期刊
FEBS OPEN BIO
卷 10, 期 9, 页码 1737-1747出版社
WILEY
DOI: 10.1002/2211-5463.12932
关键词
cell surface markers; chemically defined medium; hepatocellular carcinoma; in vitroculture model; liver cancer stem cells
资金
- National Key Research and Development Program of China [2017YFA0103100, 2017YFA0103103, 2017YFA0103104]
- Natural Science Foundation of China [81773137]
Liver cancer stem cells (L-CSCs) are considered to be an important therapeutic target for hepatocellular carcinoma (HCC). This study provides a newin vitrolong-term culture model for a specific subpopulation of L-CSCs enriched by cell surface markers. We combined CD13, CD133 and EpCAM to selectively enrich L-CSCs, which we then cultured in modified chemically defined medium. The enriched L-CSCs exhibited enhanced proliferation, self-renewal and long-term clonal maintenance ability as compared with non-CSCs. Compared with wild-type hepatocellular carcinoma, the expression of stemness surface markers, oncogenes, drug resistance and tumorigenicity in enriched L-CSCs was significantly increased. In summary, the subpopulation of L-CSCs still maintains cancer stem cell-related phenotypes after 14 days of culture.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据