期刊
PLANT BIOLOGY
卷 22, 期 6, 页码 1072-1085出版社
WILEY
DOI: 10.1111/plb.13155
关键词
Leaf senescence; phytohormones; transcription factor; wheat; WRKY
资金
- National Natural Science Foundation of China [31671694, 31871616, 31600227, 31970199]
- Major Basic Research Program of Hebei Province [18966337D]
- HAAFS Agriculture Science and Technology Innovation Project [2019-4-8-1]
- China Postdoctoral Science Foundation [2017M621096]
- Support Program for Hundreds of Outstanding Innovative Talents in Higher Education Institutions of Hebei Province (III) [SLRC2017043]
Leaf senescence is a complex and precise regulatory process that is correlated with numerous internal and environmental factors. Leaf senescence is tightly related to the redistribution of nutrients, which significantly affects productivity and quality, especially in crops. Evidence shows that the mediation of transcriptional regulation by WRKY transcription factors is vital for the fine-tuning of leaf senescence. However, the underlying mechanisms of the involvement of WRKY in leaf senescence are still unclear in wheat. Using RNA sequencing data, we isolated a novel WRKY transcription factor, TaWRKY40-D, which localizes in the nucleus and is basically induced by the progression of leaf senescence. TaWRKY40-D is a promoter of natural and dark-induced leaf senescence in transgenicArabidopsis thalianaand wheat. We also demonstrated a positive response ofTaWRKY40-Din wheat upon jasmonic acid (JA) and abscisic acid (ABA) treatment. Consistent with this, the detached leaves ofTaWRKY40-DVIGS (virus-induced gene silencing) wheat plants showed a stay-green phenotype, whileTaWRKY40-DoverexpressingArabidopsisplants showed premature leaf senescence after JA and ABA treatment. Moreover, our results revealed that TaWRKY40-D positively regulates leaf senescence, possibly by altering the biosynthesis and signalling of JA and ABA pathway genes. Together, our results suggest a new regulator of JA- and ABA-related leaf senescence, as well as a new candidate gene that can be used for molecular breeding in wheat.
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