Article
Biophysics
Ting Zheng, Xianming Li, Yanjun Si, Minjin Wang, Yuzhen Zhou, Yusheng Yang, Na Liang, Binwu Ying, Peng Wu
Summary: In this study, an accurate point-of-care testing (POCT) platform was proposed by specific recognition of target amplicons with peptide nucleic acid (PNA, assisted by T7 Exonuclease), which efficiently eliminated false positives and showed high sensitivity and specificity for detecting SARS-CoV-2.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Xiuen Cao, Chuanpin Chen, Qubo Zhu
Summary: This review summarizes the applications of functional nucleic acids and isothermal amplification techniques in biosensing, highlighting their important contributions to the field. These technologies have great potential to enhance the performance of biosensors.
Article
Chemistry, Analytical
Seung Beom Seo, Jin-Seong Hwang, Eunjung Kim, Kyujung Kim, Seokbeom Roh, Gyudo Lee, Jaewoo Lim, Byunghoon Kang, Soojin Jang, Seong Uk Son, Taejoon Kang, Juyeon Jung, Jang-Seong Kim, Keun-Hur, Tae-Su Han, Eun-Kyung Lim
Summary: Researchers have developed a simple and sensitive in vitro diagnostic system for detecting gastric cancer-related biomarkers. The system combines isothermal amplification and lateral flow biosensor to detect target miRNAs with excellent sensitivity and selectivity within 3 hours. This technology is of great importance for the early diagnosis and prognosis monitoring of gastric cancer and can also be applied to detect biomarkers related to other diseases.
Article
Public, Environmental & Occupational Health
Xiaolan Huang, Fei Xiao, Nan Jia, Chunrong Sun, Jin Fu, Zheng Xu, Xiaodai Cui, Hui Huang, Dong Qu, Juan Zhou, Yi Wang
Summary: The ongoing outbreak of monkeypox, caused by monkeypox virus (MPXV), requires a rapid and sensitive MPXV detection method. In this study, a diagnostic test called MPX-LAMP-LFB was developed based on loop-mediated isothermal amplification (LAMP) and nanoparticle-based lateral flow biosensor (LFB) for the diagnosis of MPXV infection. The MPX-LAMP-LFB assay can specifically detect MPXV strains without cross-reaction with non-MPXV pathogens and has a low sensitivity of 5 copies/µl of plasmid template and 12.5 copies/µl of pseudovirus in human blood samples. The entire process of the assay can be completed in about 1 hour, making it a useful tool for rapid and reliable diagnosis of MPXV infection.
FRONTIERS IN PUBLIC HEALTH
(2023)
Review
Food Science & Technology
Jie Zhou, Teng-Yu Wang, Zhi Lan, Han-Jie Yang, Xing-Jian Ye, Rui Min, Zhao-Hui Wang, Qing Huang, Jing Cao, Yu-E Gao, Wen-Long Wang, Xiu-Lan Sun, Yi Zhang
Summary: Foodborne microbial contamination (FMC) is the primary cause of food poisoning and illness. Isothermal amplification methods with functional nucleic acids (FNAs) have enabled the detection of mycotoxins, making biosensors for FMC more sensitive, cost-effective, and less reliant on nanomaterials for signal output.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Analytical
Han Jiang, Qian Wu, Qihong Zhao, Kaiyong Liu, Qingli Bo, Xinsheng Qin, Chao Yan, Lin Huang, Wei Chen, Panzhu Qin
Summary: Foodborne pathogens pose a serious threat to human health worldwide. In this study, a portable lateral flow colorimetric biosensor called CALL was developed using dCas9 as an intermediate bridge to distinguish target amplicons and byproducts. The biosensor showed high specificity and sensitivity in detecting Salmonella, enabling on-site screening of low concentrations in a short time.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Multidisciplinary Sciences
Radhika Biyani, Kirti Sharma, Kenji Kojima, Madhu Biyani, Vishnu Sharma, Tarun Kumawat, Kevin Maafu Juma, Itaru Yanagihara, Shinsuke Fujiwara, Eiichi Kodama, Yuzuru Takamura, Masahiro Takagi, Kiyoshi Yasukawa, Manish Biyani
Summary: This new isothermal nucleic acid amplification assay, called RICCA, allows for rapid and direct detection of viruses with low viral loads. It has shown high sensitivity in detecting RNA viruses directly from saliva samples and synthetic templates of SARS-CoV-2. The assay can be used in real-time point-of-care testing and has a total assay time of 15-30 minutes for detecting heat-inactivated SARS-CoV-2 virus in human saliva.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Xingyun Wang, Guirong Wang, Yacui Wang, Shuting Quan, Hui Qi, Lin Sun, Chen Shen, Hairong Huang, Weiwei Jiao, Adong Shen
Summary: The study developed a simple and reliable method for detecting Mycobacterium tuberculosis complex (MTBC) using a multiplex LAMP-LFB assay, which demonstrated higher sensitivity than traditional culture and Xpert MTB/RIF tests, especially in resource limited settings and probable TB cases.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Hongbo Shan, Yang Wang, Tao Wu, Binwu Ying, Gaolian Xu
Summary: Asymmetric hairpins mediated amplification (HMA) is a novel isothermal amplification method developed for the diagnosis of Mycobacterium Tuberculosis (MTB). It has high sensitivity, specificity, and direct visualization, making it suitable for point-of-care (POC) diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Chemistry, Analytical
Poramin Boonbanjong, Kiatnida Treerattrakoon, Wassa Waiwinya, Piyawat Pitikultham, Deanpen Japrung
Summary: Isothermal amplification (IA) is a nucleic acid amplification technology that has significantly contributed to the healthcare system by improving disease diagnosis through higher sensitivity, specificity, and rapidity. Certain IA techniques provide a rapid, sensitive, specific, and simple method for disease diagnosis. However, not all IA techniques have been widely used in clinical applications due to the influence of different biomarkers and sample types on the IA system.
Article
Microbiology
Xinggui Yang, Junfei Huang, Xu Chen, Ziyu Xiao, Xiaojuan Wang, Yijiang Chen, Wenlin Zheng, Wei Chen, Huijuan Chen, Shiguang Lei, Yong Hu, Shijun Li
Summary: Tuberculosis is a chronic infectious disease mainly caused by Mycobacterium tuberculosis, and the ability to rapidly detect and differentiate MTB is crucial for prevention and treatment. The novel diagnostic method mLAMP-LFB is a rapid, reliable, and sensitive tool that can detect and differentiate MTB, offering potential as a screening tool for tuberculosis.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mao-Ling Sun, Hai-Yun Lai, Na-Yu Chong, Dong-Fan Liu, Zhen-Yi Zhang, Bo Pang, Jun Yao
Summary: A new method for detecting HBV using a combination of MIRA and LFD technologies has been developed, which is simple, rapid, and accurate, making it particularly suitable for resource-limited regions.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Chemistry, Analytical
Ian T. Hull, Enos C. Kline, Gaurav K. Gulati, Jack Henry Kotnik, Nuttada Panpradist, Kamal G. Shah, Qin Wang, Lisa Frenkel, James Lai, Joanne Stekler, Barry R. Lutz
Summary: The study presents a novel quantitative detection method for HIV DNA or RNA using a combination of recombinase polymerase amplification and lateral flow detection technologies, allowing for rapid and accurate testing in low-resource healthcare settings, with the potential to improve the quality of HIV treatment.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Seokhwan Kim, Jung Ho Kim, Seokjoon Kim, Jung Soo Park, Byung Seok Cha, Eun Sung Lee, Jinjoo Han, Jiye Shin, Youngjun Jang, Ki Soo Park
Summary: A loop-mediated isothermal amplification-based nucleic acid lateral flow assay (LAMP-NALFA) system was developed for specific and multiplex detection of genetic markers at a low cost. The system demonstrated high selectivity in discriminating target amplification products and non-specific amplification products, and showed good practical applicability in different samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Ophthalmology
Kui Dong, ZhiMing Kang, Xuan Ji, Xinxin Zhang, PeiNi Cheng, Bin Sun
Summary: The study developed a rapid, specific, and sensitive method for the detection of Pseudomonas aeruginosa, which can be widely utilized in clinical settings.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Junjun Hou, Yan Huang, Lili Fu, Mingzhao Sun, Lingxiao Wang, Runjing Guo, Lingxin Chen, Changjun Lv
Summary: Hydrogen sulfide (H2S), a signaling molecule, plays a beneficial role in lung diseases. However, the changes in endogenous lung H2S levels in chronic pulmonary diseases are unclear. In this study, a fluorescent probe was synthesized for selective imaging of H2S in living cells and mice, and the role of H2S supplementation in the treatment of pulmonary fibrotic diseases was discussed.
ANALYTICAL CHEMISTRY
(2023)
Article
Environmental Sciences
Zhe Wang, Min Lv, Chu-Long Huang, Dong-Dong Zhang, Ruixia Han, Gang Li, Ling-Xin Chen
Summary: This study found that anthropogenic activities, such as urbanization and oil combustion, have a significant impact on the distribution and properties of dissolved organic matter (DOM) in intertidal sediments. The release of organic matters from urbanization and the combustion of oil contribute to the higher concentration of aromatic compounds in the sediments.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Analytical
Jinglong Han, Huajun Liu, Ji Qi, Jiawen Xiang, Longwen Fu, Xiyan Sun, Liyan Wang, Xiaoyan Wang, Bowei Li, Lingxin Chen
Summary: A novel, simple and effective visual fluorescent sensing paper-based chip was developed for the ultrasensitive detection of mercury ions in environmental water. The method has a low detection limit and fast response time, and successfully achieved trace spiking detection of various water sources.
Article
Chemistry, Analytical
Yan Huang, Lili Fu, Junjun Hou, Lingxiao Wang, Mingzhao Sun, Xiao Sun, Xiaoyan Wang, Lingxin Chen
Summary: Researchers designed and synthesized a nanocomposite RCDs@MnO2 to improve the hypoxic environment of tumors and enhance the efficacy of photodynamic therapy. The nanocomposite could generate 1O2 to achieve the purpose of photodynamic therapy and exhibit fluorescence off-on function in tumor cells and tissues. The results showed that RCDs@MnO2 has the potential to be applied in fluorescence imaging and significantly improve the photodynamic therapy of tumors, both in vitro and in vivo.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Qian Yu, Hoa Duc Trinh, Yeonji Lee, Taejoon Kang, Lingxin Chen, Sangwoon Yoon, Jaebum Choo
Summary: In this study, a silica-encapsulated Au core-satellite (CS@SiO2) nanotag was developed, which exhibited strong and reproducible surface-enhanced Raman scattering (SERS) signal. By combining SERS with enzyme-linked immunosorbent assay (ELISA), a highly sensitive detection of SARS-CoV-2 was achieved. The CS@SiO2 nanotags showed superior performance with a detection limit of 8.81 PFU mL-1, which is 10 times better than conventional ELISA and 100 times better than lateral flow assay strip method. The new CS@SiO2-SERS-ELISA method can significantly reduce the false-negative diagnostic rate for SARS-CoV-2 and contribute to overcoming the current pandemic crisis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Haojie Cong, Hao Ding, Guoqing Wang, Xiaokun Wang, Lingxin Chen
Summary: In this study, an on-site hypoxanthine (Hx) sensing method was designed using manganese dioxide (MnO2) nanosheets to detect both hydrogen peroxide (H2O2) and uric acid (UA). The proposed method exhibited high sensitivity towards Hx and was successfully used to monitor actual sample spoilage under different storage conditions. A WeChat mini program was developed for on-site evaluation.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Yixuan Wu, Qian Yu, Younju Joung, Chang Su Jeon, Seunghyun Lee, Sung Hyun Pyun, Sang-Woo Joo, Lingxin Chen, Jaebum Choo
Summary: This study reports the development of a reproducible and highly sensitive surface-enhanced Raman scattering (SERS) substrate using a butanol-induced self-assembly of gold nanoparticles (AuNPs) and its application as a rapid diagnostic platform for SARS-CoV-2. The substrate showed strong electromagnetic enhancement of the Raman signals, enabling the detection of SARS-CoV-2 with a very low limit of detection (LoD) of 3.1 x 10(-15) M. This method provides sufficient sensitivity for SARS-CoV-2 screening assay and reduces the diagnostic time significantly. The results suggest the potential of this approach for developing rapid diagnostic platforms for other biomolecules and infectious diseases.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Ji Qi, Bowei Li, Dong Lin, Zhiqiang Du, Longwen Fu, Xiaoyan Wang, Zhiyang Zhang, Liqiang Luo, Lingxin Chen
Summary: To overcome the distortion of fluorescent images by mobile devices, a dual-mode strategy was developed to achieve undistorted visual fluorescent sensing on & mu;PAD by manipulating the coffee-ring effect of the fluid sample. By dividing the resulting fluorescence image into 600 pixels and using a fluorescent probe, accurate quantitative information was obtained to avoid image distortion. This strategy, combined with a small imaging box and a smartphone, allows for rapid testing of histidine in human urine and improved antidistortion for visual fluorescent sensing.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Liyan Bi, Huangruici Zhang, Wenchao Hu, Jiadong Chen, Yixuan Wu, Hao Chen, Bingqian Li, Zhiyang Zhang, Jaebum Choo, Lingxin Chen
Summary: In this study, a novel M13 phage-based SERS nanocarrier was developed for the detection and inactivation of E. coli. The M13 phage was used as a biological scaffold to conjugate with Au@Ag core-shell nanorods (Au@AgNR) and specifically target E. coli. The proposed SERS probe showed a detection limit of 0.5 cfu/mL and high antibacterial efficiency of approximately 90% against E. coli.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Engineering, Environmental
Yinan Li, Jianxin Fu, Lin Peng, Xun Sun, Guoqing Wang, Yunqing Wang, Lingxin Chen
Summary: The extensive occurrence of small plastic particles in the environment and our foods, and the resulting hazardous effects to nature and people have become a global concern. A new strategy using an olive oil-based emulsion has been developed to efficiently remove microplastics and nanoplastics. This emulsion can adsorb onto plastic particles and successfully remove them from seawater, tea leaves, soil, and toothpaste.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Environmental
Liyan Wang, Jialuo Yu, Xiaoyan Wang, Jinhua Li, Lingxin Chen
Summary: Environment pollution is a global issue that requires high-performance adsorbent materials for effective removal and remediation. Nanocomposites have emerged as a powerful tool for pollutant removal due to their superior adsorption performances. Among them, molecular imprinting-based nanocomposites with tailor-made binding sites have drawn great research interests in the field of environmental pollutants removal. This article provides a summary of the general fabrication and typical components of molecular imprinting-based nanocomposite adsorbents and discusses synthesis strategies for enhancing the adsorption performances, serving as a background for future research.
JOURNAL OF HAZARDOUS MATERIALS LETTERS
(2023)
Article
Environmental Sciences
Suyu Ren, Xiaojie Jin, Tadiyose Girma Bekele, Min Lv, Jing Ding, Feng Tan, Lingxin Chen
Summary: This study developed a reliable sampling approach, using DGT with XAD-2 as the binding agent, for monitoring 4-MBC in coastal waters. The diffusion coefficients of 4-MBC in freshwater and artificial seawater were determined and the XAD-2 binding gel showed rapid adsorption to 4-MBC. Field deployment showed consistent results with grab sampling, and the DGT method is suitable for seasonal monitoring, source identification, and risk assessment of 4-MBC in coastal waters.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Chemistry, Analytical
Yuhao Wen, Dani Sun, Yue Zhang, Zhong Zhang, Lingxin Chen, Jinhua Li
Summary: Environmental protection and food safety are important for human society, and there is a need for analytical methods to detect pollutants in food. Molecular imprinting-based ratiometric fluorescence (MI-RFL) sensors, with advantages such as selectivity, sensitivity, and convenience, have gained interest in analytical determination. This review summarizes recent advances in MI-RFL sensors for environmental and food analysis, including fluorescence sources, sensing principles, new imprinting techniques, and analytical applications. Challenges and perspectives for improving sensitivity/visualization and extending applications are also discussed.
Article
Materials Science, Biomaterials
Xiaoyue Han, Yanlong Xing, Xinyu Song, Kun Dou, Fabiao Yu, Lingxin Chen
Summary: This study developed a fluorescent probe called CyO-Disu for specific sensing of glutathione (GSH) variation in live cells and mice models, providing direct evidence of GSH concentration changes during the progression of hepatocellular carcinoma (HCC). The probe could potentially serve as a powerful tool for early diagnosis and precise treatment of HCC using GSH as a reliable biomarker.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Engineering, Environmental
Jing Ding, Zhaoqin Liang, Min Lv, Xiuyu Li, Shuang Lu, Suyu Ren, Xiaoyong Yang, Xiaoqiang Li, Chen Tu, Dong Zhu, Lingxin Chen
Summary: This study investigates the effects of soil aging on the toxicity of microplastics to soil fauna. The results show that soil aging slightly alters the surface properties and morphology of microplastics, and significantly affects the release of additives. Additionally, a less diverse microbial community is formed on the surface of microplastics compared to the surrounding soils. Aged microplastics have a significant impact on the reproduction of soil worms, particularly those aged in soil with manure, and disrupt their gut microbiota. Changes in biofilm features and leaching properties of microplastics during aging are the main contributing factors to these shifts. This study provides preliminary insights into the role of soil aging in the toxicity of microplastics to soil fauna.
JOURNAL OF HAZARDOUS MATERIALS
(2024)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).