Article
Immunology
Jesse O'Shea, Mark R. Prausnitz, Nadine Rouphael
Summary: The dissolvable microneedle patches offer multiple advantages for vaccination, including improved immunogenicity, dose-sparing effects, cost reduction, waste reduction, and simplified supply chain. They have great potential to improve vaccination globally and save lives in the context of COVID-19 and future pandemic outbreaks.
Article
Pharmacology & Pharmacy
Lyndsey E. Moore, Sonja Vucen, Anne C. Moore
Summary: This review discusses the recent advances in microneedle patches technology for drug delivery and sampling, with a focus on materials and fabrication methods of dissolvable microneedles. Through extensive literature review, it summarizes the application of dissolvable microneedles in drugs and vaccines, as well as the diversity in production methods.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Article
Chemistry, Multidisciplinary
Song Li, Dengning Xia, Mark R. Prausnitz
Summary: The study introduces biphasic dissolvable microneedle patches which enhance efficient drug delivery to the skin, reducing drug residue and waste.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Instruments & Instrumentation
Yoo Chun Kim, Jeong Woo Lee, E. Stein Esser, Haripriya Kalluri, Jessica C. Joyce, Richard W. Compans, Ioanna Skountzou, Mark R. Prausnitz
Summary: The study developed a method to incorporate lyophilized influenza vaccine into microneedle patches, enabling stable processing and storage, as well as strong immune responses in a murine model.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Luca Casula, Rosa Pireddu, Maria Cristina Cardia, Elena Pini, Donatella Valenti, Michele Schlich, Chiara Sinico, Salvatore Marceddu, Nina Dragicevic, Anna Maria Fadda, Francesco Lai
Summary: Combining microneedle arrays with nanosuspensions can enhance the skin delivery of water insoluble drugs by temporarily disrupting the stratum corneum.
Review
Pharmacology & Pharmacy
Megan McNamee, Shuyi Wong, Owen Guy, Sanjiv Sharma
Summary: Microneedle fabrication was developed in the 1970s for painless transdermal drug delivery. In the past two decades, there has been significant research and investment in this area, with the emergence of SARS-CoV-2 and other vaccines accelerating their application for in vivo intradermal vaccine delivery. Microneedle arrays can be fabricated in different shapes, geometries, formats, and materials.
EXPERT OPINION ON DRUG DELIVERY
(2023)
Review
Multidisciplinary Sciences
Yanni Wang, Gehua Ma, Guangzhi Gao, Ji Tao, Wenzhao Cao, Haohao Sun, Fengsen Ma, Yilong Zhang, Yen Wei, Mei Tian
Summary: The emergence of microneedle arrays as a novel drug delivery approach has addressed the challenges of traditional methods. Advanced imaging technologies can provide valuable assistance in evaluating and developing these microneedle arrays, leading to their potential application in medicine and cosmetics.
Article
Pharmacology & Pharmacy
Deepanjan Datta, Girdhari Roy, Prashant Garg, Venkata Vamsi Krishna Venuganti
Summary: A rapidly dissolving polymeric microneedle ocular patch (MOP) was fabricated to effectively deliver cyclosporine A (CsA) within the cornea.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mohammad Reza Zare, Mohammad Khorram, Sajjad Barzegar, Bahador Sarkari, Qasem Asgari, Samad Ahadian, Kamiar Zomorodian
Summary: Cutaneous leishmaniasis is a significant public health problem caused by different species of Leishmania parasites. A dissolvable microneedle patch was developed for transdermal delivery of Amphotericin B, showing potential as an efficient and clinically translational approach for treating CL.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Nanoscience & Nanotechnology
Ying Zheng, Rui Ye, Xia Gong, Jingbo Yang, Bin Liu, Yunsheng Xu, Gang Nie, Xi Xie, Lelun Jiang
Summary: COVID-19 poses a serious threat to public health, and a wearable iontophoresis-driven microneedle (MN) patch is developed for active and efficient transdermal vaccine delivery. The patch consists of polyacrylamide/chitosan hydrogels with excellent properties, and the delivery efficiency is greatly improved by the synergistic effect of MN puncture and iontophoresis. In vitro and in vivo studies demonstrate controlled delivery of ovalbumin and a stronger immune response compared to traditional intramuscular injection, with minimal biosafety concerns. This low-cost and user-friendly delivery system has great potential for vaccine self-administration at home.
MICROSYSTEMS & NANOENGINEERING
(2023)
Article
Biophysics
Xiaoli Guo, Tingting Zhu, Xixi Yu, Xin Yi, Longhao Li, Xiaoying Qu, Zhe Zhang, Yuanping Hao, Wanchun Wang
Summary: Oral inflammatory disease (OID), particularly oral ulcers, is a common oral lesion that affects quality of life and can lead to oral cancer. Traditional treatment methods for oral ulcers, such as localized hormone injections, can cause pain and fear. To address this, a soluble hyaluronic acid (HA) microneedle patch containing betamethasone 21-phosphate sodium (BSP) and betamethasone 17,21-dipropionate (BDP) was developed. The patch showed mechanical strength to penetrate the mucosa and released BSP and BDP into the ulcer base rapidly. It exhibited wound healing-promoting and anti-inflammatory effects, providing a new prospect for oral ulcer treatment.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Seung-Hwan Joo, Jaehyun Kim, Juhyeong Hong, Shayan Fakhraei Lahiji, Yong-Hee Kim
Summary: Advancements in micro-resolution 3D printers have enabled the development of complex drug delivery platforms. A novel self-locking microneedle (MN) based on a projection micro-stereolithography 3D printer is presented, which improves the accuracy of skin penetration. The self-locking MNs show significantly improved dose efficacy compared with intratumoral injection in a melanoma mouse model.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Dan Wu, Xin Shou, Yunru Yu, Xiaocheng Wang, Guopu Chen, Yuanjin Zhao, Lingyun Sun
Summary: A novel photo-thermally dissolvable hyaluronic acid microneedle patch with biologics encapsulation has been developed for the treatment of psoriasis. The microneedles possess suitable mechanical strength and biocompatibility, allowing for minimal immune response upon skin puncture. The rapid melting and release of encapsulated IL-17 mAbs under near-infrared light irradiation provide an effective anti-inflammatory effect around psoriasis lesions. This multifunctional microneedle patch effectively reduces psoriasis-like skin inflammation in an animal model and offers a safer and more effective strategy for psoriasis treatment and other chronic inflammatory autoimmune diseases.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Immunology
Muhammet Karakavuk, Huseyin Can, Aytul Gul, Aysu Degirmenci Doskaya, Sedef Erkunt Alak, Cemal Un, Adnan Yuksel Guruz, Mert Doskaya
Summary: The study demonstrated that the pcDNA 3.3 vector encoding GRA8, with or without CpG and Liposome, can induce strong cellular and humoral immune responses, providing strong protection against a mouse model of chronic toxoplasmosis.
MICROBIAL PATHOGENESIS
(2021)
Article
Chemistry, Analytical
Zulcaif, Nadiah Zafar, Asif Mahmood, Rai Muhammad Sarfraz, Abdelhamid Elaissari
Summary: dissolvable microneedle patches (dMNPs) were developed as a novel tool for improved systemic delivery of Simvastatin, showing higher bioavailability and good biocompatibility.
Article
Chemistry, Applied
Timothee Aboua Okpekon, Faustin Aka Kabran, Venance Martial Say, Laurent Evanno, Alexandre Maciuk, Philippe Loiseau, Pierre Champy, Bruno Figadere
Summary: Bioassay guided fractionation of the stem barks of Isolona cooperi led to the isolation of a new lactone and two known compounds. The new compound showed high antiprotozoal activities against Leishmania donovani and L. major promastigotes, indicating potential for further research.
NATURAL PRODUCT RESEARCH
(2021)
Article
Chemistry, Medicinal
Marc-Antoine Bazin, Sandrine Cojean, Fabrice Pagniez, Guillaume Bernadat, Christian Cave, Isabelle Ourliac-Garnier, Marie-Renee Nourrisson, Cathy Morgado, Carine Picot, Olivier Leclercq, Blandine Baratte, Thomas Robert, Gerald F. Spath, Najma Rachidi, Stephane Bach, Philippe M. Loiseau, Patrice Le Pape, Pascal Marchand
Summary: Leishmaniasis is a severe public health problem with an estimated prevalence of 12 million cases. The fatal Visceral Leishmaniasis (VL) is declared as a new emerging disease in Europe in 2012. New drugs with new mechanism of action are urgently needed to replace current treatment options which are toxic, costly or lead to parasite resistance.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Ana Luiza P. Queiroz, Marisa Rodrigues, Jacek Zeglinski, Abina M. Crean, Mafalda Cruz Sarraguca, Sonja Vucen
Summary: The study successfully determined the phase purity of pharmaceutical co-crystals using Multivariate Curve Resolution (MCR) combined with in-silico coformer screening and experimental co-crystallization methods.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
Khaled ElKassas, Krishnakumar Chullipalliyalil, Michael McAuliffe, Sonja Vucen, Abina Crean
Summary: This study explores the use of UV-excited fluorescence spectroscopy as an alternative method for quantifying the reconstitution time of lyophilised formulations. By analyzing spectrographic information and using PCA, the variability of reconstitution time measurements is significantly reduced compared to traditional visual methods.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Materials Science, Paper & Wood
Ana Luiza P. Queiroz, Brian M. Kerins, Jayprakash Yadav, Fatma Farag, Waleed Faisal, Mary Ellen Crowley, Simon E. Lawrence, Humphrey A. Moynihan, Anne-Marie Healy, Sonja Vucen, Abina M. Crean
Summary: By analyzing Raman spectra of 30 commercial batches of MCC, models were developed to determine the crystallinity index, showing good predictive power for different probes. The development of the PLS model substantially reduced analysis time, proposing a general reference amorphous spectrum for each instrument.
Article
Pharmacology & Pharmacy
Lyndsey E. Moore, Sonja Vucen, Anne C. Moore
Summary: This review discusses the recent advances in microneedle patches technology for drug delivery and sampling, with a focus on materials and fabrication methods of dissolvable microneedles. Through extensive literature review, it summarizes the application of dissolvable microneedles in drugs and vaccines, as well as the diversity in production methods.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Review
Biochemistry & Molecular Biology
Philippe M. Loiseau, Kaluvu Balaraman, Gillian Barratt, Sebastien Pomel, Remy Durand, Frederic Frezard, Bruno Figadere
Summary: This review documents the development of the 2-substituted quinoline series as a potential treatment for leishmaniasis. The series has shown promising antileishmanial activity with low toxicity, and attempts have been made to establish structure-activity relationships and determine the mechanism of action. The molecules in this series appear to act on multiple targets, including the immune system, which may explain the lack of drug resistance observed after in vitro drug pressure. Nanotechnology strategies and mechanistic targeting approaches may enhance the activity of these drugs.
Article
Pharmacology & Pharmacy
Guilherme S. Ramos, Virginia M. R. Vallejos, Gabriel S. M. Borges, Raquel M. Almeida, Izabela M. Alves, Marta M. G. Aguiar, Christian Fernandes, Pedro P. G. Guimaraes, Ricardo T. Fujiwara, Philippe M. Loiseau, Lucas A. M. Ferreira, Frederic Frezard
Summary: This study developed a formulation of PEGylated liposomal AmB and compared its efficacy to AmBisome (R) in a murine model of cutaneous leishmaniasis (CL). The results showed that the PEGylated formulation significantly reduced lesion growth and parasite load in the treatment of CL, both through parenteral and oral routes.
Article
Immunology
Sarah Marshall, Anne C. Moore, Aoife Fleming, Laura J. Sahm
Summary: The aim of this study was to design, develop, and evaluate a theory- and evidence-based intervention to improve HPV and HPV vaccine knowledge and intention to vaccinate among parent-daughter dyads. An online behavioral intervention was developed and found to be effective in improving knowledge and intention to vaccinate. Participants found the intervention interesting and useful.
Article
Pharmacology & Pharmacy
Juliane S. Lanza, Virginia M. R. Vallejos, Guilherme S. Ramos, Ana Carolina B. de Oliveira, Cynthia Demicheli, Luis Rivas, Sebastien Pomel, Philippe M. Loiseau, Frederic Frezard
Summary: This study evaluates the effectiveness of nanoassemblies made from antimony(V) complexes with octanoyl-N-methylglucamide (SbL8) or decanoyl-N-methylglucamide (SbL10) in targeting infection sites in visceral and cutaneous leishmaniases. The results show that these nanoassemblies are stable at neutral pH and effectively accumulate in macrophages and the liver. SbL10 exhibits higher activity against intramacrophage Leishmania amastigotes and can be further enhanced with co-incorporated miltefosine. In vivo, NanoSb demonstrates high antileishmanial activity in murine models of VL and CL. This study supports the potential of NanoSb as a better delivery system for antimony and co-incorporated drugs compared to conventional antimonial Glucantime (R).
Review
Immunology
Ines Co-Rives, Ann Ying-An Chen, Anne C. Moore
Summary: The advantages of skin-based vaccination include strong immunity, dose-sparing, and easy administration. Although it is more conventionally used in veterinary medicine, skin-based vaccination of pigs is highly relevant due to their similarities to humans and the zoonotic diseases they carry. However, there is a lack of consistent terminology and research on the underlying mechanisms and breadth of responses. Further research is needed to develop enhanced skin vaccine strategies for pigs, other animals, and humans.
Article
Immunology
Kyle Malone, Jennifer A. Shearer, Christian Waeber, Anne C. Moore
Summary: Fingolimod improves neurobehavioral recovery in stroke models and increases the frequency of Treg cells. However, it also increases the resistance of CD4(+) effector cells to Treg suppression. This complexity may explain the inconsistent improvement in functional recovery observed in experimental brain ischemia.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Immunology
Kyle Malone, Jennifer A. Shearer, John M. Williams, Anne C. Moore, Tom Moore, Christian Waeber
Summary: The administration of rPSG1-Fc improved functional recovery in post-ischaemic mice without impacting infarct size. Improved outcome was associated with a modulation of the cytokine-secreting phenotype of CD4+ and CD8+ T cells towards a more regulatory phenotype, as well as reduced activation of microglia. This establishes proof-of-concept of rPSG1-Fc as a potential stroke immunotherapy.
BRAIN, BEHAVIOR, & IMMUNITY - HEALTH
(2022)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)