Article
Microbiology
Sabine Chapuy-Regaud, Marcel Miedouge, Florence Abravanel, Isabelle Da Silva, Marion Porcheron, Judith Fillaux, Chloe Dimeglio, Jacques Izopet
Summary: Three commercially available assays for SARS-CoV-2 antibodies were evaluated with varying sensitivity for different stages of infection. The assays showed correlation with neutralizing antibody titers, but discrepancies were observed in certain scenarios.
MICROBIOLOGY SPECTRUM
(2021)
Article
Virology
Julien Favresse, Julie Cadrobbi, Christine Eucher, Marc Elsen, Kim Laffineur, Jean-Michel Dogne, Jonathan Douxfils
Summary: This study evaluated the clinical performance of three anti-SARS-CoV-2 assays and found that their sensitivities were equivalent 14 days after symptom onset, with Elecsys showing better specificity. Detection of anti-SARS-CoV-2 antibodies before Day 14 was less clinically relevant.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Immunology
Olaf Nickel, Alexandra Rockstroh, Stephan Borte, Johannes Wolf
Summary: The study compares the diagnostic performance of three lateral flow assays in detecting antibodies after COVID-19 vaccination. The results suggest that these methods have reasonable application in short-term follow-up.
Article
Infectious Diseases
Sophie Owen, Christopher T. Williams, Gala Garrod, Alice J. Fraser, Stefanie Menzies, Lisa Baldwin, Lottie Brown, Rachel L. Byrne, Andrea M. Collins, Ana Cubas-Atienzar, Margaretha de Vos, Thomas Edwards, Camille Escadafal, Daniela M. Ferreira, Tom Fletcher, Angela Hyder-Wright, Grant A. Kay, Konstantina Kontogianni, Jenifer Mason, Elena Mitsi, Tim Planche, Jilian A. Sacks, Joseph Taylor, Stacy Todd, Caroline Tully, Luis E. Cuevas, Emily R. Adams
Summary: The evaluation of 12 LFAs showed varying sensitivity and specificity, with improved sensitivity for IgM/IgG over time since symptom onset. Compared to the EuroImmun IgG ELISA, LFAs exhibited different levels of sensitivity and specificity.
JOURNAL OF INFECTION
(2022)
Article
Virology
Arantxa Valdivia, Ignacio Torres, Victor Latorre, Clara Frances-Gomez, Josep Ferrer, Lorena Forque, Rosa Costa, Carlos Solano de la Asuncion, Dixie Huntley, Roberto Gozalbo-Rovira, Javier Buesa, Estela Gimenez, Jesus Rodriguez-Diaz, Ron Geller, David Navarro
Summary: This study assessed the performance of commercial lateral flow immunochromatographic assays (LFIC) in comparison with a SARS-CoV-2 neutralization pseudotyped assay for COVID-19 diagnosis, finding that LFIC sensitivity was high, especially for the Wondfo test. The intensity of the LFIC test bands was found to be correlated with neutralizing antibody titers, and these rapid immunoassays can potentially predict high SARS-CoV-2-S neutralizing antibody titers, providing reliable information for COVID-19 diagnosis.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Microbiology
Manuel Krone, Julia Guetling, Johannes Wagener, Thien-Tri Lam, Christoph Schoen, Ulrich Vogel, August Stich, Florian Wedekink, Joerg Wischhusen, Thomas Kerkau, Niklas Beyersdorf, Silvana Klingler, Simone Backes, Lars Doelken, Georg Gasteiger, Oliver Kurzai, Alexandra Schubert-Unkmeir
Summary: A study compared the performance of seven serological assays for COVID-19, with only one commercial immunoassay showing both sensitivity and specificity greater than 98%. Neutralizing antibodies were detected in a high percentage of COVID-19 survivors.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Infectious Diseases
Sarah Mafi, Sylvie Rogez, Jerome Darreye, Sophie Alain, Sebastien Hantz
Summary: The performance of SureScreen Diagnostics LFIA for SARS-CoV-2 antibody detection was evaluated and compared with three automated assays. The LFIA demonstrated good sensitivity and specificity, and its performance was not affected by different variants. This study confirms the clinical effectiveness of SureScreen Diagnostics LFIA for SARS-CoV-2 seroprevalence screening.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2023)
Article
Virology
Mickael Hirabidian, Laurence Bocket, Julie Demaret, Fanny Vuotto, Anthony Rabat, Karine Faure, Myriam Labalette, Didier Hober, Guillaume Lefevre, Enagnon Kazali Alidjinou
Summary: The performance of the ichromaTM COVID-19 nAb test in predicting serum neutralizing activity against SARS-CoV-2 variants was assessed in this study. The test showed good correlation with antibody levels and accurately predicted neutralization against different variants. It can be a useful tool for monitoring anti-SARS-CoV-2 immunity and rapidly predicting protection in individuals with significant antibody levels.
JOURNAL OF CLINICAL VIROLOGY
(2022)
Article
Immunology
Christina J. Atchison, Maya Moshe, Jonathan C. Brown, Matthew Whitaker, Nathan C. K. Wong, Anil A. Bharath, Rachel A. McKendry, Ara Darzi, Deborah Ashby, Christl A. Donnelly, Steven Riley, Paul Elliott, Wendy S. Barclay, Graham S. Cooke, Helen Ward
Summary: Self-administered LFIA is less sensitive than quantitative antibody tests, but its results correlate better with virus neutralization. Calibrated to the appropriate positivity threshold, LFIA rapid antibody self-testing may be useful in ongoing population surveillance.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Biophysics
Natalia-Maria Christopoulou, Despina P. Kalogianni, Theodore K. Christopoulos
Summary: Lateral flow immunoassays (LFIA) are widely used for low-cost, simple, and rapid point-of-need testing. A study found that adding water-soluble macromolecular crowding agents can enhance the assay sensitivity without compromising its simplicity. The researchers demonstrated the improved sensitivity in detecting SARS-CoV-2 and Strep-A antigen using commercially available LFIA devices. The addition of macromolecular crowding agents is complementary to other sensitivity enhancement strategies and can potentially be applied to various analytes and LFIA configurations.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Medicine, General & Internal
Wen-Yeh Hsieh, Cheng-Han Lin, Tzu-Ching Lin, Chao-Hsu Lin, Hui-Fang Chang, Chin-Hung Tsai, Hsi-Tien Wu, Chih-Sheng Lin
Summary: The COVID-19 pandemic, caused by SARS-CoV-2, poses a significant challenge to the global healthcare system. Early detection and treatment of infected cases are crucial, with rapid and cost-effective immunoassays like LFIA being utilized for quick and large-scale testing.
Article
Chemistry, Multidisciplinary
Lun Bian, Qiangqiang Fu, Zhuoheng Gan, Ze Wu, Yuchen Song, Yufeng Xiong, Fang Hu, Lei Zheng
Summary: In this study, a fluorescence-quenching lateral flow immunoassay (FQ-LFIA) was developed for rapid and quantitative detection of anti-SARS-CoV-2 neutralizing antibodies (NAbs) in human serum. The FQ-LFIA showed excellent sensitivity and accuracy, and has the potential to be an effective method for detecting anti-SARS-CoV-2 NAbs.
Article
Medicine, General & Internal
Philipp Girl, Katrin Zwirglmaier, Heiner von Buttlar, Roman Woelfel, Katharina Mueller
Summary: This study evaluated the performance of four commercially available tests for detecting SARS-CoV-2 neutralizing antibodies. The overall sensitivity and specificity varied among lateral flow assays, while surrogate ELISA tests showed higher sensitivity. All tests demonstrated the highest sensitivity when testing samples from fully vaccinated individuals.
FRONTIERS IN MEDICINE
(2022)
Review
Pathology
Jianfu J. Wang, Nan Zhang, Sarah A. Richardson, Jin V. Wu
Summary: The article discusses the importance of SARS-CoV-2 NAbs in combating the COVID-19 pandemic, available detection methods, challenges in developing a POCT, and product applications. It concludes that a widely accessible NAbs POCT is urgently needed.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2021)
Article
Infectious Diseases
Oskar Ekelund, Kim Ekblom, Sofia Somajo, Johanna Pattison-Granberg, Karl Olsson, Annika Petersson
Summary: The study investigated the performance of three high-throughput assays and one rapid lateral flow test for detecting antibodies against SARS-CoV-2. The results showed that all assays had high specificity, but sensitivity varied significantly among them.
INFECTIOUS DISEASES
(2021)
