Article
Plant Sciences
Saad Sulieman, Mostafa Abdelrahman, Lam-Son Phan Tran
Summary: This review discusses the carbon metabolic changes in soybean nodules under phosphate stress, including strategies such as regulating carbon partitioning, modifying respiratory pathways, and exporting nitrogen products. However, there is significant genetic variation in nodule acclimation to phosphate stress depending on crop genotypes and rhizobial strains in symbiosis.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Cell Biology
Klara Kaleb, Victor Pedrosa, Claudia Clopath
Summary: Despite the stability of neural activity, the aspects conserved and how flexibility for learning and memory is maintained remain not fully understood. Experimental studies suggest the existence of network-centered control in addition to neuron-centered control. Input-dependent inhibitory plasticity (IDIP) can explain various phenomena and provide diverse functional implications in establishing global network balance.
Article
Multidisciplinary Sciences
Xiaoshuang Wei, Yu Fu, Renjie Yu, Lei Wu, Zhihai Wu, Ping Tian, Siyuan Li, Xue Yang, Meiying Yang
Summary: This study identified 57 members of the GmPHTs family in soybean and investigated their evolutionary relationships and chromosome duplications. Promoter analysis revealed the presence of light-responsive and abiotic stress-responsive elements in the GmPHT genes. Additionally, GmPHT gene expression showed different patterns in response to low phosphorus and drought stress.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Dusan Velickovic, Yen-Chen Liao, Stephanie Thibert, Marija Velickovic, Christopher Anderton, Josef Voglmeir, Gary Stacey, Mowei Zhou
Summary: In this study, an enzyme-assisted matrix-assisted laser desorption/ionization mass spectrometry imaging strategy was used to visualize the distribution and identity of N-glycans in soybean root nodules. Ion images showed that N-glycosylation mainly occurred in the sclerenchyma layer and infected cells within the infection zone, and mutant nodules had more abundant N-glycan structures. Proteomic analysis revealed that these glycans likely played a role in nitrogen fixation and phenylpropanoid biosynthesis.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Yubo Yao, Hongmei Yuan, Guangwen Wu, Jun Yan, Dongsheng Zhao, Si Chen, Qinghua Kang, Chunmei Ma, Zhenping Gong
Summary: Phosphorus is necessary for nitrogen fixation in soybean root nodules. Phosphorus stress inhibits nitrogen accumulation, quantity, weight, SNA, and ARA in root nodules, as well as affects the expression of associated metabolic pathways. Flavonoids and ascorbate and aldarate metabolism play important roles in regulating nodulation and nitrogen fixation under phosphorus stress.
Article
Biochemistry & Molecular Biology
Zhiyuan Cheng, Xiaomei Zhang, Penghui Huang, Guowen Huang, Jinglong Zhu, Fulu Chen, Yuchen Miao, Liangyu Liu, Yong-Fu Fu, Xu Wang
Article
Plant Sciences
Long Xiao, Shanshan Jiang, Penghui Huang, Fulu Chen, Xu Wang, Zhiyuan Cheng, Yuchen Miao, Liangyu Liu, Iain Searle, Chunyan Liu, Xiao-Xia Wu, Yong-Fu Fu, Qingshan Chen, Xiao-Mei Zhang
Article
Biotechnology & Applied Microbiology
Yingxin Zhang, Chengming Fan, Yuhong Chen, Richard R. C. Wang, Xiangqi Zhang, Fangpu Han, Zanmin Hu
Summary: The study revealed specific distribution patterns of SSRs in different chromosomes from different species, providing efficient visible markers for identification of individual chromosomes and tracing SSR sequence evolution from diploid progenitors to hexaploid wheat. The greatest changes in SSRs, including (AAC)(n), (AAG)(n), (AGC)(n), and (AG)(n), were observed in the B genome during wheat evolution, especially in centromeric and pericentromeric regions, suggesting that the B genome might be more sensitive to genome shock and more changeable during wheat polyploidization.
Article
Biotechnology & Applied Microbiology
Xiao Liu, Dan Zhang, Jianhui Zhang, Yuhong Chen, Xiuli Liu, Chengming Fan, Richard R-C. Wang, Yongyue Hou, Zanmin Hu
Summary: Microalgae are considered a highly promising source for biodiesel production, and this study successfully increased lipid content in Chlorella ellipsoidea by transferring the transcription factor LEC1 from Arabidopsis thaliana. The significant changes in gene expression, especially key genes in the lipid synthesis pathway, did not affect growth rate and biomass. Transcriptomic analysis revealed a large number of genes with altered expression in the transgenic strain, including many related to lipid metabolism.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Kun Xu, Xiao-Mei Zhang, Haifeng Chen, Chanjuan Zhang, Jinlong Zhu, Zhiyuan Cheng, Penghui Huang, Xinan Zhou, Yuchen Miao, Xianzhong Feng, Yong-Fu Fu
Summary: Through screening transgenic lines with RNAi-silenced florigen homologs in soybean, a line named GmFTL-RNAi#1 was identified, which showed minor changes in expression and flowering time but significant increase in soybean yield. This demonstrates a potential trade-off between flowering time and high yield by fine-tuning the expression of flowering activators.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Xiangzhen Yin, Xupeng Guo, Lizong Hu, Shuangshuang Li, Yuhong Chen, Jingqiao Wang, Richard R-C Wang, Chengming Fan, Zanmin Hu
Summary: This study extensively evaluated the DGAT enzymes in Brassica napus, revealing significant differences in function and structure among different types of DGATs. Overexpressing BnaDGAT1 in yeast promotes lipid synthesis, indicating functional divergence among different BnaDGAT subfamilies.
Article
Biochemistry & Molecular Biology
Penghui Huang, Mingyang Lu, Xiangbei Li, Huiyu Sun, Zhiyuan Cheng, Yuchen Miao, Yongfu Fu, Xiaomei Zhang
Summary: The stable genetic transformation of soybean is time-consuming and inefficient. To address this issue, researchers developed a new efficient method for regeneration and transformation of hairy roots in soybean, achieving high efficiency within a shorter period.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mingxia Zhang, Rui Dong, Penghui Huang, Mingyang Lu, Xianzhong Feng, Yongfu Fu, Xiaomei Zhang
Summary: We identified a novel gene named NSS in soybeans that is involved in seed development. The S006 mutant, a random mutant of the GmFTL4pro:GUS transgenic line, exhibited small and brown seed coats. Metabolomics and transcriptome analysis revealed that the brown coat was due to increased expression of chalcone synthase 7/8 genes, while down-regulated expression of NSS resulted in small seed size. The function of NSS was confirmed through microscopic observation of the seed-coat integument cells in a CRISPR/Cas9-edited nss1 mutant.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Penghui Huang, Xiaomei Zhang, Zhiyuan Cheng, Xu Wang, Yuchen Miao, Guowen Huang, Yong-Fu Fu, Xianzhong Feng
Summary: The nuclear pore complex Y-complex plays a crucial role in regulating gene expression and flowering in Arabidopsis by altering histone modifications and facilitating transcription at the FLOWERING LOCUS C locus.