Nonspecific cleavages arising from reconstitution of trypsin under mildly acidic conditions
出版年份 2020 全文链接
标题
Nonspecific cleavages arising from reconstitution of trypsin under mildly acidic conditions
作者
关键词
Serine proteases, Acetic acid, Signal peptides, Database searching, Autolysis, Specimen storage, Vendors, Synthetic biotherapeutics
出版物
PLoS One
Volume 15, Issue 7, Pages e0236740
出版商
Public Library of Science (PLoS)
发表日期
2020-07-29
DOI
10.1371/journal.pone.0236740
参考文献
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注意:仅列出部分参考文献,下载原文获取全部文献信息。- Reliable LC-MS Multi-Attribute Method for Biotherapeutics by Run-Time Response Calibration
- (2019) Zhongqi Zhang et al. ANALYTICAL CHEMISTRY
- An Automated and Qualified Platform Method for Site-Specific Succinimide and Deamidation Quantitation Using Low-pH Peptide Mapping
- (2019) Mingyan Cao et al. JOURNAL OF PHARMACEUTICAL SCIENCES
- Enhanced trypsin on a budget: Stabilization, purification and high-temperature application of inexpensive commercial trypsin for proteomics applications
- (2019) Søren Heissel et al. PLoS One
- Protein Footprinting and X-ray Crystallography Reveal the Interaction of PD-L1 and a Macrocyclic Peptide
- (2019) Ben Niu et al. BIOCHEMISTRY
- Development of an LC-MS/MS peptide mapping protocol for the NISTmAb
- (2018) Trina Mouchahoir et al. ANALYTICAL AND BIOANALYTICAL CHEMISTRY
- The NISTmAb Reference Material 8671 value assignment, homogeneity, and stability
- (2018) John E. Schiel et al. ANALYTICAL AND BIOANALYTICAL CHEMISTRY
- Pseudotrypsin: A Little-Known Trypsin Proteoform
- (2018) Zdeněk Perutka et al. MOLECULES
- Controlling nonspecific trypsin cleavages in LC-MS/MS-based shotgun proteomics using optimized experimental conditions
- (2015) Pan Fang et al. ANALYST
- Development of a quantitative mass spectrometry multi-attribute method for characterization, quality control testing and disposition of biologics
- (2015) Richard S Rogers et al. mAbs
- Evaluation of Pseudotrypsin Cleavage Specificity Towards Proteins by MALDI-TOF Mass Spectrometry
- (2015) Filip Dycka et al. PROTEIN AND PEPTIDE LETTERS
- Quantitative Bottom-Up Proteomics Depends on Digestion Conditions
- (2013) Mark S. Lowenthal et al. ANALYTICAL CHEMISTRY
- Understanding the Role of Proteolytic Digestion on Discovery and Targeted Proteomic Measurements Using Liquid Chromatography Tandem Mass Spectrometry and Design of Experiments
- (2013) Philip L. Loziuk et al. JOURNAL OF PROTEOME RESEARCH
- Improving Peptide Identification Sensitivity in Shotgun Proteomics by Stratification of Search Space
- (2013) Gelio Alves et al. JOURNAL OF PROTEOME RESEARCH
- Protein Digestion: An Overview of the Available Techniques and Recent Developments
- (2013) Linda Switzar et al. JOURNAL OF PROTEOME RESEARCH
- Getting intimate with trypsin, the leading protease in proteomics
- (2013) Elien Vandermarliere et al. MASS SPECTROMETRY REVIEWS
- In-Source Fragmentation and the Sources of Partially Tryptic Peptides in Shotgun Proteomics
- (2012) Jong-Seo Kim et al. JOURNAL OF PROTEOME RESEARCH
- Microscale affinity purification of trypsin reduces background peptides in matrix-assisted laser desorption/ionization mass spectrometry of protein digests
- (2011) Ivo Chamrád et al. Journal of Proteomics
- Systematic and quantitative comparison of digest efficiency and specificity reveals the impact of trypsin quality on MS-based proteomics
- (2011) Julia Maria Burkhart et al. Journal of Proteomics
- Taking Advantage of Nonspecific Trypsin Cleavages for the Identification of Seed Storage Proteins in Cereals
- (2009) Kjell Sergeant et al. JOURNAL OF PROTEOME RESEARCH
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