期刊
MICROCHIMICA ACTA
卷 187, 期 7, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-020-04330-7
关键词
Au@DTNB@Ag core-shell nanoparticles; Surface-enhanced Raman scattering; miRNA-21; Duplex-specific nuclease; Human serum
资金
- National Natural Science Foundation of China [61875255]
- Hefei Center of Physical Science Technology [2018ZYFX005]
A novel surface-enhanced Raman scattering (SERS) analysis strategy has been designed combining Au@DTNB@Ag core-shell nanoparticles (DTNB attachment on gold nanoparticles, then encapsulated in Ag shell nanoparticles named as ADANPs) and duplex-specific nuclease signal amplification (DSNSA) platform. Firstly, ADANPs and magnetic substrate of Fe3O4 nanoparticles were covalently attached to the 3 '- and 5 '- end of capture probe (CP) targeting miRNA-21. Upon the addition of target miRNA-21, these heteroduplexes were specifically cleaved by DSN and resulted in ADANPs that were released from the surface of Fe3O4 nanoparticles (Fe3O4 NPs). At the same time, miRNA-21 remained intact and can rehybridize another DNA probe to trigger the signal-amplifying reaction. Based on this principle, the developed SERS method exhibited good linearity in the range 0 to 1 nM for miRNA-21 with a limit of detection (LOD) of 0.084 fM and has an ability to differentiate even a single-base mismatched sequence on the target sequence or other miRNA sequence. The results provide a novel SERS method which can successfully been applied to the miRNA-21 detection in human serum.
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