Enhanced biodegradation of an endocrine disrupting micro-pollutant: Di (2-ethylhexyl) phthalate using biogenic self-assembled monolayer of silver nanoparticles

Di (2-ethylhexyl) phthalate (DEHP) is one of the predominant plasticizer and an endocrine disrupting chemical occurring almost in all partitions of the environment. Though DEHP occur at lower concentration, reluctance arises due to their ability to disrupt endocrine system even lower concentration. In the present study, DEHP was assessed for degradation at minimal level (1-100 mu g L-1) by a novel bacterial strain, Rhodococcus jostii PEVJ9. In the experimental design, significant variables were concentration of silver nitrate and DEHP, pH, temperature, time and agitation. Degradation without SAM-silver nanoparticles was 30-66% (predicted value = 30.8-66.8%, R-2 = 99.7%) while, degradation in the presence of SAM-silver nanoparticles onto bacterial cells was 100% (predicted value = 98.4-102.1%, R-2 99.6%) within 72 h. In short, this is the first report illustrating the experimental designs in biogenic synthesis of SAM-silver nanoparticles and enhanced degradation of DEHP at minimal level. The study overcomes poor bioavailability and assimilation of DEHP at lower concentration by the microbial population present in the environment. Thus, an efficient clean-up would prevent or minimize DEHP exposure at all trophic levels ranging from feminization of fishes to reproductive disorders in humans. (C) 2020 Elsevier B.V. All rights reserved.