期刊
JOURNAL OF THE ROYAL SOCIETY INTERFACE
卷 17, 期 167, 页码 -出版社
ROYAL SOC
DOI: 10.1098/rsif.2020.0180
关键词
amperometry detection; cancer biomarkers; limit of detection; biosensor amplification
资金
- Griffith University
Nanozymes are nanomaterials with intrinsic magnetism and superparamagnetic properties. In the presence of an external magnet, nanozyme particles aggregate and redisperse without a foreign attraction. We evaluated the performances of nanozyme by changing the biosensing platforms and substituting other biological variants for a complete cancer assay detection. We investigated the expression of morphological variants in the transmission of signals using an electrochemical method. The signal responses, including signal enhancement with the nanozyme (Au-Fe2O3), showed a wide capturing range (greater than 80%, from 10(2) to 10(5) cells ml(-1) in phosphate-buffered saline buffer, pH 7.4). The platform showed a fast response time within a dynamic range of 10-10(5) cells ml(-1) for the investigated T47D cancer cell line. We also obtained higher responses for anti-HER2 (human epidermal receptor 2)/streptavidin interface as the biosensing electrode in the presence of T47D cancer cells. The positive assay produced a sixfold increase in current output compared to the negative target or negative biological variant. We calculated the limit of detection at 0.4 U ml(-1), and of quantitation at 4 U ml(-1) (units per millilitre). However, blood volume amounts in clinical settings may constrain diagnosis and increase detection limit value significantly.
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