期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 510, 期 -, 页码 -出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2020.110841
关键词
Glyphosate; Epithelial-mesenchymal- transition; Endometrial cancer; Estrogen receptor pathway
资金
- Agencia Nacional de Promocion Cientifica y Tecnologica (ANPCyT)
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)
- Binational PhD Program of Technische Universitat Dresden (TUD, Dresden, Germany)
- Facultad de Bioquimica y Ciencias Biologicas, Universidad Nacional del Litoral (FBCB, UNL, Santa Fe, Argentina)
- German Academic Exchange Service (DAAD: Deutscher Akademischer Austauschdienst)
Glyphosate based herbicides are the most commonly used herbicide in the world. We aimed to determine whether glyphosate (Gly) induces epithelial mesenchymal transition (EMT) - related changes in a human endometrial carcinoma cell line (Ishikawa cells), and whether the estrogen receptor (ER) pathway is involved in these changes. Ishikawa cells were exposed to Gly (0.2 mu M and 2 mu M) or 17 beta-estradiol (E2: 10(-9) M). We detected that Gly increased cell migration and invasion ability compared to vehicle, as did E2. Moreover, a down regulation of E-cadherin mRNA expression was determined in response to Gly, similar to E2-effects. These results show that Gly promotes EMT-related changes in Ishikawa cells. When an ER antagonist (Fulvestrant: 10(-7) M) was co-administrated with Gly, all changes were reversed, suggesting that Gly might promote EMT-related changes via ER-dependent pathway. Our results are interesting evidences of Gly effects on endometrial cancer progression via the ER-dependent pathway.
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