期刊
JOURNAL OF GENERAL VIROLOGY
卷 101, 期 10, 页码 1085-1089出版社
MICROBIOLOGY SOC
DOI: 10.1099/jgv.0.001469
关键词
SARS-CoV; coronavirus; overlapping gene; ORF3c; 3c; sarbecovirus
资金
- Wellcome Trust [106207]
- European Research Council [646891]
- European Research Council (ERC) [646891] Funding Source: European Research Council (ERC)
Identification of the full complement of genes in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a crucial step towards gaining a fuller understanding of its molecular biology. However, short and/or overlapping genes can be difficult to detect using conventional computational approaches, whereas high-throughput experimental approaches - such as ribosome profiling - cannot distinguish translation of functional peptides from regulatory translation or translational noise. By studying regions showing enhanced conservation at synonymous sites in alignments of SARS-CoV-2 and related viruses (subgenus Sarbecovirus) and correlating the results with the conserved presence of an open reading frame (ORF) and a plausible translation mechanism, a putative new gene - ORF3c - was identified. ORF3c overlaps ORF3a in an alternative reading frame. A recently published ribosome profiling study confirmed that ORF3c is indeed translated during infection. ORF3c is conserved across the subgenus Sarbecovirus, and encodes a 40-41 amino acid predicted transmembrane protein.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据