Review
Genetics & Heredity
Iuri Viotti Perisse, Zhiqiang Fan, Galina N. Singina, Kenneth L. White, Irina A. Polejaeva
Summary: Accelerated development of novel CRISPR/Cas9-based genome editing techniques allows for precise modifications in the mammalian genome, making it the preferred method for introducing gene alterations in livestock species. The expanding list of new CRISPR/Cas9-based genome editing tools includes methods to improve efficiency and specificity, gene regulation, base editing, and epigenetic modifications. Additionally, the advantages and disadvantages of two primary methods for producing gene-edited farm animals, as well as the agricultural and biomedical applications of gene editing technology, are discussed.
FRONTIERS IN GENETICS
(2021)
Review
Agriculture, Dairy & Animal Science
Julia Popova, Victoria Bets, Elena Kozhevnikova
Summary: Genome editing has practical applications in farm animals, improving production traits, economic value, and disease resistance. It also has potential in biomedical research and drug production, as well as xenograft donors. Recent advancements in site-specific nucleases and embryological delivery methods have revolutionized transgenesis, providing efficient and reliable tools for genome engineering in agriculture.
Article
Multidisciplinary Sciences
Jenny-Helena Soellner, Hendrik Johannes Sake, Antje Frenzel, Rita Lechler, Doris Herrmann, Walter Fuchs, Bjoern Petersen
Summary: The domesticated pig is a useful animal model for biomedical research and genome editing tools have contributed to developing porcine models for human diseases. By using the CRISPR-Cas9 system, porcine embryos or somatic cells can be genetically modified to generate the desired genotype. The study shows that Cas9 expressing pigs can be used to induce desired genomic modifications to model human diseases or further evaluate in vivo gene therapy approaches.
Review
Biochemistry & Molecular Biology
Si-Wei Wang, Chao Gao, Yi-Min Zheng, Li Yi, Jia-Cheng Lu, Xiao-Yong Huang, Jia-Bin Cai, Peng-Fei Zhang, Yue-Hong Cui, Ai-Wu Ke
Summary: The CRISPR/Cas9 gene editing technology has made significant progress in oncological research, providing a powerful tool for exploring the mechanism of cancer, establishing tumor models, and studying drug targets.
Review
Materials Science, Multidisciplinary
Shao Wei Hu, Tao Ding, Honghai Tang, Huiping Guo, Wenguo Cui, Yilai Shu
Summary: With the advancement of genome editing techniques, gene therapy is increasingly being used for the treatment of various human diseases. Nanomaterials have shown great potential in improving the efficiency and safety of gene editing in gene therapy. This review introduces three gene editing tools, with a particular focus on the development and optimization of the CRISPR system. It also discusses the recent application of nanobiomaterials in gene therapy, along with the prospects and remaining challenges. Lastly, future directions for gene editing tools, nanobiomaterial vectors, and gene therapy are presented.
Article
Cell Biology
Kensuke Yamashita, Hoshie Iriki, Yoichiro Kamimura, Tetsuya Muramoto
Summary: This article discusses the development of CRISPR/Cas9-based techniques for generating gene mutants in Dictyostelium, along with the creation of new tools such as double knockouts and drug-inducible knockouts. The data demonstrate the feasibility and efficiency of using CRISPR/Cas9 technology to rapidly generate suitable mutants for studying gene function in Dictyostelium.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Gertje Eta Leony Petersen, Jaap B. Buntjer, Fiona S. Hely, Timothy John Byrne, Andrea Doeschl-Wilson
Summary: Recent breakthroughs in gene-editing technologies offer unprecedented opportunities for controlling future epidemics in farm animals, although the theoretical framework for estimating epidemiological effects is lacking. This study developed modeling approaches to investigate the impact of gene editing on infectious disease prevalence in farmed animal populations. The results suggest that disease elimination may be achievable within 3 to 6 years if gene editing is complemented with widespread and effective vaccination.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biology
Ganna Reint, Zhuokun Li, Kornel Labun, Salla Keskitalo, Inkeri Soppa, Katariina Mamia, Eero Tolo, Monika Szymanska, Leonardo A. Meza-Zepeda, Susanne Lorenz, Artur Cieslar-Pobuda, Xian Hu, Diana L. Bordin, Judith Staerk, Eivind Valen, Bernhard Schmierer, Markku Varjosalo, Jussi Taipale, Emma Haapaniemi
Summary: Precision CRISPR gene editing can be improved by fusing DNA repair proteins with Cas9, with different fusion proteins showing varying effects based on cell type and genomic site. Optimization is necessary to account for the diverse factors contributing to locus-specific genome editing outcomes.
Review
Biotechnology & Applied Microbiology
Congting Guo, Xiaoteng Ma, Fei Gao, Yuxuan Guo
Summary: Gene editing involves precise changes to specific nucleic acid sequences. The CRISPR/Cas9 system has revolutionized gene editing, making it efficient, convenient, and programmable. However, off-target effects remain a major concern, leading to unexpected alterations in the genome. Various methods have been developed to detect and mitigate these off-target effects, advancing the precision of CRISPR/Cas9 derivatives. This review summarizes these technological advancements and discusses the challenges in managing off-target effects for future gene therapy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
J. Boutin, J. Rosier, D. Cappellen, F. Prat, J. Toutain, P. Pennamen, J. Bouron, C. Rooryck, J. P. Merlio, I. Lamrissi-Garcia, G. Cullot, S. Amintas, V. Guyonnet-Duperat, C. Ged, J. M. Blouin, E. Richard, S. Dabernat, F. Moreau-Gaudry, A. Bedel
Summary: The study reveals the genotoxicity caused by CRISPR-Cas9 leading to loss of heterozygosity changes, while also highlighting the potential safety concerns of this technology for gene therapy.
NATURE COMMUNICATIONS
(2021)
Article
Plant Sciences
Guo-Qing Song, Grace Urban, John T. Ryner, Gan-Yuan Zhong
Summary: This study evaluated chimeric editing in gene editing, using a transgenic tobacco line and a CRISPR-Cas9 editing vector. It was found that chimeric editing primarily occurred during or after cell regeneration. Further transformation was conducted to enhance editing efficiency. The findings provide valuable information for understanding gene editing in plant cells using constitutively expressed CRISPR-Cas9 and gRNAs.
Review
Cell Biology
Heba M. Mansour, Aiman S. El-Khatib
Summary: This article reviews the pathogenesis of Parkinson's disease (PD) and its association with CRISPR/Cas9 gene editing technology. It introduces the kinases encoding genes related to PD and targeted control by CRISPR/Cas9 technology. It also discusses the off-target effects of the CRISPR/Cas9 system and how to address them, as well as the challenges and future directions in kinase research for PD.
AGEING RESEARCH REVIEWS
(2023)
Article
Biochemical Research Methods
Stefan J. Tekel, Nicholas Brookhouser, Kylie Standage-Beier, Xiao Wang, David A. Brafman
Summary: The introduction of transient reporters of editing enrichment (TREE) has enabled highly efficient single-base editing of human cells using a transient episomal fluorescent reporter, allowing for rapid generation of clonal editing efficiencies exceeding 80% in biallelic or multiplexed edited isogenic human pluripotent stem cell lines within approximately 3-4 weeks.
Review
Biology
Sayed Haidar Abbas Raza, Abdallah A. Hassanin, Sameer D. Pant, Sun Bing, Mahmoud Z. Sitohy, Sameh A. Abdelnour, Mashael Alhumaidi Alotaibi, Tahani Mohamed Al-Hazani, Ayman H. Abd El-Aziz, Gong Cheng, Linsen Zan
Summary: Significant progress has been made in genome editing applications in livestock production using tools such as CRISPR/Cas systems. These tools have the potential to improve animal performance and disease resistance, but also raise ethical and social concerns that must be addressed.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Chin-Kai Chuang, Wei-Ming Lin
Summary: Cas9-sgRNA is a key tool for genome editing, with its simplicity, unique RNA-guided DNA recognition activity, and independent nuclease activities. Different variants of Cas9, in combination with sgRNA, can achieve various effects on target site breakage. Additionally, engineered enzyme activities can be introduced using Cas9 derivatives, and methods for transient expression and biosafety have been developed to address off-target effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Veterinary Sciences
Evelyne Paris-Oller, Carmen Matas, Raquel Romar, Jordana S. Lopes, Joaquin Gadea, Sebastian Canovas, Pilar Coy
Summary: Embryo transfer (ET) technique has limitations in pig species compared to artificial insemination (AI), but offspring from ET have higher birth weight and different blood parameters. However, these differences become insignificant after 15 days. ET procedure is considered safe, but further studies are needed to determine its overall impact.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Public, Environmental & Occupational Health
Evelyne Paris-Oller, Cristina Soriano-Ubeda, Ramses Belda-Perez, Lucia Sarrias-Gil, Jordana S. Lopes, Analuce Canha-Gouveia, Joaquin Gadea, Luis Alberto Vieira, Francisco Alberto Garcia-Vazquez, Raquel Romar, Sebastian Canovas, Pilar Coy
Summary: This study aimed to investigate the long-term effects of adding reproductive fluids (RF) to culture media on piglet phenotype. The results showed that animals derived from in vitro fertilization and embryo culture had larger size at birth and faster growth compared to those from artificial insemination. However, this difference was diminished by the addition of RFs to the culture media. Similarly, small differences in hematological indices and glucose tolerance between animals derived from artificial insemination and in vitro fertilization tended to fade in the presence of RF.
JOURNAL OF DEVELOPMENTAL ORIGINS OF HEALTH AND DISEASE
(2022)
Article
Veterinary Sciences
Jordana S. Lopes, Cristina Soriano-Ubeda, Evelyne Paris-Oller, Sergio Navarro-Serna, Analuce Canha-Gouveia, Lucia Sarrias-Gil, Jose Joaquin Ceron, Pilar Coy
Summary: Assisted reproductive technologies play a significant role in the cattle industry, and the use of in vitro-derived embryos is increasing worldwide. This study compared the growth, development, and hematological and biochemical analytes of calves produced through in vivo production, standard in vitro production, and in vitro production with reproductive fluids added to the embryo culture media. The results showed that calves produced with reproductive fluids had growth and development patterns more similar to those born by artificial insemination than those produced with standard supplementation.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Reproductive Biology
Lauren M. Ataman-Millhouse, Pamela Monahan, Rebecca Willingham, Giulia Vigone, Mao Soulakis, Joaquin Gadea, Maria Jimenez-Movilla, Raquel Romar, Sebastian Canovas, Teresa K. Woodruff, Francesca E. Duncan
Summary: Master's programs in reproductive science are important educational mechanisms that contribute to the development of well-trained professionals in the field of reproductive health. Global examples of these programs provide valuable insights for expanding training programs worldwide.
BIOLOGY OF REPRODUCTION
(2022)
Article
Biochemistry & Molecular Biology
Sergio Navarro-Serna, Celia Pineiro-Silva, Chiara Luongo, John Parrington, Raquel Romar, Joaquin Gadea
Summary: This study evaluated the use of reversible inhibition of DNA replication to reduce mosaicism in embryos edited by CRISPR/Cas9. The findings showed a concentration-dependent DNA replication inhibition, leading to a decrease in mosaicism but a detrimental effect on embryo development. Balancing the use of this approach is necessary considering the impact on embryo development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Reproductive Biology
Sergio Navarro-Serna, Martxel Dehesa-Etxebeste, Celia Pinerio-Silva, Raquel Romar, Jordana S. Lopes, Adolfo Lopez de Munain, Joaquin Gadea
Summary: This study successfully generated a pig model of limb girdle muscular dystrophy type R1 (LGMDR1) by optimizing porcine oocyte electroporation. Electroporation, as a faster and easier method compared to microinjection, showed comparable rates of embryo development and mutation parameters.
Article
Agriculture, Dairy & Animal Science
Marta Moya-Jodar, Giulia Coppiello, Juan Roberto Rodriguez-Madoz, Gloria Abizanda, Paula Barlabe, Amaia Vilas-Zornoza, Asier Ullate-Agote, Chiara Luongo, Ernesto Rodriguez-Tobon, Sergio Navarro-Serna, Evelyne Paris-Oller, Maria Oficialdegui, Xonia Carvajal-Vergara, Laura Ordovas, Felipe Prosper, Francisco Alberto Garcia-Vazquez, Xabier L. Aranguren
Summary: The generation of organs using pluripotent stem cells combined with genetically engineered pig embryos through blastocyst complementation could provide a solution to the worldwide shortage of transplantable organs. In this study, a protocol to create vascular-disabled pig embryos using the CRISPR/Cas9 system was optimized, which could contribute to the generation of rejection-free humanized organs in pigs.
Article
Reproductive Biology
C. M. Picazo, C. Castano, P. Boveda, A. Toledano-Diaz, R. Velazquez, B. Pequeno, M. C. Esteso, J. Gadea, S. Villaverde-Morcillo, J. Cerdeira, J. Santiago-Moreno
Summary: This study compared the effects of slow freezing and vitrification on different cell types in canine and wild boar testicular tissue. The results showed that vitrification had higher cell viability and DNA integrity in dogs, while either method could be used in wild boars.
Article
Agriculture, Dairy & Animal Science
Celia Pineiro-Silva, Sergio Navarro-Serna, Ramses Belda-Perez, Joaquin Gadea
Summary: Lipofection treatment, which involves encapsulating the CRISPR/Cas9 system into vesicles and introducing them into cells, can be a simple and inexpensive method for producing genetically modified pigs. It has similar effects on embryo development and mutation rates as the electroporation technique when used in zona-pellucida-intact porcine oocytes. However, increasing the concentration of liposomes-CRISPR/Cas9 system complexes in the media has a detrimental effect on embryo development parameters, possibly indicating a toxic effect.
Article
Agriculture, Dairy & Animal Science
David Ramirez-Gonzalez, Angel Poto, Begona Peinado, Laura Almela, Sergio Navarro-Serna, Salvador Ruiz
Summary: A detailed study of fetal growth indices and umbilical artery flow rates in Murciano-Granadina goats has been performed using ultrasonography. The measurements were taken from 18 to 125 days post-breeding, and both fetal growth indices and blood flow parameters showed significant changes throughout the pregnancy period.
Editorial Material
Biochemistry & Molecular Biology
William V. Holt, Joaquin Gadea
Article
Agriculture, Dairy & Animal Science
Sara Crespo, Joaquin Gadea
Summary: The application of cloprostenol after farrowing improved fertility rates and reduced non-productive days in sows, leading to a faster rebreeding and higher pregnancy rate. The farrowing rate was significantly increased in the PGF2 alpha group, although no differences were found for litter size.
ITEA-INFORMACION TECNICA ECONOMICA AGRARIA
(2022)
Article
Veterinary Sciences
Adel K. Auth, Guy J. Hinnigan, Meredith A. Smith, Kathryn R. Owen
Summary: This study investigated the low-field magnetic resonance imaging (MRI) findings and lesion distribution in nonracehorses with MCP/MTPJ region pain. The results showed that soft tissue injuries were more common, with suspensory ligament branch injuries being the most frequent. In terms of osseous pathology, the subchondral bone (SCB) was most frequently affected, with the majority of lesions located on the dorsal aspect of the bone. The main limitations of the study were the lack of standardized diagnostic analgesia pattern and the absence of bilateral MRI sequences in all limbs.
JOURNAL OF EQUINE VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Humberto B. Magalhaes, Jose Antonio Dell'Aqua Jr, Igor F. Canisso
Summary: This study assessed luteolysis and side effects in jennies receiving standard horse-recommended doses of cloprostenol and dinoprost. The results showed that both dinoprost and cloprostenol effectively induced luteolysis, but with different side effects.
JOURNAL OF EQUINE VETERINARY SCIENCE
(2024)
