期刊
CLINICAL IMMUNOLOGY
卷 173, 期 -, 页码 10-18出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.clim.2016.10.009
关键词
Food allergy; Allergen uptake; Intestinal barrier function; Cytokines; Microbiota; Bacterial community composition0
类别
资金
- Austrian Science Fund [WKP039, KLI284, P21884]
- FWF grant [DK W1205]
- FFG (Nano Health) [819721]
- Medical Scientific Fund of the Mayor of the City of Vienna [11013]
- Deutsche Forschungsgemeinschaft (Cluster of Excellence 'Inflammation at Interfaces') [EXC 306]
- German Federal Ministry of Education and Research (German Center for Lung Research, DZL)
- [SFB F4606-B28]
- [SFB F4615-B19]
- Austrian Science Fund (FWF) [KLI284, P21884, WKP39] Funding Source: Austrian Science Fund (FWF)
- Austrian Science Fund (FWF) [WKP 39, P 21884] Funding Source: researchfish
In our mouse model, gastric acid-suppression is associated with antigen-specific IgE and anaphylaxis development. We repeatedly observed non-responder animals protected from food allergy. Here, we aimed to analyse reasons for this protection. Ten out of 64 mice, subjected to oral ovalbumin (OVA) immunizations under gastric acid-suppression, were non-responders without OVA-specific IgE or IgG1 elevation, indicating protection from allergy. In these non-responders, allergen challenges confirmed reduced antigen uptake and lack of anaphylactic symptoms, while in allergic mice high levels of mouse mast-cell protease-1 and a body temperature reduction, indicative for anaphylaxis, were determined. Upon OVA stimulation, significantly lower IL-4, IL-5,1L-10 and IL-13 levels were detected in non-responders, while IL-22 was significantly higher. Comparison of fecal microbiota revealed differences of bacterial communities on single bacterial Operational-Taxonomic-Unit level between the groups, indicating protection from food allergy being associated with a distinct microbiota composition in a non-responding phenotype in this mouse model. (C) 2016 The Authors. Published by Elsevier Inc.
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