期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 146, 期 -, 页码 107-113出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2016.05.043
关键词
Bovine serum albumin; Chitosan; Self-assembly; Nanogels; Doxorubicin hydrochloride; Anticancer
资金
- National Natural Science Foundation of China [31301426]
- Specialized Research Fund for the Doctoral Program of Higher Education [20130146120019]
- Natural Science Foundation of Hubei Province of China [2013CFB198]
In this study, bovine serum albumin (BSA) and chitosan (CS) were used to prepare BSA-CS nanogels by a simple green self-assembly technique. Then the nanogels were successfully used to entrap doxorubicin hydrochloride (DOX) with an entrapment ratio of 46.3%, aiming to realize the slow-release effect and lower the cytotoxicity of DOX. The IC50 values of DOX-loaded BSA-CS (DOX-BSA-CS) and free DOX obtained by MTT assay in SGC7901 cells were 0.22 and 0.05 mu g/mL, respectively. The cytotoxicity of DOX significantly decreased within 24h after encapsulation by the nanogels, indicating that the loaded drug could slowly release within 24h and the BSA-CS was a good slow release system. The cellular uptake experiments indicated DOX-BSA-CS diffused faster into the cancer cell than the bare drug. The flow cytometry and TUNEL assay proved DOX-BSA-CS could induce a larger apoptosis proportion of gastric cancer cells 7901 than the bare drug and it is promising to be used for curing gastric cancer. (C) 2016 Published by Elsevier B.V.
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