Article
Biochemistry & Molecular Biology
Aojie Cai, Nana Liu, Zehong Lin, Xiao Li, Jingmin Wang, Ye Wu, Kai Gao, Yuwu Jiang
Summary: This study investigated the role of Acitretin in neuronal differentiation using the SH-SY5Y neuroblastoma cell line. The results showed that Acitretin effectively promoted the differentiation of SH-SY5Y cells into neuronal cells and upregulated the expression of neuronal markers. RNA sequencing analysis identified significant changes in genes associated with neuron development. Whole-cell patch clamping and microelectrode array assays demonstrated that Acitretin-treated neurons exhibited electrical activity similar to mature neurons. This study provides evidence for a reliable neuron-like cell model and validates the potential of Acitretin in neuronal differentiation and the treatment of related diseases.
NEUROCHEMICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Bahaa Daou, Alessandro Silvestri, Haizpea Lasa, Donato Mancino, Maurizio Prato, Nuria Alegret
Summary: Carbon nanotubes (CNT) have been found to be effective substrates for neuronal cultures, enhancing synaptic functionality. In this study, multiwalled CNT (f-CNT) functionalized with different groups are used as substrates for neuroblastoma cell incubation. Cell viability assays show increased proliferation on various f-CNT substrates. The type of functionalization also affects cell differentiation and morphology.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Biochemical Research Methods
Samiksha Sardana, Anneroos E. Nederstigt, Marc P. Baggelaar
Summary: S-palmitoylation is a lipid modification that plays a role in neuronal development and neurodegenerative diseases. In this study, two methods were used to identify S-palmitoylated proteins and sites during neuronal differentiation. A subset of high-confidence S-palmitoylated proteins were identified, suggesting an important role for S-palmitoylation in neuronal differentiation.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
D. Merryweather, S. R. Moxon, A. J. Capel, N. M. Hooper, M. P. Lewis, P. Roach
Summary: Cellular metabolism and behaviour are closely linked to cytoskeletal tension and scaffold mechanics. In this study, a 3D culture system using diluted type I collagen hydrogel to produce variation in gel stiffness was explored. The results showed that hydrogel scaffold remodelling was linked to gel collagen concentration and different concentrations of gel led to significant changes in programmed cell behaviour and cell morphology.
Article
Nanoscience & Nanotechnology
Shintaro Yamaguchi, Ryo Isaka, Yuji Sakahashi, Hirofumi Tsujino, Yuya Haga, Kazuma Higashisaka, Yasuo Tsutsumi
Summary: Research has found that silver nanoparticles with a diameter of 10 nm can inhibit retinoic acid-induced neuronal differentiation. This inhibitory effect may be achieved through the oxidative stress pathway, induction of mitochondrial ROS production, and decreased expression of mitochondrial fusion-related genes.
ACS APPLIED NANO MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Sneha Singh, Rishi K. Somvanshi, Ujendra Kumar
Summary: This study compared the expression of key markers in differentiated and non-differentiated SH-SY5Y cells, and explored the role of somatostatin (SST) in regulating neurotransmitter distribution and expression. The results suggest the potential therapeutic implication of SST in neurodegeneration.
Article
Toxicology
Jungeun Lee, Beate Escher, Stefan Scholz, Rita Schlichting
Summary: Early life exposure to environmental chemicals can lead to developmental neurotoxicity. Neurite outgrowth inhibition can be used as an endpoint to screen for neurotoxic effects. Hydrophilic chemicals are more likely to cause cytotoxicity and neuronal-specific toxicity, while hydrophobic chemicals tend to act through baseline toxicity.
ARCHIVES OF TOXICOLOGY
(2022)
Article
Neurosciences
Zhuo-Min Ling, Qian Wang, Yu Ma, Peng Xue, Yun Gu, Mao-Hong Cao, Zhong-Ya Wei
Summary: Reactive astrocytes play a crucial role in the inflammatory response of brain injury, including Parkinson's disease. Pannexin 1 has been identified as a membrane channel involved in substance release in neurodegenerative diseases, and in this study, upregulation of Panx 1 was associated with inflammatory cytokine expression. Moreover, inhibiting Panx 1 could suppress pro-inflammatory cytokine expression induced by lipopolysaccharide, suggesting its potential as a key regulator in reactive astrocytes.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Food Science & Technology
Akira Sato, Nozomi Tagai, Yoko Ogino, Haruka Uozumi, Shinpei Kawakami, Takayuki Yamamoto, Sei-ichi Tanuma, Hiroko Maruki-Uchida, Sadao Mori, Minoru Morita
Summary: This study found that piceatannol-rich passion fruit seed extract can suppress neuronal cell death in an Alzheimer's disease cell model by protecting against neurite fragmentation. Passion fruit seed extract and piceatannol have the potential to be neuroprotective functional foods for the prevention and treatment of Alzheimer's disease.
FOOD SCIENCE & NUTRITION
(2022)
Article
Cell Biology
Anja Pislar, Janko Kos
Summary: Neurotrophins like gamma-enolase activate various signaling pathways through specific receptors, promoting neurotrophic processes in neuronal cells. This study found that gamma-enolase associates with the intracellular domain of Trk receptors at the plasma membrane, and its neurotrophic activity is dependent on this association. Additionally, a peptide mimicking gamma-enolase activity promoted Trk receptor internalization and endosomal trafficking, demonstrating the role of Trk kinase activity in mediating neurotrophic signaling.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Nutrition & Dietetics
Chia-Ling Ho, Ning-Jo Kao, Ching- Lin, Tzu-Wen L. Cross, Shyh-Hsiang Lin
Summary: Alzheimer's disease is a common neurodegenerative disorder, with oxidative stress and mitochondrial biogenesis being implicated in its progression. Quercetin has been found to increase mitochondrial biogenesis and reduce oxidative stress-induced damage, showing promise in protecting neuronal cells.
Article
Biochemistry & Molecular Biology
Taewoo Kim, Haejun Oh, Yoonsu Jeon, Hyeyoung Shin, Juhee Lim, Changjin Lim, Jakyung Yoo, Young-Ger Suh, Seok-Ho Kim, Hyo In Kim, Hyun Su Kim, Woo Sung Son, Hyun Jin Choi
Summary: The discovery and development of small molecules that regulate specific neuronal phenotypes is crucial for treating neurological diseases. In this study, new ERRγ agonists were synthesized and their ability to enhance dopaminergic neuronal phenotypes was validated through experiments.
BIOORGANIC CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Zahra Madadi, Shiva Akbari-Birgani, Saeed Mohammadi, Mitra Khademy, Seyed Asadollah Mousavi
Summary: Neuroblastoma is a common malignant tumor in infants and young children, with differentiation-inducing potential. The activation of caspase-9 plays a crucial role in the differentiation process of human neuroblastoma cells, promoting a change in cell morphology and protein expression.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Pooja Gangras, Valentina Gelfanova, Graham D. Williams, Samuel K. Handelman, Ryan M. Smith, Marjoke F. Debets
Summary: This study identifies high-confidence surface proteins expressed on the brain and DRG neurons using a bioinformatic workflow and demonstrates significant overlap between the SH-SY5Y cell surfaceome and the surface proteome of human brain and DRG neurons. It also finds that alternative splicing of specific isoforms in 32% of shared surface proteins allows them to maintain their protein-coding ability and localize to the cell surface. Validation of one of these isoforms confirms its presence in neuroblastomas, making it a receptor of interest for neuroblastoma-specific therapeutics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Behavioral Sciences
Ping Wang, Peipei Wang, Hengfei Luan, Yong Wu, Ying Chen
Summary: The study suggests that Midazolam has potential therapeutic effects in Alzheimer's disease (AD) by reducing oligomerized amyloid beta (A beta)-associated neuronal senescence and restoring telomerase activity and gene expressions associated with cellular senescence.
BRAIN AND BEHAVIOR
(2023)