Article
Biotechnology & Applied Microbiology
Yao Zu, Sujuan Hong, Congcong Yin, Yu Luo, Chen Xu, Jianhong Li
Summary: Cyanobacterial alkaline phosphatases (APases) play a crucial role in phosphorus utilization in freshwater. Tracking the distribution of APases can provide insights into the physiological response of phytoplankton to phosphorus nutrition. In this study, an immunoassay for PhoX detection was developed, and specific fluorescent-labeled nanobodies were constructed for visualization and evaluation of PhoX expression levels in Microcystis.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
G. Katsipis, V. Tsalouxidou, E. Halevas, E. Geromichalou, G. Geromichalos, A. A. Pantazaki
Summary: The study investigated a novel V(IV)-curcumin-bipyridine complex as a potential inhibitor of ALP activity and bacterial biofilm. The results showed that V-Cur inhibited ALP activity in a dose-dependent manner and had an inhibitory effect on bacterial biofilm formation. This suggests that V-Cur has potential as a therapeutic agent against bacterial infections and ALP-associated diseases.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Sujuan Hong, Qianhui Pan, Siyu Chen, Yao Zu, Chongxin Xu, Jianhong Li
Summary: This study confirmed the presence of a putative phoX gene in M. aeruginosa PCC7806, which showed phosphodiesterase and phosphomonoesterase activities, responding to different levels of environmental phosphorus. The PhoX in Microcystis was activated by Mg2+ and other ions, while being inhibited by Ni2+, indicating conservation and unique characteristics of PhoX in this genus.
Article
Soil Science
Xiaodong Chen, Leo M. Condron, Kari E. Dunfield, Steven A. Wakelin, Lijun Chen
Summary: The study found that grassland afforestation significantly decreased soil organic phosphorus and microbial biomass phosphorus, while increasing inorganic phosphorus bioavailability. Although there were no significant differences between tree species, the separation of phoD and phoX gene bacterial communities associated with afforestation was more influenced by soil moisture and pH.
SOIL BIOLOGY & BIOCHEMISTRY
(2021)
Article
Microbiology
Kate R. Fortney, Sara N. Smith, Julia J. van Rensburg, Julie A. Brothwell, Jessi J. Gardner, Barry P. Katz, Nagib Ahsan, Adam S. Duerfeldt, Harry L. T. Mobley, Stanley M. Spinola
Summary: This study suggests that chemical activation of the CpxRA system may be a viable strategy for treating infections caused by uropathogenic Escherichia coli (UPEC). The most potent compound significantly reduced bacterial recovery in the urine and downregulated multiple proteins involved in UPEC virulence.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Maria N. Siniagina, Maria Markelova, Eugenia A. Boulygina, Alexander Laikov, Dilyara R. Khusnutdinova, Sayar R. Abdulkhakov, Natalia A. Danilova, Alfiya H. Odintsova, Rustam A. Abdulkhakov, Tatyana Grigoryeva
Summary: Crohn's disease is characterized by chronic inflammation in the gastrointestinal tract, where an imbalance in gut microbiota, particularly Escherichia coli, plays a significant role in its pathogenesis. Analysis of E. coli strains from CD patients and healthy subjects revealed genetic similarities in virulence and antibiotic resistance, suggesting complex bacterial interactions within the gut that may impact the host's intestinal health.
Article
Microbiology
Leo van Overbeek, Marie Duhamel, Stefan Aanstoot, Carin Lombaers van der Plas, Els Nijhuis, Leo Poleij, Lina Russ, Patricia van der Zouwen, Beatriz Andreo-Jimenez
Summary: The study aimed to investigate the transmission route of E. coli strain 0611 via manure, soil, and plant root zones to above-soil plant compartments. It was found that the strain accumulated in the root zones of both plant species, but was rarely detected in the leaves.
Article
Environmental Sciences
Jian Zhou, Xiaoxia Han, Boqiang Qin, Guangwei Zhu
Summary: This study conducted a mesocosm experiment in Lake Taihu to investigate the effects of wind waves on alkaline phosphatase activity fractions. The results showed that wind-driven waves induced the release of alkaline phosphatase and phosphorus from the sediment, enhancing phytoplanktonic alkaline phosphatase activity, while bacterial and dissolved alkaline phosphatase activity decreased in wind-wave conditions.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2021)
Article
Ecology
Abhishek Srivastava, Daniel E. M. Saavedra, Blair Thomson, Juan A. L. Garcia, Zihao Zhao, Wayne M. Patrick, Gerhard J. Herndl, Federico Baltar
Summary: The study revealed that marine APase enzyme exhibits promiscuous behavior, performing not only P-monoesterase activity but also P-diesterase, P-triesterase, and sulfatase activities. APase is highly efficient as a P-monoesterase at high substrate concentrations, while promiscuous activities are more efficient at low substrate concentrations.
Review
Chemistry, Multidisciplinary
Meng Li, Bhaskar Gurram, Shan Lei, Nicholas T. Blum, Peng Huang, Jing Lin
Summary: Phosphatase is crucial in many biological functions, with alkaline phosphatase (ALP) serving as a key prognostic indicator for various diseases, though current detection methods are limited to in vitro colorimetric techniques. Fluorescence imaging provides a reliable way to monitor real-time changes in ALP levels, with research focusing mainly on ALP imaging probes grouped by their luminescence mechanisms. Challenges and future prospects for phosphatase probes were also discussed.
CHINESE CHEMICAL LETTERS
(2021)
Article
Food Science & Technology
Smriti Shrestha, Sanam Pudasaini, Basant Giri
Summary: Novel paper-based assays were developed for alkaline phosphatase (ALP), starch, and urea using specific reagents. The assays showed wide working ranges and low detection limits. The newly developed methods were applied to test pasteurisation and adulteration of milk samples.
INTERNATIONAL DAIRY JOURNAL
(2022)
Article
Multidisciplinary Sciences
Nicola Pellicciotta, Matteo Paoluzzi, Dario Buonomo, Giacomo Frangipane, Luca Angelani, Roberto Di Leonardo
Summary: By modulating bacterial motility with light, researchers have found a way to control the transport direction and speed of microscopic particles by adjusting the active pressure gradients. This discovery provides new insights into the potential use of active pressure for controlled transport of microscopic objects.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Helena Massana-Cid, Claudio Maggi, Giacomo Frangipane, Roberto Di Leonardo
Summary: Optical feedback can be used to confine and gather bacteria, forming dense high-activity regions. This method is general and scalable, making it versatile for microengineering applications and studying non-equilibrium phenomena in active systems.
NATURE COMMUNICATIONS
(2022)
Article
Nutrition & Dietetics
Zhenyu Wang, Jingjing Li, Lingyan Ma, Xiangdong Liu, Hong Wei, Yingping Xiao, Shiyu Tao
Summary: Neonatal diarrhea is a severe disease in humans and pigs, but the role of gut virome has not been studied. Using metagenomic sequencing, the viral community of diarrheal and healthy neonatal piglets was characterized. The viral community of diarrheal piglets showed higher heterogeneity and elevated abundance of Myoviridae. Phages infecting Proteobacteria, especially E. coli, were dominant in neonatal diarrheal piglets. Genus E. coli- and genus listeria-infecting bacteriophages were identified as key biomarkers for discriminating between neonatal diarrheal piglets and healthy controls. These findings expand our understanding of the relationship among phages, bacteria, and diarrhea, and may aid in the development of therapeutics for neonatal diarrhea prevention and treatment.
Article
Materials Science, Biomaterials
Lauren Gwynne, George T. Williams, Kai-Cheng Yan, Bethany L. Patenall, Jordan E. Gardiner, Xiao-Peng He, Jean-Yves Maillard, Tony D. James, Adam. C. Sedgwick, A. Toby. A. Jenkins
Summary: Alkaline phosphatase (ALP) is an important enzyme biomarker with potential in detecting pathogenic bacteria. Research has shown that ALP has colorimetric and fluorescent responses towards Staphylococcus aureus and demonstrates selectivity over other bacteria. Encapsulation of ALP in hydrogels can be used as smart wound dressings for monitoring and treating wound infections.
BIOMATERIALS SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Seong Keun Kim, Paul K. Yoon, Soo-Jung Kim, Seung-Gyun Woo, Eugene Rha, Hyewon Lee, Soo-Jin Yeom, Haseong Kim, Dae-Hee Lee, Seung-Goo Lee
MICROBIAL BIOTECHNOLOGY
(2020)
Article
Chemistry, Analytical
Hyewon Lee, Jiseok Lee, Seung-Goo Lee, Patrick S. Doyle
ANALYTICAL CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Kwang-Hyun Park, Sungchul Kim, Su-Jin Lee, Jee-Eun Cho, Vinod Vikas Patil, Arti Baban Dumbrepatil, Hyung-Nam Song, Woo-Chan Ahn, Chirlmin Joo, Seung-Goo Lee, Victoria Shingler, Eui-Jeon Woo
NATURE COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Kil Koang Kwon, Haseong Kim, Soo-Jin Yeom, Eugene Rha, Jinju Lee, Hyewon Lee, Dae-Hee Lee, Seung-Goo Lee
Summary: Genetic circuits utilize various bio-elements, such as enzymes and regulatory cassettes, for quantitative measurement of enzyme activity, metabolic engineering, and dynamic regulation of microbial cells. Antagonists and inhibitors were applied in this study to achieve a dynamic detection range in a genetic circuit, with the antagonist functioning similarly to a resistor in an electrical logic circuit. These biological resistors in genetic circuits provide a rapid and precise control over variable outputs with minimal circuit configuration.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Thien-Kim Le, Su-Bin Ju, Hye-Won Lee, Jin-Young Lee, So-Hyung Oh, Kil-Koang Kwon, Bong-Hyun Sung, Seung-Goo Lee, Soo-Jin Yeom
Summary: The study focused on the direct evolution of Mdh from Lysinibacillus xylanilyticus using a transcriptional factor-based biosensor, leading to the isolation of mutants with high catalytic activity at very low methanol concentrations. These mutants showed significant improvements in methanol conversion efficiency and have the potential to be used in engineering synthetic methylotrophy and enhancing methanol conversion to various useful products.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Hyun Seung Lim, Seong Keun Kim, Seung-Gyun Woo, Tae Hyun Kim, Soo-Jin Yeom, Wonshik Yong, Yoon-Joo Ko, Soo-Jung Kim, Seung-Goo Lee, Dae-Hee Lee
Summary: A novel bisabolol synthase (CcBOS) was identified in globe artichoke, showing higher catalytic efficiency and production rates than previously reported synthases. In fed-batch fermentation, CcBOS achieved the highest reported bisabolol titer to date, indicating potential for economically viable industrial production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Engineering, Environmental
Hyeong-Woo Kim, Jin Hui Jo, Ye-Bin Kim, Thien-Kim Le, Chul-Woong Cho, Chul-Ho Yun, Won Seok Chi, Soo-Jin Yeom
Summary: This study identified two new bacterial strains, Pseudomonas lini JNU01 and Acinetobacter johnsonii JNU01, with high biodegradation efficiency towards PS. Through bioinformatics and transcriptional analysis, the involvement of alkane-1-monooxygenase (AlkB) in PS biodegradation was confirmed. These findings provide significant insights into the potential of Pseudomonas sp. and Acinetobacter sp. as decomposers of PS.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Hyewon Lee, Ji In Baek, Jin-Young Lee, Jiyeong Jeong, Haseong Kim, Dae-Hee Lee, Dong-Myung Kim, Seung-Goo Lee
Summary: This study demonstrated a highly flexible and efficient modular approach for converting methane to mevalonate using a synthetic consortium of methanotrophs and heterotrophs. The platform achieved successful production of mevalonate through optimized culture conditions and engineered strains, showing high stability and reproducibility. This versatile system has the potential for various value-added processes and applications.
METABOLIC ENGINEERING
(2021)
Review
Biotechnology & Applied Microbiology
Soo-Jin Yeom, Thien-Kim Le, Chul-Ho Yun
Summary: Plastic contamination poses threats to ecosystems and wildlife species, making sustainable plastic waste management crucial. Microbial degradation of plastics, utilizing engineered P450 enzymes, can be an effective solution. The proposal suggests using these enzymes to biodegrade polyethylene and incorporating them into synthetic bacteria for plastic degradation.
TRENDS IN BIOTECHNOLOGY
(2022)
Review
Plant Sciences
Yun Ju Choi, Kseniia Zaikova, Soo-Jin Yeom, Yeong-Su Kim, Dong Wook Lee
Summary: Cytosolic lipid droplets (LDs) mainly contain neutral lipids and are considered energy reserves. TAG synthesis in plants involves the cooperation of plastids and the endoplasmic reticulum, with several proteins playing important roles in LD formation. Oleosin is critical for LD integrity and stability, while SDP1 is responsible for mobilizing TAGs in LDs.
Review
Biochemistry & Molecular Biology
Kyung-Chul Shin, Min-Ju Seo, Yeong-Su Kim, Soo-Jin Yeom
Summary: This article discusses the β-carotene oxygenases (BCOs) from animals, plants, and bacteria, and elaborates on the recent progress in understanding their functions, biochemical properties, substrate specificity, and enzyme activities for the production of retinoids in whole-cell conditions. The integration of BCOs into retinal biosynthetic bacterial systems is proposed to improve the performance of retinal production.
Article
Agriculture, Multidisciplinary
Yeon-Ju Jeong, Pil-Won Seo, Min-Ju Seo, Su-Bin Ju, Jeong-Sun Kim, Soo-Jin Yeom
Summary: Methanol-derived formaldehyde can be used as a versatile building block for the production of 2-keto-4-hydroxyacid derivatives. Pseudomonas aeruginosa PAO1 aldolase (PaADL) and methanol dehydrogenase (MDH) were used to enzymatically produce 2-keto-4-hydroxybutyrate from pyruvate and methanol. This simple process provides a green method for the production of 2-keto-4-hydroxyacid derivatives.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Engineering, Environmental
Seung-Do Yun, Chang Oh Lee, Hyun-Woo Kim, Seong Jin An, Seongmin Kim, Min-Ju Seo, Chungoo Park, Chul-Ho Yun, Won Seok Chi, Soo-Jin Yeom
Summary: The isolation of Bacillus species from a landfill site has provided a potential method for the decomposition of polyethylene (PE) using microorganisms. Bacillus thuringiensis JNU01 showed the highest cell growth rate in PE media and effectively decomposed PE to use as a sole carbon source. The presence of new chemical functional groups and physical damage on the surface of the PE film after treatment with B. thuringiensis JNU01 confirmed its potential as a PE decomposer.
ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
(2023)
Article
Biotechnology & Applied Microbiology
Tae Hyun Kim, Seung-Gyun Woo, Seong Keun Kim, Byeong Hyeon Yoo, Jonghyeok Shin, Eugene Rha, Soo Jung Kim, Kil Koang Kwon, Hyewon Lee, Haseong Kim, Hee-Taek Kim, Bong-Hyun Sung, Seung-Goo Lee, Dae-Hee Lee
Summary: In this study, possible biosynthetic pathways for levulinic acid (LA) production from lignin and poly(ethylene terephthalate) were proposed. A genetically encoded biosensor responsive to LA was also created, which can be used for screening and evolving the LA biosynthesis pathway genes. The biosensor showed a linear correlation between fluorescence intensity and LA concentration in the range of 0.156-10 mM LA when expressed in Pseudomonas putida KT2440.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2023)