Article
Biochemistry & Molecular Biology
Inga R. Grin, Grigory Mechetin, Rustem D. Kasymov, Evgeniia A. Diatlova, Anna Yudkina, Sergei N. Shchelkunov, Irina P. Gileva, Alexandra A. Denisova, Grigoriy A. Stepanov, Ghermes G. Chilov, Dmitry O. Zharkov
Summary: Uracil-DNA glycosylases are crucial enzymes for the efficiency of antimetabolite drugs and virulence in certain infections. Existing UNG inhibitors are based on uracil base, but new pharmacophores like PyO3 are needed for a wider range of UNGs. PyO3 shows potential as a novel pharmacophore for UNG-targeting agents.
Article
Multidisciplinary Sciences
Woong Sik Jang, Da Hye Lim, Jung Yoon, Ahran Kim, Minsup Lim, Jeonghun Nam, Richard Yanagihara, Sook-Won Ryu, Bo Kyeung Jung, Nam-Hee Ryoo, Chae Seung Lim
Summary: In this study, three RT-LAMP primer sets were designed for detecting different genes of SARS CoV-2. It was found that the detection limits of two primer sets were low, showing comparable sensitivity to other widely used detection methods.
Article
Biotechnology & Applied Microbiology
Likui Zhang, Donghao Jiang, Qi Gan, Haoqiang Shi, Li Miao, Yong Gong, Philippe Oger
Summary: Tba UDG247 is a novel bifunctional glycosylase that can excise uracil from DNA and further cleave the phosphodiester bond of the generated AP site. Kinetic analyses show that the enzyme has a faster rate for uracil excision than for AP cleavage, suggesting that cleaving AP site is a rate-limiting step for its bifunctionality.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Yee Ling Lau, Ilyiana Binti Ismail, Nur Izati Binti Mustapa, Meng Yee Lai, Tuan Suhaila Tuan Soh, Afifah Haji Hassan, Kalaiarasu M. Peariasamy, Yee Leng Lee, Maria Kahar Bador Abdul Kahar, Jennifer Chong, Pik Pin Goh
Summary: RT-RPA is a rapid and accurate detection method for SARS-CoV-2, with a clinical sensitivity of 98% and specificity of 100%, proving to be a viable alternative to RT-qPCR.
Article
Biochemistry & Molecular Biology
Paul B. Orndorff, Arjan van der Vaart
Summary: Uracil damaged DNA largely follows trends in flexibility of undamaged DNA. The flexibility of DNA is increased by uracil, and U:G base paired strands are more flexible than U:A strands. Certain sequence contexts, especially the 3' base next to uracil, play a key role in DNA deformability.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Virology
Wael Muselmani, Naail Kashif-Khan, Claire Bagneris, Rosalia Santangelo, Mark A. Williams, Renos Savva
Summary: Proteins encoded by viruses that mimic DNA can regulate processes like innate cellular immunity. An example is the inhibition of Ung-family uracil-DNA glycosylase, which blocks Ung-mediated degradation by binding to the DNA-binding site. This is important for the replication and distribution of virus genomes where uracil-DNA plays a crucial role.
Article
Chemistry, Analytical
Guohua Qi, Chen Xu, Jiafeng Wang, Yu Tian, Bo Wang, Ying Zhang, Kongshuo Ma, Xingkang Diao, Yongdong Jin
Summary: This study presents a new technique for promoting cell differentiation through optoplasmonic method, which can significantly shorten cell differentiation time, enhance cellular bioenergetics levels, and potentially be applied in tissue repair and regeneration.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Xiaolei Song, Qin Ding, Juan Zhang, Rongli Sun, Lihong Yin, Wei Wei, Yuepu Pu, Songqin Liu
Summary: A smart catalyzed hairpin assembly (CHA) DNAzyme nanosystem has been developed for intracellular UDG imaging, utilizing MnO2 nanosheets as nanocarriers to efficiently deliver DNA probes into cells and promote the fluorescence resonance energy transfer response. This approach offers enhanced sensitivity for early-stage cancer prediction and diagnosis.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Mai Wu, Tan Lin, Kunming Dong, Yong Gong, Xipeng Liu, Likui Zhang
Summary: This study characterizes a family IV uracil DNA glycosylase (Sis-UDGIV) from the hyperthermophilic crenarchaeon Sulfolobus islandicus REY15A and investigates the roles of nine conserved residues in uracil excision and four conserved cysteine residues in maintaining protein conformation and stability. The results provide insights into the biochemical function and DNA-binding specificity of archaeal family IV UDGs.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Analytical
Tong Zhang, Wei Zhao, Wang Zhao, Yuying Si, Nianzhen Chen, Xi Chen, Xinlian Zhang, Lieying Fan, Guodong Sui
Summary: A new CRISPR-LAMP technology has been developed for rapid and accurate diagnosis of respiratory tract viruses, with increased sensitivity and reduced aerosol contamination. The technology combines different techniques and designs to precisely diagnose pathogens and efficiently detect point mutations.
ANALYTICAL CHEMISTRY
(2021)
Article
Plant Sciences
Mieun Lee, Yu Been Heo, Han Min Woo
Summary: Base editing enables precise gene editing without donor DNA or double-stranded breaks. However, the uracil DNA glycosylase inhibitor (UGI) fused with the cytidine deaminase-Cas nickase cannot inhibit the archaic Type IV uracil DNA glycosylase in cyanobacteria. To overcome this limitation, dCas12a-assisted gene repression of the udg gene allowed base editing with high mutation frequencies in cyanobacteria.
Article
Chemistry, Analytical
Guozhen Tian, Wenting Li, Biao Liu, Meifang Xiao, Qianfeng Xia
Summary: We propose a novel electrochemical biosensing platform-based strategy for detecting abnormal expressions of uracil-DNA glycosylase (UDG). This method combines NiC-oP@PtCu nanozyme with multi-MNAzyme junctions to achieve ultrasensitive UDG detection. The biosensor is enzyme-free, exhibits a large dynamic range, and has potential applications in clinical diagnosis and biomedical research.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemistry & Molecular Biology
Jing Li, Ye Yang, Chenyan Chang, Weiguo Cao
Summary: Uracil-DNA glycosylase (UDG) is an enzyme involved in base excision repair and mammalian demethylation. This study identifies a novel UDG enzyme that requires acidic conditions for its glycosylase activity. The study investigates the catalytic motifs and key residues of the enzyme and reveals its role in mutation prevention and stress resistance.
Article
Microbiology
Helena Fabryova, Sandra Kao, Sayaka Sukegawa, Eri Miyagi, Louis Taylor, Damien Ferhadian, Hideki Saito, Heiner Schaal, Frank Hillebrand, Klaus Strebel
Summary: Despite progress in drug therapies, HIV-1 remains incurable and can rebound due to drug resistance. Gelsolin (GSN) was identified as a novel Vpr-interacting protein and its expression was associated with HIV-1 infection in macrophages. Overexpression of GSN reduces the expression and packaging of HIV-1 envelope protein, leading to reduced viral infectivity.
Article
Biochemistry & Molecular Biology
Ryan J. J. Buckley, Anna Lou-Hing, Karl M. M. Hanson, Nadia R. R. Ahmed, Christopher D. O. Cooper, Edward L. L. Bolt
Summary: DNA glycosylases play a crucial role in maintaining genetic fidelity during DNA replication by eliminating chemically damaged DNA bases that could lead to mutations. In this study, researchers identified Lhr protein in Escherichia coli as a uracil-DNA glycosylase that is independent of its DNA helicase activity. The deletion of the lhr gene in E. coli sensitized cells to oxidative stress and increased cytosine deamination to uracil. This suggests that Lhr protein functions in surveillance and removal of potentially mutagenic base changes during replication stress.
MOLECULAR MICROBIOLOGY
(2023)
Article
Oncology
Stephanie Durand-Panteix, Jacques Monteil, Magali Sage, Armand Garot, Marie Clavel, Amal Saidi, Julien Torgue, Michel Cogne, Isabelle Quelven
Summary: This study investigated the potential of alpha-RIT in combination with Pb-212-rituximab for NHL treatment using mouse lymphoma cells. Results showed significant tumor regression and increased survival using 277.5 kBq Pb-212-rituximab, highlighting the potency of alpha-RIT for NHL treatment.
BRITISH JOURNAL OF CANCER
(2021)
Article
Allergy
Anne Marchalot, Catherine Horiot, Jean-Marie Lambert, Claire Carrion, Christelle Oblet, Justine Pollet, Michel Cogne, Jeanne Moreau, Brice Laffleur, Laurent Delpy
Summary: This study demonstrates the effectiveness of an antisense oligonucleotide (ASO) strategy in reducing IgE secretion and promoting allergen tolerance by inducing apoptosis of IgE1 antibody-secreting cells.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Urology & Nephrology
Vincent Javaugue, Virginie Pascal, Sebastien Bender, Sarah Nasraddine, Mathilde Dargelos, Mehdi Alizadeh, Alexis Saintamand, Matthieu Filloux, Paco Derouault, Sabrina Bouyer, Estelle Desport, Arnaud Jaccard, Frank Bridoux, Michel Cogne, Christophe Sirac
Summary: The study developed a high throughput sequencing assay to detect subtle clonal disorders in monoclonal gammopathy of renal significance, which could potentially improve the management of this kidney disease.
KIDNEY INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Melanie Rogier, Jacques Moritz, Isabelle Robert, Chloe Lescale, Vincent Heyer, Arthur Abello, Ophelie Martin, Katia Capitani, Morgane Thomas, Anne-Sophie Thomas-Claudepierre, Brice Laffleur, Florence Jouan, Eric Pinaud, Karin Tarte, Michel Cogne, Silvestro G. Conticello, Evi Soutoglou, Ludovic Deriano, Bernardo Reina-San-Martin
Summary: The study revealed that the interaction between FAM72A and UNG2 regulates class-switch recombination and somatic hypermutation in B cells. This finding suggests a new mechanism for controlling DNA repair processes and immune responses, providing insights into the balance between error-prone and error-free repair pathways.
Article
Multidisciplinary Sciences
Florian Reizine, Murielle Gregoire, Mathieu Lesouhaitier, Valentin Coirier, Juliette Gauthier, Celine Delaloy, Elise Dessauge, Florent Creusat, Fabrice Uhel, Arnaud Gacouin, Frederic Dessauge, Cecile Le Naoures, Caroline Moreau, Claude Bendavid, Yoann Daniel, Kilian Petitjean, Valerie Bordeau, Claire Lamaison, Caroline Piau, Vincent Cattoir, Mikael Roussel, Bernard Fromenty, Christian Michelet, Yves Le Tulzo, Jaroslaw Zmijewski, Ronan Thibault, Michel Cogne, Karin Tarte, Jean-Marc Tadie
Summary: Severe sepsis can lead to sustained immune dysfunction, resulting in organ failure, secondary infections, and late mortality. Supplementation with arginine or citrulline can restore T cell function and reduce immunosuppression induced by sepsis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Allergy
M. Cogne, Z. Dalloul, O. Deze, M. Le Goff, S. Thomas, M. Cahen, N. Ueda, B. Laffleur
Summary: Persisting adaptive humoral immunity relies on memory B lymphocytes and long-lived plasma cells. IgE responses, although protective against parasites and certain venoms, can have detrimental effects and result in chronic conditions with dramatic acute adverse events.
REVUE FRANCAISE D ALLERGOLOGIE
(2022)
Letter
Biochemistry & Molecular Biology
Diana Molino, Christine Durier, Anne Radenne, Corinne Desaint, Jacques Ropers, Soizic Courcier, Louis Victorien Vieillard, Claire Rekacewicz, Beatrice Parfait, Victor Appay, Frederic Batteux, Emmanuel Barillot, Michel Cogne, Behazine Combadiere, Christiane S. Eberhardt, Guy Gorochov, Philippe Hupe, Laetitia Ninove, Stephane Paul, Isabelle Pellegrin, Sylvie van der Werf, Maeva Lefebvre, Elisabeth Botelho-Nevers, Inmaculada Ortega-Perez, Marie Jaspard, Samba Sow, Jean Daniel Lelievre, Xavier de Lamballerie, Marie Paule Kieny, Eric Tartour, Odile Launay
Article
Immunology
Amelie Bos, Esil Aleyd, Lydia P. E. van der Steen, P. J. Winter, Niels Heemskerk, Stephan M. Pouw, Louis Boon, Rene J. P. Musters, Jantine E. Bakema, Cassian Sitaru, Michel Cogne, Marjolein van Egmond
Summary: IgA autoantibodies may play a role in autoimmune or chronic inflammatory diseases. A novel IgA autoimmune blistering model using genetically modified mice showed that IgA anti-collagen XVII could activate neutrophils and cause tissue damage, while treatment with anti-Fc alpha RI monoclonal antibodies prevented and resolved the disease.
FRONTIERS IN IMMUNOLOGY
(2022)
Editorial Material
Immunology
Michel Cogne, Thierry Fest, Said Aoufouchi
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Aymeric Neyret, Eric Bernard, Olivier Aiqui-Reboul-Paviet, William Bakhache, Patrick Eldin, Laurent Chaloin, Laurence Briant
Summary: Ras-GTPase-activating SH3 domain-binding proteins 1 and 2 (G3BP1 and G3BP2) are RNA-binding proteins that play multifunctional roles in stress granule nucleation and are essential cofactors for Old World alphaviruses. In this study, the researchers investigated the interaction between G3BP proteins and nsP3 protein of Mayaro virus (MAYV), an arthritogenic virus. They found that both G3BP1 and G3BP2 are required for MAYV growth in human cells and bind to the nsP3 protein. Surprisingly, deletion of the single FGxF sequence in MAYV nsP3 did not abolish the binding and viral infectivity. Instead, an upstream FGAP sequence was identified as an additional interaction motif. These findings reveal a non-conventional G3BP binding sequence in MAYV nsP3.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Oncology
Lina Zawil, Tiffany Marchiol, Baptiste Brauge, Alexis Saintamand, Claire Carrion, Elise Dessauge, Christelle Oblet, Sandrine Le Noir, Frederic Mourcin, Mylene Brousse, Paco Derouault, Mehdi Alizadeh, Yolla El Makhour, Celine Monvoisin, Julien Saint-Vanne, Simon Leonard, Stephanie Durand-Panteix, Karin Tarte, Michel Cogne
Summary: BCL2 is a commonly deregulated gene in various B-cell malignancies, and its deregulation may have different impacts on B-cell fate. This study designed two transgenic mouse models to compare the effects of BCL2 association with IgH3'RR and Ig kappa gene loci on B-cell fate and lymphoid tissues. The results showed that BCL2 association with IgH3'RR primarily impacted germinal center B-cell populations, while its association with Ig kappa locus primarily boosted the influx of plasma cells and the accumulation of long-lived plasma cells.
Review
Biochemistry & Molecular Biology
Ophelie Deze, Brice Laffleur, Michel Cogne
Summary: Mature B cells utilize class switch recombination (CSR) to diversify immunoglobulin (Ig) production, facilitated by activation-induced deaminase (AID) targeting cytosines in transcribed targeted S regions. G-rich sequences in S regions form canonical G-quadruplexes (G4s) DNA structures, favoring CSR. Small molecules interacting with G4-DNA (G4 ligands) can regulate CSR either positively or negatively. The specific regulatory role of G4 structures in CSR and lymphomagenesis remains a major issue in understanding immune responses.
Article
Immunology
Nicolas Denis-Lagache, Christelle Oblet, Tiffany Marchiol, Audrey Baylet, Ophelie Teteau, Iman Dalloul, Zeinab Dalloul, Lina Zawil, Ophelie Deze, Jeanne Cook-Moreau, Alexis Saintamand, Hend Boutouil, Ahmed Amine Khamlichi, Claire Carrion, Sophie Peron, Sandrine Le Noir, Brice Laffleur, Michel Cogne
Summary: This study investigates the deletion of a specific gene structure that is associated with antibody gene variation and class switching, using a new mouse model. The results suggest that this gene deletion is associated with an increase in self-reactive antibodies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Virginie Pascal, Marine Dupont, Paco de Rouault, David Rizzo, Delphine Rossille, Robin Jeannet, Thomas Daix, Bruno Francois, Steve Genebrier, Marie Cornic, Guillaume Monneret, Fabienne Venet, Juliette Ferrant, Mikael Roussel, Florian Reizine, Mathieu Le Souhaitier, Jean-Marc Tadie, Kartin Tarte, Jean Feuillard, Michel Cogne
Summary: To investigate the immune response architecture in COVID-19 patients and understand the factors that can cause or prevent ARDS, we analyzed the B cell responses using flow cytometry and Ig repertoire analysis. Flow cytometry with FlowSOM analysis revealed changes associated with COVID-19 inflammation, including an increase in double-negative B cells and ongoing plasma cell differentiation. We also found the expansion of two disconnected B cell repertoires. Analysis of Ig repertoire patterns showed an early expansion of IgG1 clonotypes with atypical CDR3 characteristics, which correlated with ARDS severity. Additionally, we observed a convergent response with progressively increasing somatic hypermutation and normal or short CDR3 in anti-SARS-CoV-2 clonotypes, which persisted until the memory B cell stage after recovery.
Article
Biochemistry & Molecular Biology
Ophelie Deze, Delfina Ordanoska, Delphine Rossille, Emma Miglierina, Brice Laffleur, Michel Cogne
Summary: Ig genes have the unique ability to be reshaped in B lymphocytes after encountering specific antigens, resulting in improved affinity and antibody production. Ig class switching, an important change in this process, is mediated by DNA repeats and DNA lesions. The discovery of IgH-like DNA repeats in the human IgH locus suggests their regulatory role in antibody production.