期刊
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
卷 17, 期 13, 页码 -出版社
MDPI
DOI: 10.3390/ijerph17134723
关键词
chlorpyrifos residue; ic-ELISA; GC-FPD; northern Thailand
资金
- Thailand Research Fund, Chiang Mai University [TRG6080007]
- NIEHS/Superfund Research Program Biomarkers of exposure to hazardous substances [P42 ES004699]
Chlorpyrifos is an organophosphate pesticide that is wildly used among farmers for crop protection. However, there are concerns regarding its contamination in the environment and food chain. In the present study, an in-house indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) specific for detecting chlorpyrifos is developed and validated against gas chromatography-flame photometric detection (GC-FPD) as the conventional method. The developed ic-ELISA was used for detecting chlorpyrifos residue in vegetable samples. The developed ic-ELISA showed good sensitivity to chlorpyrifos at an IC(50)of 0.80 mu g/kg, with low cross-reactivity to other organophosphate pesticides. The 160 samples were collected from local markets located in the Chiang Rai, Chiang Mai, and Nan provinces in northern Thailand. The positive rate of chlorpyrifos residues in the vegetable samples was 33.8%, with the highest levels found in cucumbers, coriander, and morning glory, at 275, 145, and 35.3 mu g/kg, respectively. The highest median levels of chlorpyrifos found in the detected samples were Chinese cabbage (332 mu g/kg), cucumber (146.3 mu g/kg) and Chinese Kale (26.95 mu g/kg). The developed ic-ELISA is suitable for the rapid quantitation of chlorpyrifos residues.
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