Article
Chemistry, Analytical
Xiaoke Bi, Connor Beck, Yiyang Gong
Summary: Genetically encoded fluorescent indicators, combined with optical imaging, have improved the detection of physiologically or behaviorally relevant neural activity with high spatiotemporal resolution. Protein engineering and screening strategies have enhanced the properties of genetically encoded fluorescent indicators, detecting neurotransmitter and calcium dynamics with high signal-to-noise ratio both in vitro and in vivo.
Article
Multidisciplinary Sciences
Tianchen Wu, Manoj Kumar, Jing Zhang, Shengyu Zhao, Mikhail Drobizhev, Mason McCollum, Charles T. Anderson, Ying Wang, Antje Pokorny, Xiaodong Tian, Yiyu Zhang, Thanos Tzounopoulos, Hui-wang Ai
Summary: A genetically encoded far-red fluorescent indicator, FRISZ, was developed to monitor synaptic Zn2+ dynamics in the nervous system. The indicator was anchored to the mammalian extracellular membrane and successfully used to image synaptic Zn2+ in brain slices and awake mice. This study establishes a new technology for studying the roles of synaptic Zn2+ in the nervous system.
Article
Multidisciplinary Sciences
Liang Wang, Chunling Wu, Wanling Peng, Ziliang Zhou, Jianzhi Zeng, Xuelin Li, Yini Yang, Shuguang Yu, Ye Zou, Mian Huang, Chang Liu, Yefei Chen, Yi Li, Panpan Ti, Wenfeng Liu, Yufeng Gao, Wei Zheng, Haining Zhong, Shangbang Gao, Zhonghua Lu, Pei-Gen Ren, Ho Leung Ng, Jie He, Shoudeng Chen, Min Xu, Yulong Li, Jun Chu
Summary: In this study, a new fluorescent indicator called G-Flamp1 was developed to accurately measure levels of cAMP in living cells. G-Flamp1 exhibited good brightness, appropriate affinity, and fast response kinetics. The results showed that G-Flamp1 enables sensitive monitoring of endogenous cAMP signals in living organisms such as fruit flies and mice.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Rainer Waadt, Joerg Kudla, Hannes Kollist
Summary: Understanding plant physiology at the organismic scale involves analyzing biological processes down to the cellular and molecular levels. Fluorescent protein (FP) technology allows for direct monitoring of various cellular dynamics in plants, and multiplexing techniques enable simultaneous recording of multiple biological processes.
Article
Biochemical Research Methods
Eric D. Petersen, Alexandra P. Lapan, E. Alejandro Castellanos Franco, Adam J. Fillion, Emmanuel L. Crespo, Gerard G. Lambert, Connor J. Grady, Albertina T. Zanca, Richard Orcutt, Ute Hochgeschwender, Nathan C. Shaner, Assaf A. Gilad
Summary: Genetically encoded bioluminescent neurotransmitter indicators have been developed and optimized by high-throughput screening. These indicators can accurately report the presence of the excitatory neurotransmitter glutamate. This new approach allows for the engineering of specific bioluminescent probes for multiple neurotransmitters in the future.
ACS SYNTHETIC BIOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Minji Wang, Yifan Da, Yang Tian
Summary: Genetically encoded fluorescent sensors are powerful tools for visualizing physiological processes in living cells and animals. This study reviews the history and structural basis of fluorescent proteins and discusses the design of genetically encoded biosensors. Several major types of genetically encoded biosensors currently used are also briefly reviewed, providing insight for future fluorescent biosensor design.
CHEMICAL SOCIETY REVIEWS
(2023)
Review
Biochemistry & Molecular Biology
Julian Day-Cooney, Rochelin Dalangin, Haining Zhong, Tianyi Mao
Summary: This article summarizes the recent progress in the development and application of genetically encoded fluorescent sensors, highlighting their use in in vivo imaging. These new technologies enable neuroscientists to monitor and study various aspects of brain activity, advancing our understanding of brain function.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Mei Hong Zhu, Jinyoung Jang, Milena M. Milosevic, Srdjan D. Antic
Summary: Genetically-encoded calcium indicators (GECIs) and voltage indicators (GEVIs) have different strengths and weaknesses in measuring neuronal activity, leading to potential misleading imaging results. GECI imaging signals have better signal-to-noise ratio compared to GEVI signals, but they also attenuate more with distance from the stimulation site. Additionally, GECI imaging can exaggerate temporal summation effects, leading to misinterpretations of neuronal activity.
SCIENTIFIC REPORTS
(2021)
Article
Pharmacology & Pharmacy
Wenfeng Liu, Chang Liu, Pei-Gen Ren, Jun Chu, Liang Wang
Summary: In this study, improved variants of the cAMP indicator G-Flamp1, namely G-Flamp2 and G-Flamp2b, were developed. G-Flamp2 showed increased fluorescence intensity and larger fluorescence changes compared to G-Flamp1, while G-Flamp2b exhibited increased fluorescence intensity but smaller fluorescence changes. Live cell imaging with G-Flamp2 confirmed the ability of cytosolic cAMP to enter the mitochondrial matrix and also revealed activation of the orphan GPCR GPR50 by adipose tissue extract in HEK293T cells.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Oksana M. Subach, Natalia V. Barykina, Elizaveta S. Chefanova, Anna V. Vlaskina, Vladimir P. Sotskov, Olga I. Ivashkina, Konstantin V. Anokhin, Fedor V. Subach
Summary: The development of red fluorescent genetically encoded calcium indicators (GECIs) has expanded the range of colors available for visualizing neuronal calcium activity in vivo, with the newly developed FRCaMP showing higher calcium affinity. In cellular experiments, FRCaMP exhibited similar performance to R-GECO1, and could robustly visualize neuronal activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Hyunbin Kim, In-Yeop Baek, Jihye Seong
Summary: This review introduces genetically encoded fluorescent biosensors that can precisely monitor the real-time activation process of G protein-coupled receptors (GPCRs) in live cells. These sensors allow observation of the activation process of GPCRs, including the binding of extracellular ligands, conformational changes of GPCRs, recruitment of G proteins or beta-arrestin, internalization and trafficking of GPCRs, and downstream signaling events associated with GPCRs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Vera S. Ovechkina, Suren M. Zakian, Sergey P. Medvedev, Kamila R. Valetdinova
Summary: Genetically encoded fluorescent biosensors allow visualization of biological processes in real time, providing benefits such as monitoring drug action and screening at the single-cell resolution. Research aims to improve biomedical applications by exploring their potential and addressing challenges.
Article
Biochemical Research Methods
Gerard Joey Broussard, Leopoldo Petreanu
Summary: This article discusses the observation of axonal terminal activity using genetically encoded calcium sensors and various scales of axonal recording methods. The challenges associated with imaging approaches in the axonal compartment are defined and the latest methodological advances for addressing these issues are highlighted. Furthermore, the potential application of new technologies in conjunction with axon calcium imaging to address current questions in neurobiology is explored.
JOURNAL OF NEUROSCIENCE METHODS
(2021)
Review
Chemistry, Medicinal
Yu Pang, Hao Zhang, Hui-wang Ai
Summary: Redox-active molecules play crucial roles in cell processes, and dysregulation can lead to diseases. Genetically encoded redox indicators are valuable tools for monitoring redox states.
CHEMICAL RESEARCH IN TOXICOLOGY
(2021)
Article
Multidisciplinary Sciences
Yusuke Nasu, Ciaran Murphy-Royal, Yurong Wen, Jordan N. Haidey, Rosana S. Molina, Abhi Aggarwal, Shuce Zhang, Yuki Kamijo, Marie-Eve Paquet, Kaspar Podgorski, Mikhail Drobizhev, Jaideep S. Bains, M. Joanne Lemieux, Grant R. Gordon, Robert E. Campbell
Summary: L-Lactate, traditionally considered a metabolic waste product, is now recognized as an important intercellular energy currency in mammals. A genetically encoded biosensor called eLACCO1.1 has been developed to enable cellular resolution imaging of extracellular L-lactate in cultured mammalian cells and brain tissue.
NATURE COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Christina K. Kim, Samuel J. Yang, Nandini Pichamoorthy, Noah P. Young, Isaac Kauvar, Joshua H. Jennings, Talia N. Lerner, Andre Berndt, Soo Yeun Lee, Charu Ramakrishnan, Thomas J. Davidson, Masatoshi Inoue, Haruhiko Bito, Karl Deisseroth
Review
Biochemistry & Molecular Biology
Sayaka Takemoto-Kimura, Kanzo Suzuki, Shin-Ichiro Horigane, Satoshi Kamijo, Masatoshi Inoue, Masayuki Sakamoto, Hajime Fujii, Haruhiko Bito
JOURNAL OF NEUROCHEMISTRY
(2017)
Article
Cell Biology
Aslihan Selimbeyoglu, Christina K. Kim, Masatoshi Inoue, Soo Yeun Lee, Alice S. O. Hong, Isaac Kauvar, Charu Ramakrishnan, Lief E. Fenno, Thomas J. Davidson, Matthew Wright, Karl Deisseroth
SCIENCE TRANSLATIONAL MEDICINE
(2017)
Article
Physiology
Kyo Koizumi, Masatoshi Inoue, Srikanta Chowdhury, Haruhiko Bito, Akihiro Yamanaka, Toru Ishizuka, Hiromu Yawo
JOURNAL OF PHYSIOLOGICAL SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
So-ichi Tamai, Keisuke Imaizumi, Nobuhiro Kurabayashi, Minh Dang Nguyen, Takaya Abe, Masatoshi Inoue, Yoshitaka Fukada, Kamon Sanada
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Biochemical Research Methods
Masatoshi Inoue, Atsuya Takeuchi, Shin-ichiro Horigane, Masamichi Ohkura, Keiko Gengyo-Ando, Hajime Fujii, Satoshi Kamijo, Sayaka Takemoto-Kimura, Masanobu Kano, Junichi Nakai, Kazuo Kitamura, Haruhiko Bito
Article
Biochemistry & Molecular Biology
Masatoshi Inoue, Atsuya Takeuchi, Satoshi Manita, Shin-ichiro Horigane, Masayuki Sakamoto, Ryosuke Kawakami, Kazushi Yamaguchi, Kouhei Otomo, Hiroyuki Yokoyama, Ryang Kim, Tatsushi Yokoyama, Sayaka Takemoto-Kimura, Manabu Abe, Michiko Okamura, Yayoi Kondo, Sean Quirin, Charu Ramakrishnan, Takeshi Imamura, Kenji Sakimura, Tomomi Nemoto, Masanobu Kano, Hajime Fujii, Karl Deisseroth, Kazuo Kitamura, Haruhiko Bito
Article
Multidisciplinary Sciences
James H. Marshel, Yoon Seok Kim, Timothy A. Machado, Sean Quirin, Brandon Benson, Jonathan Kadmon, Cephra Raja, Adelaida Chibukhchyan, Charu Ramakrishnan, Masatoshi Inoue, Janelle C. Shane, Douglas J. McKnight, Susumu Yoshizawa, Hideaki E. Kato, Surya Ganguli, Karl Deisseroth
Article
Biochemical Research Methods
Tong Zhang, Oscar Hernandez, Radoslaw Chrapkiewicz, Adam Shai, Mark J. Wagner, Yanping Zhang, Cheng-Hsun Wu, Jin Zhong Li, Masatoshi Inoue, Yiyang Gong, Biafra Ahanonu, Hongkui Zeng, Haruhiko Bito, Mark J. Schnitzer
Article
Neurosciences
Lief E. Fenno, Charu Ramakrishnan, Yoon Seok Kim, Kathryn E. Evans, Maisie Lo, Sam Vesuna, Masatoshi Inoue, Kathy Y. M. Cheung, Elle Yuen, Nandini Pichamoorthy, Alice S. O. Hong, Karl Deisseroth
Article
Biochemistry & Molecular Biology
Koichiro E. Kishi, Yoon Seok Kim, Masahiro Fukuda, Masatoshi Inoue, Tsukasa Kusakizako, Peter Y. Wang, Charu Ramakrishnan, Eamon F. X. Byrne, Elina Thadhani, Joseph M. Paggi, Toshiki E. Matsui, Keitaro Yamashita, Takashi Nagata, Masae Konno, Sean Quirin, Maisie Lo, Tyler Benster, Tomoko Uemura, Kehong Liu, Mikihiro Shibata, Norimichi Nomura, So Iwata, Osamu Nureki, Ron O. Dror, Keiichi Inoue, Karl Deisseroth, Hideaki E. Kato
Summary: ChRmine, a recently discovered pump-like cation-conducting channelrhodopsin, has opened new possibilities in optogenetics with its unique properties. The cryo-EM structure of ChRmine, revealed at 2.0A resolution, uncovers architectural features distinct from other channelrhodopsins and lays the foundation for the creation of new channelrhodopsins for various biological applications.
Article
Multidisciplinary Sciences
Brian Hsueh, Ritchie Chen, YoungJu Jo, Daniel Tang, Misha Raffiee, Yoon Seok Kim, Masatoshi Inoue, Sawyer Randles, Charu Ramakrishnan, Sneha Patel, Doo Kyung Kim, Tony X. Liu, Soo Hyun Kim, Longzhi Tan, Leili Mortazavi, Arjay Cordero, Jenny Shi, Mingming Zhao, Theodore T. Ho, Ailey Crow, Ai-Chi Wang Yoo, Cephra Raja, Kathryn Evans, Daniel Bernstein, Michael Zeineh, Maged Goubran, Karl Deisseroth
Summary: Emotional states influence bodily physiology, and it is unclear whether an increased heart rate can induce anxiety responses. Using noninvasive optogenetic methods, researchers found that optically induced tachycardia enhances anxiety-like behavior, suggesting that both central and peripheral processes are involved in the development of emotional states.
Article
Biochemistry & Molecular Biology
Seiya Tajima, Yoon Seok Kim, Masahiro Fukuda, Youngju Jo, Peter Y. Wang, Joseph M. Paggi, Masatoshi Inoue, Eamon F. X. Byrne, Koichiro E. Kishi, Seiwa Nakamura, Charu Ramakrishnan, Shunki Takaramoto, Takashi Nagata, Masae Konno, Masahiro Sugiura, Kota Katayama, Toshiki E. Matsui, Keitaro Yamashita, Suhyang Kim, Hisako Ikeda, Jaeah Kim, Hideki Kandori, Ron O. Dror, Keiichi Inoue, Karl Deisseroth, Hideaki E. Kato
Summary: KCR channelrhodopsins exhibit a distinctive mechanism for K+ selectivity, involving the formation of an asymmetric selectivity gate and a distinct dehydration pathway. The discovery of this mechanism provides a framework for studying ion-channel selectivity and advancing optogenetic applications.
Article
Biochemical Research Methods
Masayuki Sakamoto, Masatoshi Inoue, Atsuya Takeuchi, Shigetaka Kobari, Tatsushi Yokoyama, Shin-ichiro Horigane, Sayaka Takemoto-Kimura, Manabu Abe, Kenji Sakimura, Masanobu Kano, Kazuo Kitamura, Hajime Fujii, Haruhiko Bito
Summary: This study generates a transgenic mouse line that expresses a highly sensitive green GECI in specific neuronal populations. In vivo imaging reveals calcium transients in both excitatory and inhibitory neurons, providing a tool for investigating neuronal activity with enhanced resolution.
CELL REPORTS METHODS
(2022)
