Article
Biochemistry & Molecular Biology
Huiying Meng, Yunjuan Chen, Tingting Li, Huazhong Shi, Shuojun Yu, Yang Gao, Zhiqiang Wang, Xu Wang, Jian-Kang Zhu, Yechun Hong, Zhen Wang
Summary: The transcription factor AP2 plays a crucial role in ABA-mediated inhibition of seed germination through its interaction with ABI5. AP2 also interacts with SnRK2.2, SnRK2.3, and SnRK2.6, suggesting its multifaceted role in the ABA signaling pathway. These findings highlight the importance of the interactions of AP2 with SnRK2s and ABI5 in ABA signaling control of seed germination.
PLANT MOLECULAR BIOLOGY
(2023)
Article
Plant Sciences
Qi Zhang, Wei Zhang, Jianbo Wei, Zhaoxu Gao, Jianing Guan, Zhibo Cui, Xiaoxue Wang
Summary: This study reveals that the splicing factor SKIP plays a positive regulatory role in ABA signaling in plants. It regulates the splicing of ABA signaling-related genes, affecting the expression of ABA-positive regulators. The study also found that SKIP is involved in the regulation of ABA-mediated genome-wide alternative splicing and differential gene expression.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Plant Sciences
Jianwen Song, Lele Shang, Xin Wang, Yali Xing, Wei Xu, Yuyang Zhang, Taotao Wang, Hanxia Li, Junhong Zhang, Zhibiao Ye
Summary: This study identified a novel gene, MAPK11, which plays a critical role in seed germination of tomato by regulating ABA signaling and SnRKs phosphorylation. Understanding this mechanism provides a new perspective on seed germination and has implications for agricultural production efficiency.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Jiameng Chen, Zeyan Jin, Longyi Xiang, Yanyan Chen, Jie Zhang, Jiayi Zhao, Fudeng Huang, Yongfeng Shi, Fangmin Cheng, Gang Pan
Summary: This study investigated the impact of ethanol on reactive oxygen species (ROS), superoxide dismutase (SOD) activities, and hormone balance during rice seed germination. The results showed that ethanol inhibited seed germination by suppressing SOD activities, resulting in reduced H2O2 levels in rice germinating seed embryos. Ethanol also regulated ABA and GA levels in embryos, leading to disharmonizing GA/ABA balance. Additionally, ethanol promoted ABA signaling by upregulating ABA receptor genes and ABA-responsive element (ABRE)-binding protein/ABRE-binding factors.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Guoming Wang, Kaijie Qi, Xin Gao, Lei Guo, Peng Cao, Qionghou Li, Xin Qiao, Chao Gu, Shaoling Zhang
Summary: In this study, the evolution, characteristics, and expression patterns of PYL genes in Rosaceae plants were investigated. Whole-genome duplication and dispersed duplication were found to be the main drivers for the expansion of PYL gene family, and purifying selection was the major force in PYL gene evolution. qRT-PCR validation revealed the important roles of PYL genes in seed germination and abiotic stress responses in Chinese white pear.
Review
Plant Sciences
Ewa Sybilska, Agata Daszkowska-Golec
Summary: Seed germination is a vital process in a plant's life that is regulated by various mechanisms and external factors. Alternative splicing (AS) plays a significant role in abscisic acid (ABA) signaling and gene expression control. This review discusses the current understanding of AS regulators and ABA-related changes in AS during seed germination, as well as the impact of these changes on protein functionality. The advances in sequencing technology provide better tools for studying the role of AS in gene regulation.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Haijun Zhang, Yanhong Qiu, Yanhai Ji, Xuan Wu, Xiulan Xu, Ping Wu
Summary: This study demonstrates that melatonin promotes seed germination under low temperature stress by decreasing ABA content and increasing the GA/ABA ratio. The transcriptional regulation of ABA signaling genes by melatonin during cucumber seed germination under low temperature stress is significant.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Review
Biochemistry & Molecular Biology
Alberto Coego, Jose Julian, Jorge Lozano-Juste, Gaston A. Pizzio, Abdulwahed F. Alrefaei, Pedro L. Rodriguez
Summary: Post-translational modifications, particularly protein ubiquitylation, play a crucial role in regulating protein function and stability in various aspects of plant biology. Ubiquitin code not only targets substrates for proteolytic degradation but also regulates protein interactions, activity, and localization through nonproteolytic functions. This review focuses on the ubiquitylation of ABA receptors and PP2C coreceptors, as well as other post-translational modifications of ABA receptors that lead to their ubiquitination and degradation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yu-Rui Feng, Ting-Ting Li, Shi-Jia Wang, Ying-Tang Lu, Ting-Ting Yuan
Summary: Seed germination is a complex process regulated by various factors, with ABA playing a crucial role. TTM2 has been identified as an important factor in ABA-mediated seed germination. TTM2 expression is both enhanced and repressed by ABA, and it acts downstream of ABI4 in this process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Qidi Zhan, Jialu Shen, Kaili Nie, Yuan Zheng
Summary: In this study, the WD40 protein MIW1 was found to interact with MYB30, promoting its degradation and enhancing ABA-mediated inhibition of postgerminative development. MIW1 also promotes the expression of PYL genes by destabilizing MYB30, further enhancing ABA signaling.
Article
Plant Sciences
Lili Nian, Xiaoning Zhang, Xianfeng Yi, Xuelu Liu, Noor ul Ain, Yingbo Yang, Xiaodan Li, Fasih Ullah Haider, Xiaolin Zhu
Summary: This study identified and analyzed 39 MsPYL genes in alfalfa, revealing their distribution on 24 chromosomes and induction of expression by low-temperature treatment. The analysis provided insights into the structure and function of the alfalfa PYL gene family.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2021)
Article
Biotechnology & Applied Microbiology
Yuqing Rong, Tiezheng Li, Xiao Liu, Shepo Shi, Xiaohui Wang, Pengfei Tu
Summary: The study revealed that AsTal1 regulates ABA signaling-mediated seed germination and root growth by modulating the expression of genes involved in the ABA signaling pathway and enzymes responsive to ROS.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Plant Sciences
Lin Liang, Qi Wang, Zihao Song, Yaxin Wu, Qing Liang, Qingsong Wang, Jinli Yang, Ying Bi, Wen Zhou, Liu-Min Fan
Summary: The study reveals the interaction between SPY and CPN20, which is enhanced by ABA, and the O-fucosylation of CPN20 by SPY fine-tunes ABA signaling in Arabidopsis. SPY negatively regulates the localization of CPN20 in the chloroplast, and the role of CPN20 in the ABA signaling pathway may involve parallel pathways.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Zhaohan Zhang, Wanpeng Wang, Shahid Ali, Xiao Luo, Linan Xie
Summary: This study used the CRISPR/Cas9 gene editing system to knock out three PYL genes in soybeans, resulting in mutants with decreased sensitivity to ABA. The mutant plants also exhibited increased height and branch number compared to the wild type. GO enrichment analysis revealed activation of positive germination regulators under ABA stress, reducing ABA sensitivity and enhancing seed germination.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Yanrong Liu, Dedai Jiang, Jianping Yan, Kexin Wang, Shiwen Lin, Wanjun Zhang
Summary: The sensitivity of alfalfa seed germination to exogenous ABA treatment is linked to its drought tolerance. Screening alfalfa seedlings during germination with exogenous ABA can be used to obtain drought tolerant germplasm.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Environmental Sciences
Yue Zhu, Wenmin Qiu, Yuhong Li, Jinjuan Tan, Xiaojiao Han, Longhua Wu, Yugen Jiang, Zhiping Deng, Chao Wu, Renying Zhuo
Summary: Sedum plumbizincicola is a herbaceous species capable of accumulating excessive cadmium, especially with up-regulation of transport proteins, in response to Cd exposure. The study identified and quantified Cd-responsive membrane proteins in root, stem, and leaf tissues, showing significant changes in transport proteins such as ABC transporters and aquaporins after Cd treatment. The results provide insights into the molecular mechanisms of Cd hyper-tolerance and hyper-accumulation in S. plumbizincicola for potential applications in genetic engineering for phytoremediation purposes.
Article
Biochemistry & Molecular Biology
Hongtao Ji, Renhao Xiao, Xiangguang Lyu, Jiahuan Chen, Xuehai Zhang, Zhijuan Wang, Zhiping Deng, Yongliang Wang, Hui Wang, Ran Li, Qingqing Chai, Yongfang Hao, Qi Xu, Junwen Liao, Qian Wang, Yu Liu, Ruizhen Tang, Bin Liu, Xia Li
Summary: Darkness promotes root symbiotic nodulation, while blue light inhibits nodulation. Soybean TGACG motif-binding factors STF1 and STF2 regulate nodulation by repressing the transcription of the nodulation regulator GmNINa. Photoreceptor GmCRY1 promotes nodulation in darkness and mediates blue light-induced transcription of STF1 and STF2. The blue light receptor GmCRY1s-STF1/2 module plays a pivotal role in integrating darkness, blue light, and nodulation signals.
Article
Environmental Sciences
Yue Zhu, Wenmin Qiu, Xiaoyang He, Longhua Wu, De Bi, Zhiping Deng, Zhengquan He, Chao Wu, Renying Zhuo
Summary: Transcriptional and proteomic analyses were used to investigate the response of S. plumbizincicola plants to Cd stress, identifying several metabolic pathways potentially involved in Cd tolerance and accumulation. Staining and accumulation assays of transgenic lines over-expressing a Cd up-regulated gene provided insight into its role in lignin biosynthesis and Cd accumulation under Cd stress.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biochemistry & Molecular Biology
Zhi-Liang Yue, Ning Liu, Zhi-Ping Deng, Yu Zhang, Zhi-Ming Wu, Ji-Long Zhao, Ying Sun, Zhi-Yong Wang, Sheng-Wei Zhang
Summary: This study reveals the important role of the OsWAK11 gene in rice, which regulates agronomical traits and BR signaling by controlling cell elongation rates. OsWAK11 directly interacts with the BR receptor OsBRI1 and inhibits BR signaling. Additionally, OsWAK11 shows a stronger affinity for methyl-esterified pectin and is regulated by changes in pectin methylesterification in response to light.
Article
Biochemistry & Molecular Biology
Liqun Ma, Yongfang Yang, Yuqiu Wang, Ke Cheng, Xiwen Zhou, Jinyan Li, Jingyu Zhang, Ran Li, Lingling Zhang, Keru Wang, Ni Zeng, Yanyan Gong, Danmeng Zhu, Zhiping Deng, Guiqin Qu, Benzhong Zhu, Daqi Fu, Yunbo Luo, Hongliang Zhu
Summary: The glycine-rich RNA-binding protein SlRBP1 regulates the translation of key transcripts associated with chloroplast function, maintaining chloroplast functions. Knockdown mutants of SlRBP1 showed reduced chlorophyll accumulation and impaired chloroplast ultrastructure, accompanied by deregulation of key transcripts. SlRBP1 interacts with SleIF4A2 and promotes the translation of its target RNAs, thereby regulating chloroplast functions.
Article
Multidisciplinary Sciences
Xuanpeng Wang, Lili Wang, Yongchi Huang, Zhiping Deng, Cunliang Li, Jian Zhang, Mingxi Zheng, Shunping Yan
Summary: This study reveals the important role of a plant-specific ubiquitin E3 ligase, DDRM1, in homologous recombination repair (HR), which is essential for maintaining genome stability. DDRM1 interacts with and ubiquitinates the transcription factor SOG1, promoting its stability. This research uncovers the regulatory mechanism of HR in plants and highlights the significance of ubiquitination in HR.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Zhongjing Zhou, Baogang Lin, Jinjuan Tan, Pengfei Hao, Shuijin Hua, Zhiping Deng
Summary: In this study, a quantitative proteomic approach was used to compare differentially expressed proteins in high oil content and low oil content Brassica napus seeds. The results showed that these differentially expressed proteins were mainly involved in lipid biosynthesis and metabolism, photosynthesis, and nutrient reservoir activity. Western blot analysis confirmed that overexpression of a late embryogenesis abundant protein (BnLEA57) can enhance oil content. This study provides new insights into the molecular regulatory mechanism of seed oil content in B. napus.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jiayun Xing, Jinjuan Tan, Hanqian Feng, Zhongjing Zhou, Min Deng, Hongbing Luo, Zhiping Deng
Summary: This study investigates the molecular mechanism of cold adaptation in maize by analyzing the proteome and phosphoproteome levels of maize seedlings under cold shock. The results reveal the early molecular events associated with cold response in maize, including changes in photosynthesis proteins, spliceosome proteins, kinases, and transcriptional factors. The study provides valuable insights into how C-4 plants respond to sudden temperature drops.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Leia Colin, Felix Ruhnow, Jian-Kang Zhu, Chunzhao Zhao, Yang Zhao, Staffan Persson
Summary: Salt stress causes ionic toxicity, osmotic stress, and oxidative stress, directly affecting plant growth and development. Plants perceive salt stress through the cell wall and coordinate effective responses. Some mechanisms underlying these processes have been elucidated.
Article
Multidisciplinary Sciences
Henriette Rubsam, Christina Kronauer, Nikolaj B. Abel, Hongtao Ji, Damiano Lironi, Simon B. Hansen, Marcin Nadzieja, Marie Kolte, Dorte Abel, Noor de Jong, Lene H. Madsen, Huijun Liu, Jens Stougaard, Simona Radutoiu, Kasper R. Andersen
Summary: This study developed a synthetic approach using nanobodies to understand and activate multicomponent receptor complexes, successfully manipulating receptors involved in plant symbiosis with nitrogen-fixing bacteria. The authors found that the Lotus japonicus Nod factor receptors NFR1 and NFR5 form the core receptor complex for initiating root nodule organogenesis and controlling infection. They also discovered that organogenesis signaling is mediated by intracellular kinase domains, while infection requires functional ectodomains. Additionally, evolutionarily distant barley receptors were identified, which could potentially enable nitrogen-fixation engineering in cereals.
Article
Biochemistry & Molecular Biology
Jihong Zhang, Yuqiong Li, Shenglong Du, Zhiping Deng, Quanwu Liang, Ge Song, Haihua Wang, Mingli Yan, Xuewen Wang
Summary: In this study, the global mechanism underlying (E)-2-hexenal-mediated regulation of biotic stress tolerance in tomato was investigated using transcriptomic and proteomic analyses. The results showed that (E)-2-hexenal treatment reduced susceptibility to B. cinerea and increased antioxidant enzyme activities. The analysis also revealed the modulation of genes involved in various metabolic pathways and the activities of defense response proteins.
PLANT MOLECULAR BIOLOGY
(2023)
Article
Biochemical Research Methods
Bo Yu, Wenna Zheng, Staffan Persson, Yang Zhao
Summary: In this paper, a protocol for studying plant halotropism is presented, using an optimized split-agar system that simulates the salt gradient in soil. The steps for preparation of the split-agar system, measurement of Na+, and observation of root bending are described, along with the segmentation of root cells and visualization of microtubules and cellulose synthases. This system is simple to operate and has broader applications, such as hydrotropism and chemotropism.
Review
Plant Sciences
Hanqian Feng, Jinjuan Tan, Zhiping Deng
Summary: Ubiquitination and deubiquitination are crucial post-translational modifications in plants. UBP12 and UBP13, two deubiquitinating enzymes, play significant roles in regulating hormone signaling, developmental signaling, and transcription factor activity in plants.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Zhongjing Zhou, Zhiping Deng, Shuang Liang, Xiaowei Zou, Yi Teng, Weike Wang, Lizhong Fu
Summary: A rapid, precise, and dependable method for quantifying flavonoids in the fruiting bodies of Sanghuangporus was established using ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QQQ-MS/MS). The method enabled simultaneous detection of 10 flavonoids and can be applied to analyze flavonoid content in different Sanghuangporus species. This protocol serves as a valuable tool for identifying species with high levels of specific flavonoid compounds.
Article
Plant Sciences
Chuanling Li, Xuetong Li, Zhiping Deng, Yuning Song, Xinye Liu, Xiaohan Alex Tang, Ziye Li, Ya Zhang, Baowen Zhang, Wenqiang Tang, Jian-Xiu Shang, Yu Sun