期刊
VIROLOGY
卷 546, 期 -, 页码 88-97出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2020.04.009
关键词
ZIKV; Lycorine; Alkaloid; Antiviral; AG6 mice; RdRp inhibitor
类别
资金
- Natural Science Foundation of Guangdong Province [2017A030313626]
- Excellent Young Scholars Project of the First Affiliated Hospital of Guangzhou University of Chinese Medicine [2019QN22, 2017ZWB10]
- Science & Technology Planning Project of Guangzhou [201804010029]
- National Nature Science Foundation of China [81973549, 81803813, 81730061, 81471942]
- State Key Laboratory of Natural Medicines [SKLNMKF201906]
- Science & Technology Planning Project of Guangdong Province Office of Education [2018KCXTD007, 2014GKXM032]
- Program of Guangdong Natural Science Foundation of China [S201230006598]
- Key Research Projects of GZUCM First-class Universities and Top Disciplines [A1-AFD018191A17]
- GZUCM First-class Universities and Top Disciplines Scientific Research Team Projects [2019KYTD102]
- Guangdong Province Pearl River Talent Plan Innovation and Entrepreneurship Team Project [2017ZT07Y580]
The emergence and re-emergence of Zika virus (ZIKV), is a cause for international concern. These highly pathogenic arboviruses represent a serious health burden in tropical and subtropical areas worldwide. Despite these burdens, antiviral therapies do not exist, and inhibitors of ZIKV are therefore urgently needed. To elucidate the anti-ZIKV effect of lycorine, we used reverse transcription-quantitative real-time PCR (qRT-PCR), immunofluorescence, (westernwestern) blot, and plaque forming assay to analyse viral RNA (vRNA), viral protein, progeny virus counts, and validated inhibitors in vitro using a variety of cell lines. Additionally, we found that lycorine acts post-infection according to time-of-addition assay, and inhibits RdRp activity. Lycorine protected AG6 mice against ZIKV-induced lethally by decreasing the viral load in the blood. Due to its potency and ability to target ZIKV infection in vivo and in vitro, lycorine might offer promising therapeutic possibilities for combatting ZIKV infections in the future.
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