Article
Radiology, Nuclear Medicine & Medical Imaging
Julien Songeon, Sebastien Courvoisier, Lijing Xin, Thomas Agius, Oscar Dabrowski, Alban Longchamp, Francois Lazeyras, Antoine Klauser
Summary: This study introduces a new artificial intelligence framework, 31P-SPAWNN, for analyzing phosphorus-31 (P-31) magnetic resonance spectra. The experiment demonstrates the reliability and accuracy of 31P-SPAWNN in estimating metabolite concentrations and spectral parameters. It also shows that 31P-SPAWNN has improved quantification and processing time compared to traditional methods.
MAGNETIC RESONANCE IN MEDICINE
(2023)
Article
Engineering, Biomedical
Rianne Schutter, Otis C. van Varsseveld, Veerle A. Lantinga, Merel B. F. Pool, Tim H. Hamelink, Jan Hendrik Potze, Henri G. D. Leuvenink, Christoffer Laustsen, Ronald J. H. Borra, Cyril Moers
Summary: This study introduces a novel technique of using MRI during ex vivo normothermic machine perfusion for renal grafts, allowing detailed real-time visualization and assessment of structural abnormalities, regional flow distribution, renal metabolism, local oxygen availability, and distribution of ex vivo administered cellular therapy. This platform provides an advanced tool for pre-transplant organ assessment and renal physiology and metabolism research.
Review
Medicine, General & Internal
Ruta Zulpaite, Povilas Miknevicius, Bettina Leber, Kestutis Strupas, Philipp Stiegler, Peter Schemmer
Summary: Kidney transplantation is the gold standard treatment for patients with end-stage kidney disease, but the quality of donor organs poses limitations. Ex-vivo machine perfusion technology has great potential in maintaining kidney viability and optimizing organ treatment, serving as an important component of novel techniques in transplant surgery.
FRONTIERS IN MEDICINE
(2021)
Article
Multidisciplinary Sciences
Qing OuYang, Guohai Liang, Xiaoyu Tan, Xiran He, Lin Zhang, Weijian Kuang, Jianxiong Chen, Shaoping Wang, Mingju Liang, Feng Huo
Summary: This study used a nuclear magnetic resonance relaxation analyzer-based assay and ex situ normothermic machine perfusion to assess the viability of porcine DCD donor livers, confirming the superiority of dual hypothermic oxygenated machine perfusion in cold ischemia protection.
SCIENTIFIC REPORTS
(2021)
Review
Biophysics
Douglas L. Rothman, Kevin L. Behar, Ognen A. C. Petroff, Robert G. Shulman
Summary: This paper provides a summary of the early use of ex vivo nuclear magnetic resonance (NMR) in tissue and tissue extract studies conducted by Dr. Robert G. Shulman from 1975 to 1995 at Bell Laboratories and later at Yale University. Ex vivo NMR played an essential role in resonance assignments, concentration quantification, and technological advancements in magnet technology. The paper presents a general strategy developed in the lab for integrating ex vivo NMR in vivo studies, along with illustrative examples.
NMR IN BIOMEDICINE
(2023)
Article
Engineering, Biomedical
Dilyana B. Mangarova, Gergely Bertalan, Jakob Jordan, Julia Brangsch, Avan Kader, Jana Moeckel, Lisa C. Adams, Ingolf Sack, Matthias Taupitz, Bernd Hamm, Juergen Braun, Marcus R. Makowski
Summary: This study demonstrates the use of microscopic multifrequency magnetic resonance elastography to measure the stiffness of abdominal aortic aneurysms with a resolution of 40 microns. The results show that changes in stiffness are closely related to extracellular matrix remodeling. This technique has the potential to provide detailed characterization of AAAs and quantify pathological remodeling of the extracellular matrix.
ACTA BIOMATERIALIA
(2022)
Article
Surgery
Rianne Schutter, Veerle A. Lantinga, Tim L. Hamelink, Merel B. F. Pool, Otis C. van Varsseveld, Jan Hendrik Potze, Jan-Luuk Hillebrands, Marius C. van den Heuvel, Rudi A. J. O. Dierckx, Henri G. D. Leuvenink, Cyril Moers, Ronald J. H. Borra
Summary: The study aimed to characterize the regional distribution of perfusate flow during ex vivo normothermic machine perfusion (NMP) using magnetic resonance imaging (MRI), in order to understand how kidney assessment protocols should be designed. Results showed that at the start of NMP, all kidneys were mainly centrally perfused, and it took time for the outer cortex to reach physiological dominant perfusion state. Early functional NMP quality assessment markers may not reflect actual physiology accurately and should be interpreted with caution.
TRANSPLANT INTERNATIONAL
(2021)
Article
Biochemical Research Methods
Loraine Carolina Goenaga-Mafud, Yordania Matos Gamez, Carolina P. Campos, Jose Dirceu Vollet-Filho, Natalia Mayumi Inada, Cristina Kurachi, Vanderlei Salvador Bagnato
Summary: This article demonstrates the feasibility of reducing bacterial load in a kidney model by using ultraviolet-C as a germicidal agent. The results show that the bacterial load in the perfusate can be fully eliminated in less than 30 minutes of liquid circulation and irradiation. A modeling approach is used to verify the decrease in bacterial load when an organ is present in the circuit.
JOURNAL OF BIOPHOTONICS
(2022)
Article
Biotechnology & Applied Microbiology
Johannes Castelein, Carolina Pamplona, Roberto Armstrong Junior, Marina Vidal dos Santos, Ingolf Sack, Rudi Dierckx, Cyril Moers, Ronald Borra
Summary: This study investigates the effect of tissue perfusion on renal stiffness and tissue fluidity using multifrequency magnetic resonance elastography. The results show a strong correlation between perfusion parameters (blood pressure and flow) and stiffness and fluidity in the renal cortex. In the medulla, significant correlations were observed between perfusion parameters and stiffness. These findings highlight the perfusion dependency of renal stiffness and fluidity, demonstrating the sensitivity of tomoelastography to fluid pressure.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Enlin Qian, Pavan Poojar, Maggie Fung, Zhezhen Jin, John Thomas Vaughan Jr, Devashish Shrivastava, David Gultekin, Tiago Fernandes, Sairam Geethanath
Summary: The study aims to demonstrate the first application of T-1-based magnetic resonance thermometry using magnetic resonance fingerprinting (MRFT). The temperature estimation of MRFT is compared with proton resonance frequency shift (PRFS) thermometry on ex vivo bovine muscle. The feasibility of MRFT in predicting temperature on ex vivo bovine muscles with deep brain stimulation (DBS) lead is shown.
PHYSICS IN MEDICINE AND BIOLOGY
(2023)
Article
Physiology
Darya Morozov, Neda Parvin, Jennifer R. Charlton, Kevin M. Bennett
Summary: This study demonstrated the sensitivity of diffusion magnetic resonance imaging (dMRI) to measure tubular pathologies in the kidney, offering a noninvasive approach to assess kidney health. By utilizing Monte Carlo simulations, biophysical modeling, and application in a mouse model of acute kidney injury, dMRI was shown to accurately measure tubular diameters and detect pathological changes in the kidney cortex. The findings suggest that dMRI may serve as a new noninvasive biomarker for tubular pathology.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Article
Engineering, Biomedical
Elora C. Brenneman Wilson, Cheryl E. Quenneville, Monica R. Maly
Summary: The purpose of this study was to integrate MR imaging with full-surface indentation mapping to determine the relationship between femoral cartilage thickness and T2 relaxation change following loading, and cartilage mechanical properties in an ex vivo porcine stifle model. This integrative method shows promise for future work in ex vivo modeling by identifying spatial relationships among variables.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Chemistry, Analytical
Liming Wang, Wulf Amelung, Sabine Willbold
Summary: This study introduces a new method to overcome bias in P-31 nuclear magnetic resonance (NMR) spectra caused by artificial hydrolysis, by using O-18 enriched medium for sample preparation and analysis. The results show that using O-18 enriched medium can accurately differentiate artificial hydrolysis products from natural metabolites, and quantify them accurately. The study also demonstrates that the extent of artificial hydrolysis affecting NMR spectra varies among different types of environmental samples.
ANALYTICAL CHEMISTRY
(2021)
Correction
Biochemical Research Methods
Loraine C. Goenaga-Mafud, Yordania Matos Gamez, Carolina P. Campos, Jose D. Vollet-Filho, Natalia Mayumi Inada, Cristina Kurachi, Vanderlei Salvador Bagnato
Summary: Organ transplantations have a growing medical importance as more individuals are in need of organs. The increase in discarded organs is mainly due to donor infections. This study demonstrates the feasibility of reducing bacterial load in a kidney model using UV-C as a germicidal agent. The results show the effectiveness of this technique.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Florian N. Loch, Carsten Kamphues, Katharina Beyer, Christian Schineis, Patrick Asbach, Rolf Reiter, Ingolf Sack, Juergen Braun
Summary: MRE can be used to assess the viscoelastic properties of healthy and CD-affected ileum. The results showed that the penetration rate was lower, and the damping ratio and viscosity parameter were higher in CD-affected tissue compared to healthy tissue. There was no significant difference in shear wave speed between healthy and diseased tissue.
QUANTITATIVE IMAGING IN MEDICINE AND SURGERY
(2023)
Article
Neurosciences
Karim Gariani, Antoine Klauser, Maria Isabel Vargas, Francois Lazeyras, Christel Tran
Summary: Hyperinsulinism/hyperammonemia syndrome (HI/HA) is caused by activating mutations in the GLUD1 gene, leading to insulin overproduction and chronic asymptomatic hyperammonemia. Brain magnetic resonance imaging in HI/HA patients shows differences in brain biochemistry compared to other hyperammonemia disorders, with normal levels of combined glutamate and glutamine.
Article
Radiology, Nuclear Medicine & Medical Imaging
Julien Songeon, Sebastien Courvoisier, Lijing Xin, Thomas Agius, Oscar Dabrowski, Alban Longchamp, Francois Lazeyras, Antoine Klauser
Summary: This study introduces a new artificial intelligence framework, 31P-SPAWNN, for analyzing phosphorus-31 (P-31) magnetic resonance spectra. The experiment demonstrates the reliability and accuracy of 31P-SPAWNN in estimating metabolite concentrations and spectral parameters. It also shows that 31P-SPAWNN has improved quantification and processing time compared to traditional methods.
MAGNETIC RESONANCE IN MEDICINE
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Oscar Dabrowski, Sebastien Courvoisier, Jean-Luc Falcone, Antoine Klauser, Julien Songeon, Michel Kocher, Bastien Chopard, Francois Lazeyras
Summary: MRI is a non-invasive medical imaging modality that is sensitive to patient motion. This study presents a methodology for acquiring reproducible motion-corrupted MRI images and validating the system's performance through motion estimation using rigid-body volume registration. The proposed protocol is versatile and can potentially benefit the MRI and artificial intelligence research communities.
PHYSICA MEDICA-EUROPEAN JOURNAL OF MEDICAL PHYSICS
(2022)
Article
Transplantation
Thomas Agius, Julien Songeon, Antoine Klauser, Florent Allagnat, Gregoire Longchamp, Raphael Ruttimann, Arnaud Lyon, Julijana Ivaniesevic, Raphael Meier, Sebastien Deglise, James F. Markmann, Korkut Uygun, Leo Buhler, Christian Toso, Jean-Marc Corpataux, Francois Lazeyras, Alban Longchamp
Summary: Subnormothermic ex vivo kidney perfusion improves kidney metabolism, reduces ischemia-reperfusion injuries, and enhances post-transplantation kidney perfusion and histopathological status.
TRANSPLANTATION DIRECT
(2022)
Editorial Material
Medicine, Research & Experimental
Shani Kamberi, Raphael P. H. Meier
Summary: After a pause caused by the COVID-19 pandemic, xenotransplantation literature updates are now restarting. Recent clinical cases and increased interest in the field have prompted the decision to reinstate this section. Pig-to-human xenotransplantation presents unique challenges that will be given specific attention. This issue aims to cover the gap and include the most relevant publications from March 2021 to March 2023.
XENOTRANSPLANTATION
(2023)
Article
Surgery
Charles-Henri Wassmer, Rebecca Revol, Isabelle Uhe, Mickael Chevallay, Christian Toso, Pascal Gervaz, Philippe Morel, Pierre-Alexandre Poletti, Alexandra Platon, Frederic Ris, Frank Schwenter, Thomas Perneger, Raphael P. H. Meier
Summary: A practical clinical severity score was developed and validated to guide the management of patients with small bowel obstruction.
INTERNATIONAL JOURNAL OF SURGERY
(2023)
Article
Multidisciplinary Sciences
Laura Charles, Thomas Agius, Irina Filz von Reiterdank, Janna Hagedorn, Yanis Berkane, Hyshem H. Lancia, Basak E. Uygun, Korkut Uygun, Curtis L. Cetrulo, Mark A. Randolph, Alexandre G. Lellouch
Summary: This paper presents two blood collection procedures for rats: lateral tail vein puncture and dorsal penile vein puncture. These procedures are fast, efficient, and can be used in various situations for blood sampling.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Surgery
Raphael P. H. Meier, Shani Kamberi, Josue Alvarez-Casas, Barton F. Lane, Chandra S. Bhati, Saad Malik, William Twaddell, Kirti Shetty, Adam Fang, Hyun S. Kim, Daniel G. Maluf
PROGRESS IN TRANSPLANTATION
(2023)
Meeting Abstract
Immunology
Yvonne Kelly, Arya Zarinsefat, Raphael Meier, Minnie Sarwal, Peter Stock, Zoltan Laszik, Tara Sigdel
Meeting Abstract
Immunology
Raphael Meier, Miguel Nunez, Shareef Syed, Chris Freise, John Roberts, Nancy Ascher, Ryutaro Hirose, Garrett Roll
Article
Surgery
Raphael Pascal Henri Meier, Yvonne Kelly, Hillary Braun, Daniel Maluf, Chris Freise, Nancy Ascher, John Roberts, Garrett Roll
Summary: This study compares graft survival and biliary complications in liver transplantation using donation-after-circulatory-death, donation-after-brain-death, and living-donation methods. The results show that despite higher rates of biliary complications, donation-after-circulatory-death and living-donation have excellent graft survival.
TRANSPLANT INTERNATIONAL
(2022)
Meeting Abstract
Gastroenterology & Hepatology
Ameer Halim, Parth Thaker, Pei Xue, Kourosh Kalachi, Preet Patel, Madison Parks, Soumya Mishra, Kai Sun, Larry Magder, Nathalie H. Urrunaga, Raphael Meier, Kirti Shetty
Meeting Abstract
Surgery
T. Agius, J. Songeon, A. Klauser, G. Longchamp, F. Allagnat, A. Nastasi, R. Ruttiman, R. P. H. Meier, C. Toso, L. Buehler, J. -M. Corpataux, F. Lazeyras, A. Longchamp
BRITISH JOURNAL OF SURGERY
(2022)