Article
Biotechnology & Applied Microbiology
Barbara Kubickova, Joerg A. Schenk, Franziska Ramm, Kornelija Markuskiene, Jochen Reetz, Paul Dremsek, Paulius Lukas Tamosiunas, Laima Cepulyte, Hoai Anh Trinh, Johannes Scholz, Henry Memczak, Marc Hovestaedt, Rene Ryll, Rasa Petraityte-Burneikiene, Victor M. Corman, Anika Andersson, Dietmar Becher, Martin H. Groschup, Stefan Kubick, Frank Sellrie, Reimar Johne, Rainer G. Ulrich
Summary: A hepatitis E virus (HEV) genotype 3-specific monoclonal antibody (mAb) was successfully generated by immunising BALB/c mice with a carboxy-terminal part of its capsid protein expressed in Escherichia coli. The mAb, named G117-AA4, showed strong reactivity with homologous and heterologous HEV-3 capsid proteins, as well as with capsid protein derivatives from other genotypes and related viruses. This novel mAb may prove valuable for further studies on HEV infections and diagnostic purposes.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Mengnan Fan, Yuhang Luo, Beibei Zhang, Jiaxi Wang, Tianxiang Chen, Baoyuan Liu, Yani Sun, Yuchen Nan, Julian A. Hiscox, Qin Zhao, En-Min Zhou
Summary: The study confirmed the direct interaction between HEV and CDC42, and identified different CDC42 downstream signaling pathways used by non-enveloped and quasi-enveloped HEV during infection. These results provide new insights into the life cycle of HEV in host cells and offer a new drug design target for combating HEV infection.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Takashi Nishiyama, Koji Umezawa, Kentaro Yamada, Masaharu Takahashi, Satoshi Kunita, Mulyanto, Isao Kii, Hiroaki Okamoto
Summary: The study compared the molecular size of the ORF2 protein among fecal HEV, cell-culture-generated HEV, and treated HEVcc, revealing that the ORF2 proteins associated with fecal HEV were C-terminally truncated and showed the same size as treated HEVcc. This sheds light on the maturation of nonenveloped HEV and is important for understanding the HEV lifecycle.
Article
Virology
Giulia Pezzoni, Lidia Stercoli, Eleonora Pegoiani, Emiliana Brocchi
Summary: In the study evaluating the antigenic properties of HEV ORF2 proteins, 37 MAbs were raised, with one antigenic region competing with positive sera and efficiently recognizing the recombinant antigen. The ORF2 portion from 394-608 aa contains most immunogenic epitopes, with 85% of positive sera reacting to the region from 461-544 aa, while only 5% of selected sera reacted to the ORF3 antigen.
Review
Microbiology
Zhaobin Zhou, Yinqian Xie, Chunyan Wu, Yuchen Nan
Summary: Hepatitis E virus (HEV) is a zoonotic pathogen responsible for acute hepatitis outbreaks globally. The HEV-ORF2 protein, in addition to forming the viral capsid, plays multiple roles in viral replication and pathogenesis, making it a potential target for vaccine development and treatment of HEV infection.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Tejaswini Mahesh Deshmukh, Manisha Tukaram Dudhmal, Neeta Changdeo Thorat, Prakash Dnyaneshwar Sarje, Atul M. Walimbe, Kavita Satish Lole
Summary: This study evaluated the performance of different IgM detection enzyme-linked immunosorbent assays (ELISAs) for hepatitis E virus (HEV) infection. The results showed that both ORF2 and T1NE ELISAs were equally efficient in diagnosing HEV infections. T1NEp proved to be an excellent tool in HE sero-diagnosis and is worth exploring in the development of simple rapid tests.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Virology
Simon D. Lytton, Rakibul Hassan Bulbul, Kishor Barua, Mosa C. Begum, Bani Chowdhury, Zahirul Islam, Kazi Ishrak Faiyaz, Shahnawaz Khan Chandan, Ahmed Shakeel, Olfert Landt, Rashid Mamunur
Summary: In an HEV outbreak in Chittagong, Bangladesh, the HEV-Ag ELISA proved to be a reliable and practical diagnostic tool for acute HEV infections, showing higher positivity in viral RNA-positive cases and a positive correlation with viremia.
JOURNAL OF CLINICAL VIROLOGY
(2021)
Article
Virology
Huimin Bai, Michiyo Kataoka, Yasushi Ami, Yuriko Suzaki, Naokazu Takeda, Masamichi Muramatsu, Tian-Cheng Li
Summary: Rabbit hepatitis E virus (HEV) is a novel HEV which belongs to genotype 3 in the Orthohepevirus A species. Through expressing truncated rabbit HEV ORF2 proteins, two types of virus-like particles were obtained, with RsVLPs showing stronger antigenic cross-reactivity against different HEV genotypes. Immunization with RsVLPs completely protected rabbits from challenge by rabbit HEV, suggesting potential for vaccine development.
Article
Cell Biology
Daniela Lenggenhager, Jonas Grossmann, Jerome Gouttenoire, Christine Sempoux, Achim Weber
Summary: Immunohistochemistry for HEV ORF2 protein is a useful tool for tissue-based diagnosis of hepatitis E. However, we report an unexpected immunoreactivity of the HEV ORF2 antibody to CMV proteins. The staining pattern of CMV-infected cells is limited to the nucleus, in contrast to the cytoplasmic and occasional canalicular positivity observed in hepatitis E.
Article
Biology
Gergana Zahmanova, Milena Mazalovska, Katerina Takova, Valentina Toneva, Ivan Minkov, Hadrien Peyret, George Lomonossoff
Summary: This study demonstrates that HBcAg is a promising carrier platform for neutralizing epitopes of hepatitis E virus (HEV), and the chimeric HBcAg/HEV VLPs could be a candidate for a bivalent vaccine.
Article
Clinical Neurology
Miriam Fritz-Weltin, Lisa Niedermeier, Estelle Frommherz, Nora Isenmann, Benedikt Csernalabics, Tobias Boettler, Christoph Neumann-Haefelin, Dominique Endres, Marcus Panning, Benjamin Berger
Summary: This retrospective study systematically screened 104 patients with Bell's palsy for acute HEV infection, but found no association between acute HEV infection and Bell's palsy. However, a high prevalence of anti-HEV IgG in Bell's palsy patients was observed, which increased with age.
EUROPEAN JOURNAL OF NEUROLOGY
(2022)
Article
Microbiology
Kevin Hervouet, Martin Ferrie, Maliki Ankavay, Claire Montpellier, Charline Camuzet, Virginie Alexandre, Aicha Dembele, Cecile Lecoeur, Arnold Thomas Foe, Peggy Bouquet, David Hot, Thibaut Vausselin, Jean-Michel Saliou, Sophie Salome-Desnoulez, Alexandre Vandeputte, Laurent Marsollier, Priscille Brodin, Marlene Dreux, Yves Rouille, Jean Dubuisson, Cecile-Marie Aliouat-Denis, Laurence Cocquerel
Summary: Hepatitis E virus (HEV) is a common cause of acute viral hepatitis worldwide and can become chronic in immunocompromised individuals. The ORF2 protein encoded by the HEV genome plays a crucial role in the virus lifecycle and interactions with the host. This study identified an Arginine-Rich Motif (ARM) in the N-terminal region of ORF2 that controls the fate and functions of different isoforms of ORF2. The ARM regulates nuclear translocation, signal peptide topology, glycosylation, and host cell membrane interactions of ORF2.
Article
Multidisciplinary Sciences
Bacary Djilocalisse Sadio, Martin Faye, Marco Kaiser, Maryam Diarra, Fanny Balique, Cheikh Tidiane Diagne, Oumar Faye, Moussa Moise Diagne, Gamou Fall, Oumar Ndiaye, Cheikh Loucoubar, Abdourahmane Sow, Ousmane Faye, Adama Faye, Cheikh Saad Bouh Boye, Amadou Alpha Sall
Summary: The rapid proliferation of traditional gold mining sites in the Kedougou region has led to a Hepatitis E virus outbreak. The study found a high rate of HEV infection among suspected cases, with a case fatality rate in pregnant women. Further research is needed to identify the virus reservoir and environmental risk factors.
SCIENTIFIC REPORTS
(2022)
Article
Biology
Yakubu Egigogo Raji, Ooi Peck Toung, Niazlin Mohd Taib, Zamberi Bin Sekawi
Summary: HEV is a global public health and economic challenge affecting both humans and animals worldwide. Recent studies suggest endemicity of HEV in both developing and industrialized nations. The virus has diverse transmission routes and potential risk factors.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Immunology
Andrew S. Azman, Kishor Kumar Paul, Taufiqur Rahman Bhuiyan, Aybuke Koyuncu, Henrik Salje, Firdausi Qadri, Emily S. Gurley
Summary: Hepatitis E infections are common throughout Bangladesh, with a national seroprevalence of 20%. Seropositivity increases with age and male sex, and urban areas, including Dhaka, have significantly higher seroprevalence compared to other regions. Strengthening surveillance for hepatitis E, especially in urban areas, can help target interventions effectively.
JOURNAL OF INFECTIOUS DISEASES
(2021)
