期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 117, 期 28, 页码 16649-16659出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2000926117
关键词
arbuscular mycorrhiza; RNA sequencing; nitrate transporter; nitrogen uptake; OsNPF4.5
资金
- National Key Research and Development Program of China [2016YFD0100700]
- National Natural Science Foundation of China [31572188, 31372121]
- Basic Research Program of Jiangsu province in China [BK20181324]
- Innovative Research Team Development Plan of the Ministry of Education of China [IRT_17R56, KYT201802]
- Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization
Low availability of nitrogen (N) is often a major limiting factor to crop yield in most nutrient-poor soils. Arbuscular mycorrhizal (AM) fungi are beneficial symbionts of most land plants that enhance plant nutrient uptake, particularly of phosphate. A growing num-ber of reports point to the substantially increased N accumulation in many mycorrhizal plants; however, the contribution of AM symbiosis to plant N nutrition and the mechanisms underlying the AM-mediated N acquisition are still in the early stages of being understood. Here, we report that inoculation with AM fungus Rhizophagus irregularis re-markably promoted rice (Oryza sativa) growth and N acquisition, and about 42% of the overall N acquired by rice roots could be delivered via the symbiotic route under N-NO3- supply condition. Mycorrhizal colonization strongly induced expression of the pu-tative nitrate transporter gene OsNPF4.5 in rice roots, and its orthologs ZmNPF4.5 in Zea mays and SbNPF4.5 in Sorghum bi-color. OsNPF4.5 is exclusively expressed in the cells containing arbuscules and displayed a low-affinity NO3- transport activity when expressed in Xenopus laevis oocytes. Moreover, knockout of OsNPF4.5 resulted in a 45% decrease in symbiotic N uptake and a significant reduction in arbuscule incidence when NO3- was supplied as an N source. Based on our results, we propose that the NPF4.5 plays a key role in mycorrhizal NO3- acquisition, a symbiotic N uptake route that might be highly conserved in gramineous species.
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