期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 21, 期 15, 页码 -出版社
MDPI
DOI: 10.3390/ijms21155217
关键词
circRNA expression profiles; antral follicular atresia; granulosa cell apoptosis
资金
- National 13th Five-Year Plan Key R&D Program of China [2017YFD0501902, 2018YFD0501000]
- National Natural Science Foundation of China (NSFC) [31902157]
- Guangdong Provincial Education Department Talent Project [2017KQNCX013]
- Guangdong Provincial Promotion Project on Preservation and Utilization of Local Breed of Livestock and Poultry
- Hong Kong Scholars Program
Circular RNAs (circRNAs) are thought to play essential roles in multiple biological processes, including apoptosis, an important process in antral follicle atresia. We aimed to investigate the potential involvement of circRNAs in granulosa cell apoptosis and thus antral follicle atresia. CircRNA expression profiles were generated from porcine granulosa cells isolated from healthy antral (HA) and atretic antral (AA) follicles. Over 9632 circRNAs were identified, of which 62 circRNAs were differentially expressed (DE-circRNAs). Back-splicing, RNase R resistance, and stability of DE-circRNAs were validated, and miRNA binding sites and related target genes were predicted. Two exonic circRNAs with low false discovery rate (FDR) high fold change, miRNA binding sites, and relevant biological functions-circ_CBFA2T2 and circ_KIF16B-were selected for further characterization. qRT-PCR and linear regression analysis confirmed expression and correlation of the targeted genes-the antioxidant geneGCLC(potential target of circ_CBFA2T2) and the apoptotic geneTP53(potential target of circ_KIF16B). Increased mRNA content ofTP53in granulosa cells of AA follicles was further confirmed by strong immunostaining of both p53 and its downstream target pleckstrin homology like domain family a member 3 (PHLDA3) in AA follicles compared to negligible staining in granulosa cells of HA follicles. Therefore, we concluded that aberrantly expressed circRNAs presumably play a potential role in antral follicular atresia.
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