Article
Chemistry, Analytical
Xinwen Sun, Xiaoru Dai, Shisheng Ling, Wenkun Dong, Dong Chen, Mengting Li, Xvsheng Qiao, Zhiyu Wang, Xianping Fan, Guodong Qian
Summary: A lateral flow immunochromatographic strip (LFIS) based on Yb/Er co-doped NaYF4 upconversion nanoparticles (UCNPs) was developed for rapid and sensitive detection of myocardial infarction. The strip showed high sensitivity and specificity for detecting serum amyloid A (SAA) in only 10 mu L of serum. This UC-LFIS holds great potential for the early diagnosis and prognosis of cardiovascular diseases (CVDs).
Article
Chemistry, Analytical
Wanghong He, Minli You, Zedong Li, Lei Cao, Feng Xu, Fei Li, Ang Li
Summary: The study developed a lateral flow immunoassay strip platform for simultaneous detection of three periodontitis biomarkers using upconversion nanoparticles, achieving successful results in clinical samples. The platform demonstrated high sensitivity and detection specificity, as well as rapid detection capabilities.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Zhaorui Song, Yongkuan Suo, Shuang Duan, Shanshan Zhang, Lifu Liu, Botong Chen, Zhen Cheng
Summary: Fluorescent lateral flow immunoassay (LFA) based on rare earth doped nanoparticles (RENPs) emitting in the second near-infrared (NIR-II) window is a common analytical platform for point-of-care testing (POCT) that enables easy and early screening of biomarkers. This platform shows high sensitivity, low cost, and has potential for early diagnosis of tumor biomarkers.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Wei Wang, Zihan Ye, Xing Ma, Jinhong Guo
Summary: The combination of upconversion nanoparticles and lateral flow assays, along with computer vision technology, enables the instantaneous quantitative detection of small molecule targets. The device provides accurate quantitative data for small molecule targets in a fast and portable manner.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Environmental Sciences
Yanfeng Sun, Yanjian Wan, Ying Jiang, Huaiji Wang
Summary: The study found that exposure to Acetaminophen (AAP) was common in young children in central and south China, with AAP concentrations positively correlated with oxidative stress biomarkers 8-hydroxy-guanosine (8-OHG) and 8-hydroxy-2'-deoxyguanosine (8-OHdG). This suggests a potential association between AAP exposure and DNA and RNA damage.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Analytical
Kyeongnyeon Kim, Do Kyoung Han, Namhyun Choi, Soo Hyeon Kim, Younju Joung, Kihyun Kim, Ngoc Thanh Ho, Sang-Woo Joo, Jaebum Choo
Summary: This study developed a new concept of using a dual-flow SERS-based LFA sensor to improve detection sensitivity by flowing two different sizes of gold nanoparticle solutions. The sensor successfully detected thyroid-stimulating hormone less than 0.5 mu IU/mL, which cannot be measured with a SERS-based single-flow LFA sensor.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Xuejiao Hu, Jianfeng Liao, Huizhuang Shan, Hao He, Zhongbo Du, Ming Guan, Jiwen Hu, Jing Li, Bing Gu
Summary: We developed a novel upconversion nanoparticle-based fluorescence lateral flow test strip for the qualitative and quantitative detection of prostate-specific antigen (PSA). The strip demonstrated excellent accuracy, anti-interference, and stability in the gray zone (4-10 ng/mL) of PSA clinical application and outperformed other PSA test strips.
Article
Chemistry, Analytical
Liangke Gong, Kuiyu Wang, Jianwei Liang, Liren Zhang, Tao Yang, Hui Zeng
Summary: Food borne pathogens pose a threat to food safety and human health. Lateral flow immunoassays (LFIAs) have gained attention for their simplicity, low cost, and user friendliness, and show potential in pathogen detection. However, the sensitivity of LFIAs is limited. In this study, multi-line LFIAs were introduced for the first time in pathogen detection, resulting in significantly improved overall signal strength and increased detection accuracy. Real samples of milk and orange juice were used for validation, demonstrating the potential of this strategy for rapid pathogen detection and improving the accuracy and sensitivity of LFIAs strips.
Article
Chemistry, Analytical
Qiang Fu, Lin Yuan, Fengrong Cao, Lihua Zang, Dandan Ji
Summary: The study developed a rapid, simple, and quantitative detection method for Salmonella using a novel biosensor, which can complete the testing in less than 40 minutes without the need for large laboratory equipment or analytical instruments.
MICROCHEMICAL JOURNAL
(2021)
Review
Chemistry, Analytical
Donato Calabria, Maria Maddalena Calabretta, Martina Zangheri, Elisa Marchegiani, Ilaria Trozzi, Massimo Guardigli, Elisa Michelini, Fabio Di Nardo, Laura Anfossi, Claudio Baggiani, Mara Mirasoli
Summary: Paper-based lateral-flow immunoassays have achieved commercial success and are continuously growing in the field of diagnostics. These assays, which provide qualitative information about the target analyte, have the potential to be adapted for ultrasensitive quantitative analysis.
Article
Chemistry, Analytical
Yewon Kwon, Dami Kim, Sanghyo Kim
Summary: In this study, a quantitative injection (QI) strip was designed by modifying the structure of lateral flow immunoassay (LFA) strips to achieve the injection of the same unit volume. Compared to the LFA strip, the QI strip maintained consistent detection signals even with increasing volume of injected sample and demonstrated a lower deviation. Therefore, the QI strip is more suitable for quantitative analysis without the need for additional equipment such as a pipette.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Raluca-Ioana Stefan-van Staden, Oana-Raluca Musat
Summary: This article proposes two stochastic microsensors based on single-walled and multi-walled carbon nanotubes modified with alpha-cyclodextrin for the assay of 8-hydroxy-2'-deoxyguanosine in whole blood. The microsensor based on single-walled carbon nanotubes showed a wider linear concentration range, while the microsensor based on multi-walled carbon nanotubes showed the highest sensitivity.
MICROCHEMICAL JOURNAL
(2023)
Review
Chemistry, Multidisciplinary
Amadeo Sena-Torralba, Ruslan Alvarez-Diduk, Claudio Parolo, Andrew Piper, Arben Merkoci
Summary: This article reviews the application of nanomaterial-based lateral flow assays (LFAs) in point-of-care diagnostics. By incorporating novel nanomaterials as signal transducers or receptor immobilization platforms, the analytical capabilities of LFAs have been improved. The article highlights strategies that can address the limitations of current LFAs and have shown commercial feasibility.
Article
Chemistry, Analytical
Natpapas Wiriyachaiporn, Siriwan Sirikaew, Suwussa Bamrungsap, Thanchanok Limcharoen, Pannarai Polkankosit, Pimporn Roeksrungruang, Kanyawan Ponlamuangdee
Summary: A simple fluorescence-based lateral flow test platform was developed for rapid screening of influenza B virus. The system utilizes Cy5-loaded silica nanoparticles directly conjugated to monoclonal antibodies for detection, showing good sensitivity and specificity under optimized conditions. Detection can be performed within 30 minutes with small sample volumes, and the system demonstrates no cross-reactivity to other closely related respiratory viruses.
ANALYTICAL METHODS
(2021)
Article
Multidisciplinary Sciences
Yue Zhang, Peng Cao, Fang Lu, Jinjun Cheng, Huihua Qu
Summary: The protocol provides a detailed description on developing a rapid, sensitive and quantitative lateral flow immunoassay, which can be used for low-cost self-diagnosis and provide an alternative tool for rapid and precise analysis of physiological and biological indices.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)