Review
Plant Sciences
Mohamed Abdelrahman, Zheng Wei, Jai S. S. Rohila, Kaijun Zhao
Summary: Multiplex genome-editing technologies, especially CRISPR/Cas-based tools, have revolutionized plant molecular biology by enabling precise editing of multiple loci or genes, thus advancing crop-breeding methods.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemical Research Methods
Stefan J. Tekel, Nicholas Brookhouser, Kylie Standage-Beier, Xiao Wang, David A. Brafman
Summary: The introduction of transient reporters of editing enrichment (TREE) has enabled highly efficient single-base editing of human cells using a transient episomal fluorescent reporter, allowing for rapid generation of clonal editing efficiencies exceeding 80% in biallelic or multiplexed edited isogenic human pluripotent stem cell lines within approximately 3-4 weeks.
Review
Agriculture, Dairy & Animal Science
Julia Popova, Victoria Bets, Elena Kozhevnikova
Summary: Genome editing has practical applications in farm animals, improving production traits, economic value, and disease resistance. It also has potential in biomedical research and drug production, as well as xenograft donors. Recent advancements in site-specific nucleases and embryological delivery methods have revolutionized transgenesis, providing efficient and reliable tools for genome engineering in agriculture.
Review
Biochemistry & Molecular Biology
Yulin Mu, Chengxiao Zhang, Taihua Li, Feng-Jie Jin, Yun-Ju Sung, Hee-Mock Oh, Hyung-Gwan Lee, Long Jin
Summary: Lactobacillus, important in food production and probiotics, can benefit from CRISPR/Cas9-based genome editing to improve strain efficiency and achieve traceless genome modification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Emilia Bottero, Cristina Gomez, Margarita Stritzler, Hiromi Tajima, Romina Frare, Cecilia Pascuan, Eduardo Blumwald, Nicolas Ayub, Gabriela Soto
Summary: This study presents the first report of base editing in alfalfa, demonstrating edited alfalfa varieties that are tolerant to both sulfonylurea- and imidazolinone-type herbicides.
PLANT CELL REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Magdalena Klimek-Chodacka, Miron Gieniec, Rafal Baranski
Summary: The study demonstrates an efficient, recombinant DNA-free, multiplex gene-editing method using gRNA:Cas9 ribonucleoprotein (RNP) complexes delivered directly to plant protoplasts. By forming three RNPs in vitro and delivering them to protoplasts, various editing effects were achieved with high efficiency, showcasing the potential of this method.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Xueli Zhang, Chaohui Zhang, Caijiao Liang, Bizhou Li, Fanmei Meng, Yuncan Ai
Summary: Bacteriophages, the most abundant organisms in the biosphere, have been sequenced extensively. However, the study of bacteriophage functional genomics has been hindered by a lack of effective research methods. This study designed a phage genome editing platform based on the CRISPR-Cas9 system, and successfully achieved gene editing in Vibrio natriegens phage TT4P2. This platform has the potential to advance research on phage gene diversity and accelerate the development of phage synthetic biology and nanotechnology.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Fang Yan, Jingwen Wang, Sujie Zhang, Zhenwan Lu, Shaofang Li, Zhiyuan Ji, Congfeng Song, Gongyou Chen, Jin Xu, Jie Feng, Xueping Zhou, Huanbin Zhou
Summary: CRISPR/Cas-based genome editing technology has important applications in bacteria, allowing efficient gene knockout and other editing operations. We have developed a targeted genome editing method for Xanthomonas oryzae pv. oryzae (Xoo), a major pathogen of rice, using heterologous CRISPR/Cas12a from Francisella novicida and NHEJ proteins from Mycobacterium tuberculosis. This method enables efficient gene knockout, gene cluster deletion, and plasmid curing in Xoo strains. Moreover, we have shown that these strategies are also applicable for targeted genome editing in another bacterial plant pathogen, Pseudomonas syringae pv. tomato (Pst). We believe that our bacterial genome editing method will greatly expand CRISPR studies on microorganisms and enhance our understanding of the physiology and pathogenesis of Xoo.
Review
Pharmacology & Pharmacy
Siwei Chen, Deng Chen, Bin Liu, Hidde J. Haisma
Summary: CRISPR/Cas9-mediated genome engineering has extensive applications in basic biology, biotechnology, and medicine. However, the low gene modification efficiency and uncontrollable prolonged Cas9 activity hinder its further use. Researchers have explored small molecules with clinical potential to precisely modulate CRISPR/Cas9 genome editing activity.
DRUG DISCOVERY TODAY
(2022)
Article
Biotechnology & Applied Microbiology
Tezera W. Wolabu, Kashif Mahmood, Ivone Torres Jerez, Lili Cong, Jianfei Yun, Michael Udvardi, Million Tadege, Zengyu Wang, Jiangqi Wen
Summary: In this study, the flowering time in alfalfa was delayed by using CRISPR/Cas9-mediated mutagenesis of the FT gene. The mutant lines showed increased forage biomass yield and improved quality.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Min Ho Lee, Jae Il Shin, Jae Won Yang, Keum Hwa Lee, Do Hyeon Cha, Jun Beom Hong, Yeoeun Park, Eugene Choi, Kalthoum Tizaoui, Ai Koyanagi, Louis Jacob, Seoyeon Park, Ji Hong Kim, Lee Smith
Summary: This review highlights the current and potential use of CRISPR-Cas9 in the management of autoimmune diseases. Several studies have shown that CRISPR-Cas9 can be utilized for immunomodulation, reducing cholesterol, and treating rare diseases. However, there is limited research on the treatment of autoimmune diseases using CRISPR-Cas9.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Arvind Sharma, Michael N. Pham, Jeremiah B. Reyes, Randeep Chana, Won C. Yim, Chan C. Heu, Donghun Kim, Duverney Chaverra-Rodriguez, Jason L. Rasgon, Robert A. Harrell, Andrew B. Nuss, Monika Gulia-Nuss
Summary: Ticks have the ability to transmit various debilitating pathogens, but research on ticks has been lacking compared to other arthropod vectors due to challenges in applying genetic and molecular tools. This study reports a successful embryo injection protocol for the black-legged tick and demonstrates the use of CRISPR-Cas9 for genome editing. The results provide innovative tools for tick research.
Article
Biotechnology & Applied Microbiology
Kristina J. Tatiossian, Robert D. E. Clark, Chun Huang, Matthew E. Thornton, Brendan H. Grubbs, Paula M. Cannon
Summary: This study found that CRISPR-Cas9 indel signatures can be used to identify gRNAs that maximize HDR outcomes, with the frequency of deletions resulting from MMEJ repair of at least 3 base pairs better predicts HDR frequency. Existing tools can be repurposed to identify gRNAs that promote HDR.
Article
Plant Sciences
Syed Inzimam Ul Haq, Dianfeng Zheng, Naijie Feng, Xingyu Jiang, Feng Qiao, Jin-Sheng He, Quan-Sheng Qiu
Summary: This review summarizes the advances of CRISPR/Cas9 technology in plant genome editing and discusses its applications in forage breeding, as well as the potential and challenges.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Chemistry, Physical
Juhee Lee, Yoo Kyung Kang, Eonju Oh, Juhee Jeong, San Hae Im, Duk Ki Kim, Haeshin Lee, Sang-Gyu Kim, Keehoon Jung, Hyun Jung Chung
Summary: The study presents a cancer gene therapy strategy based on NanoRNP that efficiently blocks the PD-L1 immune checkpoint and induces an antitumor effect in vivo without the need for combination therapy. In vivo results demonstrate that NanoRNP can induce indels in target cells at high frequencies, significantly suppressing tumor growth.
CHEMISTRY OF MATERIALS
(2022)