Review
Chemistry, Multidisciplinary
Hossein Rahimi, Marziyeh Salehiabar, Murat Barsbay, Mohammadreza Ghaffarlou, Taras Kavetskyy, Ali Sharafi, Soodabeh Davaran, Subhash C. Chauhan, Hossein Danafar, Saeed Kaboli, Hamed Nosrati, Murali M. Yallapu, Joao Conde
Summary: The emergence of COVID-19 in 2019 has become a global pandemic, leading to serious concerns worldwide. The development of CRISPR-based diagnostic systems has reshaped molecular diagnosis and inspired researchers to develop more efficient methods for COVID-19 detection and treatment. The advantages of CRISPR-based COVID-19 diagnostic systems include high detection speed, sensitivity, precision, portability, and the elimination of the need for specialized laboratory equipment.
Review
Virology
Xiaojian Cui, Zhihu Zhao, Tongqiang Zhang, Wei Guo, Wenwei Guo, Jiafeng Zheng, Jiayi Zhang, Cuicui Dong, Ren Na, Lisheng Zheng, Wenliang Li, Zihui Liu, Jia Ma, Jinhu Wang, Sijia He, Yongsheng Xu, Ping Si, Yongming Shen, Chunquan Cai
Summary: This study provides a comprehensive analysis of demographic characteristics, clinical symptoms, laboratory findings, and imaging features of COVID-19 in pediatric patients. It revealed that children with COVID-19 tend to have mild illness with typical manifestations of fever and cough, normal white blood cell counts, and common imaging features of normal or ground-glass opacity. However, the high incidence of critical cases and vomiting symptoms in children under 1 year old should be given attention.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Immunology
Grace Chung-Yan Lui, Terry Cheuk-Fung Yip, Vincent Wai-Sun Wong, Viola Chi-Ying Chow, Tracy Hang-Yee Ho, Timothy Chun-Man Li, Yee-Kit Tse, Henry Lik-Yuen Chan, David Shu-Cheong Hui, Grace Lai-Hung Wong
Summary: A territory-wide cohort study in Hong Kong compared the case-fatality ratios of COVID-19 and SARS, finding a much lower CFR for COVID-19 at 0.4% compared to 17.1% for SARS. Age and diabetes were associated with worse outcomes in COVID-19 patients, while antiviral treatments showed no significant association with the primary endpoint.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Immunology
Adarsh Bhimraj, Rebecca L. Morgan, Amy Hirsch Shumaker, Lindsey Baden, Vincent Chi Chung Cheng, Kathryn M. Edwards, Jason C. Gallagher, Rajesh T. Gandhi, William J. Muller, Mari M. Nakamura, John C. O'Horo, Robert W. Shafer, Shmuel Shoham, M. Hassan Murad, Reem A. Mustafa, Shahnaz Sultan, Yngve Falck-Ytter
Summary: This article introduces the guidelines developed by the Infectious Diseases Society of America for the treatment and management of COVID-19. The guideline panel regularly reviews the evidence and uses the GRADE approach to assess the certainty of evidence and make recommendations. The panel has made 32 recommendations for different groups/populations, including prophylaxis, ambulatory patients with mild-to-moderate disease, and hospitalized patients. These are living guidelines and the most recent recommendations can be found on the specified website.
CLINICAL INFECTIOUS DISEASES
(2022)
Review
Chemistry, Analytical
Sulaiman Alsalameh, Khalid Alnajjar, Tariq Makhzoum, Noor Al Eman, Ismail Shakir, Tanveer Ahmad Mir, Khaled Alkattan, Raja Chinnappan, Ahmed Yaqinuddin
Summary: This article reviews the recent advances and advantages of using biosensors for COVID-19 diagnosis. Traditional methods have limitations, while biosensors are sensitive, specific, rapid, and user-friendly, with the potential to go beyond traditional standards in diagnostics.
Review
Biotechnology & Applied Microbiology
Praveen Rai, Ballamoole Krishna Kumar, Vijaya Kumar Deekshit, Indrani Karunasagar, Iddya Karunasagar
Summary: COVID-19 is a disease caused by SARS-CoV-2 that has spread globally, requiring collaborative efforts for accurate diagnosis and prevention. While RT-qPCR detection is considered the gold standard for early virus detection, challenges such as technical complexity and limited applicability have led to the development of portable POC tests.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Virology
Reza Soleimani, Corentin Deckers, Te-Din Huang, Pierre Bogaerts, Stephanie Evrard, Isaline Wallemme, Boutaina Habib, Pauline Rouze, Olivier Denis
Summary: This study evaluated the performances of two rapid antigen testing devices in detecting SARS-CoV-2 in nasopharyngeal samples, showing relatively high sensitivity, especially with lower cycle threshold values, and 100% specificity for both devices.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Roghayeh Sheervalilou, Milad Shirvaliloo, Saman Sargazi, Sakine Shirvalilou, Omolbanin Shahraki, Younes Pilehvar-Soltanahmadi, Alireza Sarhadi, Ziba Nazarlou, Habib Ghaznavi, Samideh Khoei
Summary: COVID-19, caused by SARS-CoV-2, has become a major topic of discussion in the new decade due to its high transmission rate. With no vaccine or specific treatment available, the focus has shifted to traditional methods like quarantine, combined with early diagnosis using biosensors. Nanobiotechnology has introduced biosensors as a versatile alternative to traditional diagnostics, leading to accurate identification of analytes and the advancement of portable biosensors for diagnostic use in healthcare.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Zulkar Nain, Humayan Kabir Rana, Pietro Lio, Sheikh Mohammed Shariful Islam, Matthew A. Summers, Mohammad Ali Moni
Summary: This study revealed genetic linkage between 2019-nCoV and SARS-CoV through whole-genome alignment and gene expression analysis, identified shared genes related to COVID-19 complications, and suggested potential drug candidates for combating COVID-19 based on protein-chemical interactions.
BRIEFINGS IN BIOINFORMATICS
(2021)
Review
Green & Sustainable Science & Technology
Hafiza Salaha Mahrosh, Ghulam Mustafa
Summary: This study aims to comprehensively discuss various aspects related to the novel coronavirus, including genomic, epidemiological, social, clinical, and environmental aspects, as well as potential vaccine candidates against SARS-CoV-2. Despite multiple vaccine candidates in evaluation, none has been validated with significant efficacy against the disease, highlighting the urgent need for the development of an effective vaccine.
ENVIRONMENT DEVELOPMENT AND SUSTAINABILITY
(2021)
Article
Immunology
Rebecca N. Kumar, En-Ling Wu, Valentina Stosor, William J. Moore, Chad Achenbach, Michael G. Ison, Michael P. Angarone
Summary: A retrospective case-control study found that treatment with bamlanivimab was associated with a lower 30-day hospitalization rate in high-risk ambulatory patients with COVID-19.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Marc-Antoine de la Vega, Ara Ara Xiii, Marc F. Lee, Gary P. Kobinger
Summary: A case study from Canada demonstrates the effectiveness of conducting real-time PCR-based detection of SARS-CoV-2 in a diagnostic laboratory at the point of travel, which helps identify infected individuals and protect at-risk communities.
SCIENTIFIC REPORTS
(2022)
Editorial Material
Biochemistry & Molecular Biology
Stephen Bustin, Reinhold Mueller, Gregory Shipley, Tania Nolan
Summary: This commentary aims to clarify the true strengths and limitations of molecular testing technology, especially RT-qPCR, and to address and help stop the unfounded speculation surrounding its use.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Yun Lin, Bingyi Yang, Sarah Cobey, Eric H. Y. Lau, Dillon C. Adam, Jessica Y. Wong, Helen S. Bond, Justin K. Cheung, Faith Ho, Huizhi Gao, Sheikh Taslim Ali, Nancy H. L. Leung, Tim K. Tsang, Peng Wu, Gabriel M. Leung, Benjamin J. Cowling
Summary: Analysis of viral load data on confirmed cases can improve real-time estimation of the reproductive number, enabling more timely tracking of epidemic dynamics.
NATURE COMMUNICATIONS
(2022)
Review
Medicine, General & Internal
Debashis Dutta, Sarah Naiyer, Sabanaz Mansuri, Neeraj Soni, Vandana Singh, Khalid Hussain Bhat, Nishant Singh, Gunjan Arora, M. Shahid Mansuri
Summary: This article reviews the natural history, pathology, molecular biology, and efficient diagnostic methods of COVID-19, with a focus on the gold standard RT-qPCR method for COVID-19 diagnosis. It emphasizes the importance of the COVID-19 pandemic and aims to raise awareness about the disease and its management.
Article
Virology
Jong-Hwan Lee, Yujin Jung, Sung-Kyun Lee, Jung Kim, Chang-Seop Lee, Soohyun Kim, Ji-Seon Lee, Nam-Hoon Kim, Hong-Gi Kim
Summary: This study developed specific monoclonal antibodies for the rapid detection of SARS-CoV-2 NP. The rapid biosensor was able to detect cultured virus samples and recombinant NP of SARS-CoV-2, and was validated with clinical specimens. This rapid biosensor could be important for detecting SARS-CoV-2 NP in the presence of spreading variants.
Article
Chemistry, Multidisciplinary
Jung Kim, Sung Kyun Lee, Jong-Hwan Lee, Hye-Yeon Kim, Nam Hoon Kim, Chang Hoon Lee, Chang-Seop Lee, Hong Gi Kim
Summary: A novel serological assay based on ZnO-NW MP has been developed to detect early antibody responses to SARS-CoV-2 in asymptomatic patients with high sensitivity, enabling accurate diagnosis and estimation of the epidemiology of COVID-19.
ADVANCED MATERIALS INTERFACES
(2022)
Article
Engineering, Chemical
Geum-Seok Jeong, Eun-Young Lee, Myung-Gyun Kang, Sang-Jip Nam, Daeui Park, Hoon Kim
Summary: This study evaluated the inhibitory activities of lichen fungi against human monoamine oxidases (hMAOs). A compound called (S)-5-methylmellein (5MM) was isolated from an extract and exhibited strong inhibitory activity against hMAO-A. It also showed moderate inhibitory activity against acetylcholinesterase. Molecular docking analysis indicated that 5MM had higher binding affinity for hMAO-A compared to hMAO-B. In silico pharmacokinetic analysis suggests that 5MM has good absorption and permeability properties. These findings suggest that 5MM may be a potential candidate for the treatment of neuropsychiatric disorders.
Article
Biophysics
Jong-Hwan Lee, Yungmin Lee, Sung Kyun Lee, Jung Kim, Chang-Seop Lee, Nam Hoon Kim, Hong Gi Kim
Summary: The study developed an ACE2-based biosensor that can detect S1 mutations and neutralizing antibodies of SARS-CoV-2. The biosensor can detect various variants and the wild-type SARS-CoV-2, distinguishing them based on color differences. This approach will facilitate the development of various biosensors against SARS-CoV-2.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Medicinal
Sungbo Hwang, Seung-Hwa Baek, Daeui Park
Summary: This study proposes a method that combines statistical analysis and molecular quantum mechanics simulations to efficiently predict the binding affinity between SARS-CoV-2 variants and hACE2 or monoclonal antibodies. The method accurately predicts the trend of binding affinity and interaction energy changes, demonstrating its effectiveness in predicting the effects of mutations.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)
Article
Developmental Biology
Jinsoo Lee, Ji-Seong Jeong, Sang Yun Kim, Woojin Kim, Sun-Young Lee, Jeong-Dong Park, Jung-Hwa Oh, Daeui Park, Seung-Jin Lee, Sang-Ki Baek, Yixian Quah, Sang-Yoon Nam, Wook-Joon Yu
Summary: This study investigated and characterized an animal model for bis-diamine-induced congenital heart defects. The results demonstrate the potential clinical value of this animal model and its usefulness in further studies on cardiovascular defects and related mechanisms.
BIRTH DEFECTS RESEARCH
(2022)
Article
Biochemical Research Methods
Hyun Kil Shin, Hang-Suk Chun, Sangwoo Lee, Se-Myo Park, Daeui Park, Myung-Gyun Kang, Sungbo Hwang, Jung-Hwa Oh, Hyoung-Yun Han, Woo-Keun Kim, Seokjoo Yoon
Summary: Drug-induced liver injury poses challenges in predictive toxicology due to complex metabolites and various mechanisms involved. ToxSTAR offers structural similarity-based analysis and prediction models for different DILI subtypes.
Article
Biochemistry & Molecular Biology
Dong-Eun Kim, Young Hyun Shin, Jung-Eun Cho, Subeen Myung, Hong Gi Kim, Kyung-Chang Kim, Chul Min Park, Cheol-Hee Yoon
Summary: This study synthesized a series of novel 3-oxindole-2-carboxylates and evaluated their antiviral activity against HIV-1 infection. One compound showed the most potent inhibitory effect without severe cytotoxicity. Mode-of-action studies indicated that these compounds specifically inhibited Tat-mediated viral transcription.
Article
Toxicology
Heeyoung Yang, Tamina Park, Daeui Park, Myung-Gyun Kang
Summary: Drug-induced liver injury (DILI) is a serious concern for public healthcare systems and pharmaceutical companies. This study investigates the mechanism by which DILI drugs alter the activation and phenotype of hepatic macrophages. The findings suggest that proinflammatory M1-like macrophages contribute to hepatotoxicity mediated by DILI drugs, and the interferon type I pathway, cytokines, and apoptosis pathway are involved in the initiation of liver injury. Transcriptional changes in genes related to macrophage polarization could be used as indicators to reflect the severity of DILI during drug development.
TOXICOLOGY IN VITRO
(2022)
Article
Gastroenterology & Hepatology
Ji-Yoon Noh, In Pyo Lee, Na Rae Han, Miok Kim, Yong Ki Min, Sang-Yeop Lee, Sung Ho Yun, Seung Il Kim, Tamina Park, Hyunmin Chung, Daeui Park, Chang Hoon Lee
Summary: In this study, a novel combination therapy of AB680 and palbociclib was demonstrated to be advantageous for the treatment of CRC.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sang Gyun Noh, Hee Jin Jung, Seungwoo Kim, Radha Arulkumar, Dae Hyun Kim, Daeui Park, Hae Young Chung
Summary: This study aimed to evaluate the effects of age and sex on gene expression and identify sex-specific target molecules in the regulation of aging. The results showed that the expression of genes related to circadian rhythms changed more in males than in females during liver aging, and Nr1d1 and Nr1d2 were upregulated in males. These findings suggest that age-related upregulation of Nr1d1/2 may play a more crucial role in the change in circadian rhythms in males during liver aging.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jong Min Oh, Hyun-Jae Jang, Myung-Gyun Kang, Seul-Ki Mun, Daeui Park, Su-Jin Hong, Min Ha Kim, Soo-Young Kim, Sung-Tae Yee, Hoon Kim
Summary: Thirteen compounds were isolated from Canavalia lineata pods and their inhibitory activities against hMAO-A and -B were evaluated. Compounds 8 and 13 showed potent inhibitory activity against hMAO-B, while most compounds weakly inhibited MAO-A. Compounds 8 and 13 were reversible competitive inhibitors against hMAO-B.
Article
Nanoscience & Nanotechnology
Yujin Jung, Jung Kim, Nam Hoon Kim, Hong Gi Kim
Summary: Fluorescence enhancement, including metal-enhanced fluorescence (MEF), has been widely explored in biosensor technology. In this study, a novel 3D MEF substrate using Ag-ZnO nanocomplex was developed, showing significant fluorescence enhancement compared to the glass substrate. The Ag-ZnO nanocomplex exhibited a 50-fold higher sensitivity for the detection of double-stranded DNA compared to a 2D MEF substrate. This research provides a potential platform for the development of sensitive MEF-based biosensors for DNA detection.
ACS APPLIED NANO MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Young Hyun Shin, Dong-Eun Kim, Kyung Lee Yu, Chul Min Park, Hong Gi Kim, Kyung-Chang Kim, Songmee Bae, Cheol-Hee Yoon
Summary: Designed a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) assay using europium cryptate as a fluorescence donor to investigate the interaction between Tat and TAR RNA for HIV-1 replication. The optimized assay showed specificity and successfully identified two small molecules (460-G06 and 463-H08) capable of inhibiting Tat activity and HIV-1 infection. This assay is suitable for high-throughput screening and the identified compounds may serve as potential molecular scaffolds for new HIV-1 drug development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Soohyun Kim, Sung Kyun Lee, Ahryeong Son, Jong-Hwan Lee, Hong Gi Kim
Summary: Chronic obstructive pulmonary disease (COPD) is a deadly lung disease with limited drug discovery due to lack of appropriate preclinical models. Researchers have developed a comparative inflammation-on-a-chip (IoC) model that mimics the key feature of COPD, neutrophil transendothelial migration (NTEM). Using this model, the effects of CXCR2 inhibitors on neutrophil migration in the presence of COPD patient samples were evaluated. This IoC model provides a valuable tool for studying COPD severity and advancing research on NTEM.
ADVANCED HEALTHCARE MATERIALS
(2023)
