期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 313, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.128017
关键词
Neurotransmitters; Microfluidic separation and sensing; Screen printed device; Alternating current induced separation; Electrochemical sensing; Evans blue
资金
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [2019R1A2C1002531]
- Australia-Korea Foundation
- National Research Foundation of Korea [2019R1A2C1002531] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A novel electrochemical microfluidic separation and sensing (EMSS) device was fabricated for monitoring neurotransmitters (NTs) in human blood plasma. Initially, the neurotransmitter separation was achieved with the AC field perturbation in a microfluidic channel followed by the successive detection using a sensor at the end of channel. The sensor performances using some of electron transfer mediators were demonstrated for sensing of seven NTs (5-hydroxytryptamine, dopamine, norepinephrine, epinephrine, 5-hydroxyindoleacetic acid, 5-hydroxytryptophan, and 3, 4-dihydroxy-l-phenylalanine). The sensor probe was fabricated by electropolymerization of 2, 2 ':5 ', 5 ''-terthiophene-3 '-p-benzoic acid (TBA) with heteroatoms (N and S) doped porous carbon (HPC) to form a composite layer onto the screen printed carbon electrode, followed by covalent immobilization of a redox mediator. Among the tested mediators, Evans blue revealed the most superior performance. The parameters affecting the sensing performance were optimized, in terms of pH, temperature, fluid flow rate and AC parameters (i.e., frequency, amplitude, etc). The calibration plots for the seven NTs standard samples were found to be linear within the range of 0.05-130 nM with the detection limits between 0.034 (+/- 0.005) and 0.044 (+/- 0.004) nM. Finally, the reliability of the proposed device was assessed by detecting six NTs and two NTs derivatives in a human plasma sample.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据