Article
Multidisciplinary Sciences
Anita Rogic, Ila Pant, Luca Grumolato, Ruben Fernandez-Rodriguez, Andrew Edwards, Suvendu Das, Aaron Sun, Shen Yao, Rui Qiao, Shabnam Jaffer, Ravi Sachidanandam, Guray Akturk, Rosa Karlic, Mihaela Skobe, Stuart A. Aaronson
Summary: Inflammatory breast cancer (IBC) is an aggressive form of breast cancer with a poor prognosis. The characterization of a human IBC cell line in this study suggests that the high level of CCL2 is implicated in macrophage infiltration and tumor progression.
NATURE COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Flavia S. Zandonadi, Sami Yokoo, Daniela Campos Granato, Cesar Rivera, Carolina Carneiro Soares Macedo, Ciro Dantas Soares, Carolina Moretto Carnielli, Romenia Ramos Domingues, Bianca A. Pauletti, Silvio Roberto Consonni, Ricardo D. Colleta, Adriana F. Paes Leme
Summary: This study investigates the role of Syndecan-1 (SDC1) and its interaction with FSTL1 in oral cancer. The results demonstrate that FSTL1 and SDC1 affect tumor-related events and that FSTL1 promotes a more aggressive tumor phenotype.
JOURNAL OF PROTEOMICS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Ke-Long Zou, Zhou Lan, Hao Cui, Yu-Yue Zhao, Wei-Ming Wang, Guang-Tao Yu
Summary: The high expression of CD24 in OSCC is associated with poor prognosis and is significantly correlated with CD68. Inhibiting CD24 can delay tumor growth, reduce TAMs number, increase T cell number, and impair tumor proliferation.
Article
Cell Biology
Yuehong Shen, Yuling Chen, Yuntao Lin, Yicun Li, Pengfei Liu, Biru Zhang, Yufan Wang, King-Chi Chan, Nai-Ki Mak, Michael Kahn, Robert Z. Z. Qi, Hongyu Yang
Summary: The role and action mechanism of CDK5RAP2 in oral squamous cell carcinoma (OSCC) have not been fully understood. It is highly expressed in OSCC and its expression correlates with clinical stage and lymph node metastasis. CDK5RAP2 expression is regulated by the Wnt signaling pathway. Depletion of CDK5RAP2 inhibits OSCC tumorigenesis and migration, and disrupts spindle orientation during mitosis, indicating its potential as a CSC marker.
CELL DEATH & DISEASE
(2023)
Article
Oncology
Sabrina Daniela da Silva, Fabio Albuquerque Marchi, Jie Su, Long Yang, Ludmila Valverde, Jessica Hier, Krikor Bijian, Michael Hier, Alex Mlynarek, Luiz Paulo Kowalski, Moulay A. Alaoui-Jamali
Summary: There is clinical evidence linking ulcerated and inflammatory cell-infiltrated oral cancer with early metastases. Genomic screening in patients with metastatic oral cancer revealed specific gene changes related to macrophage chemotaxis and tumor progression. Overexpression of TWIST1 and CSF1 was observed in metastatic cases, leading to poor patient survival, and further studies showed their cooperation in promoting oral squamous cell carcinoma invasiveness.
Article
Biochemistry & Molecular Biology
Simone Aparecida de Bessa Garcia, Mafalda Araujo, Tiago Pereira, Renata Freitas
Summary: HOX genes play a role in breast cancer progression depending on molecular subtypes, with HOXB7 overexpression potentially promoting less aggressive phenotypes in Triple-Negative breast cancer cells.
Article
Medicine, Research & Experimental
Shyng-Shiou F. Yuan, Yun-Ming Wang, Leong-Perng Chan, Amos C. Hung, Hieu D. H. Nguyen, Yuk-Kwan Chen, Stephen Chu-Sung Hu, Steven Lo, Yen-Yun Wang
Summary: IL-1RA promotes the development of oral squamous cell carcinoma (OSCC) by regulating mitochondrial metabolism-mediated EGFR/JNK activation and SOX2 expression. Inhibition of this mitochondrial metabolic pathway may be a potential therapeutic strategy in OSCC.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Cell Biology
Xiaoli Ji, Tianyong Sun, Shang Xie, Hua Qian, Lixiang Song, Lihua Wang, Hongwei Liu, Qiang Feng
Summary: In this study, it was found that MSCs derived from OSCC promoted OSCC cell metastasis by secreting CXCL8 and upregulating CPNE7 to activate the NF-kappa B pathway. The activation mechanism of CPNE7 includes phosphorylated p65 and I kappa Ba, with silencing of CPNE7 inhibiting metastasis and overexpression promoting this process.
CELL DEATH DISCOVERY
(2021)
Article
Oncology
Jingpeng Liu, Xiao Jiang, Ailing Zou, Zhaoyi Mai, Zhijie Huang, Liying Sun, Jianjiang Zhao
Summary: The study identified a unique circRNA, circIGHG, which is significantly upregulated in oral squamous cell carcinoma (OSCC) and associated with poor prognosis. It promotes OSCC invasion and metastasis by interacting with miR-142-5p and IGF2BP3, with epithelial-mesenchymal transition being the main mechanism. These findings suggest that circIGHG could serve as a biomarker and therapeutic target for early diagnosis and treatment of OSCC.
Article
Biology
Wei Guo, Yuchao Zhang, Shiwei Guo, Zi Mei, Huiping Liao, Hang Dong, Kai Wu, Haocheng Ye, Yuhang Zhang, Yufei Zhu, Jingyu Lang, Landian Hu, Gang Jin, Xiangyin Kong
Summary: Guo, Zhang, Guo et al. analyzed microbiome alterations in different molecular subtypes of PDAC and found distinct signatures in basal-like tumors, characterized by an increase in certain bacteria, such as Acinetobacter, Pseudomonas, and Sphingopyxis. They associated the increased abundance of these bacteria with heightened inflammation and carcinogenic potential, as indicated by pathway analyses.
COMMUNICATIONS BIOLOGY
(2021)
Article
Oncology
Wei-Chen Yen, Kai-Ping Chang, Cheng-Yi Chen, Yenlin Huang, Ting-Wen Chen, Hsing-Wen Cheng, Jui-Shan Yi, Chun-Chia Cheng, Chih-Ching Wu, Chun- Wang
Summary: By comparing DEGs in OSCC-TCGA patients with CNVs identified in OSCC-OncoScan dataset, we found 37 dysregulated candidate genes, 26 of which have been reported in HNSCC. Among 11 novel candidates, MFI2 was identified as the most significant prognostic molecular in OSCC. Knockdown of MFI2 reduced cell viability, migration, and invasion in OSCC cells through modulation of EGF/FAK signaling.
CANCER CELL INTERNATIONAL
(2023)
Article
Oncology
Xudong Zhu, Rongpu Liang, Tianyun Lan, Dongbing Ding, Shengxin Huang, Jun Shao, Zongheng Zheng, Tufeng Chen, Yong Huang, Jianpei Liu, Janak L. Pathak, Hongbo Wei, Bo Wei
Summary: This study found that the specific localization of CD155(+) TAMs in CRC tissue could become a potential therapeutic target for CRC treatment, as it is responsible for M2-phenotype transition, immunosuppression, and tumor progression in CRC.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Oncology
Weijie Zhang, He Jin, Shitong Lou, Haowei Yang, Xiaoyong Dai, Shaohua Ma
Summary: Patient-derived organoids are being increasingly used in basic sciences and precision medicine. However, challenges remain in replicating tumor phenotypes in tumor organoids due to low growth rates and tumor cell impurity. This study introduces a microfluidic droplet encapsulation method that promotes the development of high purity and successful tumor organoids, as compared to non-engineered organoids, using patient-derived samples from esophagus, lung, and colorectal cancers.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Oncology
Zuhair M. Mohammedsaleh, Mamdoh S. Moawadh, Fayez M. Saleh, Mohammed M. Jalal, Abdulaziz S. Al-Otaibi, Nizar H. Saeedi, Rathinasamy Baskaran, Chih-Yang Huang, V. Bharath Kumar
Summary: The study found that Notch1 is aberrantly expressed in oral cancer and is associated with poor prognosis. Notch1 may be a potential biomarker for targeted therapy in oral cancer patients.
Article
Biochemistry & Molecular Biology
Kazuki Kai, Masafumi Moriyama, A. S. M. Rafiul Haque, Taichi Hattori, Akira Chinju, Chen Hu, Keigo Kubota, Yuka Miyahara, Noriko Kakizoe-Ishiguro, Shintaro Kawano, Seiji Nakamura
Summary: It was found that oral squamous cell carcinoma cells promote the differentiation of monocytes into CD206(+) TAMs through the production of PAI-1 and IL-8. Positive correlations were observed among CD206(+), PAI-1(+), and IL-8(+) cells in OSCC sections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Dentistry, Oral Surgery & Medicine
Nuha Mohamed Gaafar, Tarig Al-Hadi Osman, Israa Abdulrahman Ahmed, Mariam Elsheikh, Harsh Dongre, Martha Rolland Jacobsen, Nazar Gafar Mohamed, Siren Fromreide, Ahmed Mohamed Suleiman, Anne Christine Johannessen, Elisabeth Sivy Nginamau, Daniela Elena Costea
Summary: In this study, immune cell infiltrate in oral squamous cell carcinoma (OSCC) was explored using immunohistochemistry. The majority of OSCCs showed low intraepithelial immune infiltration despite heavy immune cell infiltration in tumor-associated stroma. An immunoscore based on combined CD4 and FoxP3 intraepithelial expression may serve as an indicator of advanced tumor progression.
CLINICAL AND EXPERIMENTAL DENTAL RESEARCH
(2022)
Article
Dentistry, Oral Surgery & Medicine
Dipak Sapkota, Sunita Sharma, Tine M. Soland, Paulo H. Braz-Silva, Muy-Teck Teh
Summary: The study found that ACE2, TMPRSS2, and FURIN mRNAs were expressed in normal oral mucosa (NOM) at lower levels compared to other tissues. In oral squamous cell carcinoma (OSCC), except for moderate up-regulation of FURIN, expression levels of ACE2 and TMPRSS2 mRNA remained unchanged or downregulated compared to NOM.
CLINICAL AND EXPERIMENTAL DENTAL RESEARCH
(2022)
Article
Dentistry, Oral Surgery & Medicine
Narmin Sofizadeh, Bodil Bjerkehagen, Tore Solheim, Dipak Sapkota, Tine M. Soland
Summary: This study aimed to evaluate the spectrum and frequency of oral histopathological diagnoses signed out by oral pathologists, as well as compare the diagnoses with the content of the current syllabus in oral pathology. The results showed that a minor revision of the syllabus is needed, and a more clinical related teaching approach should be considered.
EUROPEAN JOURNAL OF DENTAL EDUCATION
(2023)
Article
Dentistry, Oral Surgery & Medicine
Ridhima Das, Maria Justina Roxana Virlan, Victoria Xenaki, Keerthi K. Kulasekara, Ochiba Lukandu, Evelyn Neppelberg, Olav K. Vintermyr, Anne Chr Johannessen, Bogdan Calenic, Daniela Elena Costea
Summary: This study aimed to identify fibroblast-derived factors responsible for oral epithelial differentiation. The results showed that the synergic combination of GM-CSF and KGF regulated major aspects of oral epithelial differentiation, while the terminal stage appeared to be controlled by other unidentified fibroblast-derived factors.
EUROPEAN JOURNAL OF ORAL SCIENCES
(2022)
Article
Oncology
Muy-Teck Teh, Hong Ma, Ying-Ying Liang, Monica Charlotte Solomon, Akhilanand Chaurasia, Ranjitkumar Patil, Satyajit Ashok Tekade, Deepika Mishra, Fatima Qadir, Ji-Yun Stephanie Yeung, Xinting Liu, Safa Kriuar, Ruoqi Zhao, Ahmad Waseem, Iain L. Hutchison
Summary: The study validated a novel multi-gene qPCR test on 535 tissue specimens from the UK, China, and India, demonstrating a rapid minimally invasive method for sensitive oral cancer detection. Further research is needed to determine if this method could improve the management of oral potentially malignant disorders and reduce the burden of oral cancer.
Article
Oncology
Saroj Rajthala, Himalaya Parajuli, Harsh Nitin Dongre, Borghild Ljokjel, Kristin Marie Hoven, Arild Kvalheim, Stein Lybak, Evelyn Neppelberg, Dipak Sapkota, Anne Christine Johannessen, Daniela-Elena Costea
Summary: MiR-138 exhibits heterogeneous expression in cancer-associated fibroblasts (CAFs) in oral squamous cell carcinoma (OSCC). Despite its heterogeneous expression, it suppresses cell motility, collagen contraction ability, and invasion of adjacent cancer cells.
FRONTIERS IN ONCOLOGY
(2022)
Review
Developmental Biology
Maria Justina Roxana Virlan, Daniela Elena Costea, Diana Loreta Paun, Adina Zamfir-Chiru-Anton, Alin Gabriel Sterian, Arsenie Dan Spinu, Victor Nimigean, Vanda Roxana Nimigean
Summary: Changes in the TMJ are common in adults, but not all changes are degenerative. Overloading masticatory forces can lead to disturbed remodeling of the joint structures, and degenerative modifications can occur not only in the condylar process but also in the temporal TMJ region.
ROMANIAN JOURNAL OF MORPHOLOGY AND EMBRYOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Nuha M. Gaafar, Tarig Al-Hadi Osman, Mariam Elsheikh, Israa Abdulrahman Ahmed, Harsh Dongre, Siren Fromreide, Ahmed M. Suleiman, Anne C. Johannessen, Elisabeth S. Nginamau, Daniela-Elena Costea
Summary: This study aimed to evaluate immune cell infiltration patterns at the invasive tumor front (ITF) in oral squamous cell carcinoma (OSCC) patients from Sudan and their association with clinicopathological parameters. The results showed differences in the distribution of immune cells between the tumor center and the ITF, and the expression of PD-L1 at the ITF was identified as an independent prognostic factor for OSCC.
CLINICAL AND EXPERIMENTAL DENTAL RESEARCH
(2022)
Article
Oncology
Divya Ganesh, Amal Dafar, Julia Niklasson, Ingrid Sandberg, Paulo Braz-silva, Dipak Sapkota, Jenny Ohman, Daniel Giglio, Bengt Hasseus
Summary: This study aimed to explore the association between EZH2 expression, immune activation, and cancer transformation in oral leukoplakia (OL). The results showed that EZH2 expression in the epithelium was significantly higher in OL cases that had undergone transformation to oral squamous cell carcinoma (OSCC). Furthermore, high expression of EZH2 in OL epithelium was associated with an increased risk for developing OSCC and correlated with the level of T-cell infiltration.
ANTICANCER RESEARCH
(2023)
Article
Dentistry, Oral Surgery & Medicine
B. Sengueven Tokozlu, D. Sapkota, E. M. Vallenari, O. Schreurs, T. M. Soland
Summary: Oral squamous cell carcinoma of the tongue (OTSCC) is a common and unfavorable malignancy. Overexpression of cortactin is associated with invasion and metastasis of tumor cells. This study investigates the expression pattern and prognostic significance of cortactin in HPV-negative OTSCC, finding that high overall cortactin expression is associated with larger tumor size, distant metastasis, and reduced 5-year survival. Silencing cortactin suppresses the proliferative and invasive abilities of OTSCC cells.
EUROPEAN JOURNAL OF ORAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ridhima Das, Lisa Harper, Kayoko Kitajima, Tarig Al-Hadi Osman, Mihaela Roxana Cimpan, Anne Chr. Johannssen, Salwa Suliman, Ian C. C. Mackenzie, Daniela-Elena Costea
Summary: This study aimed to investigate the differentiation of pluripotent stem cells into oral epithelial cells under molecular signals from the extracellular matrix (ECM). Mouse embryonic stem cells (ESC) of the E14 cell line were cultured in 2D on various matrices to assess epithelial differentiation. The matrices included gelatin, laminin, and ECM synthesized by oral fibroblasts and keratinocytes. ESC grown on these matrices were then transferred to 3D organotypic cultures to further evaluate differentiation into oral epithelia. The results showed that ECM synthesized by oral fibroblasts or keratinocytes induced the expression of cytokeratins, leading to epithelial maturation. The presence of oral fibroblasts in the collagen matrix was necessary for the full differentiation into oral epithelial cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Dentistry, Oral Surgery & Medicine
Ashutosh Kumar Singh, Ragavi Alagarsamy, Rajib Chaulagain, Abanish Singh, Dipak Sapkota, Selvam Thavaraj, Rabindra P. Singh
Summary: This study analyzed the recurrence rate of BRAFv600e+ and BRAFv600e- ameloblastomas and found that females in the BRAFv600e+ group had a higher recurrence rate, suggesting the potential need for BRAFv600e mutation investigation and treatment.
JOURNAL OF ORAL PATHOLOGY & MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Suyog Basnet, Evan Michael Vallenari, Urusha Maharjan, Sunita Sharma, Olaf Schreurs, Dipak Sapkota
Summary: S100A16 is a member of the S100 protein family and is expressed in various human tissues, particularly those rich in epithelial cells. It is differentially expressed in the majority of human cancers and has been implicated in tumorigenesis, including cell proliferation, differentiation, migration, invasion, and epithelial-mesenchymal transition. Additionally, S100A16 expression has prognostic potential in various cancer types.
Article
Biochemistry & Molecular Biology
Svein Erik Moe, Fredrik A. Erland, Siren Fromreide, Stein Lybak, Marianne Brydoy, Harsh N. Dongre, Sophia M. Dhayalan, Daniela-Elena Costea, Olav K. Vintermyr, Hans Jorgen Aarstad
Summary: This study found an association between somatic TP53 mutations and the presence of human papillomavirus (HPV) in tumors of head and neck squamous cell carcinoma (HNSCC) patients. Only 2 of 44 HPV-positive patients had TP53 mutations, while 42 of 60 HPV-negative patients had TP53 mutations. Furthermore, in HPV-negative patients, a higher percentage of somatic TP53 mutations were found in the TP53 R72 polymorphism cohort compared to the TP53 P72 cohort.