期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 312, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.127987
关键词
Ternary nanocube; Electrochemiluminescence; Pb2+-trequiring DNAzyme; Ferrocene; HBV-related DNA
资金
- National Science and Technology Major Project of the Ministry of Science and Technology of China [2018ZX10732202]
- Postgraduate scientific research innovation project of Chongqing [CYS19216]
- National Natural Science Foundation of China [81972023]
- Science and Technology Research Project of Chongqing Education Commission [KJQN201900425]
Hepatitis B virus (HBV) infection remains a global public health problem, which is directly associated with a very high risk of evolving into liver cirrhosis and hepatocellular carcinoma (HCC). So far, enzyme-linked immunosorbent assay (ELISA) is still a main detection method for HBV infection in hospital, by which it enables to identify HBV infection in vitro through detection of circulating hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg). However, ELISA requires antibody-dependent and enzyme-labeling. What's worse, ELISA is silent to the type of occult HBV infection, which does not express HBsAg. Thus, it is of significance to establish a favorable alternative to detect HBV-related specific DNA rather than proteins, as well as to avoid antibody and enzyme-labeling assay for unambiguous screening of HBV infection. Herein, we have exploited a ternary nanocube (AgAuPt NCs)-based off-on blinking-type electrochemiluminescence (ECL) biosensor towards detection of HBV-related DNA for the first time, integrated with Pb2+-requiring DNAzyme recycling amplification. By this way, the detection limit down to 65 aM is achieved. The unique off-on blinking-type ECL biosensor not only overcomes the drawbacks of ELISA towards detection for HBV infection but also provides a potential for clinical application.
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