期刊
JOURNAL OF CELL SCIENCE
卷 133, 期 14, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.246306
关键词
Sorting nexin; SNX4; SNX7; SNX30; Endosomes; Autophagy; ATG9A
类别
资金
- Biotechnology and Biological Sciences Research Council [BB/J002704/1]
- Biotechnology and Biological Sciences Research Council and Engineering and Physical Sciences Research Council through the BrisSynBio Synthetic Biology Research Centre [BB/L01386X1]
- Wellcome Trust [089928/Z/09/Z]
- BBSRC [BB/J002704/1] Funding Source: UKRI
- Wellcome Trust [089928/Z/09/Z] Funding Source: Wellcome Trust
The sorting nexins (SNXs) are a family of peripheral membrane proteins that direct protein trafficking decisions within the endocytic network. Emerging evidence in yeast and mammalian cells implicates a subgroup of SNXs in selective and non-selective forms of autophagy. Using siRNA and CRISPR-Cas9, we demonstrate that the SNX-BAR protein SNX4 is needed for efficient LC3 (also known as MAP1LC3) lipidation and autophagosome assembly in mammalian cells. SNX-BARs exist as homo- and hetero-dimers, and we show that SNX4 forms functional heterodimers with either SNX7 or SNX30 that associate with tubulovesicular endocytic membranes. Detailed image-based analysis during the early stages of autophagosome assembly reveals that SNX4-SNX7 is an autophagy-specific SNX-BAR heterodimer, required for efficient recruitment and/or retention of core autophagy regulators at the nascent isolation membrane. SNX4 partially colocalises with juxtanuclear ATG9A-positive membranes, with our data linking the autophagy defect upon SNX4 disruption to the mis-trafficking and/or retention of ATG9A in the Golgi region. Taken together, our findings show that the SNX4-SNX7 heterodimer coordinates ATG9A trafficking within the endocytic network to establish productive autophagosome assembly sites, thus extending knowledge of SNXs as positive regulators of autophagy.
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