期刊
MOLECULAR CELL
卷 79, 期 2, 页码 207-+出版社
CELL PRESS
DOI: 10.1016/j.molcel.2020.05.030
关键词
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资金
- Howard Hughes Medical Institute
- Gordon Ross Medical Foundation
- Benjamin M. Rosen graduate fellowship
- Biological Imaging Center at the Caltech Beckman Institute
- Millard and Muriel Jacobs Genetics and Genomics Laboratory
RNA polymerase II (RNA Pol II) contains a disordered C-terminal domain (CTD) whose length enigmatically correlates with genome size. The CTD is crucial to eukaryotic transcription, yet the functional and evolutionary relevance of this variation remains unclear, Here, we investigate how CTD length and disorder influence transcription. We find that length modulates the size and frequency of transcriptional bursting. Disorder is highly conserved and facilitates CTD-CTD interactions, an ability we show is separable from protein sequence and necessary for efficient transcription. We build a data-driven quantitative model, simulations of which recapitulate experiments and support that CTD length promotes initial polymerase recruitment to the promoter and slows down its release from it and that CTD-CTD interactions enable recruitment of multiple polymerases. Our results reveal how these parameters provide access to a range of transcriptional activity, offering a new perspective for the mechanistic significance of CTD length and disorder in transcription across eukaryotes.
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