期刊
TOXINS
卷 12, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/toxins12060379
关键词
Hymenoptera venom allergy; Polybia paulista; CD4(+)T cells
资金
- Council of Technological and Scientific Development (CNPq)-Brazil [455422/2014-1]
- Sao Paulo Research Foundation (FAPESP) [2019/00729-7, 2014/13936-7]
- CAPES-DS (Coordination for the Improvement of Higher Education Personnel)
- Postgraduate Program in Internal Medicine at the State University of Campinas (UNICAMP)
- FAPESP [2017/22405-3, 2018/24834-1]
- Campinas-SP
Insect venom can cause systemic allergic reactions, including anaphylaxis. Improvements in diagnosis and venom immunotherapy (VIT) are based on a better understanding of an immunological response triggered by venom allergens. Previously, we demonstrated that the recombinant phospholipase A1 (rPoly p 1) fromPolybia paulistawasp venom induces specific IgE and IgG antibodies in sensitized mice, which recognized the native allergen. Here, we addressed the T cell immune response of rPoly p 1-sensitized BALB/c mice. Cultures of splenocytes were stimulated withPolybia paulistavenom extract and the proliferation of CD8(+)and CD4(+)T cells and the frequency of T regulatory cells (Tregs) populations were assessed by flow cytometry. Cytokines were quantified in cell culture supernatants in ELISA assays. The in vitro stimulation of T cells from sensitized mice induces a significant proliferation of CD4(+)T cells, but not of CD8(+)T cells. The cytokine pattern showed a high concentration of IFN-gamma and IL-6, and no significant differences to IL-4, IL-1 beta and TGF-beta 1 production. In addition, the rPoly p 1 group showed a pronounced expansion of CD4(+)CD25(+)FoxP3(+)and CD4(+)CD25(-)FoxP3(+)Tregs. rPoly p 1 sensitization induces a Th1/Treg profile in CD4(+)T cell subset, suggesting its potential use in wasp venom immunotherapy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据