Article
Biotechnology & Applied Microbiology
Syeed Md Iskander, Yamrot M. Amha, Phillip Wang, Qin Dong, Juhe Liu, Michael Corbett, Adam L. Smith
Summary: Co-digestion of fats, oils, and grease (FOG) with food waste (FW) can improve energy recovery in anaerobic membrane bioreactors (AnMBRs), especially by introducing more biogas production through a two-phase (TP-MP) reactor. Co-digestion of FW with FOG can also significantly increase daily biogas production and have a positive impact on microbial communities.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Food Science & Technology
Jieying Deng, Hui Xu, Xiaomin Li, Ying Wu, Baocai Xu
Summary: This study analyzed the changes in volatile flavor compounds and microbial communities in post-ripening Jinhua ham. Hexanal and nonanal were identified as the main flavor substances in Jinhua ham, and their content increased during the post-ripening stage. High-throughput sequencing revealed that Aspergillus, Saccharomyces, Staphylococcus, and Cobetia were the dominant microorganisms in post-ripening Jinhua ham. The correlation analysis showed a positive relationship between Saccharomyces, Aspergillus, Staphylococcus, and the flavor of Jinhua ham during the post-ripening stage.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Environmental Sciences
Binghan Xie, Heng Liang, Hong You, Shihai Deng, Zhongsen Yan, Xiaobin Tang
Summary: This study investigated the degradation of SMX and the response of microbial communities to different initial concentrations of SMX in MFCs. It was found that the highest SMX removal efficiency was achieved at 5 mg/L, while high concentration of SMX inhibited electron transfer. The 16S rRNA high-throughput sequencing revealed significant shifts in anode biofilm communities with different initial SMX concentrations in MFCs.
Article
Soil Science
Angela Celis de Almeida Lopes, Lucas William Mendes, Karla Annielle da Silva Bernardo Brito, Josieli Lima da Silva, Sandra Mara Barbosa Rocha, Jadson Emanuel Lopes Antunes, Louise Melo de Souza Oliveira, Vania Maria Maciel Melo, Francisca Andrea Silva Oliveira, Arthur Prudencio de Araujo Pereira, Gerson do Nascimento Costa, Veronica Brito da Silva, Regina Lucia Ferreira Gomes, Francisco de Alcantara Neto, Ademir Sergio Ferreira Araujo
Summary: This study found that the rhizospheric microbial community differs between plant species from the Phaseolus genus and these differences could be related to phylogenetic differences. The microbial richness and diversity also vary among these plant species, with P. lunatus having the most distinct rhizospheric community.
APPLIED SOIL ECOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Jingya Zhou, Shouyi Yin, Qionglin Fu, Qingqing Wang, Qing Huang, Junfeng Wang
Summary: The study found that factors such as sulphate, volatile fatty acids, and chemical oxygen demand affect the structure and distribution of microbial communities in high-salt environments, leading to concrete corrosion. The sulphur functional microorganism Desulfomicrobium and eosinophilic sulphur-oxidising bacteria play important roles in concrete corrosion, causing damage to the concrete structure through their combined actions.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2021)
Article
Ecology
Tianxiao Li, Youzhen Cai, Qinglin Ma
Summary: In this study, the microbial communities colonized on historical monuments in different microenvironments in Lingyan Temple were characterized. The results showed significant differences in microbial diversity among different historical sites. Environmental factors such as water and airborne microorganisms were found to be the main causes influencing microbial distribution.
Article
Biotechnology & Applied Microbiology
Cedric Perez, Christine Lors, Benjamin Erable
Summary: Biofilms are the predominant living presence of microorganisms on surfaces of cementitious materials, and their formation can lead to biodeterioration. Extensive research studies have been conducted to understand the basic mechanisms of biofilm formation and material deterioration, resulting in the development of analytical techniques to describe the chemical compositions, structural characteristics, and microbial diversity of these biofilms. This review article presents the latest technical developments and critically analyzes the use and limitations of these techniques, providing valuable insights for the scientific community. Additionally, new methods from other fields of microbial biofilm investigation are discussed, emphasizing the importance of physico-chemical characterization in understanding the interactions between biofilms and cementitious materials.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2022)
Article
Food Science & Technology
Xiaoyu Xu, Yuanyuan Miao, Huan Wang, Juan Du, Chenqiang Wang, Xuewei Shi, Bin Wang
Summary: This study utilized high-throughput sequencing and high-performance liquid chromatography to investigate the diversity of epiphytic microbial communities and physicochemical indicators in different wine grape varieties. The results showed that the grape variety significantly influences the structure of surface microbial communities.
Article
Biophysics
Leslie K. Daille, Javiera Aguirre, Javiera Anguita, Carlos Galarce, Luis Caro-Lara, Francisco Armijo, Ignacio T. Vargas, Gonzalo Pizarro, Magdalena Walczak, Rodrigo De la Iglesia
Summary: The composition of the passive film on alloys affects the selection of microbial biofilms, but the mechanism is not fully understood or incorporated into the analysis of corrosive biofilms. The degradation of stainless steel exposed to natural seawater was studied to investigate how the oxide layer composition influences the bacterial community. Austenitic and superferritic stainless steel grades were exposed to natural seawater, and the corrosion and biofilm formation were monitored. Bacterial communities were analyzed through massive sequencing, and the results showed that the similarity of bacterial community correlated with the variability of corrosion rate, with higher representation of certain bacterial families in stainless steels with an increased Fe/Cr ratio. Iron content in the oxide layer is found to be a key feature in selecting bacterial assemblages in marine environments.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Plant Sciences
Marc W. Schmid, Sofia J. van Moorsel, Terhi Hahl, Enrica De Luca, Gerlinde B. De Deyn, Cameron Wagg, Pascal A. Niklaus, Bernhard Schmid
Summary: The diversity and interactions between plant and soil microbial communities are complex, with plant community history and soil legacy significantly influencing soil microbial composition. Specific plant species compositions are associated with particular soil microbial taxa, impacting below-ground community composition in re-assembled plant communities.
JOURNAL OF ECOLOGY
(2021)
Article
Engineering, Environmental
Cedric Perez, Christine Lors, Pascal Floquet, Benjamin Erable
Summary: The study aims to investigate the role of biofilm in the biodeterioration of cementitious materials during anaerobic digestion. Results show the heterogeneity of microbial populations within the biofilm and their relationship to the reactive medium in anaerobic digestion.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Microbiology
Luca Marcolungo, Alessandro Passera, Simone Maestri, Elena Segala, Massimiliano Alfano, Francesca Gaffuri, Giovanni Marturano, Paola Casati, Piero Attilio Bianco, Massimo Delledonne
Summary: Rapid and sensitive diagnostic assays for plant pathogens are essential for crop disease management. This study established and evaluated a novel nanopore sequencing-based diagnostic assay, which can detect and characterize quarantine pathogens simultaneously, achieving detection and typing within 13 minutes.
Article
Microbiology
Hiroto Miura, Masayuki Takeda, Megumi Yamaguchi, Yoshihisa Ohtani, Go Endo, Yasuhisa Masuda, Kaede Ito, Yoshio Nagura, Kunihiro Iwashita, Tomohiro Mitani, Yutaka Suzuki, Yasuo Kobayashi, Satoshi Koike
Summary: This study established a high-resolution and high-throughput rumen microbial profiling approach by combining the MinION platform and buccal swab samples, which could potentially be applied in large-scale studies for rumen microbial analysis.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Environmental Sciences
Duanyi Huang, Rui Xu, Xiaoxu Sun, Yongbin Li, Enzong Xiao, Zhimin Xu, Qi Wang, Pin Gao, Zhaohui Yang, Hanzhi Lin, Weimin Sun
Summary: This study investigates the effects of Perfluorooctanoic acid (PFOA) on the microbial communities of activated sludge in wastewater treatment plants. The results show that continued exposure to PFOA leads to a decrease in microbial richness and substantial shifts in bacterial diversity and composition under both aerobic and anaerobic conditions.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Environmental Sciences
Shaoyi Jiang, Bowen Sun, Renbin Zhu, Chenshuai Che, Dawei Ma, Runfang Wang, Haitao Dai
Summary: The structure of airborne microbial communities is influenced by environmental factors, and bacteria and fungi mainly originate from soil, plants, human and animal feces. Bacterial abundance and diversity vary in different seasons and different sizes of PM.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Materials Science, Coatings & Films
A. Kityk, V. Protsenko, F. Danilov, L. Bobrova, M. Hnatko, V. Pavlik, J. Soltys, M. Labudov, M. Ruskov, D. Pangallo
Summary: This study demonstrates the possibility of designing surface properties of Ti-6Al-4V alloy through galvanostatic electrochemical treatment in Ethaline. The treatment provides controllable oxide films, improved corrosion resistance, and biologically-inspired surface topography. Additionally, the electrolyte used in this study does not cause oxidation during the electrochemical processing, and the treated samples exhibit good cell adhesion and viability.
SURFACE & COATINGS TECHNOLOGY
(2022)
Article
Parasitology
Alzbeta Radacovska, Eva Cisovska Bazsalovicsova, Katarina Soltys, Jan Stefka, Gabriel Minarik, Andrea Gustinelli, Julia K. Chugunova, Ivica Kralova-Hromadova
Summary: This study analyzed genes and microsatellite loci to explore how the recently detected triploidy/parthenogenesis in tapeworms from the Alpine lakes region (ALR) is displayed at the DNA level. The results showed that the tapeworms from ALR had higher mitochondrial haplotype diversity compared to a geographically distant population from Russia. This suggests that the diploid/sexually reproducing population in Russia is the ancestral population, while the triploid/parthenogenetically reproducing population in ALR is at the margin of the distribution.
Article
Biology
Simona Ugrayova, Peter Svec, Ivan Hric, Sara Sardzikova, Libusa Kubanova, Adela Penesova, Jaroslava Adamcakova, Petra Pacesova, Julia Horakova, Alexandra Kolenova, Katarina Soltys, Martin Kolisek, Viktor Bielik
Summary: This study investigates the effects of hematopoietic stem cell transplantation (HSCT) treatment on the gut microbiota composition in children with acute lymphoblastic leukemia (ALL). The results show that HSCT treatment negatively affects the bacterial diversity and richness of the gut microbiota, leading to the dominance of pathogenic bacteria. However, it is promising to note that there is an association between bacterial diversity and physical exercise during HSCT treatment. These findings provide additional support for the importance of studying the gut microbiome in children with cancer.
Article
Plant Sciences
Michaela Mrkvova, Richard Hancinsky, Lukas Predajna, Peter Alaxin, Adam Achs, Jana Tomasechova, Katarina Soltys, Daniel Mihalik, Antonio Olmos, Ana Belen Ruiz-Garcia, Miroslav Glasa
Summary: Cucumber mosaic virus (CMV) is a widely distributed and genetically variable virus. High-throughput sequencing was used to analyze the genomes of 14 Slovak CMV variants from different plant hosts. A total of three CMV variants were found in Papaveraceae species, which were previously not well-described as CMV natural hosts. Phylogenetic analysis divided the Slovak CMV isolates into two genetically distinct groups, Ia and II. The SL50V variant with a divergent RNA2 sequence may be a reassortant variant. Satellite CMV RNA was also identified in four samples, but its role in observed symptoms could not be determined due to complex infections with different viruses in the original hosts.
Article
Biochemistry & Molecular Biology
Monika Hofbauerova, Magdalena Ruskova, Andrea Puskarova, Maria Buckova, Adriana Annusova, Eva Majkova, Peter Siffalovic, Giuseppe Granata, Edoardo Napoli, Corrada Geraci, Domenico Pangallo
Summary: The colonization of building materials by microorganisms is a major cause of degradation, posing health risks from fungal and bacterial products. The study evaluated the efficacy of oregano and thyme essential oils encapsulated in nanocapsules for protection and disinfection against various microorganisms. The results showed that these nanocapsules effectively disinfect and inhibit biofilm formation without causing visible color changes in the materials.
Article
Virology
Lucie Slavikova, Emad Ibrahim, Glenda Alquicer, Jana Tomasechova, Katarina Soltys, Miroslav Glasa, Jiban Kumar Kundu
Summary: Turnip yellows virus is an important pathogen of oilseed rape worldwide, infecting various crop species and weeds. We detected the virus in many commonly grown weed species in Czech and Slovak Republics. We discovered four new weed species as hosts of the virus. Genetic analysis showed similarity among different isolates and no geographical or host-based clustering.
Article
Food Science & Technology
Matej Plany, Jozef Sitarcik, Jelena Pavlovic, Jaroslav Budis, Janka Korenova, Tomas Kuchta, Domenico Pangallo
Summary: The use of third generation sequencing technologies allows for longer sequence reads, making it possible to use the longest amplicons as specific bacterial markers for metabarcoding studies. Nanopore sequencing technologies are increasingly used worldwide to analyze microbiomes in environmental and food samples. This study optimized a PCR-based method for sequencing almost the entire ribosomal operon sequences and applied it to the analysis of bacterial consortia in dairy fermented products.
Article
Biochemistry & Molecular Biology
Silvia Toporcerova, Ivana Spakova, Katarina Soltys, Zuzana Klepcova, Marek Kl'oc, Julia Bohosova, Karolina Trachtova, Lucia Peterova, Helena Mickova, Peter Urdzik, Maria Marekova, Ondrej Slaby, Miroslava Rabajdova
Summary: The increased interest in assisted reproduction through in vitro fertilization (IVF) has created a need for biomarkers that can highly predict the success of pregnancy. Researchers have found that analyzing short non-coding RNA (sncRNA) molecules secreted during the IVF process using artificial intelligence can enhance our understanding of communication between embryonic cells and the endometrium, leading to increased success rates.
Review
Biotechnology & Applied Microbiology
Patricia Sanmartin, Pilar Bosch-Roig, Domenico Pangallo, Lucia Krakova, Miguel Serrano
Summary: The organisms inhabiting built cultural heritage can passively or actively participate in conservation processes. Active participants are focused on for their positive roles in various processes, as well as providing ecosystem services. These organisms can also evolve or mutate in response to changes and acquire new capacities. Recent studies have opened up new avenues of research involving a wide variety of organisms and viruses, which are expected to gain more attention in the future.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Bence Farkas, Hana Vojtkova, Zuzana Farkas, Domenico Pangallo, Peter Kasak, Antonio Lupini, Hyunjung Kim, Martin Urik, Peter Matus
Summary: Manganese oxides are crucial in natural geochemical barriers, as they have reactive properties towards essential and potentially toxic trace elements. Microorganisms can actively alter conditions in their microenvironment and dissolve minerals, as well as precipitate bioavailable manganese ions into biogenic minerals. These microbial transformations significantly impact the geochemistry of manganese and elements associated with its oxides, potentially leading to environmental consequences.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Magdalena Ruskova, Alena Opalkova Siskova, Katarina Mosnackova, Custodia Gago, Adriana Guerreiro, Maria Buckova, Andrea Puskarova, Domenico Pangallo, Maria Dulce Antunes
Summary: This study focuses on encapsulating essential oils (lemongrass and oregano) into PLA and PHB packaging films and evaluating their antimicrobial and antioxidant properties for strawberry preservation and shelf-life extension. The results show that the tested active packaging film with essential oils can effectively maintain the quality and extend the shelf life of strawberries, with PLA/PHB/ATBC + 5% lemongrass essential oil performing slightly better than PLA/PHB/ATBC + 5% oregano essential oil.
Article
Environmental Sciences
Magdalena Ruskova, Maria Buckova, Andrea Puskarova, Marianna Cichova, Veronika Janska, Adam Achs, Zdeno Subr, Tomas Kuchta, Domenico Pangallo
Summary: Wastewater monitoring is an important approach for detecting and controlling the SARS-CoV-2 pandemic. In this study, a one-step single-tube nested qRT-PCR assay was developed to increase the sensitivity of qRT-PCR. The assay demonstrated higher detection rates and robustness in samples with low viral loads.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Chemistry, Multidisciplinary
Jelena Pavlovic, Zuzana Farkas, Lucia Krakova, Domenico Pangallo
Summary: Fungi are the main contaminants of books and archival documents, and they can degrade paper and produce pigments. Synthetic colors on paper can also be degraded by enzymes. This review explores the potential of enzymes in removing fungal and synthetic pigments, and discusses their potential applications.
APPLIED SCIENCES-BASEL
(2022)