are a major global burden, and the development of effective therapeutics is limited. Researchers have developed a stable inhalable form of extracellular vesicles that can effectively deliver drugs to the lungs and have shown promising results in vaccine applications.
