Review
Genetics & Heredity
Luoyu Mo, Jun Ma, Yan Xiong, Xianrong Xiong, Daoliang Lan, Jian Li, Shi Yin
Summary: This review summarizes the factors affecting the in vitro maturation and developmental ability of yak oocytes in recent years, providing a theoretical basis for in vitro fertilization and embryo production in yaks.
Review
Biochemistry & Molecular Biology
Pawel Kordowitzki, Gabriela Sokolowska, Marta Wasielak-Politowska, Agnieszka Skowronska, Mariusz T. Skowronski
Summary: The oocyte plays a crucial role in embryo development, with cell-to-cell and cell-to-matrix communications being key for oocyte's developmental competence. Pannexins and Connexins are families of channels that are important for various physiological and pathological processes related to oocyte development and fertility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Dongmei Deng, Juan Xie, Yin Tian, Ling Zhu, Xuemei Liu, Junxia Liu, Guoning Huang, Jingyu Li
Summary: Acquisition of germinal vesicle (GV) stage oocytes for fertility preservation (FP) offers several benefits over in vivo matured oocyte cryopreservation following ovarian stimulation. Two approaches for GV oocytes are available: vitrification before in vitro maturation (IVM) at the GV stage or post-IVM at the metaphase II (MII) stage. This study found that oocyte vitrification at the MII stage after IVM is more suitable for patients who require immediate FP.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Elisa Serra, Sergio Domenico Gadau, Giovanni Giuseppe Leoni, Salvatore Naitana, Sara Succu
Summary: The study found that oocytes collected in spring from prepubertal ewes have higher developmental competence and vitality in in vitro embryo production, with lower production of reactive oxygen species. The research indicates that the season of collection can affect the developmental competence, energetic status, and tubulin assessment of oocytes, emphasizing the importance of considering the season when collecting prepubertal ovine oocytes for in vitro embryo reproduction programs.
Article
Reproductive Biology
Ashitha Suresh, M. K. Shukla, Dharmendra Kumar, O. P. Shrivastava, Neeraj Verma
Summary: The study shows that the simulated physiological oocyte maturation system significantly enhances the developmental competence of goat oocytes and reduces the intensity of reactive oxygen species and embryonic apoptosis.
Article
Reproductive Biology
Amirhossein Abazarikia, Mahdi Zhandi, Armin Towhidi, Malak Shakeri, Ali Reza Youse, Amir Aliyan
Summary: The study showed that introducing conjugated linoleic acid (CLA) can improve oocyte developmental competence, reduce aberrant spindle organization of heat-stressed oocytes, and does not cause epigenetic alteration in the obtained blastocysts.
Article
Biology
Ignacio Bejarano, Monica Dorado-Silva, Helia Sarmiento-Soto, Nuria Alvarez-Sanchez, Patricia Judith Lardone, Juan Miguel Guerrero, Pascual Sanchez-Martin, Antonio Carrillo-Vico
Summary: Despite advances in assisted reproduction technology, success rates remain low. The study found that the expression of GPX3 in cumulus cells was significantly reduced in embryos that successfully implanted, suggesting its potential as a poor prognostic indicator of implantation success.
Article
Veterinary Sciences
Tian Zhao, Yangyang Pan, Qin Li, Tianyi Ding, Robert Niayale, Tongxiang Zhang, Jinglei Wang, Yaying Wang, Ling Zhao, Xiaohong Han, Abdul Rasheed Baloch, Yan Cui, Sijiu Yu
Summary: This study evaluated the influence of leukemia inhibitory factor (LIF) on the maturation and development ability of yak oocytes and the quality of subsequent blastocysts under in vitro culture settings. The findings revealed that adding 50 ng/mL of LIF during in vitro maturation significantly improved the maturation rate, levels of mitochondria and actin, mRNA transcription levels of anti-apoptotic and antioxidant-related genes, and reduced the generation of reactive oxygen species (ROS) and apoptosis levels in yak oocytes. Blastocysts formed from LIF-treated oocytes showed significantly larger total cell numbers and lower apoptosis rates. Overall, the addition of LIF during in vitro maturation improved the quality and competence of oocytes and subsequent blastocysts in yaks.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Developmental Biology
Zvi Roth
Summary: High temperatures can affect follicles and oocytes, leading to impaired follicle development and compromised competence of oocytes to develop into embryos. Studies have shown various mechanisms of oocyte damage due to heat stress, with only subpopulations of follicles affected rather than the entire ovarian reserve.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2021)
Article
Cell Biology
Ju Hee Lee, Jae Kyun Park, Sook Young Yoon, Eun A. Park, Jin Hyun Jun, Hyunjung J. Lim, Jayeon Kim, Haengseok Song
Summary: Advanced maternal age (AMA) is associated with decreased quality and quantity of oocytes, making older oocytes more vulnerable to cryo-damage during vitrification. The cellular and molecular features of aged vitrified oocytes exhibit aberrations, leading to delayed embryo development. Collectively, current vitrification protocols optimized for young females may need to be modified for aged women.
Review
Obstetrics & Gynecology
Dulama Richani, Kylie R. Dunning, Jeremy G. Thompson, Robert B. Gilchrist
Summary: Metabolism is crucial for oocyte quality, with glucose being essential for COC maturation and energy production. Mitochondria are the main ATP source in oocytes, while cumulus cells play a vital role in maintaining ATP levels. New insights in oocyte lipid metabolism and innovative strategies in IVM have shown positive effects on oocyte competence.
HUMAN REPRODUCTION UPDATE
(2021)
Article
Obstetrics & Gynecology
Alvaro Martinez-Moro, Leopoldo Gonzalez-Brusi, Ismael Lamas-Toranzo, Pilar Gonzalez-Dosal, Felix Rodriguez-Juarez, Pablo Bermejo-Alvarez
Summary: RNA sequencing analysis showed that the transcriptome of cumulus cells is largely unrelated to the embryo's developmental competence, except for a subset of genes that may indicate the ability to develop to the blastocyst stage.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Biochemistry & Molecular Biology
Radek Prochazka, Alexandra Bartkova, Lucie Nemcova, Matej Murin, Ahmed Gad, Katerina Marcollova, Veronika Kinterova, Andrea Lucas-Hahn, Jozef Laurincik
Summary: Culturing porcine oocytes in a medium containing FGF2, LIF and IGF1 (FLI) significantly enhances their developmental potential. Analyzing the time course of MAPK3/1 and AKT activation, as well as gene expression controlled by these kinases, suggests that both MAPK3/1 and AKT play crucial roles in improving oocyte quality in FLI medium.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Developmental Biology
Kelsey Nicole Lockhart, Jessica N. Drum, Ahmed Z. Balboula, Christine M. Spinka, Thomas E. Spencer, M. Sofia Ortega
Summary: This study evaluated the impact of sire on preimplantation embryonic development in cattle and found that embryos derived from low performing sires had an increased proportion of arrest at the five- to six-cell stage and higher rates of cellular stress.
Article
Agriculture, Dairy & Animal Science
Pradeep Krishna Javvaji, Arindam Dhali, Joseph Rabinson Francis, Atul P. Kolte, Sudhir C. Roy, Sellappan Selvaraju, Anjumoni Mech, Veerasamy Sejian
Summary: The study showed that IGF-1 treatment significantly improved the developmental competence of ovine oocytes, enhancing the maturation rate and embryo development. This effect was achieved through the regulation of the PI3K/Akt and apoptosis signaling pathways.
ANIMAL BIOTECHNOLOGY
(2021)
