期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 143, 期 -, 页码 186-193出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2016.03.035
关键词
Urocanic acid; Chitosan; Nanoparticle; Anti-inflammation; CD98 siRNA; Macrophage
资金
- Department of Veterans Affairs
- National Institutes of Health of Diabetes and Digestive and Kidney [RO1-DK-071594]
- National Natural Science Foundation of China [51503172, 81571807]
- Fundamental Research Funds for the Central Universities [SWU114086, XDJK2015C067]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars (State Education Ministry)
- Crohn's & Colitis Foundation of America
CD98 plays an important role in the development and progression of inflammation. Here, CD98 siRNA (siCD98) was complexed with urocanic acid-modified chitosan (UAC) to form nanoparticles (NPs), which were transfected into Raw 264.7 macrophages in an effort to convey anti-inflammatory effects. Characterization showed that the generated NPs had a desirable particle size (156.0-247.1 nm), a slightly positive zeta potential (15.8-17.5 mV), and no apparent cytotoxicity against Raw 264.7 macrophages and colon-26 cells compared to control NPs fabricated by Oligofectamine (OF) and siRNA. Cellular uptake experiments demonstrated that macrophages exhibited a time-dependent accumulation profile of UAC/siRNA NPs. Further in vitro gene silencing experiments revealed that UAC/siCD98 NPs with a weight ratio of 60:1 yielded the most efficient knockdowns of CD98 and the pro-inflammatory cytokine, TNF-alpha. Indeed, the RNAi efficiency obtained with our NPs was even higher than that of the positive control OF/siCD98 NPs. These results suggest that UAC/siCD98 NPs might be a safe, efficient and promising candidate for the treatment of inflammatory disease. (C) 2016 Elsevier B.V. All rights reserved.
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