Article
Infectious Diseases
Bo Yang, Zhengwang Shi, Yuan Ma, Lijuan Wang, Liyan Cao, Juncong Luo, Ying Wan, Rui Song, Yiyong Yan, Kehu Yuan, Hong Tian, Haixue Zheng
Summary: A method for the convenient, portable, low cost, highly sensitive and specific detection of African swine fever virus (ASFV) was established using a combined LAMP-CRISPR assay. The detection limit reached 7 copies/microliter of p72 gene per reaction with no cross-reactivity with other porcine viruses. The performance of this method showed good consistency with real-time qPCR tests, demonstrating a great potential for on-site monitoring of ASFV in the field.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Chemistry, Analytical
Yanju Chen, Siwenjie Qian, Jian Wu, Xiaoping Zhong, Meixia Wang, Jin-song He, Xiaoping Yu
Summary: In this study, a microchip-assisted detection platform was proposed for the detection of African swine fever virus (ASFV). The liquid transfer between different reaction chambers of the microchip is driven by the gravity of liquid solutions, eliminating the need for complex liquid transfer and instruments. The platform has the advantages of low cost, high efficiency, and easy operation, making it promising for popularization in low-resourced areas.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biotechnology & Applied Microbiology
Gaihua Cao, Yifan Xiong, Fuping Nie, Xiaolong Chen, Lan Peng, Yingguo Li, Mei Yang, Danqun Huo, Changjun Hou
Summary: A fluorescent biosensor and lateral flow assay strip based on PCR and CRISPR/Cas12a system were developed for the detection of African swine fever virus (ASFV). The biosensor exhibited high sensitivity and specificity, and showed comparable accuracy to traditional qPCR in point-of-care testing.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Veterinary Sciences
Chao Qin, Jiajia Liu, Wenqi Zhu, Muchu Zeng, Ke Xu, Jinmei Ding, Hao Zhou, Jianshen Zhu, Yuqing Ke, Lai Yan Li, Gaoyuan Sheng, Zhuoru Li, Huaixi Luo, Shengyao Jiang, Kangchun Chen, Xianting Ding, He Meng
Summary: African swine fever virus (ASFV) is a major cause of worldwide agricultural loss, and conventional detection methods are time-consuming and instrument-dependent. In this study, a one-pot visual detection system for ASFV using CRISPR/Cas12a technology combined with LAMP or RPA was developed. The system allows rapid, sensitive, and specific detection of ASFV and can be observed by the naked eye, making it suitable for point-of-care testing.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Chemistry, Analytical
Huimin Wang, Ailing Su, Chengxin Bao, Chongyang Liang, Weiqing Xu, Jingjing Chang, Shuping Xu
Summary: A surface-enhanced Raman scattering (SERS) strategy combined with a CRISPR/Cas12a system is designed for amplification-free gene detection of African swine fever virus (ASFV). The method achieves high sensitivity and selectivity in liquid-phase detection, and is mainly used for on-site inspection and quick testing of gene samples.
Article
Chemistry, Analytical
Guobin Mao, Xing Luo, Silu Ye, Xun Wang, Jin He, Jilie Kong, Junbiao Dai, Wen Yin, Yingxin Ma
Summary: In this study, a magnetic separation method based on recombinase polymerase amplification (RPA)-assisted clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a was developed for dual-mode analysis of African swine fever virus (ASFV) genes. This method combines the advantages of colorimetry and fluorescence, enabling sensitive and low-cost detection of viral nucleic acid.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Siwenjie Qian, Yanju Chen, Cheng Peng, Xiaofu Wang, Hui Wu, Yang Che, Huanying Wang, Junfeng Xu, Jian Wu
Summary: This article introduces a simple and cost-effective method for detecting African swine fever virus (ASFV). The method utilizes filter paper to purify nucleic acids from swine blood and employs CRISPR/Cas12a-mediated loop-mediated isothermal amplification (LAMP) reaction to detect ASFV. The method does not require complex laboratory conditions, and has the advantages of rapid and visualized detection, making it suitable for on-site application.
Article
Agriculture, Multidisciplinary
Wang Peng-fei, Wang Ming, Shi Zhi-bin, Sun Zhen-zhao, Wei Li-li, Liu Zai-si, Wang Shi-da, He Xi-jun, Wang Jing-fei
Summary: This study developed an indirect ELISA assay using a recombinant pB602L protein as the antigen, which showed no cross-reaction with the anti-sera against common pig pathogens in China. The coincidence of this assay with a commercial ELISA kit was 95%. Additionally, the assay was used to detect antibody responses in vaccinated pigs against a specific ASFV strain.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Multidisciplinary Sciences
Menglu Hu, Zhiqiang Qiu, Zirong Bi, Tian Tian, Yongzhong Jiang, Xiaoming Zhou
Summary: This article proposes a CRISPR diagnostic method based on optochemical control, which addresses the issues of contamination risks and insufficient sensitivity in nucleic acid amplification-based diagnostics. The method achieves comparable detection sensitivity and specificity to PCR, and avoids contamination risks through photocontrol.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Yong Chen, Yixin Mei, Xingyu Jiang
Summary: This study presents a novel SNP detection method using the CRISPR system to improve universality, sensitivity, and specificity, successfully applied in clinical blood samples. This genotyping method can rapidly detect multiple genotypes in a short period of time.
Article
Spectroscopy
Xin Wang, Yuxin Qin, Yong Huang, Kun Hu, Shulin Zhao, Jianniao Tian
Summary: In this study, miRNA detection was achieved by coupling the CRISPR-Cas system with strand displacement amplification. The method showed high sensitivity and selectivity, making it suitable for miRNA biomarker diagnosis.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Microbiology
Alexey D. Sereda, Sanzhi Namsrayn, Vladimir M. Balyshev, Mikhail E. Vlasov, Irina P. Sindryakova, Galina Koltsova, Denis V. Kolbasov
Summary: The extreme genetic and immunobiological heterogeneity of African swine fever virus poses challenges to vaccine development. This article provides a comprehensive description of biological techniques for the classification of ASFV by seroimmunotypes, emphasizing the importance of conducting immunological tests on pigs to ensure accuracy.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Analytical
Chengfei Li, Zhong Zou, Changjie Lv, Ya Zhao, Pengfei Han, Xiaomei Sun, Meilin Jin
Summary: In this study, a reliable and sensitive suspension microarray technology was developed for ASFV antibody detection. The newly developed method showed a 16-fold improvement in detection sensitivity compared to commercial ELISA kits and demonstrated a 97% consistency rate. This method has great potential for ASF epidemic surveillance and control.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Luping Deng, Jinyao Du, Xu Hun
Summary: A nanoprobe photoelectrochemical (PEC) assay combined with CRISPR/Cas12a was developed for nucleic acid detection. Ternary heterojunctions, nanoflower MoS2/WS2/g-C3N4, were synthesized by hydrothermal method. The PEC assay showed high sensitivity and selectivity, and provided a different detection pattern for other nucleic acids.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Jisun Ki, Hee-Kyung Na, Sun Woo Yoon, Van Phan Le, Tae Geol Lee, Eun-Kyung Lim
Summary: In this study, a colorimetric sensing platform based on dual enzymatic amplification was developed for accurate and sensitive detection of African swine fever virus (ASFV). The method combines the CRISPR/Cas12a system and urease enzyme, allowing for rapid and simple diagnosis of zoonotic diseases.
Article
Computer Science, Information Systems
Mengyao Zhang, Liwei Lin, Zhuyun Chen
Summary: This research investigates the structure of a dynamic data management system using blockchain model in E-commerce and proposes a lightweight security scheme for better data management. The experiment shows that the system has better overall performance.
CLUSTER COMPUTING-THE JOURNAL OF NETWORKS SOFTWARE TOOLS AND APPLICATIONS
(2023)
Article
Chemistry, Applied
Yong Zhang, Hao Li, Zhijian Xu, Yulong Shi, Bo Li, Jia Li, Haji Akber Aisa, Yi Zang, Weiliang Zhu
Summary: Three new feruloyl glucuronopyranosyl glycerols were isolated from Eriocaulon buergerianum Koern and identified as potential inhibitors of TGF-beta 1 receptor. Among them, one glycerol (1) exhibited strong inhibitory activity against TGF-beta-induced protein expression in human hepatic stellate LX-2 cells. These findings suggest that the new feruloyl glucuronopyranosyl glycerol 1 has preventive and therapeutic potentials against hepatic fibrosis.
NATURAL PRODUCT RESEARCH
(2023)
Article
Materials Science, Textiles
Qilu Cui, Jiawei Li, Chongwen Yu
Summary: In this study, the extraction and purification of total flavonoids from hemp fiber extracts were investigated using ethyl acetate and three different resins. The results showed that the HPD-500 resin was suitable for purification, with a purity of 8.36% flavonoids obtained. The chemical structure of the flavonoid extracts contained C=C, C-H, C-O, and rutin derivatives. Additionally, the flavonoid extracts enhanced the UV-visible light shielding effect of polyvinyl alcohol (PVA) and exhibited good antioxidant and antibacterial activities.
TEXTILE RESEARCH JOURNAL
(2023)
Article
Materials Science, Ceramics
Jian Zhang, Yuefeng Ma, Jiale Huang, Haoyu Zeng, Zihao Liu, Tengfei Hua, Jiaping Liu, Zhangli Hu
Summary: This study investigates the mechanism of a novel shrinkage-reducing polycarboxylate superplasticizer (SR-PCA) in cementitious materials. The results show that SR-PCA has a mild impact on C-S-H crystallinity and significantly improves the pore structure of C-S-H.
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
(2023)
Article
Polymer Science
Ji Jia, Jiali Li, Si Ma, Zhenwei Zhang, Xiaoming Liu
Summary: In this study, a new covalent organic framework material, COF-JLU82, is designed and synthesized. It exhibits good electrocatalytic activity for the oxygen reduction reaction (ORR), with better performance compared to the previously reported COF-JLU23. This study enriches the types of COF-based ORR electrocatalysts and promotes the development of more highly efficient metal-free ORR electrocatalysts.
MACROMOLECULAR RAPID COMMUNICATIONS
(2023)
Article
Engineering, Biomedical
Ruiyu Bi, Yunlong Zhao, Yuhang Ma, Xiaolong Zhu, Jiawei Li, Bin Wu, Junbin Zang, Jiuzhang Men, Zhidong Zhang, Chenyang Xue
Summary: Pulse position standardization is crucial for the digitization of pulse diagnosis in traditional Chinese medicine. This study presents a pulse wave detection method and pulse position determination method based on gradient pressure. The relationship between the depth of the radial artery and the optimal pulse-taking pressure is discussed. The study proposes criteria for defining pulse position and provides a calculation method. Experimental results validate the feasibility and accuracy of pulse position definition.
BIOMEDICAL SIGNAL PROCESSING AND CONTROL
(2023)
Article
Agricultural Engineering
Jiawei Li, Amjad Ali, Junfeng Su, Tinglin Huang, Zhenyu Zhai, Liang Xu
Summary: This study focused on optimizing the nitrate removal rate under a low carbon-to-nitrogen ratio using a biofilm reactor based on functional carrier and the synergistic effect of strains. Iron-loaded corn cob was used as the functional carrier, promoting cellulose degradation, iron cycling, and collaborative denitrification. The results showed that strain ZY7 promoted the release of carbon source, and the crystallinity of cellulose in the carrier increased significantly. The microbial community exhibited synergistic effects among different strains, enhancing the vitality and metabolic activity of the target microorganisms through interspecific bacterial cooperation.
BIORESOURCE TECHNOLOGY
(2023)
Article
Automation & Control Systems
Daicheng Qin, Junwei Wu, Jiawei Li, Yuncan Ma, Minheng Ye, Dong Tian, Chao Wang, Fang Ji
Summary: Magnesium aluminate spinel materials are widely used as important optical components in harsh environments. However, the high hardness and brittleness of these materials pose challenges in machining and polishing processes. We propose a new method using femtosecond laser-surface-modification assisted MRF to improve the efficiency and quality of spinel MRF, while suppressing the grain effect.
INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY
(2023)
Article
Thermodynamics
Wei Chen, Yingying Wang, Dengjia Wang, Yanfeng Liu, Jiaping Liu
Summary: A novel method based on fractal theory combined with thermoelectric analogy is proposed to accurately predict the effective thermal conductivity of porous building materials. The prediction model outperforms traditional theoretical models and provides a feasible method for obtaining the effective thermal conductivity, which can contribute to understanding the thermal insulation performance and energy conservation of buildings.
INTERNATIONAL JOURNAL OF THERMAL SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Jiayu Liu, Jiaxing Zhang, Liwei Zhang, Yuefei Wang, Hao Wei, Yuhe Shen, Jiwei Min, Xi Rong, Wei Qi, Rongxin Su, Zhimin He
Summary: This study reports the coordination assembly of ferrocene-diphenylalanine (Fc-FF) with divalent copper ions (Cu2+), resulting in the formation of metal-peptide assemblies (MPAs) with hierarchical spiral architectures. The MPAs consist of helically organized nanofibers that resemble logarithmic spirals. These MPAs exhibit hierarchically porous structures with abundant Fc and Cu2+ active sites, displaying significantly higher catalytic activity than natural laccase in decolorization reactions. Furthermore, the temperature and enantiomeric excess (ee) can be controlled to synthesize a series of hierarchical structures for MPAs. Peptide enantiomers with higher ee values self-assemble into highly complex and ordered structures, which exhibit higher surface area and porosity and thus enhanced catalytic activity compared to those assembled by peptides with lower ee values. These findings provide new insights into the crucial role of chirality in directing the self-assembly of biomolecules into highly ordered complex functional structures.
SCIENCE CHINA-CHEMISTRY
(2023)
Article
Environmental Sciences
Xiao Lu, Hongwei Hu, Jiawei Li, Jiangpeng Li, Lijuan Wang, Lili Liu, Yuanyuan Tang
Summary: Through a series of experiments, this study found that the presence of microplastics in sediment can enhance the adsorption capacity of ferrihydrite minerals, particularly for lead with an increase of 36%. The microplastics can also alter the dispersion and surface properties of minerals, thereby affecting the accumulation and transportation of heavy metals at the water-sediment interface.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Robotics
Yuchen Wu, David J. J. Yoon, Keenan Burnett, Soeren Kammel, Yi Chen, Heethesh Vhavle, Timothy D. D. Barfoot
Summary: In this letter, we present a continuous-time lidar-only odometry algorithm that utilizes Doppler velocity measurements from an FMCW lidar to improve performance in geometrically degenerate environments. By applying a continuous-time framework, we accurately estimate the vehicle trajectory and compensate for motion distortion caused by mechanically actuated lidars. Our proposed algorithm outperforms existing methods and demonstrates state-of-the-art performance in both difficult conditions and nominal conditions.
IEEE ROBOTICS AND AUTOMATION LETTERS
(2023)
Article
Environmental Sciences
Wenxuan Fan, Jie Li, Zhiwei Han, Jian Wu, Shuang Zhang, Chuwei Zhang, Jiawei Li
Summary: This study investigated the impact of biomass burning in Southeast Asia on particulate matter concentrations and aerosol properties in downwind areas. A pollution event in Kunming, China on March 31 to April 1, 2019, caused by BB pollutants from Southeast Asia and the India-Myanmar trough, showed significant increases in PM2.5, PM1, and black carbon concentrations. The dominant contributor of BB in Yunnan Province was found to be the Myanmar-Thailand border.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2023)
Article
Computer Science, Artificial Intelligence
Xiang Shi, Yinpeng Liu, Jiawei Liu, Qikai Cheng, Wei Lu
Summary: Scientific papers are crucial for academic communication, but many of them lack in-depth research and present core content ambiguously, hindering the progression of science and technology. To address this challenge, the INTEGrity vERification (INTEGER) task is introduced to help researchers assess the integrity of their papers and verify the clarity of each knowledge unit. A multi-task learning model utilizing Tucker decomposition and span-level attention mechanism is proposed to accurately identify terms and their integrity. Experimental results show the effectiveness of the model.
EXPERT SYSTEMS WITH APPLICATIONS
(2024)
Article
Mathematics, Applied
Yajie Wang, Jianwei Yang
Summary: This article deals with the low Mach number limit of the compressible Euler-Korteweg equations. It is rigorously justified that solutions of the compressible Euler-Korteweg equations converge to those of the incompressible Euler equations as the Mach number tends to zero. Furthermore, the desired convergence rates are also obtained.
APPLICATIONS OF MATHEMATICS
(2023)
