Article
Biology
Hidehiko Kawai, Ren Iwata, Shungo Ebi, Ryusei Sugihara, Shogo Masuda, Chiho Fujiwara, Shingo Kimura, Hiroyuki Kamiya
Summary: This study presents an advanced method for mutation analysis using the supF gene. The method is simple, versatile, and cost-effective, and provides valuable insights into mutation frequencies and spectra. The results reveal previously undetected mutations and offer a novel perspective on the mechanisms of mutagenesis.
Article
Biochemical Research Methods
Quanhu Sheng, Hui Yu, Mingrui Duan, Scott Ness, Jiapeng He, Huining Kang, Limin Jiang, John J. Wyrick, Peng Mao, Yan Guo
Summary: UV radiation can lead to skin cancers by causing DNA damage, and the CPD-Seq technique provides a high-throughput method to study the mutagenesis mechanisms associated with UV damage. CPDSeqer protocol has been developed to assist researchers in processing CPD-Seq data efficiently and effectively.
Article
Plant Sciences
Srijan Jhingan, Avneesh Kumar, Hans-Joachim Harloff, Felix Dreyer, Amine Abbadi, Katrin Beckmann, Christian Obermeier, Christian Jung
Summary: Induced mutations are important sources of genetic variation in plant breeding. In this study, a rapeseed M-2 population was obtained by treating the parent cultivar 'Express' with EMS, and whole genomes were sequenced from 497 M-2 families. The mutation frequencies varied across chromosomes, and the majority of detected mutations were canonical transitions characteristic of EMS mutagenesis. The sequenced resource described in this study provides valuable information for functional gene studies in rapeseed breeding.
Article
Agricultural Engineering
Jinesh Patel, Rahul Chandnani, Sameer Khanal, Jeevan Adhikari, Nino Brown, Peng W. Chee, Don C. Jones, Andrew H. Paterson
Summary: This study improved cotton fiber quality by using chemically induced mutants, enhancing traits such as fiber length, strength, and uniformity. The mutants showed potential for fiber quality beyond the levels of current elite varieties.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Plant Sciences
Chong Tan, Jie Ren, Lin Wang, Xueling Ye, Wei Fu, Jiamei Zhang, Meng Qi, Hui Feng, Zhiyong Liu
Summary: This study identified a stable early-bolting mutant, ebm3, in Chinese cabbage, with a mutation in the BraA04g017190.3 C gene encoding histone methyltransferase CLF. These findings improve understanding of the genetic basis of bolting and flowering in Chinese cabbage, and may aid in genetic improvement efforts.
Article
Energy & Fuels
Yimeng Lin, Yunye Zhang, Yang Fan, Chang Li, Hongzhi Ling, Wenxiang Ping, Jingping Ge
Summary: The study investigated the allocation of carbon flow from starch to lipid synthesis in algal cells. A mutant strain showed enhanced lipid content and productivity, as well as a favorable fatty acid composition for biodiesel production.
BIOENERGY RESEARCH
(2023)
Article
Plant Sciences
Wei Yan, Xing Wang Deng, Chengwei Yang, Xiaoyan Tang
Summary: By investigating 52 rice EMS mutants, it was found that EMS exhibits a preference for G/C sites with higher GC contents in flanking sequences. The composition of local dinucleotides and trinucleotides is also associated with the efficiency of EMS mutagenesis.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Horticulture
Yue Gao, Gaoyang Qu, Shengnan Huang, Zhiyong Liu, Meidi Zhang, Wei Fu, Jie Ren, Hui Feng
Summary: This study compared the effects of EMS mutagenesis on generating mutants in Chinese cabbage using two approaches. It was found that seed EMS mutagenesis had a higher mutation frequency and was more suitable for generating a large-scale mutant library, while microspore EMS mutagenesis was conducive to rapidly obtaining homozygous mutants.
Article
Agriculture, Multidisciplinary
Belay Tilahun Tadesse, Ge Zhao, Paul Kempen, Christian Solem
Summary: This study discovered that a mutated strain of Streptococcus thermophilus can efficiently hydrolyze lactose using whole-cell lactase catalysts. Genome sequencing revealed a mutation that affects the growth of the strain and increases its lactose hydrolyzing ability. These findings could potentially lead to the production of sweet lactose-reduced yoghurt without the need for expensive purified lactase enzymes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agronomy
Rustem Ustun, Bulent Uzun
Summary: This study developed a novel herbicide-resistant soybean through the use of EMS mutagen. The mutagen was applied to the soybean seeds, resulting in the selection of a single resistant mutant. The AHAS gene regions of the mutant progeny were analyzed, revealing specific nucleotide changes and amino acid alterations responsible for herbicide resistance.
Article
Agronomy
Wenfeng Weng, Yong Tang, Ruiqi Xiong, Qing Bai, Anjing Gao, Xin Yao, Weijiao Wu, Chao Ma, Jianping Cheng, Jingjun Ruan
Summary: In this study, a mutant library of 'Hongyingzi' liquor-making sorghum variety was created using seeds treated with 0.5% EMS, resulting in 415 M3 generation plants. Nine important phenotypic mutant lines were obtained, including plant type, leaf blade, spike, glume, growth period, fertility, plant height, and drought resistance. Through PCR sequencing, a mutation frequency of 1/99.02 kb was observed in the SbGA2ox3 gene, with one plant experiencing missense mutations of different amino acid types.
Article
Plant Sciences
Karen Thulasi Devendrakumar, Madeleine Goldstein, James Kronstad, Xin Li
Summary: Agrobacterium-mediated transformation is a method that randomly inserts T-DNA into plant genomes, but this insertion may disrupt gene function. We report a phenomenon of reversion of intronic T-DNA mutant phenotypes, highlighting the need for caution when using such mutants and emphasizing the importance of using irreversible null mutants in genetic analysis.
Article
Biochemistry & Molecular Biology
Dongzhi Wang, Yongpeng Li, Haojie Wang, Yongxin Xu, Yiman Yang, Yuxin Zhou, Zhongxu Chen, Yuqing Zhou, Lixuan Gui, Yi Guo, Chunjiang Zhou, Wenqiang Tang, Shuzhi Zheng, Lei Wang, Xiulin Guo, Yingjun Zhang, Fa Cui, Xuelei Lin, Yuling Jiao, Yuehui He, Junming Li, Fei He, Xigang Liu, Jun Xiao
Summary: In this study, a library for the popular wheat variety KN9204 was created using EMS mutagenesis, providing a comprehensive understanding of its genome, transcriptome, and epigenome. Exome capture sequencing revealed a high mutation rate in coding genes, with an average of 1383 EMS-type SNPs per line. New allelic variations for important agronomic trait-related genes were identified, and altered sensitivity to drought and salinity stress was observed in certain NAC transcription factors. This research provides valuable resources for functional genomics research and genetic manipulation of wheat.
PLANT COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Yi Lin, Jiawen Lu, Zilu Yang, Tianming Wang, Hongbiao Li, Shenfei Sha, Zhenmin Liu, Yueliang Zhao, Liping Wang
Summary: In this study, the antimicrobial activity of P. pentosaceus C-2-1 was enhanced by UV mutagenesis, resulting in the production of a mutant strain, C23221, with significantly higher antimicrobial activity. A comparison of the genomes of C23221 and the wild-type strain revealed unique genes and proteins related to bacteriocin biosynthesis in the mutant strain. This study provides a genetic basis for future genetic engineering strategies to increase antimicrobial production in P. pentosaceus C-2-1.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Public, Environmental & Occupational Health
Liqiu Ma, Fuquan Kong, Kai Sun, Ting Wang, Tao Guo
Summary: Radiation mutation breeding, which has been used for nearly 100 years, successfully improves crops by increasing genetic variation. Despite advancements in gene editing, mutation-induced germplasm diversity remains essential in modern and classical radiation breeding to produce random mutations in the whole genome and address challenges in global food production.
FRONTIERS IN PUBLIC HEALTH
(2021)
Article
Environmental Sciences
Melvin S. Samuel, Kirankumar Venkat Savunthari, Selvarajan Ethiraj
Summary: The Cu(II) metal-organic frameworks based on 1,3,5-benzenetricarboxylic acid were successfully synthesized by the hydrothermal method, exhibiting pyramid-shaped morphology and high specific surface area. The photocatalytic activity of Cu-3(BTC)(2) on Rhodamine B degradation under visible light irradiation was significantly enhanced, showing great potential for environmental applications in water pollution treatment. Additionally, its recyclability was confirmed through multiple cycles of testing.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Review
Engineering, Environmental
Melvin S. Samuel, E. Selvarajan, Ankur Sarswat, Harshiny Muthukumar, Jaya Mary Jacob, Malavika Mukesh, Arivalagan Pugazhendhi
Summary: The demand for freshwater is expected to rise in the next few decades due to global population growth and industrial development. Arsenic contamination in groundwater is a major worldwide issue, with various methods such as adsorption and filtration being used to address it. Nanomaterials and biopolymers are widely used for arsenic removal, with studies focusing on their sorption mechanisms and performance.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Melvin S. Samuel, Kirankumar Venkatesan Savunthari, Narendhar Chandrasekar, Ramachandran Balaji, Ethiraj Selvarajan
Summary: This study investigates the adsorption characteristics of pharmaceutical pollutants using CuBTC@NH2 composite. The post-synthetic modification of CuBTC@NH2 greatly improves the physicochemical characteristics of the MOF, resulting in differences in the adsorption behavior of pharmaceutical pollutants such as IBF and ACE from aqueous solutions. The results demonstrate the potential of the CuMOF@NH2 composite to remove pharmaceutical pollutants from toxic wastewater.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2022)
Article
Environmental Sciences
Alexander Pinky Steffi, Ramachandran Balaji, Natarajan Prakash, Thangavel Pichaiappa Rajesh, Selvarajan Ethiraj, Melvin S. Samuel, Ashok Kumar Nadda, Narendhar Chandrasekar
Summary: Carbon-based nano-structured materials are widely used in composite coating for anti-corrosive reinforcement. The highly efficient gC(3)N(4)/SiO2/TiO2 ternary nanocomposite enhances the physical, chemical and mechanical properties of the coating, making it suitable for real-world applications.
Article
Environmental Sciences
Melvin S. Samuel, K. Mohanraj, Narendhar Chandrasekar, Ramachandran Balaji, Ethiraj Selvarajan
Summary: This study evaluated the photocatalytic performance of the GO/Cu-3(BTC)(2)/Fe3O4 photocatalyst for the degradation of AFB1. The results showed that the photocatalyst had a high degradation efficiency of AFB1 due to its large surface area and improved performance. It also demonstrated high capturing capacity and photoactivity synergy.
Article
Materials Science, Multidisciplinary
Alexander Pinky Steffi, Ramachandran Balaji, Narendhar Chandrasekar, Natarajan Prakash, Thangavel Pichaiappa Rajesh, Selvarajan Ethiraj, Melvin S. Samuel, Srikanth Vuppala
Summary: Researchers have successfully synthesized an anticorrosive ternary nanocomposite coating that protects metal surfaces, prevents corrosion damage, and exhibits high corrosion resistance.
DIAMOND AND RELATED MATERIALS
(2022)
Article
Environmental Sciences
Ramachandran Balaji, Selvarasu Maheshwaran, Shen-Ming Chen, Elayappan Tamilalagan, Narendhar Chandrasekar, Selvarajan Ethiraj, Melvin S. Samuel
Summary: A dual mode catalytic strip equipped with 2D BiOI nanostructures was constructed for the dual mode sensing of hazardous trichlomphenol (TCP). The BiOI nanostructures exhibited superior catalytic properties and sensitivity in electrochemical and SERS modes for the detection of TCP.
ENVIRONMENTAL POLLUTION
(2022)
Article
Environmental Sciences
Selvarasu Maheshwaran, Ramachandran Balaji, Shen-Ming Chen, Yo-Shiuan Chang, Elayappan Tamilalagan, Narendhar Chandrasekar, Selvarajan Ethiraj, Melvin S. Samuel, Mohanraj Kumar
Summary: In this study, a novel electrocatalyst for electrochemical sensing of Furazolidone (FZE) drug was developed using hydrothermally synthesized BiVO4@MoS2 hierarchical nano-heterojunction composite. The unique composite exhibited excellent electrocatalytic activity in sensing FZE with superior linear detection ranges and a limit of detection far superior to other reported sensors. The BiVO4@MoS2 based sensor is recommended for analyzing real-time samples like human urine and blood serum with appreciable recovery.
ENVIRONMENTAL RESEARCH
(2022)
Article
Environmental Sciences
Natarajan Prakash, Ramachandran Balaji, Saravanan Govindaraju, Alexander Pinky Steffi, Nagendran Santhanalakshmi, Kumar Mohanraj, Ethiraj Selvarajan, Narendhar Chandrasekar, Melvin S. Samuel
Summary: The study successfully synthesized S15/m-Co3O4 nanoarcs, confirming their excellent electrochemical performance and photocatalytic degradation capabilities. This material exhibited a specific capacity of 87.5 C/g in a 1 M KOH solution, with a retention capacity of 87% after 1000 cycles, and can also serve as a catalyst for the degradation of dye molecules through photocatalysis.
ENVIRONMENTAL RESEARCH
(2022)
Review
Chemistry, Physical
Melvin S. Samuel, Madhumita Ravikumar, Ashwini John J., Ethiraj Selvarajan, Himanshu Patel, P. Sharath Chander, J. Soundarya, Srikanth Vuppala, Ramachandran Balaji, Narendhar Chandrasekar
Summary: The green synthesis of metal oxide nanoparticles is an important research area that can enhance their biocompatibility and applications in the medical and environmental sectors. Utilizing microorganisms and plant extracts for bio-synthesis can reduce toxicity and bring new insights into nanoparticle production. Current research primarily focuses on the application of nanoparticles in cancer therapy and explores their potential for clinical use.
Article
Environmental Sciences
Selvarasu Maheshwaran, Vengudusamy Renganathan, Shen-Ming Chen, Ramachandran Balaji, C. R. Kao, Narendhar Chandrasekar, Selvarajan Ethiraj, Melvin S. Samuel, Muthusamy Govarthanan
Summary: A hybrid AgWO4-rGO nanocomposite electrode was successfully constructed by hydrothermal method for the rapid electrochemical detection of harmful pesticides. The nanocomposite exhibited excellent catalytic activity and ultrasensitive detection limit, with good selectivity and reproducibility for real water samples.
Review
Biochemistry & Molecular Biology
Susan Aishwarya Suresh, Selvarajan Ethiraj, K. N. Rajnish
Summary: L-Asparaginases are widely used in the treatment of Acute Lymphoblastic Leukemia (ALL) and reducing acrylamide in the food industry. However, the enzyme's side effects and the risk of acrylamide-induced carcinogenesis limit its application. Enzyme immobilization and gene editing techniques are important approaches to improve its efficacy.
MOLECULAR BIOLOGY REPORTS
(2022)
Review
Environmental Sciences
J. Ashwini John, Melvin S. Samuel, Muthusamy Govarthanan, Ethiraj Selvarajan
Summary: Marine actinobacteria can produce cellulases with high stability, which can convert cellulose into glucose for biofuel production. This review provides a comprehensive overview of various applications of cellulases from marine actinobacteria, including screening for novel cellulase genes, enhancing production, and immobilizing the enzyme for industrial usage.
ENVIRONMENTAL RESEARCH
(2022)
Review
Environmental Sciences
Rashmi Singh, Melvin S. Samuel, Madhumita Ravikumar, Selvarajan Ethiraj, Venkatesan Savunthari Kirankumar, Mohanraj Kumar, R. Arulvel, Sagadevan Suresh
Summary: This article provides an overview of abundant carbon-based nanomaterials in both the inorganic and organic worlds, including benzene, diamond, graphite, fullerene, and carbon nanotubes. The synthesis, characteristics, and various applications of graphene are explored, with a focus on ecologically friendly production techniques. Recent advancements in the detection and treatment of organic contaminants and heavy metals using nanomaterials are also discussed. The article presents the current state of the field and is considered helpful for anyone interested in nanomaterials and related materials.
Article
Engineering, Environmental
Selvarasu Maheshwaran, Ramachandran Balaji, Shen-Ming Chen, Ying-Chih Liao, Narendhar Chandrasekar, Selvarajan Ethiraj, Melvin S. Samuel
Summary: In this study, sonochemically combined 2D-0D Ti3AlC2@SmVO4 nanocomposites were successfully used for the electrochemical detection of Sulfathiazole. Thorough characterization of the TASV heterojunction composites revealed their excellent electrochemical properties and remarkable detection range for SFZ sensing. The performance of TASV sensors in real-world conditions was also highly admirable.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
