Article
Infectious Diseases
Xi Liu, Yuanli Chen, Hui Ouyang, Jian Liu, Xiaoqing Luo, Yayi Huang, Yan Chen, Jinmin Ma, Jinyu Xia, Li Ding
Summary: Combining mNGS with conventional detection methods can increase the detection rate for Mycobacterium tuberculosis complex (MTBC). Additionally, mNGS is able to identify new potential pathogens, aiding in better diagnosis of coinfection.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Medicine, General & Internal
Cheng Lei, Xianglin Zhou, Shuizi Ding, Yingjie Xu, Binyi Yang, Wei Guo, Min Song, Min Yang, Yunan Jia, Hong Luo
Summary: This article reports a case of Legionella gormanii pneumonia diagnosed by metagenomic next-generation sequencing (mNGS). The use of mNGS in diagnosing Legionella infection provides a high-resolution and sensitive assay, especially for non-Legionella pneumophila serogroup 1 strains.
FRONTIERS IN MEDICINE
(2022)
Article
Medicine, General & Internal
Wei Guo, Xiaojian Cui, Qiushi Wang, Yupeng Wei, Yanqing Guo, Tongqiang Zhang, Jianghua Zhan
Summary: This study evaluates the usefulness of mNGS in pathogen detection in BALF specimens from children with CAP. The results show that mNGS is more accurate in detecting bacterial infections and co-infections compared to conventional tests. It can also identify pathogens beyond the scope of CT methods.
FRONTIERS IN MEDICINE
(2022)
Article
Immunology
Jing Zhang, Lin Gao, Chi Zhu, Jiajia Jin, Chao Song, Hang Dong, Zhenzhong Li, Zheng Wang, Yubao Chen, Zhenhua Yang, Yan Tan, Li Wang
Summary: This study evaluated the clinical value of mNGS by Illumina and Nanopore in pathogen detection in suspected CAP patients, showing that Nanopore detected a wider range of species, especially in viruses, fungi, and mycobacteria, with a shorter TAT. However, it had similar diagnostic performance and treatment guidance compared to Illumina.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Medicine, Research & Experimental
Xiaohui Zhang, Yuhong Qin, Wei Lei, Jian-An Huang
Summary: The diagnostic accuracy of metagenomic next-generation sequencing (mNGS) is superior to conventional microbiological tests (CMTs) for severe community-acquired pneumonia (SCAP) in immunocompromised patients. mNGS has advantages in detecting polymicrobial infections and identifying unexpected pathogens. Therefore, mNGS can be considered as an important diagnostic method for SCAP.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2023)
Article
Immunology
He Sun, Feilong Wang, Ming Zhang, Xiaoyong Xu, Miaomiao Li, Wei Gao, Xiaodong Wu, Huize Han, Qin Wang, Gehong Yao, Zheng Lou, Han Xia, Yi Shi, Qiang Li
Summary: This study evaluates the value of metagenomic next-generation sequencing (mNGS) of bronchoalveolar lavage fluid (BALF) in the diagnosis of Pneumocystis jirovecii pneumonia (PJP) and its mixed infection in non-human immunodeficiency virus (HIV) immunosuppressed patients. The study found that BALF-mNGS had a higher positive detection rate for various pathogens, especially mixed infections, compared to conventional methods. BALF-mNGS had higher sensitivity and specificity for the diagnosis of PJP compared to traditional methods, and its detection time was significantly shorter.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Wenhua Deng, Huan Xu, Yabin Wu, Jie Li
Summary: BALF mNGS improves the detection rate of pediatric pneumonia, especially in mixed infections. However, the diagnostic performance of BALF mNGS varies according to the type of pathogen and cannot replace CMTs. A combination of mNGS and CMTs may be the best diagnostic strategy.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Aimei Yang, Chen Chen, Yan Hu, Guilang Zheng, Peiling Chen, Zhiwei Xie, Huifeng Fan, Yueyu Sun, Peiqiong Wu, Wenhui Jiang, Chun Wang, Jingwen Zhang, Dongwei Zhang, Jing Wang, Xiaoyin Hu, Han Xia, Genquan Yin, Yuxiong Guo
Summary: The study evaluates the performance of metagenomic next-generation sequencing (mNGS) using bronchoalveolar lavage fluid (BALF) samples for diagnosing pneumonia in children. The results show that mNGS has a higher detection rate and coincidence rate compared to conventional methods. Bacteria, viruses, and mixed infections were common in children with pneumonia. mNGS of BALF samples can improve the diagnosis and prognosis of pneumonia in children.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Yi-Rong Zheng, Shi-Hao Lin, Yu-Kun Chen, Hua Cao, Qiang Chen
Summary: mNGS is a valuable tool to determine the etiology of infants with severe pneumonia after congenital heart surgery. It can improve the sensitivity of pathogen detection and enhance the accuracy of treatment outcomes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Minmin Lin, Kongqiu Wang, Lidi Qiu, Yingjian Liang, Changli Tu, Meizhu Chen, Zhenguo Wang, Jian Wu, Yiying Huang, Cuiyan Tan, Qijiu Chen, Xiaobin Zheng, Jing Liu
Summary: Tropheryma whipplei, the bacterium associated with Whipple's disease, has been detected in the saliva and feces of healthy individuals. This study retrospectively analyzed BALF samples and found the presence of T. whipplei in lung diseases, including tuberculosis and lung tumors. The results suggest that T. whipplei may be a potential factor in certain lung diseases.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Caiyan Zhang, Tingyan Liu, Yixue Wang, Weiming Chen, Jing Liu, Jinhao Tao, Zhengzheng Zhang, Xuemei Zhu, Zhenyu Zhang, Meixiu Ming, Mingbang Wang, Guoping Lu, Gangfeng Yan
Summary: This study used mNGS to characterize the microbiome of BALF in children with severe pneumonia and identified potentially pathogenic microorganisms. The study found that bacterial combined with viral or fungal infections may be present in the BALF of patients with severe pneumonia, and viral or fungal infections are associated with greater disease severity and death.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Shenglin Chen, Chenrui Hou, Yutong Kang, Dan Li, Jianrong Rong, Zhenjun Li
Summary: The aim of this study was to explore the clinical utility of metagenomic next-generation sequencing (mNGS) in diagnosing community-acquired pneumonia (CAP) and to reveal resistome differences in bronchoalveolar lavage fluid (BALF) from CAP patients with varying severity of admission based on Pneumonia Patient Outcomes Research Team (PORT) risk classes. The diagnostic sensitivities of mNGS and conventional testing for the detection of pathogens in BALF from CAP patients were 96.6% and 30.5%, respectively. Significant differences were found in the overall relative abundance of resistance genes and composition of resistance genes among different PORT risk classes.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Pediatrics
Yupeng Wei, Tongqiang Zhang, Yuting Ma, Jisi Yan, Jianghua Zhan, Jun Zheng, Yongsheng Xu
Summary: Rapid and accurate identification of pathogens is crucial for the treatment of severe community-acquired pneumonia (SCAP) in children. Metagenomic Next-generation sequencing (mNGS) has been applied in pathogen detection, but the overall evaluation of its application in children with SCAP is lacking.
ITALIAN JOURNAL OF PEDIATRICS
(2023)
Article
Infectious Diseases
Yirui Xie, Bohao Dai, Xiaotang Zhou, Huiting Liu, Wei Wu, Fei Yu, Biao Zhu
Summary: mNGS has a higher diagnostic accuracy for HIV-infected patients with suspected mixed pneumonia, especially in detecting fungal pathogens. It can identify more co-pathogens in mixed infections compared to conventional microbiological tests (CMTs). Early performance of bronchoscopy and mNGS are recommended in HIV-infected patients with low CD4 T cell counts and long duration of symptoms.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Infectious Diseases
Xuefang Li, Zhijun Li, Jian Ye, Wu Ye
Summary: Based on the analysis of 9 studies, the results showed that metagenomic next-generation sequencing (mNGS) has high accuracy for the diagnosis of Pneumocystis jirovecii pneumonia (PJP). It is a promising tool for assessing the condition of PJP in both immunocompromised and non-HIV patients.
BMC INFECTIOUS DISEASES
(2023)
Article
Chemistry, Multidisciplinary
Tianqi Wang, Yanan Fu, Shengjie Sun, Chenyi Huang, Yunfei Yi, Junqing Wang, Yang Deng, Meiying Wu
Summary: This review summarizes the important role of exosomes, such as extracellular vesicles, in cell-to-cell communication, especially in the tumor microenvironment, where exosomes serve as key pathways for the transfer of proteins, nucleic acids, and small molecules between different types of cells. The applications of exosomes as drug delivery systems in cancer therapy are classified based on the cell source of the exosomes, including normal cells, immune cells, and tumor cells. Different modifications of exosomes and methods of drug loading are presented. Finally, the challenges that hinder the clinical translation of exosomes are discussed.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Mingming Han, Jingjing Yao, Jiwu Huang, Yan Tang, Xianwen Wu, Bingan Lu, Jiang Zhou
Summary: Aqueous rechargeable Zn//MnO2 batteries are a promising candidate for future energy storage systems due to their economic and environmental advantages. However, poor reaction kinetics and capacity degradation hinder the performance of Zn//MnO2 batteries. This study demonstrates that defects engineering and phase transformation can enhance ion/charge transfer kinetics and structural stability, leading to a high discharge capacity of 285 mAh/g and capacity retention of 81% after 500 cycles in the aqueous Zn//A-MnO2 system.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Rui Liu, Cong Luo, Zhiqing Pang, Jinming Zhang, Shaobo Ruan, Meiying Wu, Lei Wang, Tao Sun, Nan Li, Liang Han, Jinjin Shi, Yuanyu Huang, Weisheng Guo, Shaojun Peng, Wenhu Zhou, Huile Gao
Summary: Decades have passed since the approval of the first nanoparticles-based medicine for human cancer treatment, and research and development in nanoparticles for drug delivery have been continuing. Today, there have been significant advancements in nanoparticles, including liposomes, solid lipid nanoparticles, inorganic nanoparticles, micelles, nanovaccines, and nano-antibodies. These nanoparticles have shown great potential in the treatment and diagnosis of challenging diseases such as cancer and neurodegenerative diseases, making them promising drug carriers and therapeutic agents in the future.
CHINESE CHEMICAL LETTERS
(2023)
Review
Cell Biology
Yonghui Lv, Meiying Wu, Zhe Wang, Junqing Wang
Summary: Ferroptosis is a regulated cell death caused by iron-dependent lipid peroxide accumulation. The imbalance of intracellular oxidative systems and antioxidant systems is the main cause, regulated by a complex network. Cancer cells with resistance to apoptosis and chemotherapeutics are prone to ferroptosis, making it a potential strategy for eliminating cancer cells. Ferroptosis is also linked to other diseases such as neurological disorders and ischemia-reperfusion injury, providing new insights for clinical treatment.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Chemistry, Physical
Baohua Zhang, Meiying Wu, Liang Zhang, Yun Xu, Weidong Hou, Huazhang Guo, Liang Wang
Summary: In this study, a one-step carbonization strategy was designed to synthesize Co nanoparticles supported on N-doped carbon nanotubes (Co/CNT) with excellent bifunctional electrocatalytic performance. The Co/CNT material exhibited long-term durability in the oxygen reduction reaction and oxygen evolution reaction, and was successfully applied in a rechargeable Zn-air battery.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Daiyun Xu, Xu Chen, Yongxiao Li, Zhidong Chen, Wanting Xu, Xinpei Wang, Yonghui Lv, Zhe Wang, Meiying Wu, Gang Liu, Junqing Wang
Summary: Nanodiscs (NDs) formed by apoA-I peptides can be designed with different diameters through varying factors such as the number of tandem repeats, sequence composition, and lipid-to-APA ratio. Proline is dispensable for APA-ND formation, but its substitution can induce smaller NDs and larger NDs depending on the lipid ratio. Proline-tryptophan residues play a dominant role in forming larger NDs. Molecular simulation analysis shows that enriched basic and acidic residues in APAs enhance the structural stability of APA-NDs through hydrogen-bond and salt bridge networks.
Article
Engineering, Biomedical
Chenyi Huang, Jia Tang, Yuanqi Liu, Ting Chen, Junyang Qi, Shengjie Sun, Huisong Hao, Weiwei Zeng, Jing Zhao, Meiying Wu
Summary: This study developed a multifunctional nanocatalyst Cu-doped polypyrrole (CuP) as an intelligent nanoplatform to deliver the NO precursor BNN6 and specifically release NO in tumors. Through the synergistic effect of hyperthermia, oxidative damage, and NO burst, almost complete tumor elimination is achieved in vivo.
ACTA BIOMATERIALIA
(2023)
Article
Microbiology
Xuejiao Hu, Yunhu Zhao, Peng Han, Suling Liu, Weijiang Liu, Cong Mai, Qianyun Deng, Jing Ren, Jiajie Luo, Fangyuan Chen, Xuefeng Jia, Jing Zhang, Guanhua Rao, Bing Gu
Summary: Multidrug-resistant (MDR) bacteria are a significant public health concern, and the current antibiotic susceptibility testing (AST) methods are time-consuming and can lead to delays in treatment and increased mortality. In this study, we developed a machine learning model using Acinetobacter baumannii as an example to explore a fast AST method using metagenomic next-generation sequencing (mNGS) data. The model accurately predicted antimicrobial resistance (AMR) signatures and antibacterial phenotypes, and the mNGS-based AST approach reduced reporting time significantly compared to culture-based AST methods. This model has the potential to be used for rapid genotypic AST and improve rational antimicrobial usage.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Biochemical Research Methods
Yunfei Yi, Chan Feng, Mian Yu, Lin Mei, Meiying Wu, Wei Tao
Summary: Efficient gene delivery in an integrated drug delivery system is crucial for multimodal antitumor therapy. This study presents a protocol for constructing a peptide-based siRNA delivery system that achieves tumor vascular normalization and gene silencing in 4T1 cells. The system highlights four major steps and has the potential to silence gene expression and perform other treatments based on different peptide segments.
Article
Chemistry, Multidisciplinary
Sheng Hong, Zhangwen Peng, Meiying Wu, Yichu Nie, Yunfei Yi, Hui Cai, Xian-Zheng Zhang
Summary: Extraction of multifunctional particles from human hair provides an ingenious tactic for the design and development of sunscreens, which can effectively protect skin from UV radiation and prevent potential toxicity.
Article
Microbiology
Bing Liu, Jianpeng Gao, Xue Fei Liu, Guanhua Rao, Jiajie Luo, Peng Han, Weiting Hu, Ze Zhang, Qianqian Zhao, Lizhong Han, Zhi Jiang, Min Zhou
Summary: This study successfully achieved the direct prediction of carbapenem resistance in Pseudomonas aeruginosa through the selection of key gene features and construction of machine learning models. The prediction was validated in clinical samples using metagenomic sequencing technology, providing assistance in timely treatment and surveillance of carbapenem-resistant Pseudomonas aeruginosa.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Correction
Chemistry, Multidisciplinary
Ting Chen, Weiwei Zeng, Yuanqi Liu, Mian Yu, Chenyi Huang, Zhaoqing Shi, Chuchu Lin, Jia Tang, Lin Mei, Meiying Wu
Correction
Multidisciplinary Sciences
Yun Zhou, Mian Yu, Changjun Tie, Yang Deng, Junqing Wang, Yunfei Yi, Fan Zhang, Chenyi Huang, Hairong Zheng, Lin Mei, Meiying Wu
Review
Pharmacology & Pharmacy
Zhe Wang, Xinpei Wang, Wanting Xu, Yongxiao Li, Ruizhi Lai, Xiaohui Qiu, Xu Chen, Zhidong Chen, Bobin Mi, Meiying Wu, Junqing Wang
Summary: Biomimetic delivery systems (BDSs) are a groundbreaking paradigm in nanomedicine inspired by the designs of biological systems. They offer targeted delivery, minimized side effects, and enhanced therapeutic outcomes. However, their translation to clinical applications faces challenges in reproducibility, physiological stability, and rigorous efficacy and safety evaluations. Collaborative interdisciplinary research and reevaluation of regulatory frameworks are necessary for successful clinical translation.
Article
Cell Biology
Yue Li, Meiying Wu, Xinyu Xing, Xingxing Li, Congchong Shi
Summary: This study investigates the repair process of dentin-pulp injuries and the role of the Wnt10a/ss-β-catenin signaling pathway. The activation of this pathway was found to promote proliferation and odontoblast differentiation of dental pulp stem cells. The findings contribute to a better understanding of the mechanisms involved in dentin-pulp injury repair and provide new insights for future studies on tooth regeneration.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2023)
