Article
Behavioral Sciences
Xin Zheng, Jianhui Zhu, Jiaxin Liu, Hong Wang, Yumei Qin, Peihua Jiang, Li Xiao, Tao Gong, Yuqing Li, Xian Peng, Xin Xu, Lei Cheng, Liquan Huang, Qianming Chen, Xuedong Zhou, Robert F. Margolskee
Summary: A novel splicing variant of the sweet taste receptor gene Tas1r2 was identified in mouse taste buds, which diminishes sweet taste responses in vitro and in vivo by reducing the function of sweet taste receptors.
Article
Oncology
Yasushi Mochizuki, Ryo Funayama, Matsuyuki Shirota, Yuna Kikukawa, Masahiro Ohira, Hideaki Karasawa, Minoru Kobayashi, Shinobu Ohnuma, Michiaki Unno, Keiko Nakayama
Summary: This study showed differential splicing of microexons in colorectal cancer, with 21 genes enriched in gene ontology terms related to cell adhesion and migration. RNA interference experiments identified RBFOX2 and PTBP1 as splicing regulators in CRC cells. Changes in microexon splicing patterns were associated with CRC metastasis, likely influenced by altered expression of RBFOX2 and PTBP1.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Cell Biology
Yanfei Wang, Cuiqiao Zhang, Wu Peng, Haibo Du, Yuehui Xi, Zhigang Xu
Summary: This study further explored the role of RBM24 in hearing and balance through conditional knockout mice and found that the loss of RBM24 results in severe deficits. It was observed that the development of hair cells was significantly affected, leading to degeneration and disorganization of the hair bundles. RNAseq analysis also identified several genes whose mRNA stability or pre-mRNA alternative splicing is affected by RBM24 knockout.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Serena Huei-An Lu, Kang-Zheng Lee, Paul Wei-Che Hsu, Liang-Yu Su, Yu-Chen Yeh, Chien-Yuan Pan, Su-Yi Tsai
Summary: Splicing variants of sarcomeric proteins play key roles in myofibrillogenesis. RBM24 acts as a master regulator to modulate the temporal dynamics of core myofibrillogenesis genes, thereby orchestrating sarcomere organization.
CIRCULATION RESEARCH
(2022)
Article
Cell Biology
Chungen Lan, Huikun Zhang, Kezhen Wang, Xiaoli Liu, Yawen Zhao, Zhifang Guo, Ning Zhang, Yongxia Zhou, Manzhi Gao, Feng Gu, Yongjie Ma
Summary: The study demonstrates that PTBP1 modulates the alternative splicing of ITSN1, promoting glioma proliferation and motility by up-regulating the ratio of ITSN1-S/ITSN1-L, suggesting that PTBP1 may be a promising therapeutic target for gliomas.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Longqing Zheng, Huijun Yuan, Mengkai Zhang, Cuicui Wang, Xuemin Cai, Jing Liu, Xiu Qin Xu
Summary: Tissue-specific alternative splicing is regulated by Rbm24 in the inner ear, impacting hearing and motor coordination. This study reveals that Rbm24 directly controls the splicing of multiple genes, including those crucial for stereocilia integrity and hair cell survival.
Article
Multidisciplinary Sciences
Zhentao He, Qianhua Ni, Xichun Li, Mingyu Zhao, Qingguo Mo, Yongsheng Duo
Summary: Aberrant alternative splicing of PTBP1 is associated with poor prognosis and increased metastatic potential in hepatocellular carcinoma (HCC). Elevated PTBP1 expression promotes migration and invasion of HCC cells, while knockdown of PTBP1 inhibits these processes. PTBP1 regulates the alternative splicing of NUMB exon 9 and has potential as a prognostic indicator in HCC.
Article
Cell Biology
Riho Komuro, Yuka Honda, Motoaki Yanaizu, Masami Nagahama, Yoshihiro Kino
Summary: Genetic variations of CD33, specifically the rs12459419 polymorphism on exon 2, affect its alternative splicing and have been associated with the risk of Alzheimer's disease. The minor allele promotes skipping of exon 2, resulting in a shorter CD33 isoform lacking the ligand-binding domain and reduced suppressive signaling on microglial activity. HNRNPA1 was found to promote exon 2 skipping, but simultaneous knockdown of HNRNPA1, HNRNPA2B1, and HNRNPA3 increased exon 2 inclusion, suggesting functional redundancy among these proteins.
Article
Oncology
Arpankumar Choksi, Apoorva Parulekar, Richa Pant, Vibhuti Kumar Shah, Ramakrishna Nimma, Priyanka Firmal, Smriti Singh, Gopal C. Kundu, Sanjeev Shukla, Samit Chattopadhyay
Summary: The study demonstrates that SMAR1 inhibits the Warburg effect by promoting PKM1 expression over PKM2 in breast cancer cells. It also shows that SMAR1 deacetylates PTBP1, leading to reduced enrichment of PTBP1 on PKM pre-mRNA and suppression of cancer cell metabolism and tumorigenesis. Additionally, SMAR1 suppresses tumor formation in vivo in a PKM2-dependent manner.
CANCER & METABOLISM
(2021)
Article
Cell Biology
Yuka Ikeda-Iwabu, Yoshiaki Taniyama, Naruto Katsuragi, Fumihiro Sanada, Nobutaka Koibuchi, Kana Shibata, Kenzo Shimazu, Hiromi Rakugi, Ryuichi Morishita
Summary: The study found that POSTN in cancer cells can be detected using an Ex17 antibody but not an Ex12 antibody. A short fragment of POSTN with exon 17 was identified in the fraction secreted from fibroblasts. POSTN with exon 17 specifically bound to wnt3a, and this binding was inhibited by the Ex17 antibody.
Article
Biotechnology & Applied Microbiology
A. Gallo, D. Dolfini, A. Bernardini, N. Gnesutta, R. Mantovani
Summary: NF-YA, the regulatory subunit of the trimeric CCAAT-binding transcription factor NF-Y, has multiple alternative spliced isoforms, and NF-YAx and NF-YAg are enriched in Aves and play a major role in early chicken embryo development. In addition, the absence of exon-5 in NF-YAg leads to a global reduction of beta-stranded elements, while removal of the disordered exon-3 sequence has limited effects on TAD architecture.
Article
Biochemistry & Molecular Biology
Francisco Carranza, Hossein Shenasa, Klemens J. Hertel
Summary: Alternative splicing is a highly combinatorial mechanism that allows higher eukaryotes to increase mRNA diversity. The selection of alternative splice sites is influenced by sequence, cis-regulatory elements, exon and intron length, and RNA secondary structures. The study showed that the mode of splice site recognition, either intron or exon definition, plays a role in alternative splicing decisions.
Article
Biochemistry & Molecular Biology
Amy M. Cooper, Curtis A. Nutter, Muge N. Kuyumcu-Martinez, Casey W. Wright
Summary: The study found that the upregulation of RNA-binding protein RBFOX2 is correlated with the increase in ARNT isoform 1 in human lymphoid malignancies, and suppression of RBFOX2 significantly reduces ARNT isoform 1 levels and cell growth.
MOLECULAR AND CELLULAR BIOLOGY
(2022)
Article
Immunology
Guangfeng Geng, Changlu Xu, Nan Peng, Yue Li, Jinhua Liu, Jing Wu, Jing Liang, Yushan Zhu, Lihong Shi
Summary: PTBP1 in dendritic cells plays a crucial role in regulating MHC II expression, antitumour immunity, and asthma exacerbation, highlighting its potential as a therapeutic target for cancer treatment.
Article
Virology
Katherine E. Havranek, Luke Adam White, Thomas C. Bisom, Jean-Marc Lanchy, J. Stephen Lodmell
Summary: Recent studies have shown that during RVFV infection, the host splicing landscape changes, with the selective splicing of RIOK3 playing a crucial role in the interferon signaling pathway.