Article
Environmental Sciences
Jenet George, Devi Rajendran, P. Senthil Kumar, Srinidhi Sonai Anand, Vaidyanathan Vinoth Kumar, Gayathri Rangasamy
Summary: In this study, porous-cross-linked enzyme aggregates (CLEAs) of Pleurotus ostreatus laccase were used for the decolorization and detoxification of triarylmethane and azo dyes. The CLEAs laccase showed high efficiency in decolorizing malachite green (MG) and reactive blue 2 (RB) without a mediator, with decolorization rates of 58.33-98.12% after 120 minutes. The immobilized laccase was able to effectively remove dyes from aqueous solutions and demonstrated significant detoxification in experimental plants and plant growth-promoting rhizobacteria. This suggests that porous CLEAs laccase could be a potential tool for the bioremediation of dyeing wastewater.
Article
Chemistry, Physical
Weichuan Qiao, Ziyan Zhang, Yi Qian, Lijie Xu, He Guo
Summary: In the process of enzyme immobilization using the cross-linking method, a magnetic dialdehyde cellulose (MDAC) was developed without the need for a traditional cross-linker, showing improved enzyme loading capacity and stability. The MDAC-laccase complex exhibited enhanced properties such as a wide pH range, temperature stability, reusability, and tolerance to organic solvents, inhibitors, and metal ions. Additionally, the MDAC-L demonstrated efficient magnetic separation, good decolorization performance, and maintained high enzyme activity after multiple cycles of reuse.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Environmental Sciences
Emre Birhanli, Samir Abbas Ali Noma, Filiz Boran, Ahmet Ulu, Ozfer Yesilada, Burhan Ates
Summary: This study aims to present a simple and effective carrier matrix for immobilizing laccase and remove textile dyes. Two different metal-organic frameworks (MOFs) based on Cobalt (Co) and Copper (Cu) were prepared and laccase was successfully immobilized via encapsulation. The immobilized laccases showed good reusability, resistance to temperature denaturation, high storage stability, and improved thermostability. The MOFs also exhibited good decolorization activities for textile dyes.
Article
Biotechnology & Applied Microbiology
Nikolina Popovic, Marija Stanisic, Karla Ilic Durdic, Olivera Prodanovic, Natalija Polovic, Radivoje Prodanovic
Summary: In this study, a modified pectin was synthesized and used to produce dopamine-pectin microbeads with immobilized laccase through enzymatic crosslinking. The immobilized laccase showed improved thermal and pH stability, effectively decolorizing various dyes. The microbead immobilized laccase maintained high decolorization efficiency even after ten cycles of repeated use.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Engineering, Environmental
Yan Zhang, Thea Jess Plesner, Yi Ouyang, Yu-Cong Zheng, Etienne Bouhier, Emilie Ingemann Berentzen, Mingliang Zhang, Pengfei Zhou, Wolfgang Zimmermann, Gregers Rom Andersen, Bekir Engin Eser, Zheng Guo
Summary: A novel thermophilic laccase, LfLAC3, was identified from PE-degrading Lysinibaccillus fusiformis through computer-aided and activity-based screening. LfLAC3 showed high robustness and catalytic promiscuity. It was able to degrade all tested dyes and low-density polyethylene films without the use of a mediator, indicating its potential for dye decolorization and plastic degradation.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Murat Uygun, Victor de la Asuncion-Nadal, Sinem Evli, Deniz Akta Uygun, Beatriz Jurado-Sanchez, Alberto Escarpa
Summary: Laccase-based micromotors were synthesized and used for decolorization of azo dyes in textile wastewater, showing improved dye removal capacity compared to the free enzyme approach. The stability of the immobilized enzyme was enhanced, resulting in higher decolorization efficiencies of 76-94% compared to 34-55% using free enzyme.
APPLIED MATERIALS TODAY
(2021)
Review
Biotechnology & Applied Microbiology
Younes Adamian, Linson Lonappan, Komla Alokpa, Spiros N. Agathos, Hubert Cabana
Summary: The immobilization of laccase, an enzyme with potential environmental applications, is crucial for enhancing its industrial potential, due to issues of stability and reusability. Carbon-based materials are explored as ideal support candidates for laccase immobilization, providing high porosity, surface area, functional groups, and aromatic structure. This study reviews recent trends in laccase production and immobilization techniques, highlighting the significant benefits of carbon-based materials in enzyme immobilization.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Hongrao Wang, Shuran Han, Jiayi Wang, Shuyu Yu, Xiaoyan Li, Lei Lu
Summary: The immobilized laccase as magnetic cross-linked enzyme aggregates (M-CLEAs) showed promising activity recovery and stability for dye decolorization, especially under alkaline conditions. Additionally, M-CLEAs exhibited enhanced tolerance towards NaCl and were able to rapidly decolorize various dyes.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Engineering, Environmental
Jian Peng, Enhui Wu, Xiaoxuan Lou, Qian Deng, Xiaohong Hou, Chongning Lv, Qi Hu
Summary: In this study, a MOF/PVA/Lac composite with excellent enzymatic properties for degrading AQs and removing alizarin green efficiently was synthesized. The degradation product p-toluidine was identified using molecular docking, mass spectrometry, and 2DNMR, confirming the reaction mechanism C-alpha oxidation.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Engineering, Environmental
Rabab Salih, Katarina Banjanac, Ana Vukoicic, Jelena Grzetic, Ana Popovic, Milica Veljkovic, Dejan Bezbradica, Aleksandar Marinkovic
Summary: This study produced novel lignin-based microspheres and explored the immobilization of laccase on these microspheres. The results showed that under specific conditions, the immobilization process achieved good immobilization yield and catalytic activity, and the LMS-DEGDMA-laccase preparation showed high efficiency in decolorization.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Review
Biochemistry & Molecular Biology
F. Rafiee, M. Rezaee
Summary: Immobilized enzymes, particularly lipase on chitosan support, have attracted significant attention due to their advantages in industry and biochemistry. This review highlights the recent studies on lipase immobilization and discusses the potential solutions for current limitations.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Parsa Hariri, Hossein Jafari-Nodoushan, Somayeh Mojtabavi, Nastaran Hadizadeh, Farnoosh Rezayaraghi, Mohammad Ali Faramarzi
Summary: In this study, casein@CoFe2O4 was successfully synthesized and used to immobilize laccase. The immobilized enzyme showed improved stability and reusability compared to the free enzyme. The constructed biocatalyst effectively degraded crystal violet, reducing its antimicrobial activity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Environmental Sciences
Jamal M. Khaled, Sami A. Alyahya, Rajivgandhi Govindan, Chenthis Kanisha Chelliah, Muthuchamy Maruthupandy, Naiyf S. Alharbi, Shine Kadaikunnan, Reya Issac, Sevanan Murugan, Wen-Jun Li
Summary: Bacterial laccases have gained increasing interest in industry and environmental applications, particularly in the decolorization of azo dyes. This study demonstrates that laccases from B. Cereus and P. parafulva show high potential for decolorization and can be immobilized to improve their activity.
ENVIRONMENTAL RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Agnieszka Kolodziejczak-Radzimska, Michal Bielejewski, Joanna Zembrzuska, Filip Ciesielczyk, Teofil Jesionowski, Long D. Nghiem
Summary: This study developed a zirconia-fucoidan (ZrF) material as a substrate for immobilizing laccase, which showed a high immobilization efficiency and improved enzymatic activity compared to free laccase. The ZrF-laccase system demonstrated effective degradation of tartrazine, achieving 95% decolorization in five cycles. The proposed methodology combining ZrF, fucoidan, and laccase provides a promising approach for the removal of emerging pollutants from the environment.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2023)
Article
Multidisciplinary Sciences
Hyeryeong Lee, Eun Mi Lee, Stacy Simai Reginald, In Seop Chang
Summary: This study compared the effects of different gold-binding peptides fused to enzymes on electrocatalytic activity, bioactivity, and material-binding behaviors, demonstrating the vital role of GBP type in the design, construction, and optimization of enzyme-modified electrodes for efficient interfacial DET in DET-based enzyme-electrode systems.
Article
Polymer Science
Nadya Vasileva Dencheva, Joana Filipa Barros Braz, Zlatan Zlatev Denchev
Summary: This study presents a facile method for synthesizing pure, hybrid, and copolymeric microparticulate materials for additive manufacturing via anionic ring-opening polymerization in solution. The synthesized materials demonstrate different characteristics based on reaction time and temperature, with the potential for use in additive manufacturing processing. The materials show promising mechanical properties, indicating their suitability for applications in the additive manufacturing industry.
JOURNAL OF APPLIED POLYMER SCIENCE
(2022)
Article
Materials Science, Multidisciplinary
Filipa M. Oliveira, Luis Martins, Nadya Dencheva, Tiberio A. Ezquerra, Zlatan Z. Denchev
Summary: This study reports a method based on reactive microencapsulation for preparing thermoplastic composites that can simultaneously provide EMI shielding, easy processability, and cost-effectiveness. Metal particles dispersed in a conductive CNT network were found to contribute to effective attenuation of electromagnetic waves.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Multidisciplinary Sciences
Shu-Jen Wang, Michael Sawatzki, Ghader Darbandy, Felix Talnack, Jorn Vahland, Marc Malfois, Alexander Kloes, Stefan Mannsfeld, Hans Kleemann, Karl Leo
Summary: Devices made using thin-film semiconductors, especially organic semiconductors, have gained significant attention due to their new application possibilities. In this study, we present organic bipolar transistors with outstanding device performance, achieved through a previously undescribed vertical architecture and highly crystalline organic rubrene thin films. These transistors exhibit high differential amplification and superior high-frequency performance, providing insight into the minority carrier diffusion length in organic semiconductors.
Article
Biochemistry & Molecular Biology
Joana F. Braz, Nadya V. Dencheva, Marc Malfois, Zlatan Z. Denchev
Summary: This study focuses on the synthesis and characterization of novel bienzyme polymer-assisted nanoflower complexes (PANF) and their effectiveness as cascade biocatalysts. Highly porous polyamide 6 microparticles (PA6 MP) are synthesized and used as carriers for hybrid bienzyme assemblies comprising glucose oxidase (GOx) and horseradish peroxidase (HRP). The PANF complexes demonstrate improved catalytic activity, enhanced kinetic parameters, thermal stability, optimal pH performance, and storage stability, making them promising as biocatalysts.
Article
Nanoscience & Nanotechnology
Puskal Kunwar, Bianca Louise Andrada, Arun Poudel, Zheng Xiong, Ujjwal Aryal, Zachary J. J. Geffert, Sajag Poudel, Daniel Fougnier, Ivan Gitsov, Pranav Soman
Summary: We introduce a new method to shape double-network (DN) hydrogels into customized 3D structures with superior mechanical properties. This method involves optimizing a prepolymer formulation containing acrylamide and κ-carrageenan to create tough DN hydrogels. We use a TOPS system to photopolymerize the primary acrylamide network, while cooling generates the secondary κ-carrageenan network, resulting in highly resilient DN hydrogel structures. These 3D structures exhibit high stress and strain under tension, as well as high compression stress and strain, with excellent recovery rates. Furthermore, we demonstrate the potential of this technology by printing a flexible axicon lens that can dynamically tune a Bessel beam.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Dariya Getya, Alec Lucas, Ivan Gitsov
Summary: Pollution with organic dyes is a typical environmental problem related to industrial wastewater. This paper reports the synthesis of novel fortified hydrogels that can bind and remove organic dyes from aqueous solutions, offering potential for environmental remediation. These hydrophilic conetworks consist of chemically modified poly(ethylene glycol) (PEG-m) and multifunctional cellulose macromonomers (cellu-mers).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Andreea Nadaban, Jannik Rousel, Dounia El Yachioui, Gerrit S. Gooris, Charlotte M. Beddoes, Robert M. Dalgliesh, Marc Malfois, Robert Rissmann, Joke A. Bouwstra
Summary: The lipids in the uppermost layer of the skin, the stratum corneum (SC), play a vital role in the skin barrier function. Inflammatory skin diseases alter the composition of these lipids, particularly the molar ratio between CER NS and CER NP, which is associated with impaired skin barrier function. This study investigated the impact of varying the CER NS:CER NP ratios on lipid organization, lipid arrangement, and barrier functionality in SC lipid model systems.
JOURNAL OF LIPID RESEARCH
(2023)
Article
Polymer Science
Federico Di Sacco, Eduardo Solano, Marc Malfois, Jingbo Wang, Markus Gahleitner, Roberto Pantani, Giuseppe Portale
Summary: Understanding the crystallization behavior of isotactic polypropylene during the cast film extrusion process is crucial for achieving desired material performances. Real-time X-ray characterization was performed for the first time ever, providing excellent quality data on the phase crystallinity evolution. Three crystallization zones were observed: an amorphous zone, a frostline zone, and a high-crystallinity zone. The experimental data was matched with simulated data, showing good agreement in terms of crystalline development over the investigated time scale.
Article
Multidisciplinary Sciences
Mattia Fontana, Mark Roosjen, Isidro Crespo Garcia, Willy van den Berg, Marc Malfois, Roeland Boer, Dolf Weijers, Johannes Hohlbein
Summary: The signaling molecule auxin coordinates various growth and development processes in plants by modulating gene expression. Transcriptional response is mediated by the family of auxin response factors (ARF), which recognize a DNA motif and can form homodimers. In this study, the affinity and kinetics of ARFs' interaction with an auxin-responsive element were investigated using a single-molecule Forster resonance energy transfer (smFRET) DNA binding assay. The results showed that both the DNA-binding domain (DBD) and the PB1 domain of ARF contribute to DNA binding, and ARF dimer stability plays a key role in defining binding affinity and kinetics.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Polymer Science
Xin Liu, F. Max Yavitt, Ivan Gitsov
Summary: This study focuses on the synthesis of amphiphilic linear-dendritic block copolymers and their self-assembly in water to form supramolecular nanoreactors. The block copolymers were successfully synthesized through copper(I)-catalyzed alkyne-azide cycloaddition. The self-assembly of the copolymers was characterized, and the micelles formed showed efficient chelating groups for transition metals. The catalytic activity of the supramolecular linear-dendritic/Pd complexes in Suzuki-Miyaura reactions was tested and showed high yields under green conditions.
Article
Polymer Science
Joana F. Braz, Nadya V. Dencheva, Shafagh D. Tohidi, Zlatan Z. Denchev
Summary: Non-invasive glucose determination offers significant advantages in health monitoring, allowing affordable, sensitive, specific, rapid, and equipment-free point-of-care testing. This study presents a colorimetric biosensor for detecting glucose in human urine, using highly porous polyamide microparticles as a support for glucose oxidase (GOx) and horseradish peroxidase (HRP). The biosensor exhibited a linear correlation between color difference and glucose concentration, with a detection limit of 30.7 μM in urine and stable performance even after 30 days of storage. The unique feature of this biosensor is its ability for multiple consecutive uses without significant deterioration.
Article
Materials Science, Multidisciplinary
Xin Liu, Zhiyue Gao, Die Wang, Fengjie Yu, Baoshuai Du, Ivan Gitsov
Summary: Organic coatings are an effective way to improve the corrosion resistance of metals. This study presents a feasible method of preparing modified halloysite nanotubes and using them as multifunctional anticorrosion additives for environmentally friendly coatings. The incorporation of a corrosion inhibitor enhances the corrosion resistance and self-healing potential of the coatings.