Article
Chemistry, Analytical
Wenjie Yang, Lili Gong, Ruixin Liu, Ke Wu, Lihua Wang, Guanwei Fan, Haiqiang Jiang, Yunlun Li, Zhixin Tang
Summary: Atherosclerosis is a systemic vascular disease that is difficult to diagnose in its early stages. Researchers have developed a lysosome-targeted fluorescence probe, Lys-HClO, which can assess atherosclerosis and monitor changes in HClO levels. The results suggest that Lys-HClO could be a potential imaging tool for early diagnosis of atherosclerosis.
MICROCHEMICAL JOURNAL
(2024)
Review
Chemistry, Multidisciplinary
Xiaofeng Wu, Rui Wang, Nahyun Kwon, Huimin Ma, Juyoung Yoon
Summary: This article summarizes the methods for in situ imaging of enzymes using activatable fluorescent probes, and proposes that fluorescent probes with both activatable and in situ properties will facilitate high-resolution studies of enzymes in living systems.
CHEMICAL SOCIETY REVIEWS
(2022)
Article
Chemistry, Analytical
Songjiao Li, Ke Yang, Peipei Wang, Ying Liu, Dan Cheng, Longwei He
Summary: Heat stroke is a devastating pathology with high morbidity and mortality rates. Understanding the cytotoxic mechanisms of heat can help prevent or treat stroke-related diseases. The role of lysosomes in heat stroke is poorly understood due to a lack of tools, but recent studies have shown a correlation between lysosome HClO and heat stroke development. To better interpret and treat heat stroke, a reliable real-time tool for tracking HClO levels is necessary, and MB-HClO, a near-infrared probe, shows promise in rapidly detecting lysosome HClO.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Jiankang Gong, Xiaoyu Wang, Hai-En Fan, Jiaxuan Wang, Fan Zhang, Zhiqiang Mao
Summary: This study successfully detected the fluctuation of ONOO- during cellular pyroptosis using the highly sensitive NIR probe Rd700-PN and demonstrated its ability to scout the chemotherapeutic drug's induction of tumor pyroptosis in vivo. It provides an excellent means to investigate the correlation between ONOO- and pyroptosis and screen antitumor drugs activating pyroptosis.
Review
Medicine, General & Internal
Pan Luo, Fu-Qiang Gao, Wei Sun, Jun-You Li, Cheng Wang, Qing-Yu Zhang, Zhi-Zhuo Li, Peng Xu
Summary: Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by synovitis and polyarticular opacity, which can lead to joint damage and irreversible disability. Early diagnosis and effective therapeutic monitoring are crucial for good prognosis of RA. Activatable fluorescent probes are important for detecting and imaging RA biomarkers, such as reactive oxygen/nitrogen species, pH, and cysteine, and there are challenges and prospects for their improvement in RA studies.
MILITARY MEDICAL RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Xiaopeng Fan, Tianbing Ren, Wen Yang, Xiaobing Zhang, Lin Yuan
Summary: A novel dual-modal probe (NO2-CS) was rationally developed and applied for imaging isoniazid-induced liver hypoxia by detecting the over-expressed nitroreductase, which is crucial for the effective monitoring and treatment of drug-induced liver injury.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Leo K. B. Tam, Pui-Chi Lo, Peter Chi Keung Cheung, Dennis K. P. Ng
Summary: A water-soluble 1,2,4,5-tetrazine-substituted carbon-dipyrromethene (C-DIPY) was synthesized and showed fluorescence quenching in phosphate-buffered saline. However, the fluorescence could be restored up to 6.6-fold by adding a bicyclo[6.1.0]non-4-yne (BCN) derivative through an inverse electron-demand Diels-Alder reaction. This bioorthogonal activation was also observed in human glioblastoma cells, enhancing the fluorescence intensity of C-DIPY by 8.7-fold.
CHEMISTRY-AN ASIAN JOURNAL
(2023)
Article
Biophysics
Lijuan Gui, Jun Yan, Junyuan Zhao, Shiya Wang, Yingying Ji, Ji Liu, Jinsheng Wu, Kang Yuan, Heng Liu, Dawei Deng, Zhenwei Yuan
Summary: This study developed a novel ratiometric probe Rx-NCE to detect hypochlorite levels in foam cells and outline the boundaries of atherosclerotic plaques. The innovative application of this probe in distinguishing atherosclerotic blood vessels is also demonstrated. This research has significant value in studying ER stress and atherosclerosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Xiao-Peng Fan, Jing Huang, Tian-Bing Ren, Lin Yuan, Xiao-Bing Zhang
Summary: This study developed a novel near-infrared hemicyanines fluorophore (FJH) with good hydrophilicity and blood circulation, enabling lung imaging. The probe FJH-C showed selectivity and sensitivity towards the ALI indicator (esterase) in vitro and in living cells. This research provides a new method for real-time lung functional imaging and highlights the fluorophore structure with high lung retention.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Lei Yang, Ling Jiang, Feifei Xu, Hongyong Zheng, Miaomiao Liu, Pengfei Shi, Shusheng Zhang, Xiangzhi Song
Summary: Breast cancer has a high incidence rate and mortality rate among women. A second near-infrared (NIR-II) fluorescent probe (BP-A) was developed in this study to detect breast cancer by monitoring the concentration changes of H2S. BP-A showed excellent NIR-II emission and the ability to sensitively and specifically detect H2S in living mice. It also demonstrated the ability to distinguish between healthy mice and those with breast cancer. Therefore, this probe represents a promising and powerful tool for the early clinical detection of breast cancer.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Xi Yang, Yi Ding, Xiaolu Wang, Hongmiao Dai, Haifeng Xu, Bo Wu, Xiaoyang He, Yuxuan Zhang, Xin Li, Yiguang Jin, Chun-Ping Cui, Jiang Peng, Yu Cui, Lingqiang Zhang
Summary: Osteoporosis is a disease characterized by decreased bone mass and strength, with bone mineral density being a critical parameter for diagnosis and monitoring. Through research, a negative correlation between osteoporosis and CKIP-1 was discovered, leading to the development of a new fluorescent probe targeting CKIP-1 for monitoring bone mass changes and therapy responses.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Suya Zhang, Lulu Ning, Zhihui Song, Xinyue Zhao, Feng Guan, Xiao-Feng Yang, Jianjian Zhang
Summary: This study designs an organic nanoprobe capable of real-time imaging of ClO- in early RA. The probe exhibits high selectivity and low detection limit for ClO- levels, and is able to monitor treatment responses and efficacy in a mouse model. The research proposes a universal molecular design strategy for the detection of ClO-, which holds potential for early diagnosis and drug screening for RA.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Zhao Wang, Jiankang Gong, Pengzhan Wang, Jianhua Xiong, Fan Zhang, Zhiqiang Mao
Summary: This study developed a novel activatable AIE fluorescent probe (DPPO-PN) for the detection of ONOO- levels in rheumatoid arthritis (RA). The probe showed excellent sensing abilities for ONOO- and could capture and visualize the fluctuations of intracellular ONOO- levels in real-time using confocal imaging. Moreover, the probe enabled intravital imaging of ONOO- in LPS-induced and CFA-induced RA mouse models.
Article
Chemistry, Applied
Zhe Zhou, Xiaofeng Xia, Zheng Li, Tianshuo Zhou, Erfei Wang, Chao Ma, Cuifen Lu, Junqi Nie, Guichun Yang, Juan Wang, Guorun Fan, Jun Ren, Feiyi Wang
Summary: In this study, a new reductant-resistant fluorescent probe for highly effective tracking of nitroxyl (HNO) in vitro and in vivo in the near-infrared regions was reported. The probe exhibited ultra-sensitivity and high selectivity for HNO and was successfully used to track HNO in living cancer cells and zebrafish models. This work provides a potential tool for in vivo HNO tracking and is helpful for HNO-related pathological studies in living organisms.
Article
Chemistry, Analytical
He-Song Wang, Xiao-Fan Zhang, Han Dong, Qian Chen, Xiao-Qun Cao, Shi-Li Shen
Summary: Ulcerative colitis is a common inflammatory disease related to hypoxia in the colon microenvironment. Researchers have developed a new probe for detecting overexpressed nitroreductase (NTR) and conducting in vivo hypoxia imaging.
ANALYTICA CHIMICA ACTA
(2022)
Article
Urology & Nephrology
Yukari Mae, Tomoaki Takata, Kentaro Yamada, Shintaro Hamada, Marie Yamamoto, Takuji Iyama, Hajime Isomoto
Summary: Sarcopenia is a risk factor for mortality in hemodialysis patients, and current diagnostic modalities have limitations. This study established creatinine generation rate (CGR) as a simple index for assessing muscle mass in hemodialysis patients, which was found to be positively correlated with skeletal muscle mass and predictive of long-term survival.
CLINICAL AND EXPERIMENTAL NEPHROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tomoaki Takata, Shintaro Hamada, Yukari Mae, Takuji Iyama, Ryohei Ogihara, Misako Seno, Kazuomi Nakamura, Miki Takata, Takaaki Sugihara, Hajime Isomoto
Summary: This study found that the production of uromodulin is regulated during water deprivation in vivo, and it activates AQP2 by suppressing endocytosis, enhancing its apical trafficking. This finding provides new insights into the cross-talk between the thick ascending limb and the collecting duct, and suggests that modulation of uromodulin may be a promising approach for diuresis and hypertension treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Takakazu Nagahara, Takaaki Sugihara, Takuya Kihara, Suguru Ikeda, Yoshiki Hoshino, Yukako Matsuki, Takuki Sakaguchi, Hiroki Kurumi, Takumi Onoyama, Tomoaki Takata, Tomomitsu Matono, Naoyuki Yamaguchi, Hajime Isomoto
Summary: This study investigated the characteristics and prognosis of double-negative HCC (DNHC) in higher stages of hepatocellular carcinoma (HCC) using AFP and DCP as tumor markers. It was found that DNHC in higher BCLC stages showed smaller tumor size and had better survival rates.
Article
Biochemistry & Molecular Biology
Shintaro Hamada, Yukari Mae, Tomoaki Takata, Hinako Hanada, Misaki Kubo, Sosuke Taniguchi, Takuji Iyama, Takaaki Sugihara, Hajime Isomoto
Summary: Steatosis is the underlying cause of non-alcoholic steatohepatitis and chronic kidney disease. Endoplasmic reticulum (ER) stress induced by steatosis leads to kidney injury. This study investigated the therapeutic potential of 5-aminolevulinic acid (5-ALA) in alleviating ER stress and lipotoxicity in human renal cells. The results showed that 5-ALA treatment increased the expression of heme oxygenase-1 (HO-1) and reduced the expression of ER stress markers, thereby protecting against lipotoxicity-induced renal injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Rheumatology
Satoshi Omura, Takashi Kida, Hisashi Noma, Atsuhiko Sunaga, Hiroaki Kusuoka, Masatoshi Kadoya, Daiki Nakagomi, Yoshiyuki Abe, Naoho Takizawa, Atsushi Nomura, Yuji Kukida, Naoya Kondo, Yasuhiko Yamano, Takuya Yanagida, Koji Endo, Shintaro Hirata, Kiyoshi Matsui, Tohru Takeuchi, Kunihiro Ichinose, Masaru Kato, Ryo Yanai, Yusuke Matsuo, Yasuhiro Shimojima, Ryo Nishioka, Ryota Okazaki, Tomoaki Takata, Takafumi Ito, Mayuko Moriyama, Ayuko Takatani, Yoshia Miyawaki, Toshiko Ito-Ihara, Nobuyuki Yajima, Takashi Kawaguchi, Wataru Fukuda, Yutaka Kawahito
Summary: This retrospective cohort study found that decreased serum IgG levels caused by remission-induction therapy of AAV were associated with the development of severe infections.
Article
Medicine, General & Internal
Takuya Kihara, Takaaki Sugihara, Suguru Ikeda, Yukako Matsuki, Hiroki Koda, Takumi Onoyama, Tomoaki Takata, Takakazu Nagahara, Hajime Isomoto
Summary: Jaundice is a symptom of excess circulating bilirubin, which can be accurately identified and quantified using transconjunctiva optical imaging. This technology, utilizing an ordinary smartphone and an algorithm, can be a helpful diagnostic tool for telemedicine or self-medication.
Article
Rheumatology
Hideaki Sofue, Takashi Kida, Aiko Hirano, Satoshi Omura, Masatoshi Kadoya, Daiki Nakagomi, Yoshiyuki Abe, Naoho Takizawa, Atsushi Nomura, Yuji Kukida, Naoya Kondo, Yasuhiko Yamano, Takuya Yanagida, Koji Endo, Shintaro Hirata, Kiyoshi Matsui, Tohru Takeuchi, Kunihiro Ichinose, Masaru Kato, Ryo Yanai, Yusuke Matsuo, Yasuhiro Shimojima, Ryo Nishioka, Ryota Okazaki, Tomoaki Takata, Takafumi Ito, Mayuko Moriyama, Ayuko Takatani, Yoshia Miyawaki, Toshiko Ito-Ihara, Nobuyuki Yajima, Takashi Kawaguchi, Kazuki Fujioka, Wataru Fujii, Takahiro Seno, Makoto Wada, Masataka Kohno, Yutaka Kawahito
Summary: Low-dose intravenous cyclophosphamide (7.5-12.5 mg/kg) may result in fewer infections and similar relapse rates compared with the conventional regimen (>12.5 mg/kg).
MODERN RHEUMATOLOGY
(2023)
Review
Virology
Yukako Matsuki, Takaaki Sugihara, Takuya Kihara, Tatsuru Kawakami, Tsuyoshi Kitaura, Tomoaki Takata, Takakazu Nagahara, Kai Fujita, Masayuki Hirai, Masaru Kato, Koichiro Kawaguchi, Hajime Isomoto
Summary: COVID-19 is not limited to respiratory involvement and can lead to multiorgan failure. This case report highlights the development of acute liver failure and rhabdomyolysis in a COVID-19 patient, emphasizing the significance of COVID-19 in the occurrence of multiorgan failure.
Article
Medicine, General & Internal
Naoyuki Yamaguchi, Takuki Sakaguchi, Hajime Isomoto, Tatsuo Inamine, Haruka Ueda, Daisuke Fukuda, Ken Ohnita, Tsutomu Kanda, Hiroki Kurumi, Kayoko Matsushima, Tatsuro Hirayama, Kazuo Yashima, Kazuhiro Tsukamoto
Summary: This study identified SNPs in ATG5, ATG10, ATG12, and ATG16L1 genes associated with gastric mucosal atrophy (GMA). Among these SNPs, rs6431655, rs6431659, and rs4663136 in ATG16L1 and rs26537 in ATG12 were independently associated with GMA.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biology
Tomoaki Takata, Takuji Iyama, Kentaro Yamada, Hajime Isomoto
Summary: Researchers have developed a urinary fluorescence imaging technique to assess the pathology of kidney diseases by analyzing fluorescent images of urine. This technique could be used as a non-invasive qualitative assessment tool for identifying underlying etiologies in patients with kidney diseases, particularly those with diabetes.
Article
Medicine, Research & Experimental
Shotaro Hoi, Masaya Ogawa, Chishio Munemura, Tomoaki Takata, Hajime Isomoto
Summary: We report a case of SARS-CoV-2 vaccine-induced atypical anti-GBM nephritis that developed after the first dose and was unresponsive to immunosuppressive therapy.
YONAGO ACTA MEDICA
(2023)
Article
Biology
Tomoaki Takata, Hajime Isomoto, Takuji Iyama, Kentaro Yamada
Summary: Researchers have developed a fluorescent-based imaging technique for the on-site assessment of renal biopsy specimens, which can distinguish the distribution of glomeruli in the cortex.
Article
Rheumatology
Yasuhiro Shimojima, Dai Kishida, Takanori Ichikawa, Takashi Kida, Nobuyuki Yajima, Satoshi Omura, Daiki Nakagomi, Yoshiyuki Abe, Masatoshi Kadoya, Naoho Takizawa, Atsushi Nomura, Yuji Kukida, Naoya Kondo, Yasuhiko Yamano, Takuya Yanagida, Koji Endo, Shintaro Hirata, Kiyoshi Matsui, Tohru Takeuchi, Kunihiro Ichinose, Masaru Kato, Ryo Yanai, Yusuke Matsuo, Ryo Nishioka, Ryota Okazaki, Tomoaki Takata, Takafumi Ito, Mayuko Moriyama, Ayuko Takatani, Yoshia Miyawaki, Toshiko Ito-Ihara, Takashi Kawaguchi, Yutaka Kawahito, Yoshiki Sekijima
Summary: This study investigated the characteristics of hypertrophic pachymeningitis (HP) in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). GPA was predominantly observed in patients with HP, and patients with HP in newly diagnosed AAV showed significantly higher PR3-ANCA positivity. Ear and eye manifestations may be implicated in HP development.
ARTHRITIS RESEARCH & THERAPY
(2022)
Meeting Abstract
Gastroenterology & Hepatology
Takashi Hasegawa, Takaaki Sugihara, Yoshiki Hoshino, Ryohei Tarumoto, Yukako Matsuki, Tsutomu Kanda, Tomoaki Takata, Takakazu Nagahara, Tomomitsu Matono, Hajime Isomoto
Review
Medicine, Research & Experimental
Tomoaki Takata, Yukari Mae, Takaaki Sugihara, Hajime Isomoto
Summary: This review article provides a comprehensive understanding of the clinical presentation, investigations, histopathology, and treatment/management of infective endocarditis-associated glomerulonephritis, emphasizing the importance of considering this differential diagnosis for patients with fever of unknown origin and acute renal failure symptoms.
YONAGO ACTA MEDICA
(2022)
